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Innovative Immunodiagnostic Solutions
Materials For
Rapid
Diagnostic Kits
5. Absorbent pad
3. Nitrocellulose membrane
2. C...
Introduction
Established in year 2006, Axiva Sichem Pvt. Ltd. is engaged in
manufacturing & exporting a wide range of Diag...
Functional Properties
Protein binding is a critical aspect of Nitrocellulose Membrane performance. To achieve optimum assa...
How to select nitrocellulose lateral flow membrane?
Selection of LFT membrane depends upon assay.
Highest sensitivity is o...
Ordering Information
Cat. No. Rating Wicking Time Width Length
DE20/SX04 8 µm 160 sec 20 mm ± 0.3 100 ± 2 meter roll
DE25/...
Ordering Information
Cat. No. Rating Wicking Time Width Length
CG20/SX02 12 µm 120 sec 20 mm ± 0.3 100 ± 2 meter roll
CG25...
Axiva Sample Pad:
Sample pad is the platform where sample / analyze is placed at the time of testing.
Sample pad is one of...
Axiva Conjugate Release Matrix Pad.
The conjugate release matrix pad is made of a non-absorbent material such as fiberglas...
Absorbent Pad
The absorbent pad, also called a wick or wicking pad, pulls fluid off of the membrane to allow the capillary...
Comparative Studies
Comparative studies of Axiva Nitrocellulose Lateral
flow membrane 15 micron and competition.
Malaria A...
Axiva PES Membrane Filter
PES is hydrophilic and is made of Polyethersulfone polymer membrane.
Specific features
Extremely...
Cross Reference Guide
Cross reference guide Nitrocellulose Lateral Flow Membrane
Axiva Millipore mdi Sartorious Whatman
CH...
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Axiva Sichem Manufactured Best Quality Diagnostic Products!

Axiva Sichem Pvt.Ltd is one of the leading manufacturer and exporter of diagnostic products like as Nitrocellulose Lateral Flow Membrane, Sample Pad (Glass Fibre), Conjugate Pad (Polyester Pad) and Absorbent Pad (Cellulose Fibre). The offered products are examined on set excellence parameter in order to deliver superior range to the clients.

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Axiva Sichem Manufactured Best Quality Diagnostic Products!

  1. 1. Innovative Immunodiagnostic Solutions Materials For Rapid Diagnostic Kits 5. Absorbent pad 3. Nitrocellulose membrane 2. Conjugate pad 1. Sample pad 6. Plastic adhesive 4(b). Control line backing card 4(a). Test line
  2. 2. Introduction Established in year 2006, Axiva Sichem Pvt. Ltd. is engaged in manufacturing & exporting a wide range of Diagnostic products like 1. Nitrocellulose Lateral Flow Membrane 2. Sample Pad (Glass Fibre) 3. Conjugate Pad (Polyester Pad) 4. Absorbent Pad (Cellulose Fibre) 5. Membrane Filters Nitrocellulose Membrane Nitrocellulose Membrane is the most important part of a lateral flow assay and require highly consistent measurements for capillary flow rate and thickness across the length and width. Variation in either can have detrimental effects on assay sensitivity, reproducibility, repeatability in results and reagent consumption (in special assay developments which are controlled by time or volume consumed). Signal generation at the test and control lines is influenced by the thickness of the reaction membrane. The variation in thickness across or down a roll can have negative effects in both the manufacturing in-process approval levels and final assay performance. For maintaining the uniformity of membrane Axiva posses a complete in house developed manufacturing unit for membrane and well developed measuring techniques which ensure release of product with stringent specification and CV for the best performance under critical assay developing processes Consistency By Design The Team A group of skilled scientists, technicians, production workers