SlideShare ist ein Scribd-Unternehmen logo
1 von 27
Downloaden Sie, um offline zu lesen
DANIEL SMITH
ARGONNE NATIONAL LABORATORY
YOUR MICROBIOTA


 10bacterial cells for
 every human cell.

 Human  health is
 dependent on
 microbiota
YOUR MICROBIOTA
 Science,   2009
YOUR MICROBIOTA
 Nature   Reviews Microbiology, 2011
YOUR HOME’S MICROBIOME
YOUR HOME’S MICROBIOME
 Building-specific  seasonal patterns in
  bacteria found on dust.
 In-progress research is exploring the
  microbial communities of several locations
  in houses: kitchen surfaces (including inside
  the refrigerator), tv screens, pillows, the
  water inside of water heaters, etc, etc.
INTERACTION OF MICROBIOMES
 Human    shed about 1.5 millions cells per
  hour, along with their associated 15
  million bacteria cells.
 Direct contact can transfer millions of
  cells in each event.
INTERACTION OF MICROBIOMES
 There is a lack of evidence regarding how
 rapidly the human skin microbiota is
 transferred to the surfaces of the homes in
 which we live.

 Hypotheses:
     Microbes from the occupant are transferred rapidly
      to the home and replace existing communities.
     The occupant’s microbiome is reflected by the
      home’s microbiome.
     Bacteria on surfaces will be most similar to the
      bacteria on the body parts that come into contact
      with it.
WHEN BIOMES COLLIDE



              ?
  +          =
FIELD STUDY
 Monitoring the home and human microbiota
 of relocating “citizen scientists”.
SAMPLING
   Human:                House:
       Palm of hand          Kitchen countertop
       Heel of foot          Kitchen floor
       Inside of nose        Kitchen light switch
                              Bedroom floor
                              Front door knob
                              Bathroom door knob
HOME METADATA
 Type  of paint on walls
 Countertop material

 Type of flooring

 Light intensity

 HVAC system

 Pets in household

 Square footage
DAILY METADATA
 Placedfive-minute interval data loggers in
 the bathroom, kitchen, and bedroom to
 track:
     Relative Humidity
     Temperature
     Dew Point
 Participants  also
 fill out the form at
 right each time
 they collect
 samples.
DATA LOGGER DATA
           Temperature        Rel. Humidity           Dew Point

   36 °C                                      76 RH
                  Bathroom
   28 °C                                      65 RH
   20 °C                                      54 RH
   12 °C                                      43 RH
    4 °C                                      32 RH

             Bedroom
20 °C                                                 47 RH

12 °C                                                 38 RH

 4 °C                                                 28 RH

                    Kitchen
        20 °C                                            40 RH
        12 °C                                            32 RH
           4 °C                                          24 RH
ANALYSIS
  PCR-Amplify 16S rDNA V4-V5 Region
                  ↓
    Sequence 150bp x2 (paired end)
                  ↓
    QIIME Phylogenetic Assignment
                  ↓
     Principle Coordinate Analysis
ALPHA DIVERSITY
ALPHA DIVERSITY
 Most  Diverse: Nose, hand, and hand-
  contact surfaces in house #1.
 Most Diverse House: House 1, followed by
  2, then 3.
    Front_Door_Knob
      Bathroom_Floor
        Kitchen_Floor
 Kitchen_Light_Switch
 Bathroom_Door_Knob
                Hand
       Bedroom_Floor
                Nose
     Kitchen_Counter
                Foot

                        0   20    40   60   80    100 120 140   160
                                 Observed Species (average)
HOUSE 1 RESULTS
                                                                             Staphylococcaceae
                              Hand       Nose        Foot
                      50%                                                    Mycoplasmataceae
                                                                      50%
% Total Population




                                                                             Moraxellaceae (A)
                      40%                                             40%
                                                                      30%    Corynebacteriaceae
                      30%                                                    Streptophyta
                                                                      20%
                      20%                                             10%    Moraxellaceae (B)
                      10%                                              0%    Streptococcaceae
                                                                             Actinomycetales
                                                                             1 5 9 13
                       0%
                                                                             Pseudomonadaceae

