practical

1. The Rawalpindi Medical University
FOUNDATION MODULE
SKILL LAB /Physiology PRACTICAL
Dr. Maryam Abbas Dated: 11-03-23

2. INTRODUCTION OF CENTRIFUGE
MACHINE
First Year Physiology Practical
2

3. The Rawalpindi Medical University
Motto Vision; The Dream/Tomorrow
• To impart evidence
based research oriented
medical education
• To provide best possible
patient care
• To inculcate the values
of mutual respect and
ethical practice of
medicine
3

4. LEARNING OBJECTIVES
• At the end of the session students should be able to
identify
• Centrifuge Machine
• its
• Understand the Concept of Hematocrit
4

5. Centrifuge machine and its
components
5

6. Micro Hematocrit Centrifuge machine
6

7. Points of identification
(centrifuge machine)
• This machine is identified by its different parts which
are as follows:
• Power
• Braker
• Timer
• Opening lid
• Rotor
7

8. Principle
• When anticoagulated whole blood is centrifuged,
the space occupied by the packed red blood cells is
defined as the hematocrit (HCT), and is expressed
as the percent of red blood cells in a volume of
whole blood.
8

9. Mechanism of Cenrtifuge
• A centrifuge works by using the principle of
sedimentation: Under the influence of gravitational
force (g-force), substances separate according to
their density.

10. Functions of Hematocrit
• Hematocrit is a blood test that measures how much
of a person's blood is made up of red blood cells.
This measurement depends on the number of and
size of the red blood cells. Blood transports oxygen
and nutrients to body tissues and returns waste and
carbon dioxide.
10

11. Questions related to centrifuge machine and
hematocrit
1. Write down the identification points of centrifuge
machine.
Key:
Machine is identified by its parts and specific shape .
Main parts are opening lid, rotor , braker ,power and
timer.
2. Define hematocrit and its normal value.
Key:
The ratio of the volume of packed red blood cells to the
total blood volume is called hematocrit. Its value is 45%
of the total volume of a blood sample. 11

12. MCQS
1. Mechanism of centrifugation is used for the measurement of:
a) Hematocrit*
b) Erythrocyte sedimentation rate
c) Red blood cell count
d) Total leukocyte count
e) Bleeding time
2. Following is not the part of micro hematocrit centrifugation
machine:
a) Power
b) Braker
c) Timer
d) Opening lid
e) Fine adjustment knob* 12

13. Learning Resources
• Practical physiology 1st year MBBS by Dr. Saqib
Sohail.
• Google images
13

14. • Steps to Access HEC Digital Library
1. Go to the website of HEC National Digital Library.
2. On Home Page, click on the INSTITUTES.
3. A page will appear showing the universities from Public and
Private Sector and other Institutes which have access to
HEC National Digital Library HNDL.
4. Select your desired Institute.
5. A page will appear showing the resources of the institution
6. Journals and Researches will appear
7. You can find a Journal by clicking on JOURNALS AND
DATABASE and enter a keyword to search for your desired
journal.
Link:https://www.topstudyworld.com/2020/05/access-
hec-digital-library.html?m=1
How To Access Digital Library
14

15. 15