1. BioCision
Consistent.
Reproducible.
Standardized.
January 2011

2. CoolProducts
CoolProducts enable biological sample consistency,
reproducibility and standardization – user to user, site to
site, trial to trial.
CoolRack® CoolBoxTM CoolCell®
Error-Free / Ice-Free / Alcohol-Free /
Temperature Control Sample Handling Cell Freezing
2

3. Problem: Lack of Standardization
“The lack of standardized, high-quality biospecimens has
been widely recognized as one of the most significant
roadblocks to the progress of cancer research.”
- NIH / NCI / OBBR
• Worldwide effort to develop new standards for collecting,
handling, and processing pre-analytical samples (blood,
tissue, tumor, other biological specimens or sample
materials)
• EU: http://www.devicelink.com/ivdt/archive/09/04/003.html
• NCI/NIH: http://biospecimens.cancer.gov/practices/default.asp
• NIH Office of Biorepositories and Biospecimen Research 2010 Symposium: http://
www.brnsymposium.com/meeting/brnsymposium/2010/
• Duke: http://web.genome.duke.edu/cores/biorepository/protocols/
• Molecular Epidemiology Workshop, Shanghai, 2008
3

4. Solution: CoolProducts
Reproducible and standardized cooling, (snap)freezing, thawing
CoolRack in
water bath
CoolRack
in dry ice
Five consecutive freeze/thaw cycles using a CoolRack module show
highly reproducible freezing and thawing profiles
Biotech International (November 2010)
4

5. How does it work?
• CoolRack modules are made of a novel thermo-
conductive alloy material
• Replaces “direct immersion” into temperature
sources such as ice, dry ice, LN2, water bath
• Thermo-conductivity is the transfer of heat from
higher temperature region to lower temperature
region
• CoolRack on Ice: Heat from the relatively warmer
CoolRack module is transferred to the ice (dry ice,
LN2) until equilibrium is reached
• CoolRack in Water Bath: Heat is transferred from
water bath toward relatively cooler CoolRack until
equilibrium is reached
• CoolRack novel alloy evenly distributes the
temperature across all wells providing very uniform
and consistent temperature to all samples
5

6. CoolRack
CoolRack
Error-Free /
Cooling, Freezing or
Thawing
6

7. Ice Cooling
“BioCision’s proprietary and patented portable bench top tools reduce
variability and ensure consistency in clinical sample handling. How
samples are handled prior to analysis is critical to the outcome of experiments in
basic and clinical research. "Biotech International” (November 2010)
Current method of cooling samples in ice. The CoolRack modules instantly introduce
random layout can result in mis-identification, organization and consistent temperature to all
errors in aliquoting and temperature variability. samples (+/- 0.1oC).
7

8. CoolRack
Versatile
• CoolRack rapidly adapts to any temperature
• from -150oC through 100oC+
• cooling (ice), snap-freezing (dry ice, LN2),
• thawing/incubating (water bath, heat block)
Uniform temperature
• +/-0.1oC temp uniformity to all wells
• standardize assay to assay, user to use, site to site
Patented novel alloy construction
• very rapid thermo-conductivity/adaptivity
• temperature assurance – no mess, no uncertainty
8

9. Modular
For a variety of biological specimens
CoolRack
tube holders
CoolSink
Plate holders
CoolTray
platforms
9

10. Versatile
For a variety of applications
-150oC -78oC 0o to 4oC 37oC +
Liquid Nitrogen Dry Ice Ice Water Bath
• Eliminate direct immersion • Replaces direct contact with • Gain organization & stability • Keeps tubes out of water –
into LN2 dry ice in ice reduced contamination, labels
• All tubes are upright • Eliminates ethanol bath on • Even temperature distribution stay dry
• Same freezing profile for all the bench – cost savings, to all samples even as ice melt • Consistent thawing profile for
samples reduced hazard & waste • Labels stay dry and legible for all samples
• ThermalTray provides • CoolRack PF provides equal better sample identification • All wells at identical
elevation of samples & or faster snap-freezing than • Reduced risk of sample temperature - eliminates edge
increased distance between ethanol slurry contamination effect on multi-well plates
hands and LN2 • Standardized freezing - • Rapidly goes from ambient to • Stable support of tubes &
• Rapidly adapts from ambient minimized standard deviation in <4oC in 90 seconds plates
(25oC) to LN2 temp (-150oC) in freeze profiles • Using ThermalTray, 10-15
12-14 minutes • Improved work flow hours of <4oC with one pan of
• Adapts to -78oC in 5-7 ice
minutes
BioCision’s tools are unique and replace very rudimentary methods of sample preservation
(freezing, snap-freezing) and handling (cooling, thawing)
10

