Title: proteomics scope and its importance this lect will cover that what is proteomics? why it is important and also this helps us in understanding biological processes and advancing the field of system biology. for identification of proteins in normal and diseadse condition etc. this lecture will help all the students in field of biotechnology, molecular biology and field of proteomics students,

1. A N I Q A A T T A
M B G , M P H I L 2 N D S E M E S T E R
PROTEOMICS SCOPE AND
ITS IMPOTANCE

2. CENTRAL DOGMA OF MOLECULAR
BIOLOGY
The central dogma of molecular biology describes the
two-step process,
1. Transcription
2. Translation
By which the information in genes flows into
proteins: DNA → RNA → protein

3. CENTRAL DOGMA

4. HISTORY OF PROTEOMICS
 Term ‘protein’ was initially introduced in 1938 by
swedish chemist jons jacob.
 He wanted to describe a particular class of
macromolecule, made up linear chains of amino acid.
 Protein study begins in 1975 with the introduction of
2Dgel and mapping from E.coli.
 The term proteome and proteomics was coined in
early in1990s by mark wilkon.

5. PROTEOME
 Contrast to genome
-the genome is constant for one cell and identical for all
cells of an organism, and does not change very much within
a species
-The proteome is very dynamic with time and in
response to external factors, and differ substantially
between cell types.

6. PROTEOME
 Variable
- in different cell and tissue types in same organism
- in different growth and developmental stages of
organism.
 Dynamic
- Disease state
- Drug challenge
- Growth condition
- Stress

7. PROTEOMICS
Proteomics: is the study of total protein complements,
proteomes e.g. from a given tissue or cell type.
Definitions:
- Classical: restricted to large scale analysis of
gene products involving only proteins.
- Inclusive: aims to identify all the proteins in a
cell or organism including any posttranslationally
modified forms, as well as their cellular localization.
Function and interactions.

8. WHY PROTEOMICS
 Protein function
 Protein post translational modification
 Protein localization and compartmentalization
 Protein protein interactions
 Protein expression studies

9. KINDS OF PROTEOMICS
 Protein expression
- Quantitative study of protein expression between samples
that differ by some variable
 Structural proteomics
- Goal is to map out the 3-D structure of proteins and protein
complexes
 Functional proteomics
-To study protein protein interaction, 3D structures, cellular
localization and PTMS in order to understand the
physiological function of the whole set of proteome

10. LARGE SCALE PROTEIN ANALYSIS
 2D protein gel
 Yeast two hybrid
 Rosetta stone approach
 Pathways

11. THE SCOPE OF PROTEOMICS
 Humans have 20,000 to 25,000 basic protein types.
 Proteins can be studied in various contexts, including sequence,
structure, interactions, expression, localization and modification.
 Proteomics is divided into several major but overlapping
branches, that embarce these different contexts:
- Sequence or structural proteomics
- Expression proteomics
- Interaction proteomics
- Functional proteomics

13. IMPORTANCE OF PROTEOMICS

14. SUMMARY