and management are contributing their ability in a team work for developing a membrane technology for Lateral Flow Assay Test. The Application Lab Our diagnostic membrane application lab is fully equipped with instruments and material that enable us to characterize diagnostic membrane. To evaluate for Lateral Flow Test almost common chemistries and parameters are used in production and in laboratory. Manufacturing Facilities Axiva using a complete clean room in-house production facility well equipped with instruments. We have rigid environmental controls in place to produce a stable and clean manufacturing process. Complete area is class 100000, GMP compliant. Membrane Manufacturing Process The solution mixing and casting processes are designed and controlled to provide outstanding inter and intra-lot consistency. AXIVA owns clean-room facility for production of membrane which ensures a dust free smooth membrane surface. Consistency By Testing Physical Testing Capillary wicking time is simple and highly informative test. 50μl of normal saline solution dropped at one edge of membrane strip and it wicks towards an open edge. Time taken to reach a fixed distance is called wicking time in seconds. Axiva membranes are characterized and differentiated by measurements of Wicking times of well planned samples and pore size comparisons with microscope. So we have traceable, repeatable and consistent quality products with well defined wicking times in seconds with isotonic normal saline solution. Signal generation at the time of test, control lines and tensile strength are largely influenced by the thickness of the reaction membrane. The variation in thickness across or down a roll can have negative effects in both the manufacturing environment and final assay performance. For maintaining the uniformity of membrane Axiva posses a complete in-house manufacturing for membrane and measurements are highly controlled with specification and CV for the critical parameters of membrane. 2
  3. 3. Functional Properties Protein binding is a critical aspect of Nitrocellulose Membrane performance. To achieve optimum assay results, the membrane must bind sufficient proteins at the capture lines but not non-specifically bind the target analysts in the sample. Assay reproducibility is facilitated by consistent levels of protein binding which translates to achieving the desired amount of antibody binding at the capture lines. If there is variability in protein binding at the capture lines, the assay sensitivity and crispness of the capture line can be compromised. Axiva produces a uniform and good binding membrane that better for assay testing. Consistent Wettability Although cellulose nitrate polymers are naturally hydrophobic, Axiva use specific surfactant for treatment, to get a long time uniform wetting property of membranes. Also stability studies are done over a period of time on the basis of accelerated heat stability test Data of storage at normal storage conditions 4cmWickingTime(Sec) Good Quality's that increase the assay reproducibility and sensitivity of membrane • Strong consistency – Membrane provides high sensitivity and low background in diagnostic assays due to its consistent nature. • Increased sensitivity – Provides high sensitivity with dilute concentrations of target analytes with no false signals • Clear results – Demonstrates low background levels which enable crisp capture lines and easy-to-read results. • Dependable performance – Constructed of pure nitrocellulose that does not contain interfering substances or post-treatments that affect assay performance. 3
