CRISPR-Cas is a genome editing technique derived from bacterial immune systems that allows for precise genomic modifications. The document discusses applications of CRISPR-Cas in plants, animals, and bacteria, including developing pest and disease resistant crops and livestock, modifying stem cells and embryos, targeting antibiotic resistant bacteria, and controlling gene expression.

1. Applications of CRISPR-CAS
System
-By Sarika Dengle
Roll no: 10487

2. INTRODUCTION
• CRISPR- Clustered Regularly Interspaced Short
Palindromic Repeats.
• Recently developed system.
• Genome editing technique derived from the
bacterial immune system.
• Precise genomic modifications.
• Gene sequences can be included from a range of
donors

3. Applications in Plants
• GE Crops:
• Better Pest Resistance
• Enhanced nutritional value
• Eg : White Button Mushrooms, Waxy Corn Hybrid.
• Crops with better root systems
• Haploid Plants
• Chemicals and Bio-materials

4. Applications In Animals
• Genetically engineer Embryonic Stem cells.
• Modify primate embryos
• Disease resistant Animals
• Eg: Pigs resistant to viral diseases, cattle resistant to
trypanosome parasites.
• Producing animals to give more yield.
• Designer babies and animals.

5. Applications in Bacteria
• CRISPR -CAS - Defense mechanism in 40% of
Bacterial species.
• This system on itself is lethal due to the NHEJ
pathway being ineffective.
• Targeting Antibiotic Resistant Bacteria
• Genome editing and Gene expression