(WIDAL TEST)
Presented by:-
Mr. ASHISH

SPECIFIC OBJECTIVE
 DEFINITION OF WIDAL TEST
 TYPE OF WIDAL TEST

DEFINITION OF WIDAL TEST
 Widal test is a type of agglutination test which is used to
typhoid fever ( enteric fever)
 The widal test detects antibodies against O( somatic surface
antigen) or H (flagella antigen of the suspected organism
)antigens.
 Type of technique is use direct agglutination .
 Widal test was firstly discovered by Georges fernand widal.

SALMONELLA ANTIGEN
They are two type of salmonella bacteria.
Salmonella typhi
(s.paratyphi)
Antigen O (TO)
Antigen H (TH)
Salmonella typhi
(s.typhi)
Antigen AH
Antigen BH

PROCEDURE OF WIDAL TEST
1.SLID AGGLUTINATION TEST:
1.REQUIREMENTS:-
Widal
Dropper
Mixture
2.SAMPLE-patient serum
3. REAGENT-
O-antigen AH-antigen
H-antigen BH-antigen

PRICIPAL OF WIDAL TEST
 Widal test is type of agglutination test.
 Patient serum mix antigen.
 If any antibody present in patient serum then agglutination
occur .
 If antibody not present then agglutination not occur.
 Test positive-(agglutination present)
 Test negative-(agglutination absent)

PROCEDURE OF WIDAL TEST
 Take clean and dry widal slide.
 Now add 1-1 drop serum in each circle.
 Mix all antigen in each circle O,H,AH,BH.
 Mix of thoroughly with aid of the applicator stick and rotate
the slide gently.
 Now see the agglutination.
RESULT- agglutination was observed with in a 1min
positive& negative

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PROCEDURE OF DILUTION TEST
TUBE
 For the preparation of dilution add 0.9 normal saline and
0.1ml patient serum in first tube and other tube add 0.5 ml
normal saline.

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THANK YOU