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The
Protozoans
Common Protozoa of Man
Introduction
• The distinction between
  plant-   like  &   animal-
  like  organisms    is  not
  always     easily     made,
  particularly         among
  unicellular life forms.
  This difficulty reflects
  what is surely a distant
  but       very        real
  evolutionary
  relationship      between
  plants & animals.
• Protozoans are clearly
• Protozoan classification has
  long    been       uncertain,
  however,          nucleotide
  sequence data in particular
  are changing the scenarios
  of                 protozoan
  interrelationships at a rapid
  rate.
• Controversy abounds at the
  most   fundamental    levels
  of      organization.      Of
  particular          interest,
  hundreds    of   unicellular
  species  lack   mitochondria.
  Whether     these     species
General Characteris tics
• It  is  difficult   to
  generalize      about
  protozoans;       they
  absolutely defy tidy
  categorization.
• Structural
  Description    of     A
  Protozoa:
           The    entire
  protozoan    body    is
  bounded      by       a
• The cytoplasm bounded
  by     the      p l a s ma l e mma
  resembles        that           of
  animal    cells,        except
  that     it     is        often
  differentiated         into      a
  clear, gelatinous outer
  region, the e c t o p l a s m, &
  an   inner,   more          fluid
  region, the e nd o p l a s m.
• Contractile         vacuoles
  are organelles involved
  in expelling water from
Phylum
Ciliophora
Defining Characteris tics
• Body            externally
  ciliated   in    at   least
  some life cycle stages.
• Individual      cilia   are
  connected      below    the
  body surface through a
  complex cord of fibers
  (the infraciliature).
• Ciliates      show      the
  highest      degree      of
  subcellular
  specialization
  encountered           among
• First    among     the
  features     of    this
  phylum      is     the
  presence     of    the
  external ciliation in
  at least some stage
  of the life cycle.
• The   ultrastructure
  of       cilia       is
  remarkably     uniform
  throughout         the
S tructure & F unction of Cilia
• Each          cilium            is
  cylindrical & arises
  from        a   b a sa l    b o dy
  (kinetosome).             Within
  the       cilium       are       a
  number of long rods
  called         mic r o t ub ul e s ,
  composed              of         a
  protein        known           as
  t ub ul in.
• A       cross          section
• One pair of microtubule
  is     located           centrally
  within the cilium. These
  two microtubules form
  the central shaft the
  cilium,        and       they  are
  often surrounded by a
  membrane constituting a
  c e nt r a l s he a t h.
• The entire microtubular
  complex,          consisting    of
  the           nine         doublet
  microtubules               &   the
Pattern of Ciliation
• Individual           cilia,          seen
  external           to      the       cell
  body, are associated w/
  each      other         through           a
  complex               inf r a c il ia t ur e
  below the body surface.
• A striated fibril, called
  a   k ine t o d e s mo s ,   extends
  from       each         kinetosome
  (basal         body)         in        the
  direction of an adjacent
  cilium of the same row.
• Thus, running along the
• In some species, groups
  of              cilia         are
  functionally associated
  in such a way to form
  discrete            organelles.
  One     such      organelle     is
  the so-called und ul a t ing
  me mb r a ne ,    a   flattened
  sheet          of   cilia   that
  moves as a single unit.
• A      second          commonly
  encountered               ciliary
  organelle is termed a
Other Morpholog ical F eatures
• One                    other
  morphological
  feature particularly
  characteristic              of
  ciliates      is           the
  covering of the body
  by an often complex
  series of membranes,
  forming a p e l l ic l e .
• The inner membranes,
  lying    beneath           the
• Trichocysts        are
  characteristically
  associated   with  the
  ciliate pellicle.
• Some    species   also
  maintain a permanent
  opening     to     the
  outside (a cytproct;
  procto = G: the anus)
  for          expelling
  undigested wastes.
Reproductive Characteris tics
• In       addition              to   the
  presence of cilia & an
  infraciliature, a second
  unique characteristic of
  all        ciliates             is  the
  possession                  by     each
  individual of two types
  of nuclei; that is, the
  nuclei of every ciliate
  are       d imo r p hic       (of   two
  kinds).