                            Bedroom   Kitchen   Kitchen     Kitchen         Bathroom    Front Door
                             Floor     Floor    Counter      Light          Door Knob     Knob
 % Total Population




                      80%                                   Switch

                      60%

                      40%

                      20%

                      0%
                                                          Tim
                                                           e
HOUSE 2 RESULTS
                 Person 1                         Person 2
       Hand       Nose       Foot       Hand       Nose        Foot
100%
80%
60%
40%
20%
 0%


       Bedroom     Kitchen   Kitchen    Kitchen   Bathroom    Front Door
        Floor       Floor    Counter     Light    Door Knob     Knob
60%
                                        Switch
50%
40%
30%
20%
10%
 0%


       Staphylococcaceae
               50%            Mycoplasmataceae      Moraxellaceae (A)
       Corynebacteriaceae     Streptophyta          Moraxellaceae (B)
       Streptococcaceae
                0%            Actinomycetales       Pseudomonadaceae
HOUSE 3 RESULTS
              Person 1                 Person 2                 Person 3
       Hand    Nose      Foot   Hand    Nose      Foot   Hand    Nose      Foot
100%
80%
60%
40%
20%
 0%


       Bedroom        Kitchen    Kitchen       Kitchen   Bathroom Front Door
        Floor          Floor     Counter        Light    Door Knob  Knob
 80%
                                               Switch
 60%

 40%

 20%

  0%


        Staphylococcaceae
                50%              Mycoplasmataceae         Moraxellaceae (A)
        Corynebacteriaceae       Streptophyta             Moraxellaceae (B)
        Streptococcaceae
                 0%              Actinomycetales          Pseudomonadaceae
3D PRINCIPLE COORDINATE
PLOTS
HAND MICROBIAL COMMUNITIES

Individuals from
different houses   Individuals from the same
                   house
BEDROOM FLOORS
KITCHEN COUNTERS
KITCHEN FLOORS
LIVE UPDATES
 HomeMicrobiome.com

 Facebook.com/HomeMicrobiome

 Twitter.com/HomeMicrobiome


 Alldata publicly
  available
ACKNOWLEDGEMENTS
 Jack Gilbert – P.I.
 Jarrad Hampton-Marcell – Sample Preps

 Sarah Owens – Sequencing Manager

 Alfred P. Sloan Foundation - Funding

Weitere ähnliche Inhalte

Kürzlich hochgeladen

Role of Soap based and synthetic or syndets bar
Role of  Soap based and synthetic or syndets barRole of  Soap based and synthetic or syndets bar
Role of Soap based and synthetic or syndets barmohitRahangdale
 
BENIGN BREAST DISEASE
BENIGN BREAST DISEASE BENIGN BREAST DISEASE
BENIGN BREAST DISEASE Mamatha Lakka
 
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA .pdf
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA    .pdfSGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA    .pdf
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA .pdfHongBiThi1
 
power point presentation of Clinical evaluation of strabismus
power point presentation of Clinical evaluation  of strabismuspower point presentation of Clinical evaluation  of strabismus
power point presentation of Clinical evaluation of strabismusChandrasekar Reddy
 
CONNECTIVE TISSUE (ANATOMY AND PHYSIOLOGY).pdf
CONNECTIVE TISSUE (ANATOMY AND PHYSIOLOGY).pdfCONNECTIVE TISSUE (ANATOMY AND PHYSIOLOGY).pdf
CONNECTIVE TISSUE (ANATOMY AND PHYSIOLOGY).pdfDolisha Warbi
 
ORAL HYPOGLYCAEMIC AGENTS - PART 2.pptx
ORAL HYPOGLYCAEMIC AGENTS  - PART 2.pptxORAL HYPOGLYCAEMIC AGENTS  - PART 2.pptx
ORAL HYPOGLYCAEMIC AGENTS - PART 2.pptxNIKITA BHUTE
 
FDMA FLAP - The first dorsal metacarpal artery (FDMA) flap is used mainly for...
FDMA FLAP - The first dorsal metacarpal artery (FDMA) flap is used mainly for...FDMA FLAP - The first dorsal metacarpal artery (FDMA) flap is used mainly for...
FDMA FLAP - The first dorsal metacarpal artery (FDMA) flap is used mainly for...Shubhanshu Gaurav
 
MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.whalesdesign
 
Mental health Team. Dr Senthil Thirusangu
Mental health Team. Dr Senthil ThirusanguMental health Team. Dr Senthil Thirusangu
Mental health Team. Dr Senthil Thirusangu Medical University
 
ANATOMICAL FAETURES OF BONES FOR NURSING STUDENTS .pptx
ANATOMICAL FAETURES OF BONES  FOR NURSING STUDENTS .pptxANATOMICAL FAETURES OF BONES  FOR NURSING STUDENTS .pptx
ANATOMICAL FAETURES OF BONES FOR NURSING STUDENTS .pptxWINCY THIRUMURUGAN
 
Using Data Visualization in Public Health Communications
Using Data Visualization in Public Health CommunicationsUsing Data Visualization in Public Health Communications
Using Data Visualization in Public Health Communicationskatiequigley33
 
blood bank management system project report
blood bank management system project reportblood bank management system project report
blood bank management system project reportNARMADAPETROLEUMGAS
 
Different drug regularity bodies in different countries.
Different drug regularity bodies in different countries.Different drug regularity bodies in different countries.
Different drug regularity bodies in different countries.kishan singh tomar
 
SGK ĐIỆN GIẬT ĐHYHN RẤT LÀ HAY TUYỆT VỜI.pdf
SGK ĐIỆN GIẬT ĐHYHN        RẤT LÀ HAY TUYỆT VỜI.pdfSGK ĐIỆN GIẬT ĐHYHN        RẤT LÀ HAY TUYỆT VỜI.pdf
SGK ĐIỆN GIẬT ĐHYHN RẤT LÀ HAY TUYỆT VỜI.pdfHongBiThi1
 
Trustworthiness of AI based predictions Aachen 2024
Trustworthiness of AI based predictions Aachen 2024Trustworthiness of AI based predictions Aachen 2024
Trustworthiness of AI based predictions Aachen 2024EwoutSteyerberg1
 
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptx
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptxBreast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptx
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptxNaveenkumar267201
 
"Radical excision of DIE in subferile women with deep infiltrating endometrio...
"Radical excision of DIE in subferile women with deep infiltrating endometrio..."Radical excision of DIE in subferile women with deep infiltrating endometrio...
"Radical excision of DIE in subferile women with deep infiltrating endometrio...Sujoy Dasgupta
 
EXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung functionEXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung functionkrishnareddy157915
 

Kürzlich hochgeladen (20)

Role of Soap based and synthetic or syndets bar
Role of  Soap based and synthetic or syndets barRole of  Soap based and synthetic or syndets bar
Role of Soap based and synthetic or syndets bar
 
BENIGN BREAST DISEASE
BENIGN BREAST DISEASE BENIGN BREAST DISEASE
BENIGN BREAST DISEASE
 
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA .pdf
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA    .pdfSGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA    .pdf
SGK NGẠT NƯỚC ĐHYHN RẤT LÀ HAY NHA .pdf
 
power point presentation of Clinical evaluation of strabismus
power point presentation of Clinical evaluation  of strabismuspower point presentation of Clinical evaluation  of strabismus
power point presentation of Clinical evaluation of strabismus
 
CONNECTIVE TISSUE (ANATOMY AND PHYSIOLOGY).pdf
CONNECTIVE TISSUE (ANATOMY AND PHYSIOLOGY).pdfCONNECTIVE TISSUE (ANATOMY AND PHYSIOLOGY).pdf
CONNECTIVE TISSUE (ANATOMY AND PHYSIOLOGY).pdf
 
Biologic therapy ice breaking in rheumatology, Case based approach with appli...
Biologic therapy ice breaking in rheumatology, Case based approach with appli...Biologic therapy ice breaking in rheumatology, Case based approach with appli...
Biologic therapy ice breaking in rheumatology, Case based approach with appli...
 
