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Person A has 10 mutations in the 20 bp region where the forward primer binds during amplification
of D1S80. These mutations change the DNA sequence of that region significantly. Which of the
following is the likely outcome during PCR? (Select ail that apply) The reverse primer will bind
provided that there are no mutations in the region where it anneals. There will be no PCR product
or very low level of PCR product The forward primer will not anneal or will anneal at a very low
level There will be ample amount of PCR product but only from the reverse strandt

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Person A has 10 mutations in the 20 bp region where the forw.pdf

  • 1. Person A has 10 mutations in the 20 bp region where the forward primer binds during amplification of D1S80. These mutations change the DNA sequence of that region significantly. Which of the following is the likely outcome during PCR? (Select ail that apply) The reverse primer will bind provided that there are no mutations in the region where it anneals. There will be no PCR product or very low level of PCR product The forward primer will not anneal or will anneal at a very low level There will be ample amount of PCR product but only from the reverse strandt