1. © European Union, 2002-2013 | http://europass.cedefop.europa.eu Page 1 / 3
Amarish Kumar Sharma
Norrebrogade 51, st 0207, Copenhagen 2200, Denmark.
0551-650 5550 (India)
+45 71 36 92 93 (Copenhagen)
amarish_03@yahoo.com
amarish.sharma
Danish Work Permit ( Person ID: 2913541)
Sex : Male | Date of birth: 04/07/1980 | Nationality : Indian
WORK EXPERIENCE
PREFERRED JOB
Research Scientist/Research Associate/Laboratory Technician.
August 2010- October 2014
Assistant Professor
Lovely Professional University, Punjab, INDIA | www.lpu.in
▪ Research & Development – Metabolic Disorder (Diabetes), Water Bioremediation, Food Bio- preservation.
▪ University teaching of subjects namely Stem Cell Technology & Animal Biotechnology, Administrative responsibilities(Pedagogy development, Training, workshop & seminar organization)
Research & Development | University Education
September’2009-April’2010
Research Scientist
Biocon Ltd , Bangalore, INDIA | www.biocon.com
▪ Assay development & Standardization for efficacy study (Biosimilars/Bioequivalence studies).
▪ High-throughput screening (HTS) of NCE’s (Natural/Synthetic therapeutic molecules) using mammalian cell lines (L6, C212, H9C2, 3T3-L1, 3T3-A31, HepG2).
▪ Projects handled:
 Standardization of the diabetic in-vitro assay model which deals with the comparative study of the efficacy of different Insulin analogues (Bio-similar), through assessing the percentage glucose drop using 3T3-L1 cell line.
 Standardization of Mitogenic or cell proliferation assay model for the investigation of mitogenic property of different Insulin analogues (Bio-similar) on induced diabetic model (hyperglycaemic condition) using 3T3-L1 cell line.
Research & Development | Drug discovery Program

2. EDUCATION AND TRAINING
March’ 2008-May’2009
Research Scientist
Connexios Life Sciences, Bangalore, INDIA | www.connexios.com
▪ Assay development & Standardization for efficacy (Biosimilars/Bioequivalence studies)
▪ Toxicity studies (Toxicogenomics,ADME/Tox), its standardization/optimization.
▪ High-throughput screening (HTS) of NCE’s(Natural/Synthetic therapeutic molecules) using mammalian cell lines(C2C12,H9C2,3T3-L1,HepG2,HEK293,CHO-K1).
▪ Projects handled:
 Investigated the effect of Hepatocyte Nuclear Factor-4# phosphorylation and its transactivation and assessing AMPK activation in Hepatocytes on Type 2 Diabetes assay model.
 Assessed the effect of cationic amphiphilic drugs induced Phospholipidosis and their in-vitro high throughput screening(HTS) in CHO-K1 cell line using the fluorescent-labeled phospholipid probe.
 Standardized the bio-assay for the analysis of Nephrotoxicity (ADME/Tox) in HEK-293 cell lines and in-vitro screening of the NCE’S(New Chemical Entities) on induced Type 2 Diabetic assay model.
 Investigated the relationship between IMTG (Intramuscular triglycerides) and Insulin resistance in Obese Type 2 Diabetic in-vitro model on C2C12 cell line followed by high throughput screening of new chemical entities for its respective efficacy.
 Investigated the effect of HSD11b1 mediated Endoplasmic Reticulum stress (ADME/Tox) on Type 2 Diabetic invitro model using C2C12 cell line.
 Studied the expression of CPT1A gene and ECHS1 gene which play a major role in accumulation of fat in the liver and is associated with insulin resistance and type 2 diabetes mellitus followed by high throughput in-vitro screening of new chemical entity for its efficacy.
Research & Development | Drug discovery program
August 2010- October 2014
Research Intern
Nicholas Piramal Research Centre, Mumbai, INDIA | www.piramal.com
▪ Completed project work entitled “Bioactivity guided fractionation and in-vitro screening of MHCO2 isolated from marine bacteria showing Anti-Diabetic Activity”.
