1. TISSUE CULTURE
Prepared by
Dr. Vinay T.

2. Table of Contents
• Tissue Culture
• “Tissue culture is the technique of growing cells and tissues in an artificial
medium separate from the organism.”
• Table of Contents
• Explanation
• Types
• Steps
• Advantages
• Importance
• What is Tissue Culture?
• Tissue culture is a technique in which fragments of plants are cultured and
grown in a laboratory. Many times the organs are also used for tissue
culture. The media used for the growth of the culture is broth and agar.

3. Technique
• Tissue culture is a technique in which fragments of
plants are cultured and grown in a laboratory. Many
times the organs are also used for tissue culture. The
media used for the growth of the culture is broth and
agar.
• This technique is also known as micropropagation. It
has proved beneficial for the production of disease-
free plants and increase plant yield in developing
countries. It only requires a sterile workplace,
greenhouse, trained manpower, and a nursery.

4. Phases
• Initiation Phase
• At this stage, the tissue is initiated into the
culture. The tissue of interest is obtained,
introduced and sterilized to prevent the
process from any contamination.

5. Multiplication Phase
• Multiplication Phase
• In this stage, the sterilized explant is
introduced into the medium composed of
growth regulators and appropriate nutrients.
They are responsible for the multiplication of
cells. This undifferentiated mass of cells is
known as a callus.

6. Root Formation
• Root Formation
• The roots start forming. Plant growth
hormones are added to initiate the root
formation. Consequently, we obtain a
complete plantlets.

7. • Shoot Formation
• The plant growth hormones for the formation
of shoot are added and the growth is
observed for a week.

8. Acclimatization
• Acclimatization
• When the plant starts developing, it is
transferred to the greenhouse to develop
under controlled environmental conditions. It
is finally transferred to the nurseries to grow
under natural environmental conditions.

9. • Advantages of Tissue Culture
• Following are the various advantages of tissue
culture technique:
• The plantlets are obtained in a very short time
with a small amount of plant tissue.
• The new plants produced are disease-free.

10. • The plants can be grown throughout the year,
irrespective of the season.
• A large space is not required to grow plants by
tissue culture technique.
• The production of new varieties in the market
place speeds up.

11. Importance of Tissue Culture
• This technique is being used for the production of
ornamental plants such as dahlia, chrysanthemum,
orchids, etc.
• Importance of Tissue Culture
• Tissue culture is very important in biology due to its
wide range of applications.
• Both plant and animal tissues can be used for culturing.
For eg., animal tissue culture helps in preserving an
organ or tissue.

12. • Plant tissue culture may be used for genetic
modification of a plant or simply increase its
yield. the cells of the plants can be genetically
altered to produce plants with desirable
characteristics.
• This technique utilizes the plant’s ability to
rejuvenate the tissues rapidly. It produces
exact copies of itself known as clones.

13. • It is a technique of quickly producing plants
without any tubers, seeds or bulbs.
• It also helps in the conservation of plant
biodiversity by the production of endangered
plants.
• For more information on Tissue Culture and
related topics, keep visiting BYJU’S website or
download BYJU’S app for further reference.

14. • Thank You!