The date show were derived from a FRAP experiment on a transmembrane .pdf
The date show were derived from a FRAP experiment on a transmembrane protein incorporated in vitro into liposomes made from a single, typical phospholipid species. The experiment was performed at 4 degree C. What is FRAP an acronym for? In one sentence or less, predict and explain how and why the results for this protein will change if the experiment was conducted at 37 degree C. Also, draw a solid curve on the figure above showing how the data would look. If you then kept the temperature at 37 degree C but incorporated cholesterol into your liposomes, what will happen? Predict and explain in one sentence or less, and draw a dashed curve on the figure showing how the data for this protein will change. Now, instead of liposomes you are monitoring the diffusion of this protein in the plasma membrane of living cells at 37 degree C. To your surprise the data look completely different (see Fig. B on right)! In 1-2 sentences provide a biological (not methodological) explanation. Solution 1. FRAP is an acronymn for Fluorescence recovery after photobleaching. FRAP is a method for determining the kinetics of diffusion through tissue or cells. It is capable of quantifying the two dimensional lateral diffusion of a molecularly thin film containing fluorescently labeled probes, or to examine single cells. This technique is very useful in biological studies of cell membrane diffusion and protein binding..
The date show were derived from a FRAP experiment on a transmembrane .pdf
The date show were derived from a FRAP experiment on a transmembrane protein incorporated in vitro into liposomes made from a single, typical phospholipid species. The experiment was performed at 4 degree C. What is FRAP an acronym for? In one sentence or less, predict and explain how and why the results for this protein will change if the experiment was conducted at 37 degree C. Also, draw a solid curve on the figure above showing how the data would look. If you then kept the temperature at 37 degree C but incorporated cholesterol into your liposomes, what will happen? Predict and explain in one sentence or less, and draw a dashed curve on the figure showing how the data for this protein will change. Now, instead of liposomes you are monitoring the diffusion of this protein in the plasma membrane of living cells at 37 degree C. To your surprise the data look completely different (see Fig. B on right)! In 1-2 sentences provide a biological (not methodological) explanation. Solution 1. FRAP is an acronymn for Fluorescence recovery after photobleaching. FRAP is a method for determining the kinetics of diffusion through tissue or cells. It is capable of quantifying the two dimensional lateral diffusion of a molecularly thin film containing fluorescently labeled probes, or to examine single cells. This technique is very useful in biological studies of cell membrane diffusion and protein binding..
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The date show were derived from a FRAP experiment on a transmembrane .pdf
- 1. The date show were derived from a FRAP experiment on a transmembrane protein incorporated in vitro into liposomes made from a single, typical phospholipid species. The experiment was performed at 4 degree C. What is FRAP an acronym for? In one sentence or less, predict and explain how and why the results for this protein will change if the experiment was conducted at 37 degree C. Also, draw a solid curve on the figure above showing how the data would look. If you then kept the temperature at 37 degree C but incorporated cholesterol into your liposomes, what will happen? Predict and explain in one sentence or less, and draw a dashed curve on the figure showing how the data for this protein will change. Now, instead of liposomes you are monitoring the diffusion of this protein in the plasma membrane of living cells at 37 degree C. To your surprise the data look completely different (see Fig. B on right)! In 1-2 sentences provide a biological (not methodological) explanation. Solution 1. FRAP is an acronymn for Fluorescence recovery after photobleaching. FRAP is a method for determining the kinetics of diffusion through tissue or cells. It is capable of quantifying the two dimensional lateral diffusion of a molecularly thin film containing fluorescently labeled probes, or to examine single cells. This technique is very useful in biological studies of cell membrane diffusion and protein binding.