▪ Assay development & Standardization for efficacy (Bio-similar / Bioequivalence studies) and high- throughput screening (HTS) of NCE’s(Natural/Synthetic therapeutic molecules) using mammalian cell lines(L6),Radio-isotope handling.
Research & Development | Drug discovery program
August 2006-December 2007
Master of Technology (M.Tech) in Biotechnology
Bundelkhand University, INDIA
 Medical Biotechnology, Animal Cell Culture, Bio-analytical techniques in Biochemistry, Fermentation Technology, Immunology, Molecular Biology and Biochemistry
July 2002 - July 2006
Bachelor of Technology (B.Tech) in Biotechnology
Bundelkhand University, INDIA
 Animal Biotechnology, Bioprocess Technology, Fermentation Technology, Immunology, Molecular Biology and Biochemistry, Biostatics.

3. © European Union, 2002-2013 | http://europass.cedefop.europa.eu Page 3 / 3
PERSONAL SKILLS
 Expertise in Animal Cell Culture;Cell line Maintenance& its Cryopreservation.
 Cell line characterization and validation.
 Assay development for efficacy (Biosimilars/Bioequivalence studies).
 Toxicity studies (Toxicogenomics,ADME/Tox).
 High-throughput screening(HTS).
 RNA isolation, cDNA preparation and quantitative RT-PCR.
 SDS-PAGE, Western blotting, and fluorescent imaging techniques.
 Experience in working with radioisotopes.
 Handled In-vivo experiments (Islets isolation, Pancreas, Mouse model).
 Experience with data analysis and graphing software.
 Interpret and report data to the drug discovery teams.
 Prepare and maintain accurate laboratory records (SOPs).
 Experience in handling HPLC and Mass Spectrometry.
 TLC, Column Chromatography, End point Thermocycler, Agarose gel electrophoresis.
Mother tongue
Hindi
Other language(s)
UNDERSTANDING
SPEAKING
WRITING
Listening
Reading
Spoken interaction
Spoken production
English
C1/2
C1/2
C1/2
C1/2
C1/2
Replace with name of language certificate. Enter level if known.
Danish
A1/2
A1/2
A1/2
A1/2
A1/2
Replace with name of language certificate. Enter level if known.
Levels: A1/2: Basic user - B1/2: Independent user - C1/2 Proficient user
Common European Framework of Reference for Languages
Computer skills
▪ Good command of Microsoft Office™ tools
▪ Good command on statistical tools such as Graph pad prism & PLA, for analysing data.
Presentations/Conferences
Certifications
Memberships
 Poster Presentation- "Control of Cataract by increasing the activity of enzyme Polyol Dehydrogenase" : International Conference On Biomedical Research and Therapeutic Medicine, Bundelkhand University, India January 8, 2005;Authors: Amarish Sharma, Saurabh Verma
 Poster presentation on “ELISA” in All India Engineering Project Contest, organized by Institute Of Engineering & Technology, Bundelkhand University, Jhansi, INDIA on March 21st, 2005.
 Participated in National Sensitization Programme on Biotechnology held at D.D.U. Gorakhpur University, Gorakhpur, U.P, INDIA on 6th March, 2005.
 Workshop on Chromatography & Mass Spectrometry-Certificate of Attendance ,Indian Institute of Chromatography and Mass Spectrometry LLP License 100108 March 2014
 Summer Training at Post Graduate Department of Microbiology, King George Medical University,Lucknow-226003 License 1374/05 ; DOI: 07.09.05 September 2005
 Faculty Development Programme on "Stem Cell Technology”, Lovely Professional University License 3652 June 2013
 Faculty Development Programme on “Immunology and Immuno-technology, Lovely Professional University License 1615 July 2011
 MEMBER OF HELP AGE INTERNATIONAL CHARITABLE TRUST
 MEMBER OF INDIAN SOCIETY FOR RADIATION BIOLOGY