1. Pregnancy Diagnosis in Cow

2. Accurate diagnosis of pregnancy is of crucial importance in maintaining optimal reproductive performance of cattle and high profitability of dairy farms. It is most beneficial if non-pregnancy can be detected before the first heat after insemination. Conventional methods that allow early detection of pregnancy are not accurate enough, and reliance on these methods results in a large number of non-pregnant cows assumed to be pregnant and some pregnant cows being inseminated inappropriately, which could abort them. Conventional pregnancy detection methods that are accurate, on the other hand, can be performed only after at least 35 days after insemination, leading to the loss of at least one opportunity to inseminate Introduction

4. COMPARISON OF PREGNANCY DETECTION TECHNIQUES

7. Test Procedure

15. Result

16. Pregnant Test well (T) same as or lighter than control well (C)‏ Not Pregnant Test well (T) bluer than control well (C)‏

18. If heat is incorrectly interpreted, and insemination is done at the wrong time, the test will show the result as ‘pregnant’ as we are actually detecting the high progesterone level during mid-heat, instead of that caused by pregnancy. A cow that is correctly judged pregnant might lose her conceptus due to various reasons. In this case, the test is accurate, but the pregnancy does not result in calf-birth. It is suggested that the test be repeated if any sign of heat is seen after the cow is judged pregnant A cow with luteinized cystic follicles will produce progesterone at varying levels, and the test result is not reliable in such animals as it might show positive or negative based on the progesterone level on the day of test.

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