  4. 4. How to select nitrocellulose lateral flow membrane? Selection of LFT membrane depends upon assay. Highest sensitivity is obtained with smaller size and slower wicking time/capillary flow time. Axiva offer wide range of Nitrocellulose lateral flow membrane to meet the requirement of different tests. Wicking Membrane Sensitivity Flow Rate Line Applications time/Capillary Micron rating intensity flow time 160 ± 35 8 µm Highest Slower Sharp Milk test, sec/40 mm Urine Ab test, distance HbsAg serum test 140 ± 30 10 µm Medium Medium Sharp Dengue, Malaria sec/40 mm Infectious disease distance screening, Environmental and agricultural analytes. 120 ± 28 sec/ 12 µm Medium Fast Sharp Heart disease markers, 40 mm medium Drug of abuse monitoring distance Infectious disease tests, Syphilis, Food and beverage testing 110 ± 25 15 µm Lower Faster Less sharp Whole Blood tests, sec/40 mm Monitoring ovulation, distance Dengue and infectious disease. 100 ± 25 15 µm Lower Faster Less sharp Pregnancy test. sec/40 mm distance 1. Axiva Nitrocellulose Membrane 160 sec (8µm) This membrane is used for testing of urine Ab tests and for HbsAg serum test. Specification and characteristics Backing Specification Membrane Specification Material Polyester Material Cellulose Nitrate Polymer Thickness 102 ± 5µm Thickness 105 ± 15µm (Membrane layer only) Colour Transparent Wicking Time 160 ± 35 sec/40mm distance Wetting Time ≤ 5 sec (10µl volume 0.3% BAS in PBS 0.01M pH 7.4) Roll Specification Roll Core 76.5 mm = 3" (inner Diameter) Core Material Plastic Available Membrane width As desired 4
  5. 5. Ordering Information Cat. No. Rating Wicking Time Width Length DE20/SX04 8 µm 160 sec 20 mm ± 0.3 100 ± 2 meter roll DE25/SX04 8 µm 160 sec 25 mm ± 0.3 100 ± 2 meter roll DE30/SX04 8 µm 160 sec 30 mm ± 0.3 100 ± 2 meter roll DE40/SX04 8 µm 160 sec 40 mm ± 0.3 100 ± 2 meter roll Custom dimension are also available on request 2. Axiva Nitrocellulose Membrane 140 sec (10µm) This membrane is used for testing of Dengue, Malaria and for infection disease tests with serum etc. Specification and characteristics Backing Specification Membrane Specification Material Polyester Material Cellulose Nitrate Polymer Thickness 102 ± 5µm Thickness 105 ± 15µm (Membrane layer only) Colour Transparent Wicking Time 140 ± 30 sec/40mm distance Wetting Time ≤ 5 sec (10µl volume 0.3% BAS in PBS 0.01M pH 7.4) Roll Specification Roll Core 76.5 mm = 3" (inner Diameter) Core Material Plastic Available Membrane width As desired Ordering Information Cat. No. Rating Wicking Time Width Length DF20/SX04 10 µm 140 sec 20 mm ± 0.3 100 ± 2 meter roll DF25/SX04 10 µm 140 sec 25 mm ± 0.3 100 ± 2 meter roll DF30/SX04 10 µm 140 sec 30 mm ± 0.3 100 ± 2 meter roll DF40/SX04 10 µm 140 sec 40 mm ± 0.3 100 ± 2 meter roll Custom dimension are also available on request 3. Axiva Nitrocellulose Membrane 120 sec (12µm) This membrane is used for testing of hCG, and for infection disease tests with serum etc. Specification and characteristics Backing Specification Membrane Specification Material Polyester Material Cellulose Nitrate Polymer Thickness 102 ± 5µm Thickness 105 ± 15µm (Membrane layer only) Colour Transparent Wicking Time 120 ± 28 sec/40mm distance Wetting Time ≤ 5 sec (10µl volume 0.3% BAS in PBS 0.01M pH 7.4) 5
  6. 6. Ordering Information Cat. No. Rating Wicking Time Width Length CG20/SX02 12 µm 120 sec 20 mm ± 0.3 100 ± 2 meter roll CG25/SX02 12 µm 120 sec 25 mm ± 0.3 100 ± 2 meter roll CG30/SX02 12 µm 120 sec 30 mm ± 0.3 100 ± 2 meter roll CG40/SX02 12 µm 120 sec 40 mm ± 0.3 100 ± 2 meter roll Custom dimension are also available on request 4. Axiva Nitrocellulose Membrane 110 sec (15µm) This membrane is used for whole blood test and for Malaria tests. Due to larger pore size the wicking speed is fast and good for viscous or particle loaded sample. Specification and