1. Curriculum Vitae
Name: Sanjay Kumar Hirasker E-Mail: firstname.lastname@example.org
E.I. DuPont India Pvt. Ltd.: (Dec 2013 to Present) currently working as Research Associate on Yeast
metabolic engineering platform.
Evolva Biotech Pvt. Ltd based in Chennai, India (a subsidiary of Evolva Holdings, Swiss): (Nov 2007 to Nov
2013) worked in Cell and molecular biology department for 6 years as Research Scientist.
1 A dynamic professional with 8+ years of rich Industrial experience in Research & Development in a
synthetic biology with primary focus on basic research which discovers and provides innovative, high
value, sustainable ingredients – particularly for health, wellness and nutrition.
2 Cell biology: Development, optimization and screening of various cell based assays utilizing mammalian
3 Molecular biology: Thorough knowledge on cloning, expression of recombinant proteins in Yeast,
Bacteria and mammalian cell line culture.
4 Yeast metabolic engineering: Stable integration of heterologous pathways in Yeast and optimization
5 Analytical skills: Hands on experience in analyzing invitro and fermentation samples by HPLC to
determine metabolic flux (independent operations).
6 A rich experience in finding out missing enzymes of metabolic pathways which has economic significance
in the area of nutrition from pyrosequenced data.
7 An effective communicator with excellent relationship management skills and strong analytical, problem
solving & organizational abilities. Flexibility to work in any area which has great impact in improving lives
of common people.
Post-Graduation (M.Sc. Microbiology)
Osmania University, Hyderabad – Batch 2007. Secured 70.1% marks. (Secured 4th
place in the entire batch)
Graduation (B.Sc. - Microbiology / Zoology / Chemistry)
Osmania University, Hyderabad - 2005. Secured 68.9% marks. Stood 1st
(Awarded Gold medal)
Board of Intermediate Education 10+2 (Biology / Physics / Chemistry)
Govt. Junior College, Hyderabad - 2002. Secured 71.6% marks. (Secured 1st
place in the entire batch)
Mufeed-Ul-Anam Boys High School, Hyd- 2000. Scored 69.3% marks. (Secured 1st
place in the entire batch)
Technical Expertise –Industrial Experience
Research & Development: Cell and molecular biology:
Mammalian Cell line: Development of replicon model assay system for alpha-like virus class in
mammalian cell line (HEK-293) which has relevance with Dengue virus, Chikungunya virus and HCV
replication (+ve standed RNA virus).
Identification of small molecule based immunostimulators that activate Toll like receptor – Development
and high throughput screening of compounds through TLR8-SEAP Activation Assay in HeLa cell line.
Reporter assay development for screening compounds against NDM-1 enzyme target in mammalian cells.
2. Assay validation and screening of compounds on various target based mammalian in-vitro assay system
(HeLa, BHK-21, RAW 264.7) in 96 well formats.
Yeast metabolic engineering – Stable integration of heterogeneous pathways in Yeast to produce
commercially significant flavor, fragrance (F & F) and nutraceutical related molecules.
Development of knock-out strains of Yeast for pathway optimization.
Highly experienced in assay development, optimization & screening of small molecules on Yeast based in-
vitro assays like FRET signal detection systems for protein-protein interactions for anti-HIV and anti-
Ebola viral drug discovery programmes.
Worked on drug discovery programmes on Tuberculosis, anti-dandruff and melioidosis. Validation and
high-throughputisation of compound screening on Mycobacterium, Malassezia and Burkholderia strains
in different well formats.
Unknown enzyme identification and pulling out genes from pyro-sequenced data for mode of action
studies and functionality check.
Invitro transcription and translation of various proteins (enzymes) for testing the expression and
functionality of the genes.
Cloning, expression of various recombinant proteins in Bacteria, Yeast and Mammalian cells.
Wet lab Operations
Well experienced in handling various mammalian cell lines.
Various Yeast and mammalian cell based assay screening.
Testing inherent cyto-toxicity (by XTT assay methodology) on mammalian cell lines.
Well versed in all the molecular biology and cell culture techniques.
Study of genes involved in the plant resistance against pathogenesis.
Work experience in handling human pathogen (Mycobacterium tuberculosis) in one of the nation’s best
containment facility of BSL 3.
Follow all the standard international regulatory norms - ISO 9001-2008.
Electronic Data integration system of results obtained from all the experiments.
Ensuring compliance of various quality measures by maintaining requisite documentation / records.
Preparing periodic laboratory reports for information, compliance & corrective action.
1 Biochemical Techniques
Biochemical estimations, in-vitro Enzyme Assays, HPLC & Spectroscopic Techniques.
2 Molecular Biology & Immunological Techniques
USER and Infusion cloning methodology, In vivo (Conventional recombination) ligation methodology in
Yeast, In vitro transcription and translation-cell free assays, Electrophoresis, SDS-PAGE, Western blots,
Restriction digestion, Nucleic Acid Isolation, ELISA, DIG labelled probing and various Ag-Ab reaction
tests, Multiplex and RACE PCR, TA cloning, Cloning and expression studies of recombinant fusion protein
in E. coli.
Insilico genetic data analysis skills.
3 Microbiological/Cell culture Techniques
Bacterial/Yeast Transformation & all other microbiological and cell culture techniques, transfection
In-vitro anti-bacterial and anti-fungal assay in high throughput format (96 well and 384 well plate)
Bio analytical Techniques: Complete HPLC operation and analysis and interpretation of data.
3. Conferences/Meetings/Workshops attended
Poster presentation on “Development of on mammalian cell NDM-1 reporter assay development for
screening compounds against NDM-1 enzyme target” and got selected for Deakin University Ph.D.
Attended hands-on training on basics of lab scale bioreactor operations in Biozeen, Bangalore.
participated in a lecture workshop in modern biology ’’BIO-LOGIKS’’ from Molecules to processes under the
aegis of Indian Academy of Sciences, Bangalore and University of Hyderabad.
Poster presentation on ‘’Proteases’’ in annual lecture series ‘’Gyana Tarangini Vyakhyaana Mala’’
conducted by Bharatiya Vidya Bhavan’s College and our poster was awarded as best poster.
Field of Interest
Metabolic engineering in Yeast or any related organisms.
Drug discovery in the field of Infectious diseases especially the area of neglected diseases.
Has flexibility to work in any area which has great impact on improving lives of common people.
Date of Birth: 19th
of November 1984
Permanent Address: H.No: 8-12-96/R/52, Rajiv shetty Nagar, Karmanghat road, Hyderabad, A.P. PIN 500097
Linguistic Abilities: English / Hindi / Telugu / Marathi
References: Provided upon request (as separate attachment)
I hereby declare that the above information is true to the best of my knowledge.
Place: Hyderabad, India
Date: 25/01/2016 (SANJAY KUMAR)