ORAL HYPOGLYCAEMIC AGENTS - PART 2.pptx
ORAL HYPOGLYCAEMIC AGENTS  - PART 2.pptxORAL HYPOGLYCAEMIC AGENTS  - PART 2.pptx
ORAL HYPOGLYCAEMIC AGENTS - PART 2.pptx
 
How to master Steroid (glucocorticoids) prescription, different scenarios, ca...
How to master Steroid (glucocorticoids) prescription, different scenarios, ca...How to master Steroid (glucocorticoids) prescription, different scenarios, ca...
How to master Steroid (glucocorticoids) prescription, different scenarios, ca...
 
FDMA FLAP - The first dorsal metacarpal artery (FDMA) flap is used mainly for...
FDMA FLAP - The first dorsal metacarpal artery (FDMA) flap is used mainly for...FDMA FLAP - The first dorsal metacarpal artery (FDMA) flap is used mainly for...
FDMA FLAP - The first dorsal metacarpal artery (FDMA) flap is used mainly for...
 
MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.
 
Mental health Team. Dr Senthil Thirusangu
Mental health Team. Dr Senthil ThirusanguMental health Team. Dr Senthil Thirusangu
Mental health Team. Dr Senthil Thirusangu
 
ANATOMICAL FAETURES OF BONES FOR NURSING STUDENTS .pptx
ANATOMICAL FAETURES OF BONES  FOR NURSING STUDENTS .pptxANATOMICAL FAETURES OF BONES  FOR NURSING STUDENTS .pptx
ANATOMICAL FAETURES OF BONES FOR NURSING STUDENTS .pptx
 
Using Data Visualization in Public Health Communications
Using Data Visualization in Public Health CommunicationsUsing Data Visualization in Public Health Communications
Using Data Visualization in Public Health Communications
 
blood bank management system project report
blood bank management system project reportblood bank management system project report
blood bank management system project report
 
Different drug regularity bodies in different countries.
Different drug regularity bodies in different countries.Different drug regularity bodies in different countries.
Different drug regularity bodies in different countries.
 
SGK ĐIỆN GIẬT ĐHYHN RẤT LÀ HAY TUYỆT VỜI.pdf
SGK ĐIỆN GIẬT ĐHYHN        RẤT LÀ HAY TUYỆT VỜI.pdfSGK ĐIỆN GIẬT ĐHYHN        RẤT LÀ HAY TUYỆT VỜI.pdf
SGK ĐIỆN GIẬT ĐHYHN RẤT LÀ HAY TUYỆT VỜI.pdf
 
Trustworthiness of AI based predictions Aachen 2024
Trustworthiness of AI based predictions Aachen 2024Trustworthiness of AI based predictions Aachen 2024
Trustworthiness of AI based predictions Aachen 2024
 
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptx
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptxBreast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptx
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptx
 
"Radical excision of DIE in subferile women with deep infiltrating endometrio...
"Radical excision of DIE in subferile women with deep infiltrating endometrio..."Radical excision of DIE in subferile women with deep infiltrating endometrio...
"Radical excision of DIE in subferile women with deep infiltrating endometrio...
 
EXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung functionEXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung function
 