characteristics Backing Specification Membrane Specification Material Polyester Material Cellulose Nitrate Polymer Thickness 102 ± 5µm Thickness 105 ± 15µm (Membrane layer only) Colour Transparent Wicking Time 110 ± 25 sec/40mm distance Wetting Time ≤ 5 sec (10µl volume 0.3% BAS in PBS 0.01M pH 7.4) Ordering Information Cat. No. Rating Wicking Time Width Length CH20/SX05 15 µm 110 sec 20 mm ± 0.3 100 ± 2 meter roll CH25/SX05 15 µm 110 sec 25 mm ± 0.3 100 ± 2 meter roll CH30/SX05 15 µm 110 sec 30 mm ± 0.3 100 ± 2 meter roll CH40/SX05 15 µm 110 sec 40 mm ± 0.3 100 ± 2 meter roll 5. Axiva Nitrocellulose Membrane 100 sec (15µm) This membrane is used for HCG (Pregnancy Test) Specification and characteristics Backing Specification Membrane Specification Material Polyester Material Cellulose Nitrate Polymer Thickness 102 ± 5µm Thickness 105 ± 15µm (Membrane layer only) Colour Transparent Wicking Time 100 ± 25 sec/40mm distance Wetting Time ≤ 5 sec (10µl volume 0.3% BAS in PBS 0.01M pH 7.4) Ordering Information Cat. No. Rating Wicking Time Width Length CH20/SX01 15 µm 100 sec 20 mm ± 0.3 100 ± 2 meter roll CH25/SX01 15 µm 100 sec 25 mm ± 0.3 100 ± 2 meter roll CH30/SX01 15 µm 100 sec 30 mm ± 0.3 100 ± 2 meter roll CH40/SX01 15 µm 100 sec 40 mm ± 0.3 100 ± 2 meter roll Custom dimension are also available on request 6
  7. 7. Axiva Sample Pad: Sample pad is the platform where sample / analyze is placed at the time of testing. Sample pad is one of the most important elements after the Nitrocellulose membrane in the lateral flow test / immune chromatographic test. It is used to control the p H of sample, movement of the gold conjugate and also block the membrane on the fly. Sometime it is necessary to treat the sample pad to get good assay performance. Axiva Sample pad made of binder free glass fibre that exhibits high absorption capacity. It does not bind proteins. It has excellent resistance to withstand tension and effectively filter components in the sample to prevent analyze contamination. The in vitro diagnostic glass fibre sample pad is available in a wide range of thickness, absorption levels and compositions. Ordering information Cat No. Thickness Composition Applications SP02(03) T 320 – 440 µm 100 % Glass fibre Sample pad/Source pad SP02(06) T 530 – 670 µm 100 % Glass fibre Sample pad/Source pad
  8. 8. Axiva Conjugate Release Matrix Pad. The conjugate release matrix pad is made of a non-absorbent material such as fiberglass pad, polyester, rayon or a similar material. The conjugate pad is always of a synthetic material (at least when using a gold conjugate) to ensure the efficient release of its contents. As its name implies, the assays’ detection conjugate is dried down and held in place here until a liquid test sample is applied to the sample pad. This liquid sample, moves into the conjugate pad by capillary action, re-hydrates the dry gold conjugate, allowing the sample to mix with the conjugate. The complex of gold conjugate and analyte then moves into and up the membrane. Pretreatment of the conjugate pad helps to ensure the conjugate releases at the proper rate and enhances its stability. The pretreatment is performed in the same way as with the sample pad. Features Good uniformity Hydrophilic High absorption Good wicking rate Wide compatibility Ordering information Cat No. Basic weight Caliper Wicking rate Water Nature Composition g/m2 mm s/4 cm absorption mg/cm2 16613 100 0.42 ---- ---- Hydrophobic Untreated Polyester 16613 T 100 0.42 30 27 Hydrophilic Treated Polyesters 16615 135 0.51 — — Hydrophobic Untreated Polyesters 16615T 135 0.51 — — Hydrophilic Treated Polyesters 8
