This document provides details about the biocontrol laboratory located at the K.K. Wagh College of Agriculture in Nashik, India. It describes the 14 rooms that make up the laboratory, including rooms for sterilization, media preparation, inoculation, fungal growth, storage, mixing, insect rearing, virus production, and washing. The laboratory is used to mass produce various biocontrol agents like Trichogramma, Cryptolaemus, and fungi such as Beauveria, Metarhizium, and Trichoderma for use in pest management. Students conduct practical activities in the laboratory, such as rearing host insects and producing biopesticides like NPV viruses.

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4. Introduction To Bio
Control
Laboratory

5. Location of BCL:-
 Name of department :-Agril. Entomology
 Name of college :- K. K. Wagh college of agril.
Nashik-03
 Name of farm area:- Puriya park, Saraswati nagar
Panchvati Nashik-03
 Name of university :- M.P.K.V ,Rahuri

6. External view of bio control
laboratory

7. Experiential learning Module Activity
Course No. AEL-ENT-486
Title : Mass production of bioagent and biopesticides
Credits=0+20
Major Department: Agril. Entomology
Associated subjects: Economics, Extension Education
No. of students Registered: 14
Activities carried out by students:
Rearing of host insect like Corcyra cephalonica, Mealy bug
Rearing of Bioagents like Trichogramma, Cryptolaemus,
Zygogrmma etc.
Mass production of HaNPV and SlNPV
Mass production of Metarhzium, Nomuraea, Verticelium,
Beavaria,Trichoderma & Psudomonus etc.

8. No. of cells of Biocontrol lab :-
1) Sterilization room
2) Media room
3) Inoculation room
4) Growth room 1
5) Growth room 2
6) Store room
7) Mixing room1
8)Mixing room2
9) Insect rearing room1
10) Insect rearing room2
11)Bio agent room1
12)Bio agent room2
13)NPV room
14)Washing room

9. STERILIZATION ROOM :
1) use of this room for
sterilization through the
Autoclave.
2) This room should be
well ventilated by fitting
exhaust fan.
3) In this room should be
2 autoclave, a
horizontal & a vertical
autoclave & Hot air
oven.

10. For maximum
flexibility, autoclave
space is
recommended on
each floor, or at a
minimum in a
convenient location
in each building,
where
microbiological
research is
performed.AUTOCLAVE

11. Media preparation room :
This room should get a sufficient & continuous
supply of single & double-distillation unit ,pressure
cooker , Exhust fan.

12. Inoculation room :

13. Inoculation room :
1) In this room the inoculation cabinet laminar air flow can be
kept.
2) The floor of this room should be covered with linoleum or
tiles to facilitate proper cleaning.
3) It is essential that the atmosphere in this room should be
free from contaminants.
4) This room should preferably be air-conditioned because it
ensures good air-circulation & helps in maintaining
constant temp.

14. GROWTH ROOM :

15. GROWTH ROOM :
1)In this room all inoculated bottles with spores
are kept.
2)This room should be in sterile condition
3)Air condition should be there to control
temperature.
4)The following fungi are grow in this room :
a)Verticillium lecanii
b)Beauveria bassiana
c)Nomuraea rileyi
d)Metarrhizium anisopliae
e)Trichodemma spp.

16. STORE ROOM :
1) In this room store in Chemicals
2) Then Chemicals are Agar agar,
ethyl alcohol, peptone,
streptomycine, talc powder
etc.
3) The chemical rack should be
arrange with A to Z
alphabetical latters so its easy
to find chemicals.

17. MIXING ROOM
1) Grinding of different
fungus like
trichoderma,verticilium,
beauveria,metarhizium
etc.
2) Mixing the mycelium
culture with talcum
powder
3) Drying of blended
culture
4) Equipment: laboratory
blander
two types of microscope
I. Steriozoom microscope
II. binocular microscope

18. INSECT REARING ROOM :
1) In this room rearing of
helicoverpa larvae &
spodoptera .
2) Rearing of moth of
helicoverpa & spodoptera
moth in glass chamber.
3) Rearing of mealy bug on
pumpkin as a food for
cryptolamous its use in off
season when the mealy
bug can not be available.

19.  MICROVAVE OVEN :
 Microwave oven use to heat the content of
artificial diet.

20. NPV ROOM :
1) This room is also called as virus
room.
2) All viral processed taken in this
room.
3) NPV made in this room.
4) The Infected food i.e.
.gram, A.diet given to the
helicoverpa larvae given in this
room.
5) Equipment : centrifugal,
laboratory blender, rack,
exhaust, fan & furniture.
6) ventilation system provide in
this room for exhaust of dirty
smell.

21. WASHING ROOM :
1) The room should be
provided with necessary
service lines as water
,cleaning materials i.e.
soap, sodium chloride .
2) In two side of the room a
washing sink can be
fitted.
3) The drainage & drying
racks could be
accommodated in this
room.

22. THANK YOU