11. CoolRack Performance
Cooling Rate - Ice
§ Ambient to < 4oC in 60-90 seconds
§ 4-6 hours of use when placing CoolRack directly on
ice
§ Up to 14 hours cooling with a single pan of ice with
ThermalTray
Cooling rate on ice
Snap-Freezing Rate – Dry Ice
§ Equal or better freeze rate than dry ice/
ethanol bath
§ No alcohol required – cost savings, reduced
hazard
§ Consistent freeze profile for all samples
Freeze rate on dry ice Standard deviation
11

12. CoolSink Performance
Ø Multi-well plates placed directly on ice for
cooling do not achieve critical < 4oC
temperature in any of the wells (Fig. 2, A)
Ø CoolSink modules evenly distribute the ice
temperature to all wells (Fig. 2, B and C)
• All wells are <4oC
• Curvature of the plate accounts for center
well variability (Fig.1)
Figure 1. Variance in height of undersurface of 96-well flat- Figure 2. Final equilibrium well temperature for a 96-well flat bottom plate
bottom plate. The x and y axes represent the well number in direct contact with (A) crushed ice, (B) CoolSink 96Fwith a dry interface,
and letter, and the z axis represents the relative deviation of and (C) CoolSink 96F with aqueous conductive medium in the interface.
the surface in units of 0.001 in. Data was collected using a Colors represent 0.5°C temperature intervals of the corresponding plate
ball dial indicator with a resolution of 0.0001 in. wells. The white cell represents the well that was fitted with the
thermocouple probe. Published in Biotechniques, November 2010
12

13. Kits and Accessories
• CoolBox
• Ice-free cooling container
• CoolSystem
• Include ThermalTray LP, CoolRack(s),
large ice pan
• Accessories
• Ice pans
• Rectangular (9L & 4L) and round (4L)
o
• Temperature strips (1-8 C)
• LCD display adheres to all thermo-conductive modules
• CoolMat histology cutting mat
• Protects surface of ThermalTray and scalpel blade
13

14. Customer Feedback - CoolRack
Steffan Vartanian - Genentech
Don’t you hate it when you spend all morning preparing RNA or harvesting lysates, only to
come back from lunch to find your precious samples drowning in a pool of melted ice? ……
But after using the Thermal Tray and CoolRack a few times, my soggy-sample days are
officially over! Not only do these tools keep your samples chilled for hours, but they also
keep everything organized, safe, and secure. BioCision's products are very well made, well
designed, and just plain look cool. Highly recommended!
Hans B - Perkin Elmer
We have been using the CoolRack for snap freezing our samples for the past weeks with
great success. Since we were on a tight schedule we performed some validation in house
and were very pleased with the results.
14

15. CoolBox
CoolBox
Ice-Free /
Sample Handling
15

16. CoolBox
Consistent ice-free cooling and freezing
• cartridges provide up to 10 hours of ice-free cooling or freezing
• continuous +/- 0.1oC consistency to all wells
Versatile
• five temperature options using cartridges,
dry ice or LN2
• portable
• accommodates variety of tubes & plates
Highly recommended for
• PCR and RNA work, cell culture, cooling in biosafety cabinet, GMP/GLP
suites and vivarium, bio-hazardous containment, short transport
16

17. CoolBox 30 & CoolBox Microplate
CoolBox Microplate System
• Ice-free SBS-format plate & tube cooling &
freezing
o
• Includes CoolBox, lid, blue +2 C cartridge
• Accommodates all SBS format modules -
CoolSinks, CoolRack PCRs, CoolRack 1mlx96
• Sold separately OR with CoolRack/CoolSink
CoolBox 30 System
• Ice-free tube cooling / freezing
o
• Includes CoolBox, lid, blue +2 C cartridge, red
stage, purple insulator pad
• Accommodates all 30-well CoolRacks
• Sold separately OR with CoolRack
17

