Vandana Devesh Sharma

applications advantage instrumentation micro- volume sampling valves) septum- less injection stop flow (septum injectors 3. sample injection system pressure pump) pneumatic or constant- pump reciprocating syringe pump en 2. pumping system (screw- driv er 1.solvent reservoir and degass theory hplc- introduction procedure gel filtration tech sample 7.detector 6. components seperation from 5. effect of flow rate 4. choice of mobile phase 3. column parameters 2. sample size & concentration instrumentation- a. stationary molecular- sieve chromatograph size exclusion gel filtration/ gel exclusion gel permeation chromatography molecules. separating different sized type partition chromatography instrumentation applications introduction theory affinity chromatography- 4. detectors- photomultiplier matched quartz cuvettes) quartz cuvettes glass cuvettes sample cells gratings b.monochromators- prisms visible fluorescent radiation the secondary filter transmits the uv radiation while the primary filter- selects filters or dye containing wratten filters- made up of the glass detectors and polarisers filters sample cells/cuvettes light source monochromators / (single beam and double beam ) flourimetry- instrumentation instrumentation applications quenching fluorescence factors affecting conversions internal and external stages doublet and triplet electronic principle concept of singlet radiation intereference 2. chemical 3 1.temp of flame error/interference disadvantage readout device detectors-photo detector grating monochromator) (prism monochromators nebulizer instrumetation-source of flame flame emission spectroscopy atomic emission spectrometry interferences principle and applications of potentiometric titration methods to determine end point electrode) (metal electrodes and glass indicator electrodes calomel electrode) silver chloride electrode and of reference (standard hydroge construction and working electrochemical cell potentiometry instrumentaion polarography interfere aas instrumentation atomic absorption spectroscopy types conductometric titrations