1. 0th REVIEW
Phytochemical investigation and HR-LC MS Metabolite profiling of different
solvents extracts of Leucas linifolia
Team-5:
Arramali hema -190181033, Bartho – 190181013, Hellen - 190181003
IV B. Pharmacy
KONERU LAKSHMAIAH EDUCATION FOUNDATION
Under the Guidance
of
Dr. M.Narender
Mrs .N. Sri Lakshmi
Department of Pharmaceutical Chemistry
KL College of Pharmacy
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of Pharmacy
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2. OUTLINE
1. Introduction
2. Background with Literature Support
3. Motivation / Research Gaps
4. Hypothesis / Research Questions
5. Objectives
6. Methodology
1. Research Flow Diagram
2. Data Required
3. Analytical Tools Required
7. Proposed Plan
8. References
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3. INTRODUCTION
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.
• Medicinal plants are of great importance to researchers in the field of pharmacology as most
pharmaceutical industries depend on medicinal plant for their raw materials.
• Plants contain different phytochemicals, also known as secondary metabolites.
• Phytochemicals are useful in the treatment of certain disorders by their individual, additive, or synergic
actions to improve health. 1
• The development of new drugs starts with identification of active principles from the natural sources
• The screening of plant extracts is a new approach to find therapeutically active compounds in various
plant species. 2
4. INTRODUCTION
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.
• Phytochemicals such as flavonoids, tannins, saponins, alkaloids, and terpenoids have several biological
properties which include antioxidant, anti-inflammatory, anti-diarrhea, anti-ulcer, and anticancer
activities, among others.
Plant description
• Plant name: leucas linifolia
• Kingdom: Plantae
• Phylum: Tracheophyta
• Class: Mangoliopsida
• Order: Lamiales
• Family: Lamiaceae
• Genus: Leucas
6. BACKGROUND WITH LITERATURE SUPPORT
• Medicinal plants are used in traditional treatment to cure variety of diseases
• Modern plants has evolved from folk medicines that use plants as a source of drug
• Currently 80% of the world population depends on plant-derived drugs for their beneficial effects
to human because of its fewer side effects and the contain numerous compounds that have
significant pharmacological effects.
• Plants are rich in wide variety of secondary metabolites e.g. tannins, terpenoids, alkaloids and
flavonoids etc which have several biological properties.
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7. • Consequently screenings of active compounds from natural sources have shown efficient protection
and treatment roles against various diseases including cancer and Alzheimer's diseases. Thus
bioactive compounds from natural sources is deemed necessary.
• Phytochemical compounds also have an active role towards invention of novel semi-synthetic and
synthetic compounds
• The screening of plant extracts is a novel strategy to find therapeutically active compounds in many
plant species.
• Gas Spectroscopy (GC/MS) combined analytical techniques to find the presence of Phyto-chemical
compounds from the plant extract
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8. MOTIVATION / RESEARCH GAPS
• Extraction methods for the selective plant along with their phytochemical screening by using different
evaluation methods like GC-MS and HR LC-MS
• And to know different phytochemical constituents present in the selected plant for different organic
solvent extract.
• And to find constituents with drug like properties.
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9. HYPOTHESIS / RESEARCH QUESTIONS
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• Fascinating library of bioactive compounds with many pharmacological activities like antioxidant,
antimicrobial, anti-diabetic, antiparasitic, anti-tumour.
10. OBJECTIVES
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• The main objective of the present study was to investigate the phytochemical composition
of Leucas linifolia of different organic solvent extracts (Pet Ether, Ethyl acetate,
Chloroform, Methanol).
Objective 1
Objective 2
• Profiling of metabolites using the GCMS , HR-LCMS technique, the bioactive class of
compounds and its antioxidant and antidiabetic properties of different organic solvent
extracts (Pet Ether, Ethyl acetate, Chloroform, Methanol)
11. METHODOLOGY
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• The selected plant powder were defatted with petroleum ether (60-80°C) in Soxhlet apparatus
(continuous hot percolation process).
• after complete extraction (46 h), the solvent was removed and resulting liquid was dried using heating
plate at 50°C to get semisolid residue.
• After the extraction with petroleum ether, the same plant material was dried and further extracted with
ethyl acetate (50h) followed by methanol (75 h) until the extraction was complete.
• The methanolic, ethyl acetate extract was concentrated and dried using heating plate at 60°C to get
semisolid residue.
• Identification test for phytochemical screening
• Metabolite profiling by GC-MS and HRLC-MS
• Final conclusion
12. PROPOSED PLAN
1. Collection of plant materials (15 days)
2. Sieving and making cores powder (3 days)
3. Extraction (15 days)
4. Soxhlet extraction ( heating extraction)
5. Phytochemical test
6. TLC
7. HR-LC-MS( 30 days)
8. Thesis compilation (30 days)
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13. REFERENCES
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1. Chikezie PC, Ibegbulem CO, Mbagwu FN. Medicinal potentials and toxicity concerns of
bioactive principles. Med Aromat Plants. 2015;4(3):1-5.
2. Gopalakrishnan K, Udayakumar R. GC-MS Analysis of Phytocompounds of Leaf and Stem of
Marsilea quadrifolia (L.).
3. Starlin T, Prabha PS, Thayakumar BK, Gopalakrishnan VK. Screening and GC-MS profiling of
ethanolic extract of Tylophora pauciflora. Bioinformation. 2019;15(6):425.
4. . Ramakrishna H, Murthy SS, Divya R, MamathaRani DR, Panduranga Murthy G. Hydroxy
radical and DPPH scavenging activity of crude protein extract of Leucas linifolia: A folk
medicinal plant. Asian J Plant Sci Res. 2012;2(1):30-5.
5. Anarthe S, Chaudhari S. Hypoglycemic Activity of Leucas linifolia (Lamiaceae) Spreng.
Research J. Pharmacognosy and Phytochemistry. 2011 Feb 23;3(1):34-7.
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6. Manthena S, Arutla R, Mohan PC, Prusty B. Evaluation Of Antidiabetic And Antioxidant Activity
Of Root Extracts Of Herbal Mixture (Catharanthus Roseus, Leucas Linifolia). Experimental
animals. 2001;3:4.
7. Anarthe S, Raju MG, Chaudhari S. Neuropharmacological screening of Leucas linifolia
Spreng. Annals of Phytomedicine. 2012;1(2):56-61.
8. Rai VM, Pai VR, Kedilaya PH, Hegde S. Preliminary phytochemical screening of members of
Lamiaceae family: Leucas linifolia, Coleus aromaticus and Pogestemon patchouli.
International Journal of Pharmaceutical Science Review and Research. 2013;21(1):131-7.
9. Borah M, Das S. Antidiabetic, antihyperlipidaemic and antioxidant activities of leaves of leucas
linifolia spreng in streptozotocin induced diabetic rats.
15. THANK YOU
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16. INSTRUCTIONS
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