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Biotechnology Process and Application 
Exercises 
Q.1 Donor DNA is introduced into host cell by a process called------------- 
(a) lysis 
(b) transcription 
(c) transformation 
(d) translation. 
Q.2 A gene introduced into a plant is-------------- 
(a) jumping gene. 
(b) transgene. 
(c) recessive gene. 
(d) None of these. 
Q.3 One of the following has become important in plant breeding------------- 
(a) plasmid - E coli. 
(b) cosmid – phages. 
(c) Ti plasmid – Agrobacterium 
(d) both (a) and (b) 
Q.4 Cry protein is synthesized by------------------- 
(a) BT toxin gene. 
(b) GM gene of yeast. 
(c) Ti plasmid. 
(d) cosmid. 
Q.5 Endonucleases differ from restriction endonucleases in---------------- 
(a) They cut DNA at any point. 
(b) They cut DNA at specific point. 
(c) They cut DNA at any point except the ends. 
(d) None of these.
Q.6 NiF gene is present in -------- for---------- fixation. 
(a) Azotobactor – Nitrogen. 
(b) cyanobactrium – oxygen. 
(c) Rhizobium - CO2. 
(d) Rhizobium - N2. 
Q.7 Ripening of tomato is delayed by transferring a gene which produces enzyme---- 
(a) Cellulose. 
(b) Polygalactournase. 
(c) Pectin. 
(d) Hydralases. 
Q.8 The number of genomes in a diploid cell is--------------- 
(a) One. 
(b) Two. 
(c) Four. 
(d) Any number. 
Q.9 In a gene library, the DNA sequences of an organism are stored & maintained as----- 
(a) Entire DNA. 
(b) Free DNA fragments of correct size. 
(c) m-RNA. 
(d) DNA fragments cloned into vectors. 
Q.10 Gene library can be maintained ---------- 
(a) At room temperature for one year. 
(b) At -150º C in liquid N2 for 5 years. 
(c) In cloning vectors for 1 week. 
(d) In cloning vectors in culture media indefinitely. 
Answer Key: 
1-c 2-b 3-c 4-a 5-c 
6-d 7-b 8-b 9-d 10-d
Keep on visiting www.ednexa.com for more study material about MH-CET 2014. 
- Team Ednexa

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Biotechnology Process and Application Exercise - MH-CET 2015

  • 1. Biotechnology Process and Application Exercises Q.1 Donor DNA is introduced into host cell by a process called------------- (a) lysis (b) transcription (c) transformation (d) translation. Q.2 A gene introduced into a plant is-------------- (a) jumping gene. (b) transgene. (c) recessive gene. (d) None of these. Q.3 One of the following has become important in plant breeding------------- (a) plasmid - E coli. (b) cosmid – phages. (c) Ti plasmid – Agrobacterium (d) both (a) and (b) Q.4 Cry protein is synthesized by------------------- (a) BT toxin gene. (b) GM gene of yeast. (c) Ti plasmid. (d) cosmid. Q.5 Endonucleases differ from restriction endonucleases in---------------- (a) They cut DNA at any point. (b) They cut DNA at specific point. (c) They cut DNA at any point except the ends. (d) None of these.
  • 2. Q.6 NiF gene is present in -------- for---------- fixation. (a) Azotobactor – Nitrogen. (b) cyanobactrium – oxygen. (c) Rhizobium - CO2. (d) Rhizobium - N2. Q.7 Ripening of tomato is delayed by transferring a gene which produces enzyme---- (a) Cellulose. (b) Polygalactournase. (c) Pectin. (d) Hydralases. Q.8 The number of genomes in a diploid cell is--------------- (a) One. (b) Two. (c) Four. (d) Any number. Q.9 In a gene library, the DNA sequences of an organism are stored & maintained as----- (a) Entire DNA. (b) Free DNA fragments of correct size. (c) m-RNA. (d) DNA fragments cloned into vectors. Q.10 Gene library can be maintained ---------- (a) At room temperature for one year. (b) At -150º C in liquid N2 for 5 years. (c) In cloning vectors for 1 week. (d) In cloning vectors in culture media indefinitely. Answer Key: 1-c 2-b 3-c 4-a 5-c 6-d 7-b 8-b 9-d 10-d
  • 3. Keep on visiting www.ednexa.com for more study material about MH-CET 2014. - Team Ednexa