18. CoolBox Performance
mmmm
-25oC with thermal regulator
pad placed between CoolRack
and dry ice
Snap-freezing at -78oC with
CoolRack directly on 200cc dry
ice
NOTE: Performance may vary depending on ambient
conditions, starting temperature and other specific set-up
criteria. See instructions for complete information.
18

19. CoolBox Advantage
CoolBox Gel-Filled Container
Well-to-well Temperature Variance: Well-to-well Temperature Variance:
+/-0.1oC Up to 4oC
Cooling Media: Cooling Media:
LN2, Dry ice, Wet ice, Cartridge Gel
Temperature range: Temperature range:
-150oC through +150oC Fixed at -20oC or 0oC
Tube & Plate Formats: Tube & Plate Formats:
0.5ml conical, 1.5ml conical, 1.5-2.0ml 0.5ml conical, 1.5ml conical, PCR tubes
cylindrical, 12.5mm dia cryos, mass
spec tubes, PCR 96- and 384-well
plates, 6-, 12-, 24-, 48-, 96-well plates
Cooling Duration: Cooling Duration:
10h (2ºC), 6h (-12ºC), 5h (-25ºC), 8h (0ºC), 3h (-21ºC)
6h (-78ºC)
Down Time: Down-Time:
None – switch cartridge with another 4-6 hours to re-freeze gel-filled tube rack
frozen one from the freezer or replenish
dry ice, LN2
CoolBox provides temperature uniformity and versatility
19

20. Customer Feedback - CoolBox
Fraser Moss Ph.D. - Case Western Reserve University, Dept. of Physiology and
Biophysics, President Scientist Solution
BioCision has now eliminated the ice factor and have the CoolBox in which you can keep the
96 well CoolRack…..I'm using it to keep my RNA cold for oocyte injections right now. I'll be
using it to assemble my PCR reactions in the not to distant future. This has been a great new
little toy to have on the bench recently because our departmental ice machine broke in April
and still is not fixed!
Laura Matthews - Sarah Cannon Research Institute (SCRI)
I am writing regarding the demo CoolRack and CoolBox that we have been testing. They are
both great products. The CoolRacks have eliminated the mess of mushy cardboard freezer
boxes. The samples also freeze much faster in the CoolRacks. The CoolBox was especially
helpful in the transport of fresh biopsies on dry ice from one location to another
20

21. CoolCell
CoolCell
Alcohol-Free /
Cell Freezing Container
21

22. CoolCell
Highest reproducibility
• -1oC/minute freeze rate
• Every freeze run is exactly the same
• Identical freezing profiles every time
Highest cell viability
• Cell lines, PBMC, primary cells, stem cells, yeast
Alcohol-free
• No alcohol – no fluids
• No on-going alcohol expense or waste
• No maintenance
• No variability due to changes in alcohol
22

23. CoolCell
Eliminates alcohol-induced freezing variation
• Many researchers currently use alcohol-filled containers to
achieve a -1oC/minute freeze rate when freezing cells in
-80oC freezer
Alcohol-filled container
• The alcohol must be replaced every 5 uses because the
alcohol changes each time it’s frozen
• Alcohol absorbs moisture and evaporates
• CoolCell uses no alcohol (no fluids)
• Nothing changes
• Identical cell freezing results every time
23

24. Reproducible Results
Five consecutive freeze cycles in CoolCell show fusion
time and cooling profiles are identical every time
24

25. High Cell Viability
In primary human cells, CoolCell significantly
outperforms
25

26. CoolSystemTM CRYO
• Kit for ice-free vial preparation in a laminar flow hood & alcohol-free cell
freezing in a -80oC freezer
• CoolBox CFT provides ice-free 0-4oC work station for 30 cryo vials and
allows one-handed vial opening and closing
• CoolCell provides alcohol-free and standardized cell freezing
CoolSystem CRYO (#BCS-142) includes CoolBox CFT30 System and CoolCell
26

