SlideShare ist ein Scribd-Unternehmen logo
1 von 18
Downloaden Sie, um offline zu lesen
ISOLATION OF A NOVEL
MYCOBACTERIOPHAGE
Lizbeth Pérez Castro
Javier Zavala Ayala
Mónica Del Moral
Félix Valles
WHAT IS A BACTERIOPHAGE?
• A bacteriophage is any virus that infects any bacterium.
• Mycobacteriophages are viruses that infect mycobacteria.
• All mycobacteriophages are bacteriophages, but not all
bacteriophages are mycobacteriophages.
• Found in different bacterial colonies.
• Mostly found in the soil and sea water.
• Resistant to antibiotics.
BACTERIAS FOR HOSTING
1. M. smegmatis (Smeg)
• fast growing rate and non-pathogenic behaviour
• Aerobic organisms
• Found in water, soil and plants
2. Bacillus
• Obligate aerobes or facultative anaerobes
• Well understood- used as a model for differentiation, gene regulation, and cell
cycle events in bacteria.
• Common in soil
 Both are:
 Gram positive
 Acid fast bacteria
 Morfology- rodshape
BACTERIOPHAGE STRUCTURE
• Phages consist of 3 basic
components:
• Capsid-contains the genetic
material
• Genetic material- DNA
• Tail-serves to attach to bacteria
and DNA passes through tail and
into bacterium
http://en.wikipedia.org/wiki/Bacteriophage
PHAGE LIFE CYCLES
• Temperate-Lisogenic:
• Replicate and lyse or dormant state- incorporate its genetic material into DNA of
host.
• Virulent-Lytic:
• Straightforward cycle by lysing all bacteria they infect.
http://biomedfrontiers.org/infection-2014-3-28/
PROCEDURE
httpwww.teachthechil
drenwell.comscience.h
tml
http://www.exportersin
dia.com/swastikaelectr
ic/products.htm
http://quizlet.com/6760545/lab-3-streak-plate-
technique-flash-cards/
Soil
Enrichment
Filtration
Purification
HTLTen Plate Preparation
MY BACTERIOPHAGE
• Name: Nitidusvenutus
• Located in: Carretera 943 Sector Los
Chinos, Gurabo
• Coordinates: 18º 16’ 7’ N, 65º 58’ 32’ W
• Collected: February 24, 2014
• It was found in a rural area next to cow
feces.
LOCATION
MY BACTERIOPHAGE
RESULTS
http://faculty.plattsburgh.edu/don
ald.slish/lambdatitre.html
MY BACTERIOPHAGE: MUSAMODEL
• Name- Musamodel
• Located in: 1-9 calle
Barcelona, Urb.Terralinda, Caguas P.R
• Coordinates: 88˚14’6”N , 66˚2’39”W
• Collected: March 16, 2014
• Time: 7:51pm
• Temperature: 79˚
• About 3 inches deep and 1 foot close
to concrete
• Found next to a plantain tree
• Soil was moist and loose
LOCATION
SAMPLES COLLECTED
1st 2nd 3rd 4th 5th 6th 7th
Date 2/6/14 2/18/14 2/24/14 3/3/14 3/10/14 3/16/14 3/16/14
Time 7:53 am 7:06 am 9:55 pm 9:38 am 6:26 am 7:45 pm 7:51 pm
Coordinates 18˚14’6”N,
66˚2’40”W
18˚14’13”N,
66˚2’35”W
18˚14’7”N,
66˚2’39”W
18˚14’18”N,
66˚2’42”W
18˚14’7”N,
66˚2’40”W
18˚14’6”N,
66˚2’39”W
18˚14’17”N,
66˚2’42”W
Temperature 75˚ 75˚ 79˚ 81˚ 73˚ 79˚ 79˚
Soil
description
Moist, 3
inches deep
Moist, 1 inch
deep
Moist, 1
inch deep
Moist, with
moss, 1 inch
deep
Moist, 1
inch
Moist,
loose, 5
inches
deep
Moist, loose,
3 inches
deep
Site
description
Urban, 5 feet
close to
concrete
Urban, 1 foot
from
concrete
Urban, 2
feet from
concrete
Urban, ½
foot from
concrete
Urban, 3
feet away
from
concrete
Urban, 1
foot close
to
concrete
Next to a
plantain
plant, 1 foot
close to
concrete
RESULTS
CONCLUSION
• Two mycobacteriophages were found:
1. Nitidusvenutus
a. located in Carretera 943 Sector Los Chinos, Gurabo
b. ……. Were completed
2. Musamodel
a. located in 1-9 calle Barcelona, Urb. Terralinda, Caguas P.R
b. Up to now, we believe it has a lysogenic life cycle because of turbidness
c. three purifications were completed
FUTURE PLANS
• Nitidusvenutus
• Musamodel;
• 3rd purification- to have more isolated plaques and measure diameter
• Spot test
• Make phage stocks-
• Empirical test
• 10 plate preparation
• Analyze
• Bioinformatics and sequencing
ACKNOWLEDGEMENT
• Dr. Michael Rubin
• Juan Apiz
• Eduardo Correa
• Gustavo Martínez
• Giovanni Cruz

