1. Next Generation Sequencing
Clinical Examples 2.0In this tutorial we’re going to briefly go through some of the Clinical applications of Next Generation Sequencing and how it can be useful in determining inheritance patterns, distinguishing diagnosis between patients, and assessing the risks of an illness reoccurring.
2.
3.
4. Cancer
• Bladder Cancer
– T1NP: T1 non progressive cancer
• Patient may have multiple recurrences of the disease without
developing muscle-invasive tumors
– T1P: T1 progressive cancer
• Patient’s disease develops into muscle invasive tumors and
becomes metastatic.
• Once metastatic, cancer survival is around 5.4% at 5 years
5. Works Cited
DeFrancesco, Chris. "News Release." Partnership to Deliver Gains in Medical, Dental
Research. N.p., 28 Sept. 2009. Web. 30 June 2014.
<http://today.uchc.edu/newsreleases/2009/sep09/partnership.html>.
Gonzalez, Kelly. "Making Sense of NextGen Sequencing." Ambry Genetics. N.p., n.d.
Web. 27 May 2014.
<http://www.ambrygen.com/sites/default/files/pdfs/NERRG_4-10-
12_Making_Sense_of_NetGen_Sequencing_KG(3).pdf>.
Schug, Jonathan. "Next Generation Sequencing Core." Next Generation Sequencing.
N.p., n.d. Web. 30 June 2014.
<http://www.med.upenn.edu/cores/NextGenerationSequencingCore.shtml>.
"University of Wisconsin–Madison Biotechnology Center." UWBC DNA Sequencing
Facility. N.p., n.d. Web. 30 June 2014.
<http://www.biotech.wisc.edu/services/dnaseq>.
Editor's Notes
In this tutorial we’re going to briefly go through some of the Clinical applications of Next Generation Sequencing and how it can be useful in determining inheritance patterns, distinguishing diagnosis between patients, and assessing the risks of an illness reoccurring
Works Cited: accessed May 22 2014
http://srxa.files.wordpress.com/2013/10/neurodegenerative-disease.jpg
http://srxawordonhealth.com/2013/10/
One way that RNA Seq has proven to be useful as a diagnostic tool is in diagnosing certain disorders such as neurological disorders like Schizophrenia, Alzheimer's Disease, Parkinson’s Disease, and Huntington’s Disease.
Works Cited:
Accessed: May 22nd
http://www.ploscompbiol.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pcbi.1003517.g006&representation=PNG_L
Accessed: May 20th 2014
Soreq, Lilach, et al. "Long Non-Coding RNA And Alternative Splicing Modulations In Parkinson's Leukocytes Identified By RNA Sequencing." Plos Computational Biology 10.3 (2014): 1-22. Academic Search Premier. Web. 20 May 2014.
Leukocyte-Expressed protein coding transcripts, as well as non-coding areas of long non coding RNAs in Parkinson’s Disease patient have been characterized by RNA-Seq which helped in detecting blood based biomarkers on WBC.
