1. easyEST MT
Automation-Compatible
Embryonic Stem Cell Toxicity Test
Toxicology Products
» Simpler and much more reliable embryoid-
body production
» No manual turning of culture dishes
» Direct transfer of EBs – fast, easy and no
manual picking!
» Easy scale-up of EB production

2. What is the EST?
The Embryonic Stem Cell Test (EST) is an assay used to evaluate embryotoxicity and
teratogenicity of compounds, cosmetics and chemical applications. The European
Centre for the Validation of Alternative Methods (ECVAM) has validated an EST
protocol, which is widely used. The EST requires the production of mouse
embryoid bodies (EBs) by the classic hanging-drop method which is generally
viewed as a time-consuming, labour-intensive and delicate process. After formation
the EBs are hand-picked and transferred into a multiwell plate for differentiation
into beating cardiomyocytes.
How does InSphero’s easyESTTM make the test more efficient?
InSphero’s easyEST kit* is based on InSphero’s patent-pending culture technology,
which allows the formation of 96 hanging drops by top loading into the plate. No
turning of the culture dish is necessary and the formed EBs can easily be trans-
ferred into a 96-well culture plate for the downstream assay. The easyEST system
is compatible with every multi well-compliant liquid handling device ranging from
a manual single channel pipette to a robotic 96-tip dispenser. The easyEST process
is extremely reliable and time saving, and finally makes your EST fully automation
compatible.
* The easyEST system is not yet validated by ECVAM

3. And the beat
goes on Advantages of InSphero’s easyEST
Faster and easier: No manual turning of the culture dish – top loading into the easyEST system
directly forms stable hanging drops.
No manual picking: Cultured EB can be directly transferred into a 96-well plate for your downstream
assay, no time-consuming manual picking involved.
Automation compatible: The easyEST system can be loaded by any liquid-handling equipment,
robotic, semi-automated or manual.
Highly reproducible: The precise drop-formation process in the easyEST system assures a size variation
below 10% and makes your EST much more robust.
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www.insphero.com

4. Services Overview
For feasibility studies or training for
using our easyEST system please
contact our sales department at
sales@insphero.com
Product Overview
easyEST kit for EB production
Cat. # Name Description
CS-01-001 easyEST kit Hanging-drop cell culture plate, 96 wells, gamma sterilized,
with evaporation control, lid, bottom plate and flat bottom
receiver plate. For use with EST only. InSphero AG
Technoparkstrasse 1
CH-8005 Zurich
Switzerland
www.insphero.com
Phone: +41-44-515049-0
e-mail: sales@insphero.com
All technical and pricing information subject to change.
All products are for research use only. They are not intended for any animal
or human therapeutic or diagnostic use unless otherwise stated.