Más contenido relacionado

Ähnlich wie Phage presentation spring 2014

mashroom production
 mashroom production mashroom production
mashroom productionmeshalejaz
 
Plant genome project, tahira ali rai
Plant genome project, tahira ali raiPlant genome project, tahira ali rai
Plant genome project, tahira ali raiTahira Rai
 
Analysis of potential vectors of Serratia marcescens as a Caretta caretta nes...
Analysis of potential vectors of Serratia marcescens as a Caretta caretta nes...Analysis of potential vectors of Serratia marcescens as a Caretta caretta nes...
Analysis of potential vectors of Serratia marcescens as a Caretta caretta nes...BHIConservancy
 
05 Malaria I.pdf
05 Malaria I.pdf05 Malaria I.pdf
05 Malaria I.pdfDEEPSAB
 
Ecological diversity of Microorganisms
Ecological diversity of MicroorganismsEcological diversity of Microorganisms
Ecological diversity of Microorganismsmisbah malik
 
Nobel prize in medicine and physiology 2017
Nobel prize in medicine and physiology 2017Nobel prize in medicine and physiology 2017
Nobel prize in medicine and physiology 2017Binyamin Butt
 
4.monica 13.felix phages report corrected
4.monica 13.felix phages report corrected4.monica 13.felix phages report corrected
4.monica 13.felix phages report correctedfelixjvalles
 

Ähnlich wie Phage presentation spring 2014 (19)

Paramecium
ParameciumParamecium
Paramecium
 
Chapter 1 part 1 microbial world and you
Chapter 1 part 1 microbial world and youChapter 1 part 1 microbial world and you
Chapter 1 part 1 microbial world and you
 
Phage hunters 2014
Phage hunters 2014Phage hunters 2014
Phage hunters 2014
 
Phage hunters 2014 (2)
Phage hunters 2014 (2)Phage hunters 2014 (2)
Phage hunters 2014 (2)
 
Phage hunters 2014
Phage hunters 2014Phage hunters 2014
Phage hunters 2014
 
Leptospira azadi
Leptospira azadiLeptospira azadi
Leptospira azadi
 
Phage hunters 2014 (2)
Phage hunters 2014 (2)Phage hunters 2014 (2)
Phage hunters 2014 (2)
 
Bacteria
BacteriaBacteria
Bacteria
 
mashroom production
 mashroom production mashroom production
mashroom production
 
Plant genome project, tahira ali rai
Plant genome project, tahira ali raiPlant genome project, tahira ali rai
Plant genome project, tahira ali rai
 
Normal Skin Flora
Normal Skin FloraNormal Skin Flora
Normal Skin Flora
 
Analysis of potential vectors of Serratia marcescens as a Caretta caretta nes...
Analysis of potential vectors of Serratia marcescens as a Caretta caretta nes...Analysis of potential vectors of Serratia marcescens as a Caretta caretta nes...
Analysis of potential vectors of Serratia marcescens as a Caretta caretta nes...
 
