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Exploring
The
Unknown
Tree Bark Sample
FROM THE PHILIPPINES, AS A NATURAL PRODUCT
Vanessa Ford
Honors Component for SCI 28...
Objective
Unknown Tree Bark Sample from Philippines
Medicinal or Analgesic Properties?
The Aeta People – An indigenous tribe of the Philippines
Background
Background
Pterocarpus Indicus Willd,
Narra Tree
Moringa Oleifera
Malunggay Tree
Trees in the Philippines
with known medic...
Tea of the
Indigenous Philippine
Tribes
Introduction
Experimental Methods
Boiling Bark
Concentrating
Sample
Crystallization
Experimental Methods
Vacuum Filtering System
Experimental Methods
Resulting Products
Experimental Methods
Sample A
(Filtered Liquid)
Sample B
(Filtered Solid)
Sample C
(Filtered Solid,
Re-...
Fractional Distillation
Experimental Methods
of Sample A (Filtered Liquid)
Experimental Methods
Products of Sample A
Distillate Liquid Remaining solution
After distillation
Vacuum filtered Vacuum f...
Experimental Methods
& Results
Thin Layer Chromatography
(TLC)
Liquid Samples (A, C, D, and E)
Sample C (the initial solid...
Melting Point Apparatus
Melting Points of Solid Samples (B, F, and G)
Experimental Methods
& Results
Melting Points of 2 o...
WHOLE PRODUCTS EXTRACTION
Future Direction
V.S
Future Direction
Human Digestive System
Thank You
References
• Shriner, Ralph L., Christine K. F. Hermann, Terence C. Moril, David Y.
Curtin, and Reynold C. Fuson. The Syst...
References
• S, Wang, Ding S, Zhang X, and Zhang L. "A New Medicinal Plant
Pterocarpus Indicus." NCBI (1997). NCBI. Web. 1...
Media References
• Bark background, http://blog.ppnf.org/vitamin-c-what-it-does-where-
to-get-it-how-to-supplement-it/
• A...
Media References
• Tea in cup,
http://spiritscienceactivenation.net/Oz/phpBB3/viewtopic.php?f=49&t
=182
• Cutting of Bark,...
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  1. 1. Exploring The Unknown Tree Bark Sample FROM THE PHILIPPINES, AS A NATURAL PRODUCT Vanessa Ford Honors Component for SCI 281 Prof. Kimberly Stieglitz Dec. 15, 2014
  2. 2. Objective Unknown Tree Bark Sample from Philippines Medicinal or Analgesic Properties?
  3. 3. The Aeta People – An indigenous tribe of the Philippines Background
  4. 4. Background Pterocarpus Indicus Willd, Narra Tree Moringa Oleifera Malunggay Tree Trees in the Philippines with known medicinal properties
  5. 5. Tea of the Indigenous Philippine Tribes Introduction
  6. 6. Experimental Methods Boiling Bark
  7. 7. Concentrating Sample Crystallization Experimental Methods
  8. 8. Vacuum Filtering System Experimental Methods
  9. 9. Resulting Products Experimental Methods Sample A (Filtered Liquid) Sample B (Filtered Solid) Sample C (Filtered Solid, Re-suspended in water)
  10. 10. Fractional Distillation Experimental Methods of Sample A (Filtered Liquid)
  11. 11. Experimental Methods Products of Sample A Distillate Liquid Remaining solution After distillation Vacuum filtered Vacuum filtered Sample D (liquid) Sample F (solid) Sample E (liquid) Sample G (solid)
  12. 12. Experimental Methods & Results Thin Layer Chromatography (TLC) Liquid Samples (A, C, D, and E) Sample C (the initial solid, re-suspended in water) was the only one to test positive for an active compound
  13. 13. Melting Point Apparatus Melting Points of Solid Samples (B, F, and G) Experimental Methods & Results Melting Points of 2 out of 3 solids were determined to be above 150 °C Further testing needed in order to determine exact melting points Closer to characterization based on comparison to literature
  14. 14. WHOLE PRODUCTS EXTRACTION Future Direction V.S
  15. 15. Future Direction Human Digestive System
  16. 16. Thank You
  17. 17. References • Shriner, Ralph L., Christine K. F. Hermann, Terence C. Moril, David Y. Curtin, and Reynold C. Fuson. The Systemic Identification of Organic Compounds. 8th ed. Hoboken, NJ: Johnson Wiley & Sons, 2004. Print. • Seager, Spencer L., and Michael R. Slabaugh. Chemistry for Today: General, Organic, and BioChemistry. 8th ed. Belmont, CA: Mary Finch, /Cole, Cengage Learning, 2011. Print. • Seager, Spencer L., and Michael R. Slabaugh. Safety-Scale Laboratory Experiments for Chemistry for Today. 8th ed. Belmont, CA: Mary Finch, /Cole, Cengage Learning, 2011. Print. • Sasidharan, S., Y. Chen, and L. Yoga Latha. "Extraction, Isolation and Characterization of Bioactive Compounds from Plants' Extracts." African Journal of Traditional, Complementary, and Alternative Medicines (2010). NCBI. African Networks on Ethnomedicines. Web. 15 Nov. 2014. <http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218439/>.
  18. 18. References • S, Wang, Ding S, Zhang X, and Zhang L. "A New Medicinal Plant Pterocarpus Indicus." NCBI (1997). NCBI. Web. 15 Nov. 2014. http://www.ncbi.nlm.nih.gov/pubmed/12572429 • "A Bioactive Polyphenolic Constituent in the Bark of Pterocarpus Indicus, Willd. I. Isolation and Characterization.” CiNii. Web. <http://ci.nii.ac.jp/naid/130000025769/>. • Mbikay, Majamba. "Therapeutic Potential of Moringa Oleifera Leaves in Chronic Hyperglycemia and Dyslipidemia: A Review." Frontiers in Pharmacology (2012). Web. 25 Nov. 2014. • Fahey, Jed W. "Moringa Oleifera: A Review of the Medical Evidence for Its Nutritional, Therapeutic, and Prophylactic Properties. Part 1." Tree For Life Journal (2005). Trees for Life Journal. Web. 20 Nov. 2014. <http://www.tfljournal.org/article.php/20051201124931586>.
  19. 19. Media References • Bark background, http://blog.ppnf.org/vitamin-c-what-it-does-where- to-get-it-how-to-supplement-it/ • Aeta Family, http://kwekudee- tripdownmemorylane.blogspot.com/2012/10/aeta-people-one-of-first- natives-of.html • Bark pieces, http://www.herbmuseum.ca/content/why-pau-darco-so- effective • Cutting of Bark, http://home-remedies.wonderhowto.com/how- to/make-aspirin-from-willow-tree-0142525/
  20. 20. Media References • Tea in cup, http://spiritscienceactivenation.net/Oz/phpBB3/viewtopic.php?f=49&t =182 • Cutting of Bark, http://home-remedies.wonderhowto.com/how- to/make-aspirin-from-willow-tree-0142525/ • Narra Tree, plants.swtexture.com/2009_01_archive.html • Moringa Tree, fyi09.wordpress.com/tag/vegetable • Question Mark, icons.mysiteway.com/legacy-icon/114653-magic- marker-icon-alhpanumeric-question-mark1-ps/

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