3. Rieske Proteins
• The Rieske protein is found in complex III
(cytochrome bc1 complex) as part of the
electron transport chain.
• This complex oxidizes ubiquinol to
ubiquinone and transports the electrons
through the complex into the Fe-S cluster in
Reiske.
http://www.scienceprofonline.org/metabolism/electron-transport-chain-cellular-respiration.html
http://en.wikipedia.org/wiki/Coenzyme_Q_%E2%80%93_cytochrome_c_reductase
4. H134C mutant & MitoNEET
• The Rieske protein Fe-S cluster has a 2-His 2-
Cys ligation environment.
• H134C mutant-the ligation structure is
altered to have a one histidine and three
cysteines.
• This ligation environment is similar to that of
MitoNEET, a known diabetes drug activator.
• Results from these experiments will be used
to compare to that of Mitoneet to better
understand the differences with this ligation
environment.
Rieske
MitoNEET
PDB ID 3REE
14. Conclusions
• H134C pKa of protein is 9.86.
• Crystal structure confirms the 3Cys 1 His
Structure
• Protein is modified by DEPC and not reduced
within a 90 minute reaction.
• DEPC modification may reverses over longer
times, indicating a labile adduct in this 3 Cys, 1
His ligation environment.