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Dna vaccines

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Dna vaccines

  1. 1. Medical Microbiology HS0804
  2. 2.  Purpose: induction of an immune response to achieve protection against an antigen to protect a future challenge or to initiate a reaction against already present antigent Method: Injection or oral uptake( e.g. Protein, toxin, attenuated pathogen etc.) together with adjuvant or carrier
  3. 3.  DNA vaccines are made of a modified form of an infectious organisms DNA microbe DNA( not human) is inserted into plasmid vector when injected into a person(intramuscular injection, intradermal saline injection) -leads to the expression of the genes within this modified, foreign DNA result, the persons immune system responds in both antibody and cell mediated
  4. 4.  when injected into a person -leads to the expression of the genes within this modified, foreign DNA result, the persons immune system responds in a protective manner Human cells take up some plasmids and express microbial DNA in the form of protein
  5. 5.  Gene gun: initiates responses by transfected or antigen bearing epidermal Langerhans cells that move in lymph from bombarded skin to the draining lymph nodes Intramuscular injection: the functional DNA appears to move as free through blood to spleen where professional APC initiate responses

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