• The      ciliates            are   thus
  he t e r o k a r y o t ic ,        while
Ciliate Lifes tyles
• About   65%   of   ciliate
  species are free-living,
  &  most   of  these    are
  motile.    Some     other
  species form temporary
  attachments     to   living
  or nonliving substrates
  for   feeding   purposes,
  while     others       are
  permanently attached &
  many may form colonies.
• Although    all   ciliates
  have a distinct pellicle,

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Protozoa I

  • 3. Introduction • The distinction between plant- like & animal- like organisms is not always easily made, particularly among unicellular life forms. This difficulty reflects what is surely a distant but very real evolutionary relationship between plants & animals. • Protozoans are clearly
  • 4. • Protozoan classification has long been uncertain, however, nucleotide sequence data in particular are changing the scenarios of protozoan interrelationships at a rapid rate. • Controversy abounds at the most fundamental levels of organization. Of particular interest, hundreds of unicellular species lack mitochondria. Whether these species
  • 5. General Characteris tics • It is difficult to generalize about protozoans; they absolutely defy tidy categorization. • Structural Description of A Protozoa: The entire protozoan body is bounded by a
  • 6. • The cytoplasm bounded by the p l a s ma l e mma resembles that of animal cells, except that it is often differentiated into a clear, gelatinous outer region, the e c t o p l a s m, & an inner, more fluid region, the e nd o p l a s m. • Contractile vacuoles are organelles involved in expelling water from
  • 8. Defining Characteris tics • Body externally ciliated in at least some life cycle stages. • Individual cilia are connected below the body surface through a complex cord of fibers (the infraciliature). • Ciliates show the highest degree of subcellular specialization encountered among
  • 9. • First among the features of this phylum is the presence of the external ciliation in at least some stage of the life cycle. • The ultrastructure of cilia is remarkably uniform throughout the
  • 10. S tructure & F unction of Cilia • Each cilium is cylindrical & arises from a b a sa l b o dy (kinetosome). Within the cilium are a number of long rods called mic r o t ub ul e s , composed of a protein known as t ub ul in. • A cross section
  • 11. • One pair of microtubule is located centrally within the cilium. These two microtubules form the central shaft the cilium, and they are often surrounded by a membrane constituting a c e nt r a l s he a t h. • The entire microtubular complex, consisting of the nine doublet microtubules & the
  • 12. Pattern of Ciliation • Individual cilia, seen external to the cell body, are associated w/ each other through a complex inf r a c il ia t ur e below the body surface. • A striated fibril, called a k ine t o d e s mo s , extends from each kinetosome (basal body) in the direction of an adjacent cilium of the same row. • Thus, running along the
  • 13. • In some species, groups of cilia are functionally associated in such a way to form discrete organelles. One such organelle is the so-called und ul a t ing me mb r a ne , a flattened sheet of cilia that moves as a single unit. • A second commonly encountered ciliary organelle is termed a
  • 14. Other Morpholog ical F eatures • One other morphological feature particularly characteristic of ciliates is the covering of the body by an often complex series of membranes, forming a p e l l ic l e . • The inner membranes, lying beneath the
  • 15. • Trichocysts are characteristically associated with the ciliate pellicle. • Some species also maintain a permanent opening to the outside (a cytproct; procto = G: the anus) for expelling undigested wastes.
  • 16.
  • 17. Reproductive Characteris tics • In addition to the presence of cilia & an infraciliature, a second unique characteristic of all ciliates is the possession by each individual of two types of nuclei; that is, the nuclei of every ciliate are d imo r p hic (of two kinds). • The ciliates are thus he t e r o k a r y o t ic , while
  • 18.
  • 19.
  • 20.
  • 21.
  • 22. Ciliate Lifes tyles • About 65% of ciliate species are free-living, & most of these are motile. Some other species form temporary attachments to living or nonliving substrates for feeding purposes, while others are permanently attached & many may form colonies. • Although all ciliates have a distinct pellicle,