Atomic Absorption spectrometer is an instrument used for quantitative analysis of most of the metals in nano grams. This is highly sensitive technique used for analysis.

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Atomic Absorption SSppeeccttrroossccooppyy
（（ AAAASS))
APPLICATIONS
By:
Tapeshwar Bhardwaj

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AAttoommiicc AAbbssoorrppttiioonn
SSppeeccttrroossccooppyy
（（ AAAASS ））
· IInnttrroodduuccttiioonn
· IInnssttrruummeennttaattiioonn
· AApppplliiccaattiioonnss

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Innttrroodduuccttiioonn
What is AAS ?
Atomic Absorption Spectroscopy is
a quantitative method of analysis
that is applicable to many metals
and a few elements.

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What is AAS ?
Introduced in 1955
By Alan Walsh in Australia
First commercial AA Spectrometer
was introduced in 1955.
Alan Walsh 1916-1998

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What is AAS ?
• An atomic absorption spectrophotometer
consists of a light source, a sample
compartment and a detector.
SSaammppllee
CCoommppaarrttmmeenntt
LLiigghhtt SSoouurrccee DDeetteeccttoorr

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IInnssttrruummeennttaattiioonn
Line
source Atomization
Monochromator Detector
Nebulizer Read-out
Schematic diagram of a flame
spectrophotomer

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HHoollllooww CCaatthhooddee LLaammpp ((HHCCLL))
Cathode--- in the form of a cylinder,
made of the element being studied in
tAhneo fdlaem--e-tungsten

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A hollow cathode lamp ffoorr AAlluummiinnuumm ((AAll))

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SpectrAA - AAS
HCL
Motorized
Mirror

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Sample Atomization Techniques
Flame Atomization
Electro-Thermal Atomization/
Graphite Furnace
Hydride Atomization
Cold-Vapor Atomization

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Common fuels and oxidants used in flame
spectroscopy

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Atomic Absorption
Spectrophotometer

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Sensitivity and Detection limit (ng/mL)

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APPLICATIONS
• ENVIRONMENTAL SCIENCE
• FOOD TECHNOLOGY
•• PHARMACEUTICALS
• PETROCHEMICALS
•• GEOCHEMICAL/MINING
•• BIO-MONITORING
•• AGRICULTURE
• NANOMATERIALS
• PATHOLOGY

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IN FORENSIC SCIENCES
1. Determination of trace elements
2. Elemental profiles of biological samples.
3. Trace elements in artificial fibres.
4. Determination of the mode of poisoning.
5. Hair analysis for heavy metal poisons.
6. Determinations of ammunition manufacturers.
7. Discrimination of objects/Elements.

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Advantages and disadvantages
High sensitivity
[10-10g (flame), 10-14g (non-flame)]
Good accuracy
(Relative error 0.1 ~ 0.5 % )
High selectivity
Widely used
A resonance line source is required
for each element to be determined

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