Phages report 2
Phages report 2Phages report 2
Phages report 2
 
05 Malaria I.pdf
05 Malaria I.pdf05 Malaria I.pdf
05 Malaria I.pdf
 
Ecological diversity of Microorganisms
Ecological diversity of MicroorganismsEcological diversity of Microorganisms
Ecological diversity of Microorganisms
 
Nobel prize in medicine and physiology 2017
Nobel prize in medicine and physiology 2017Nobel prize in medicine and physiology 2017
Nobel prize in medicine and physiology 2017
 
Vasanth's leptospira
Vasanth's leptospiraVasanth's leptospira
Vasanth's leptospira
 
4.monica 13.felix phages report corrected
4.monica 13.felix phages report corrected4.monica 13.felix phages report corrected
4.monica 13.felix phages report corrected
 
Ch 2 biological classification
Ch 2 biological classificationCh 2 biological classification
Ch 2 biological classification
 

Mehr von felixjvalles

Reflective essay.docx 2
Reflective essay.docx 2Reflective essay.docx 2
Reflective essay.docx 2felixjvalles
 
Language workshop mar 7, 2014.htm
Language workshop mar 7, 2014.htmLanguage workshop mar 7, 2014.htm
Language workshop mar 7, 2014.htmfelixjvalles
 
Language workshop feb 6, 2014.htm
Language workshop feb 6, 2014.htmLanguage workshop feb 6, 2014.htm
Language workshop feb 6, 2014.htmfelixjvalles
 
Language workshop feb 6, 2014.htm
Language workshop feb 6, 2014.htmLanguage workshop feb 6, 2014.htm
Language workshop feb 6, 2014.htmfelixjvalles
 
Carbon dots poem rise
Carbon dots poem riseCarbon dots poem rise
Carbon dots poem risefelixjvalles
 
Carbon dots poem rise
Carbon dots poem riseCarbon dots poem rise
Carbon dots poem risefelixjvalles
 
Creative writting rise
Creative writting riseCreative writting rise
Creative writting risefelixjvalles
 
Reflective essay rise
Reflective essay riseReflective essay rise
Reflective essay risefelixjvalles
 
5th summary rise slideshare
5th summary rise slideshare5th summary rise slideshare
5th summary rise slidesharefelixjvalles
 
5th summary rise slideshare
5th summary rise slideshare5th summary rise slideshare
5th summary rise slidesharefelixjvalles
 
6th summary rise slideshare
6th summary rise slideshare6th summary rise slideshare
6th summary rise slidesharefelixjvalles
 
Mn ps final oral presentaion rise corrected
Mn ps  final oral presentaion rise correctedMn ps  final oral presentaion rise corrected
Mn ps final oral presentaion rise correctedfelixjvalles
 
Mn ps final oral presentaion rise corrected
Mn ps  final oral presentaion rise correctedMn ps  final oral presentaion rise corrected
Mn ps final oral presentaion rise correctedfelixjvalles
 
Mn ps final oral presentaion rise corrected
Mn ps  final oral presentaion rise correctedMn ps  final oral presentaion rise corrected
Mn ps final oral presentaion rise correctedfelixjvalles
 
Curriculum vitae rise
Curriculum vitae riseCurriculum vitae rise
Curriculum vitae risefelixjvalles
 
Curriculum vitae rise
Curriculum vitae riseCurriculum vitae rise
Curriculum vitae risefelixjvalles
 
Curriculum vitae rise
Curriculum vitae riseCurriculum vitae rise
Curriculum vitae risefelixjvalles
 

Mehr von felixjvalles (20)

Reflective essay.docx 2
Reflective essay.docx 2Reflective essay.docx 2
Reflective essay.docx 2
 
Language workshop
Language workshopLanguage workshop
Language workshop
 
Language workshop mar 7, 2014.htm
Language workshop mar 7, 2014.htmLanguage workshop mar 7, 2014.htm
Language workshop mar 7, 2014.htm
 
Language workshop feb 6, 2014.htm
Language workshop feb 6, 2014.htmLanguage workshop feb 6, 2014.htm
Language workshop feb 6, 2014.htm
 
Language workshop feb 6, 2014.htm
Language workshop feb 6, 2014.htmLanguage workshop feb 6, 2014.htm
Language workshop feb 6, 2014.htm
 
Reflective essay
Reflective essayReflective essay
Reflective essay
 
Carbon dots poem rise
Carbon dots poem riseCarbon dots poem rise
Carbon dots poem rise
 
