The Most Advanced Digital Pathology Image Analysis Solution

1. Definiens Tissue Studio™ The Most Advanced Digital Pathology Image Analysis Solution

2. Definiens Tissue Studio™ overview Enables simple, fast, and accurate ROI detection via the new Definiens Composer Technology™ Provides multi-parametric quantitationon a cell-by-cell basis Handles heterogeneous tissue samples Works with whole slides and TMAs Handles images acquired from any source Operates as a dedicated workstation or as a client server application

3. Definiens Composer Technology™ What is it? Definiens Composer Technology™ was developed to enable the user to easily teach the system how to identify regions of interest (ROIs). How does it work? Simply load up to 4 representative images, and use the “paintbrush tool” on a few representative ROIs in your images. Then click the “Learn” button. How does this differ from other approaches? Training the system with 4 representative images ensures a robust image analysis solution without over fitting. Combines machine learning, pattern recognition and auto adaptive analysis algorithms based on Definiens eCognitionNetwork Language ™. Definiens Tissue Studio™ is faster and more flexible.

4. ROI Detection and Cell by Cell Analysis across and within selected ROIs Definiens Tissue Studio™ Workflow

5. Load training images

6. Define image analysis solution

7. Load up to four different images:Detect tissue / Background / Segment images

8. Definiens Composer Technology™:“Paint” on a few representative ROIs

9. Definiens Composer Technology™:After painting a few ROIs, click the “Learn” button

10. Definiens Composer Technology™:Computer has now been “trained”

11. Definiens Composer Technology™:Merge “Object Primitives”

12. Second Definiens Composer step:Re-classify Islets into “tumor” and “normal”

13. Select ROIs in tumor islets

14. ROIs at 20xParameters can be adjusted and previewed here

15. Detect nuclei

16. Batch Process:Analyze selected images

17. Export multi-parametric quantitative features

18. List of supported instruments Hamamatsu: Nanozoomer Zeiss: Mirax Aperio: Scanscope Applied Imaging: Ariol TissueGnostics Bacus WebSlide DMetrix Generic (tif, jpg, etc.)

19. Summary Deeper insights Quantifies nuclear, membrane and cytoplasmic biomarkers on a cell-by-cell basis Supports all standard IHC tissue stains (blue, brown and red), H&E and IF Analyzes all slides and TMA images from all major image acquisition devices Faster results Identifies regions of interest automatically Operates through a simple, intuitive user interface Offers unlimited throughput with parallel batch processing Better decisions Delivers accurate and reproducible results Enables multiparametric investigations to identify underlying correlations Supports translational research through rapid iterative investigation of biomarkers

20. Questions Contact: Daniel R. Nicolson Director, Global Accounts 484-678-9090 dnicolson@definiens.com