27. CoolCell Advantage
CoolCell Alcohol-Filled Container
Very consistent -1oC/min rate Inconsistent freeze rate
• All vials have uniform freeze rate • Each run is different due to alcohol
every time degradation
• Radially symmetric – design enables • Two circles of wells – different freeze rates
consistency for each circle
No alcohol Requires 250ml alcohol
• No hazardous fluids • Replace alcohol every 5 uses (250ml)
• Solid core and insulation design • Keep track of # of uses
• No pre-cooling required • Alcohol degrades slightly each time it s
used
• Pre-cool alcohol in refrigerator
Cost savings ~$338/year maintenance per unit
• No maintenance cost • Change alcohol weekly (~$6/wk)
• Pays for itself quickly • Dispose haz waste (~$0.75/wk)
• 10 units = $3,380 to use EVERY YEAR
Lid opens easily Screw cap very difficult to remove when
• Not cold to the touch when removing frozen
from the -80C freezer • Protective glove required when handling
frozen unit
Better cell viability Good cell viability
Ready to use again in 5 minutes Takes >1 hour for device to return to
room temp for re-use
Low impact on freezer Large thermal mass impacts local
• 1/3 the heat impact on freezer freezer area
compared to alcohol-filled units • Large heat capacity removed from alcohol
impacts nearby samples
27

28. Customer Feedback - CoolCell
Rohit G. - Stanford University
We run a registry, in which large amounts of PBMCs are processed for long-term
cyropreservation.………Overall, the CoolCell has proven to us to set a new bar in
cryopreservation.
Srujan P. - Merck Pharmaceutical, Vaccine Manufacturing
I have already purchased 21 CoolCells, I have obtained a CoolBox, and I recently ordered a
96well flat bottom CoolCell system. All these will be used for our assays…. I already passed
on my evaluation of the cool cell and the other groups are pretty excited and are going to use
them.
Sam Knight - Ceramisphere (Australia)
Purely altruistically, I wanted to mention that one big advantage of the CoolCell is that you can
use dry ice to do your freezing if you don't have a -80. I spent nearly a year getting terrible
viability-recovery on cell freezing with dry ice (in an alcohol device) followed by storage in LN2,
and this on HeLa cells which aren't exactly hard to store. I then bought a CoolCell and now I
can just use standard cryo-preservation techniques (DMSO, 20% NBS) and get nearly 100%
viability on thawing.
28

29. Summary
29

30. Standardizing Clinical Sample Handling
• Clinical sample handling has become increasingly important
• growing number of multi-national clinical trials
• increasing use of biomarkers
• the move toward personalized medicine.
• BioCision products address common and often overlooked
inconsistent procedures in every laboratory
• Non-standardized methods of cooling/freezing/thawing
• Consistency. Reproducibility. Standardization.
• Test to test
• Researcher to researcher
• Lab to lab
• Site to site

31. Visit www.biocision.com
• Product data & brochures
• Applications
• Technical articles
• Videos
• Customer testimonials
• Customization
31

32. Genentech Amgen Pfizer Roche Harvard MIT
CalTech UCSF Glaxo Bristol Myers Squibb Novartis
Translational Genomics Gilead Sciences Merck
MedImmune ATCC Genpharm Elan Pharmaceuticals
Centocor Boehringer Ingelheim Oncomed NIH/NCI
Takeda Pharmaceuticals CDC Covance XOMA
Thank you
Quest Diagnostics Otsuka America Pharmaceuticals
Salk Institute Burnham Institute Scripps Institute
Stanford University Yale Genzyme EMD Serono
Shire HGT Alnylam Wyeth Cell-Systems
Jackson Labs Beth Israel Deaconess Hospital HHMI
Whitehead Institute Harvard Systems Biology
Exelixis Charles River Labs Sanofi Pasteur Penn State
Lawrence Berkeley Lab Batelle UC Berkeley
Cedars Sinai Hospital Cleveland Clinic IDEXX Biogen
Perkin Elmer Genetics Blood Systems Research Inst.
Copyright 2011. Patents pending. All rights reserved.