Carbon dots poem rise
Carbon dots poem riseCarbon dots poem rise
Carbon dots poem rise
 
Creative writting rise
Creative writting riseCreative writting rise
Creative writting rise
 
Reflective essay rise
Reflective essay riseReflective essay rise
Reflective essay rise
 
5th summary rise slideshare
5th summary rise slideshare5th summary rise slideshare
5th summary rise slideshare
 
5th summary rise slideshare
5th summary rise slideshare5th summary rise slideshare
5th summary rise slideshare
 
6th summary rise slideshare
6th summary rise slideshare6th summary rise slideshare
6th summary rise slideshare
 
Mn ps manuscript
Mn ps manuscriptMn ps manuscript
Mn ps manuscript
 
Mn ps final oral presentaion rise corrected
Mn ps  final oral presentaion rise correctedMn ps  final oral presentaion rise corrected
Mn ps final oral presentaion rise corrected
 
Mn ps final oral presentaion rise corrected
Mn ps  final oral presentaion rise correctedMn ps  final oral presentaion rise corrected
Mn ps final oral presentaion rise corrected
 
Mn ps final oral presentaion rise corrected
Mn ps  final oral presentaion rise correctedMn ps  final oral presentaion rise corrected
Mn ps final oral presentaion rise corrected
 
Curriculum vitae rise
Curriculum vitae riseCurriculum vitae rise
Curriculum vitae rise
 
Curriculum vitae rise
Curriculum vitae riseCurriculum vitae rise
Curriculum vitae rise
 
Curriculum vitae rise
Curriculum vitae riseCurriculum vitae rise
Curriculum vitae rise
 

Último

Unleashing Datas Potential - Mastering Precision with FCO-IM
Unleashing Datas Potential - Mastering Precision with FCO-IMUnleashing Datas Potential - Mastering Precision with FCO-IM
Unleashing Datas Potential - Mastering Precision with FCO-IMMarco Wobben
 
STOCK PRICE ANALYSIS Furkan Ali TASCI --.pptx
STOCK PRICE ANALYSIS  Furkan Ali TASCI --.pptxSTOCK PRICE ANALYSIS  Furkan Ali TASCI --.pptx
STOCK PRICE ANALYSIS Furkan Ali TASCI --.pptxFurkanTasci3
 
Data Collection from Social Media Platforms
Data Collection from Social Media PlatformsData Collection from Social Media Platforms
Data Collection from Social Media PlatformsMahmoud Yasser
 
Air Con Energy Rating Info411 Presentation.pdf
Air Con Energy Rating Info411 Presentation.pdfAir Con Energy Rating Info411 Presentation.pdf
Air Con Energy Rating Info411 Presentation.pdfJasonBoboKyaw
 
Microeconomic Group Presentation Apple.pdf
Microeconomic Group Presentation Apple.pdfMicroeconomic Group Presentation Apple.pdf
Microeconomic Group Presentation Apple.pdfmxlos0
 
Data Analytics Fundamentals: data analytics types.potx
Data Analytics Fundamentals: data analytics types.potxData Analytics Fundamentals: data analytics types.potx
Data Analytics Fundamentals: data analytics types.potxEmmanuel Dauda
 
STOCK PRICE ANALYSIS Furkan Ali TASCI --.pptx
STOCK PRICE ANALYSIS  Furkan Ali TASCI --.pptxSTOCK PRICE ANALYSIS  Furkan Ali TASCI --.pptx
STOCK PRICE ANALYSIS Furkan Ali TASCI --.pptxFurkanTasci3
 
Brain Tumor Detection with Machine Learning.pptx
Brain Tumor Detection with Machine Learning.pptxBrain Tumor Detection with Machine Learning.pptx
Brain Tumor Detection with Machine Learning.pptxShammiRai3
 
The market for cross-border mortgages in Europe
The market for cross-border mortgages in EuropeThe market for cross-border mortgages in Europe
The market for cross-border mortgages in Europe321k
 
Enabling GenAI Breakthroughs with Knowledge Graphs
Enabling GenAI Breakthroughs with Knowledge GraphsEnabling GenAI Breakthroughs with Knowledge Graphs
Enabling GenAI Breakthroughs with Knowledge GraphsNeo4j
 