2012 03-07 home microbiome - gsc13 china

  • 2. YOUR MICROBIOTA  10bacterial cells for every human cell.  Human health is dependent on microbiota
  • 4. YOUR MICROBIOTA  Nature Reviews Microbiology, 2011
  • 6. YOUR HOME’S MICROBIOME  Building-specific seasonal patterns in bacteria found on dust.  In-progress research is exploring the microbial communities of several locations in houses: kitchen surfaces (including inside the refrigerator), tv screens, pillows, the water inside of water heaters, etc, etc.
  • 7. INTERACTION OF MICROBIOMES  Human shed about 1.5 millions cells per hour, along with their associated 15 million bacteria cells.  Direct contact can transfer millions of cells in each event.
  • 8. INTERACTION OF MICROBIOMES  There is a lack of evidence regarding how rapidly the human skin microbiota is transferred to the surfaces of the homes in which we live.  Hypotheses:  Microbes from the occupant are transferred rapidly to the home and replace existing communities.  The occupant’s microbiome is reflected by the home’s microbiome.  Bacteria on surfaces will be most similar to the bacteria on the body parts that come into contact with it.
  • 10. FIELD STUDY  Monitoring the home and human microbiota of relocating “citizen scientists”.
  • 11. SAMPLING  Human:  House:  Palm of hand  Kitchen countertop  Heel of foot  Kitchen floor  Inside of nose  Kitchen light switch  Bedroom floor  Front door knob  Bathroom door knob
  • 12. HOME METADATA  Type of paint on walls  Countertop material  Type of flooring  Light intensity  HVAC system  Pets in household  Square footage
  • 13. DAILY METADATA  Placedfive-minute interval data loggers in the bathroom, kitchen, and bedroom to track:  Relative Humidity  Temperature  Dew Point  Participants also fill out the form at right each time they collect samples.
  • 14. DATA LOGGER DATA Temperature Rel. Humidity Dew Point 36 °C 76 RH Bathroom 28 °C 65 RH 20 °C 54 RH 12 °C 43 RH 4 °C 32 RH Bedroom 20 °C 47 RH 12 °C 38 RH 4 °C 28 RH Kitchen 20 °C 40 RH 12 °C 32 RH 4 °C 24 RH
  • 15. ANALYSIS PCR-Amplify 16S rDNA V4-V5 Region ↓ Sequence 150bp x2 (paired end) ↓ QIIME Phylogenetic Assignment ↓ Principle Coordinate Analysis
  • 17. ALPHA DIVERSITY  Most Diverse: Nose, hand, and hand- contact surfaces in house #1.  Most Diverse House: House 1, followed by 2, then 3. Front_Door_Knob Bathroom_Floor Kitchen_Floor Kitchen_Light_Switch Bathroom_Door_Knob Hand Bedroom_Floor Nose Kitchen_Counter Foot 0 20 40 60 80 100 120 140 160 Observed Species (average)
  • 18. HOUSE 1 RESULTS Staphylococcaceae Hand Nose Foot 50% Mycoplasmataceae 50% % Total Population Moraxellaceae (A) 40% 40% 30% Corynebacteriaceae 30% Streptophyta 20% 20% 10% Moraxellaceae (B) 10% 0% Streptococcaceae Actinomycetales 1 5 9 13 0% Pseudomonadaceae Bedroom Kitchen Kitchen Kitchen Bathroom Front Door Floor Floor Counter Light Door Knob Knob % Total Population 80% Switch 60% 40% 20% 0% Tim e
  • 19. HOUSE 2 RESULTS Person 1 Person 2 Hand Nose Foot Hand Nose Foot 100% 80% 60% 40% 20% 0% Bedroom Kitchen Kitchen Kitchen Bathroom Front Door Floor Floor Counter Light Door Knob Knob 60% Switch 50% 40% 30% 20% 10% 0% Staphylococcaceae 50% Mycoplasmataceae Moraxellaceae (A) Corynebacteriaceae Streptophyta Moraxellaceae (B) Streptococcaceae 0% Actinomycetales Pseudomonadaceae
  • 20. HOUSE 3 RESULTS Person 1 Person 2 Person 3 Hand Nose Foot Hand Nose Foot Hand Nose Foot 100% 80% 60% 40% 20% 0% Bedroom Kitchen Kitchen Kitchen Bathroom Front Door Floor Floor Counter Light Door Knob Knob 80% Switch 60% 40% 20% 0% Staphylococcaceae 50% Mycoplasmataceae Moraxellaceae (A) Corynebacteriaceae Streptophyta Moraxellaceae (B) Streptococcaceae 0% Actinomycetales Pseudomonadaceae
  • 22. HAND MICROBIAL COMMUNITIES Individuals from different houses Individuals from the same house
  • 26. LIVE UPDATES  HomeMicrobiome.com  Facebook.com/HomeMicrobiome  Twitter.com/HomeMicrobiome  Alldata publicly available
  • 27. ACKNOWLEDGEMENTS  Jack Gilbert – P.I.  Jarrad Hampton-Marcell – Sample Preps  Sarah Owens – Sequencing Manager  Alfred P. Sloan Foundation - Funding