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Recombinant DNA technology

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Recombinant DNA technology

  1. 1.
  2. 2. Today we will<br />Understand what Diabetes is and how it affects the person<br />Understand how insulin can be produced by recombinant DNA technology<br />
  3. 3. The problem - Diabetes<br />What do you know?<br />Symptoms? <br />Cause? <br />Treatment?<br />
  4. 4. Insulin– what is it exactly?<br />A hormone (chemical messenger) that allows glucose to pass from the blood into your cells.<br />
  5. 5. How it works<br />
  6. 6. The solution - how can we getb lots of insulin?<br />Other animals?<br />Get something less cute to make it for us using the gene for insulin. <br />
  7. 7. Recombinant DNA technology<br />Joining together DNA molecules from two different species. This is then inserted into a host organism which can produce new genetic combinations that are useful to us<br />
  8. 8.
  9. 9. Bacteria – E. coli<br />Transformation<br />
  10. 10. And this is how…<br />
  11. 11. Key words<br />Isolated gene Sticky ends Vector Plasmid Restriction enzyme DNA ligase<br />Recombinant plasmidTransformation<br />Steps (not in order)<br />The insulin gene is inserted into the bacterial plasmid using DNA ligase<br />The gene for insulin is isolated and<br />removed from human DNA leaving it with sticky ends<br />The recombinant plasmid is <br />taken up by the bacteria by transformation<br />A plasmid from the bacteria E coli is removed and cut open using restriction enzymes<br />The bacteria reproduces, making copies of the gene each time, allowing lots of insulin to be produced<br />
  12. 12. What else?<br />
  13. 13. Hormone<br />Pancreas<br />Vector<br />Plasmid<br />Transformation<br />Gene<br />Insulin<br />Host<br />Recombinant DNA<br />Restriction<br />enzyme<br />DNA ligase<br />Sticky ends<br />

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