Neo4j Graph Summit 2024 Workshop - EMEA - Breda_and_Munchen.pdf
Neo4j Graph Summit 2024 Workshop - EMEA - Breda_and_Munchen.pdfNeo4j Graph Summit 2024 Workshop - EMEA - Breda_and_Munchen.pdf
Neo4j Graph Summit 2024 Workshop - EMEA - Breda_and_Munchen.pdfNeo4j
 
Bengaluru Tableau UG event- 2nd March 2024 Q1
Bengaluru Tableau UG event- 2nd March 2024 Q1Bengaluru Tableau UG event- 2nd March 2024 Q1
Bengaluru Tableau UG event- 2nd March 2024 Q1bengalurutug
 
How to Build an Experimentation Culture for Data-Driven Product Development
How to Build an Experimentation Culture for Data-Driven Product DevelopmentHow to Build an Experimentation Culture for Data-Driven Product Development
How to Build an Experimentation Culture for Data-Driven Product DevelopmentAggregage
 
Neo4j_Jesus Barrasa_The Art of the Possible with Graph.pptx.pdf
Neo4j_Jesus Barrasa_The Art of the Possible with Graph.pptx.pdfNeo4j_Jesus Barrasa_The Art of the Possible with Graph.pptx.pdf
Neo4j_Jesus Barrasa_The Art of the Possible with Graph.pptx.pdfNeo4j
 
Prediction Of Cryptocurrency Prices Using Lstm, Svm And Polynomial Regression...
Prediction Of Cryptocurrency Prices Using Lstm, Svm And Polynomial Regression...Prediction Of Cryptocurrency Prices Using Lstm, Svm And Polynomial Regression...
Prediction Of Cryptocurrency Prices Using Lstm, Svm And Polynomial Regression...ferisulianta.com
 
TCFPro24 Building Real-Time Generative AI Pipelines
TCFPro24 Building Real-Time Generative AI PipelinesTCFPro24 Building Real-Time Generative AI Pipelines
TCFPro24 Building Real-Time Generative AI PipelinesTimothy Spann
 
2024 Build Generative AI for Non-Profits
2024 Build Generative AI for Non-Profits2024 Build Generative AI for Non-Profits
2024 Build Generative AI for Non-ProfitsTimothy Spann
 
Stochastic Dynamic Programming and You.pptx
Stochastic Dynamic Programming and You.pptxStochastic Dynamic Programming and You.pptx
Stochastic Dynamic Programming and You.pptxjkmrshll88
 
Using DAX & Time-based Analysis in Data Warehouse
Using DAX & Time-based Analysis in Data WarehouseUsing DAX & Time-based Analysis in Data Warehouse
Using DAX & Time-based Analysis in Data WarehouseThinkInnovation
 
Paul Martin (Gartner) - Show Me the AI Money.pdf
Paul Martin (Gartner) - Show Me the AI Money.pdfPaul Martin (Gartner) - Show Me the AI Money.pdf
Paul Martin (Gartner) - Show Me the AI Money.pdfdcphostmaster
 

Último (20)

Unleashing Datas Potential - Mastering Precision with FCO-IM
Unleashing Datas Potential - Mastering Precision with FCO-IMUnleashing Datas Potential - Mastering Precision with FCO-IM
Unleashing Datas Potential - Mastering Precision with FCO-IM
 
STOCK PRICE ANALYSIS Furkan Ali TASCI --.pptx
STOCK PRICE ANALYSIS  Furkan Ali TASCI --.pptxSTOCK PRICE ANALYSIS  Furkan Ali TASCI --.pptx
STOCK PRICE ANALYSIS Furkan Ali TASCI --.pptx
 
Data Collection from Social Media Platforms
Data Collection from Social Media PlatformsData Collection from Social Media Platforms
Data Collection from Social Media Platforms
 
Air Con Energy Rating Info411 Presentation.pdf
Air Con Energy Rating Info411 Presentation.pdfAir Con Energy Rating Info411 Presentation.pdf
Air Con Energy Rating Info411 Presentation.pdf
 
Microeconomic Group Presentation Apple.pdf
Microeconomic Group Presentation Apple.pdfMicroeconomic Group Presentation Apple.pdf
Microeconomic Group Presentation Apple.pdf
 