  9. 9. Absorbent Pad The absorbent pad, also called a wick or wicking pad, pulls fluid off of the membrane to allow the capillary flow of the membrane to keep flowing in the proper direction and at the proper rate. If an absorbent pad isn’t used (or if it separates from the membrane so it is functionally absent), the sample and buffer will back flow down the membrane and could raise the background or possibly cause false positives. This can also occur if the absorbent pad selected for the volumes of buffer and sample involved is inadequate. Most absorbent pads are made from non-woven, cellulose fibre sheets. These pads can be manufactured in a variety of thicknesses and densities to suit the needs of the assay. Ordering information Cat No. Size Composition Applications 1222 As desired 100 % cotton fibres Sink Pad/Absorbent pad Technical information Cat No. Basic weight Caliper Wicking rate Water absorption g/m2 mm s/4 cm Mg/cm2 1222 291 0.83 20 88 AXIVA Developers kit Heading - LATERAL FLOW TEST / IMMUNOCHROMATOGRAPHY DEVELOPER"S KIT. is the starting point for those new to lateral flow test as well as for those skilled in the art who want to select the BEST materials for the assay under development. Product Description Sample pads SP(06) Conjugate Pads Conjugate pads -Treated and Untreated. Nitrocellulose Lateral flow membrane 4 different types of nitrocellulose membrane Absorbent pads AP 1222 Masking Tape MT1 and MT2 9
  10. 10. Comparative Studies Comparative studies of Axiva Nitrocellulose Lateral flow membrane 15 micron and competition. Malaria Antigen HRP II PF and pLDH PV. Studies at 45o C for 6 days. Competition Axiva The Major Crux of Axiva Nitrocellulose Lateral Flow Membrane Sensitivity of Axiva Nitrocellulose Lateral Flow Membrane is more as compared to Competition Wicking Rate is also good and uniform No False Positive result Control Line and Test Line are intact 10
  11. 11. Axiva PES Membrane Filter PES is hydrophilic and is made of Polyethersulfone polymer membrane. Specific features Extremely low protein binding results in very high product recovery High Flow Rates Absolute microbial retention Low sample absorption Autoclavable Applications Sterilization / Clarification of protein solution, culture media, serum and high value additive Ordering information Cat No. Size /Dia. in mm Micron rating (µm) Nos./Pkt. 230142 R 142 0.2 µm 50 230142 X 142 0.45 µm 50 Axiva Glass Fibre Filter Pre filters Ideal for pre filtration Ordering information Cat No Dia.(mm) Micron rating (µm) Nos./Pkt. GFP14215 142 1.5 50 GFP14220 142 2.0 50 Axiva PES Syringe Filters- Sterile (Individually packed) Axiva offers Syringe filters for the filtration of small volume and high value products such as GOLD CONJUGATE and ANTIBODIES PES is low protein binding and ideal for high value antibodies and reagents. Ordering information PES SFPS25R 2017-12 Cat No Dia.(mm) Micron rating (µm) Nos./Pkt. SFPS13 R 13 0.2 100 SFPS13 X 13 0.45 100 SFPS25 R 25 0.2 50 SFPS25 X 25 0.45 50 11
  12. 12. Cross Reference Guide Cross reference guide Nitrocellulose Lateral Flow Membrane Axiva Millipore mdi Sartorious Whatman CH25/SX05 HF090 CNPC-SS12 (15µ) UN95ER100025NT 10547003 CH25/SX01 HF075 HCG-1 CG25/SX02 HF120 CNPC-SS12 (12µ) 10547001 DF25/SX04 HF135 CNPC-SS12 (10µ) UN14ER100025NT 10547004 DE25/SX04 HF180 CNPC-SS12 (8µ) UN15LR100025NT Cross reference guide Sample pad. Axiva Ahlstrom mdi SP02(03)T 141 GFB-R4(0.35) SP02(06)T 121 GFB-R4(0.6) Cross reference guide Conjugate Release Matrix Pad Axiva Ahlstrom mdi 16613 6613 PT-R 16613T 6613H PT-R2 16615 6615 PT-R5 Cross reference guide Absorbent Pad Axiva Ahlstrom Millipore mdi 1222 222 C083 AP080 Axiva Sichem Pvt. Ltd. Delhi (India) Ph.No: +91-11-40578224/25/26 Email: arvind@axivasichem.com Web: www.axivasichem.com

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