Data Analytics Fundamentals: data analytics types.potx
Data Analytics Fundamentals: data analytics types.potxData Analytics Fundamentals: data analytics types.potx
Data Analytics Fundamentals: data analytics types.potx
 
STOCK PRICE ANALYSIS Furkan Ali TASCI --.pptx
STOCK PRICE ANALYSIS  Furkan Ali TASCI --.pptxSTOCK PRICE ANALYSIS  Furkan Ali TASCI --.pptx
STOCK PRICE ANALYSIS Furkan Ali TASCI --.pptx
 
Brain Tumor Detection with Machine Learning.pptx
Brain Tumor Detection with Machine Learning.pptxBrain Tumor Detection with Machine Learning.pptx
Brain Tumor Detection with Machine Learning.pptx
 
The market for cross-border mortgages in Europe
The market for cross-border mortgages in EuropeThe market for cross-border mortgages in Europe
The market for cross-border mortgages in Europe
 
Enabling GenAI Breakthroughs with Knowledge Graphs
Enabling GenAI Breakthroughs with Knowledge GraphsEnabling GenAI Breakthroughs with Knowledge Graphs
Enabling GenAI Breakthroughs with Knowledge Graphs
 
Neo4j Graph Summit 2024 Workshop - EMEA - Breda_and_Munchen.pdf
Neo4j Graph Summit 2024 Workshop - EMEA - Breda_and_Munchen.pdfNeo4j Graph Summit 2024 Workshop - EMEA - Breda_and_Munchen.pdf
Neo4j Graph Summit 2024 Workshop - EMEA - Breda_and_Munchen.pdf
 
Bengaluru Tableau UG event- 2nd March 2024 Q1
Bengaluru Tableau UG event- 2nd March 2024 Q1Bengaluru Tableau UG event- 2nd March 2024 Q1
Bengaluru Tableau UG event- 2nd March 2024 Q1
 
How to Build an Experimentation Culture for Data-Driven Product Development
How to Build an Experimentation Culture for Data-Driven Product DevelopmentHow to Build an Experimentation Culture for Data-Driven Product Development
How to Build an Experimentation Culture for Data-Driven Product Development
 
Neo4j_Jesus Barrasa_The Art of the Possible with Graph.pptx.pdf
Neo4j_Jesus Barrasa_The Art of the Possible with Graph.pptx.pdfNeo4j_Jesus Barrasa_The Art of the Possible with Graph.pptx.pdf
Neo4j_Jesus Barrasa_The Art of the Possible with Graph.pptx.pdf
 
Prediction Of Cryptocurrency Prices Using Lstm, Svm And Polynomial Regression...
Prediction Of Cryptocurrency Prices Using Lstm, Svm And Polynomial Regression...Prediction Of Cryptocurrency Prices Using Lstm, Svm And Polynomial Regression...
Prediction Of Cryptocurrency Prices Using Lstm, Svm And Polynomial Regression...
 
TCFPro24 Building Real-Time Generative AI Pipelines
TCFPro24 Building Real-Time Generative AI PipelinesTCFPro24 Building Real-Time Generative AI Pipelines
TCFPro24 Building Real-Time Generative AI Pipelines
 
2024 Build Generative AI for Non-Profits
2024 Build Generative AI for Non-Profits2024 Build Generative AI for Non-Profits
2024 Build Generative AI for Non-Profits
 
Stochastic Dynamic Programming and You.pptx
Stochastic Dynamic Programming and You.pptxStochastic Dynamic Programming and You.pptx
Stochastic Dynamic Programming and You.pptx
 
Using DAX & Time-based Analysis in Data Warehouse
Using DAX & Time-based Analysis in Data WarehouseUsing DAX & Time-based Analysis in Data Warehouse
Using DAX & Time-based Analysis in Data Warehouse
 
Paul Martin (Gartner) - Show Me the AI Money.pdf
Paul Martin (Gartner) - Show Me the AI Money.pdfPaul Martin (Gartner) - Show Me the AI Money.pdf
Paul Martin (Gartner) - Show Me the AI Money.pdf
 

Phage presentation spring 2014

  • 1. ISOLATION OF A NOVEL MYCOBACTERIOPHAGE Lizbeth Pérez Castro Javier Zavala Ayala Mónica Del Moral Félix Valles
  • 2. WHAT IS A BACTERIOPHAGE? • A bacteriophage is any virus that infects any bacterium. • Mycobacteriophages are viruses that infect mycobacteria. • All mycobacteriophages are bacteriophages, but not all bacteriophages are mycobacteriophages. • Found in different bacterial colonies. • Mostly found in the soil and sea water. • Resistant to antibiotics.
  • 3. BACTERIAS FOR HOSTING 1. M. smegmatis (Smeg) • fast growing rate and non-pathogenic behaviour • Aerobic organisms • Found in water, soil and plants 2. Bacillus • Obligate aerobes or facultative anaerobes • Well understood- used as a model for differentiation, gene regulation, and cell cycle events in bacteria. • Common in soil  Both are:  Gram positive  Acid fast bacteria  Morfology- rodshape
  • 4. BACTERIOPHAGE STRUCTURE • Phages consist of 3 basic components: • Capsid-contains the genetic material • Genetic material- DNA • Tail-serves to attach to bacteria and DNA passes through tail and into bacterium http://en.wikipedia.org/wiki/Bacteriophage
  • 5. PHAGE LIFE CYCLES • Temperate-Lisogenic: • Replicate and lyse or dormant state- incorporate its genetic material into DNA of host. • Virulent-Lytic: • Straightforward cycle by lysing all bacteria they infect.
  • 8. MY BACTERIOPHAGE • Name: Nitidusvenutus • Located in: Carretera 943 Sector Los Chinos, Gurabo • Coordinates: 18º 16’ 7’ N, 65º 58’ 32’ W • Collected: February 24, 2014 • It was found in a rural area next to cow feces.
  • 12. MY BACTERIOPHAGE: MUSAMODEL • Name- Musamodel • Located in: 1-9 calle Barcelona, Urb.Terralinda, Caguas P.R • Coordinates: 88˚14’6”N , 66˚2’39”W • Collected: March 16, 2014 • Time: 7:51pm • Temperature: 79˚ • About 3 inches deep and 1 foot close to concrete • Found next to a plantain tree • Soil was moist and loose
  • 14. SAMPLES COLLECTED 1st 2nd 3rd 4th 5th 6th 7th Date 2/6/14 2/18/14 2/24/14 3/3/14 3/10/14 3/16/14 3/16/14 Time 7:53 am 7:06 am 9:55 pm 9:38 am 6:26 am 7:45 pm 7:51 pm Coordinates 18˚14’6”N, 66˚2’40”W 18˚14’13”N, 66˚2’35”W 18˚14’7”N, 66˚2’39”W 18˚14’18”N, 66˚2’42”W 18˚14’7”N, 66˚2’40”W 18˚14’6”N, 66˚2’39”W 18˚14’17”N, 66˚2’42”W Temperature 75˚ 75˚ 79˚ 81˚ 73˚ 79˚ 79˚ Soil description Moist, 3 inches deep Moist, 1 inch deep Moist, 1 inch deep Moist, with moss, 1 inch deep Moist, 1 inch Moist, loose, 5 inches deep Moist, loose, 3 inches deep Site description Urban, 5 feet close to concrete Urban, 1 foot from concrete Urban, 2 feet from concrete Urban, ½ foot from concrete Urban, 3 feet away from concrete Urban, 1 foot close to concrete Next to a plantain plant, 1 foot close to concrete
  • 16. CONCLUSION • Two mycobacteriophages were found: 1. Nitidusvenutus a. located in Carretera 943 Sector Los Chinos, Gurabo b. ……. Were completed 2. Musamodel a. located in 1-9 calle Barcelona, Urb. Terralinda, Caguas P.R b. Up to now, we believe it has a lysogenic life cycle because of turbidness c. three purifications were completed
  • 17. FUTURE PLANS • Nitidusvenutus • Musamodel; • 3rd purification- to have more isolated plaques and measure diameter • Spot test • Make phage stocks- • Empirical test • 10 plate preparation • Analyze • Bioinformatics and sequencing
  • 18. ACKNOWLEDGEMENT • Dr. Michael Rubin • Juan Apiz • Eduardo Correa • Gustavo Martínez • Giovanni Cruz