1. Raymond P Damien
rpdamien@gmail.com
30 Glen Ave
Fitchburg, MA 01420
mobile: (978) 790-7742
Continued
SUMMARYOF EXPERIENCE
 HPLC/UPLC (Reverse Phase,SPE,GPC)
 Flash Column Chromatography (Purification)
 TLC (Thin Layer Chromatography)
 UV-Vis, Florescence (Florescamine), BCA,Micro BCA
 Gel and Capillary Electrophoresis
 DLS (Dynamic Light Scattering)
 Equilibrium Dialysis
 Cell line culture and maintenance
 Method Development, and Validation
 DoE (Design of Experiments)
 In vitro Release Studies and Data Interpretation
 Biologic and Drug Substance Encapsulation and Formulation
 Rheology
 Gel and Capillary Electrophoresis (SDS-PAGE and Agarose)
 DNA Extraction and PCR
 cGMP/GLP/GXP Environments
 Automation and System Integration
 SOP and Development Report writing
 Trained in MA RCRA and DOT hazardous materials and chemical hygiene requirements
EXPERIENCE
Selecta
Biosciences
Research Associate
Analytical Development
Watertown, MA,
July 2011 – February 2016
 Developed and improved sample preparation and analysis methods of complex Synthetic Vaccine
Particle components (Peptides and Proteins) by HPLC
 Developed and improved sample preparation and analysis methods of single strand synthetic DNA
(CpG) encapsulated nanoparticles by HPLC
 Experienced with using gradient reversed-phase separations,creation of HPLC methods, preparations
of mobile phases, and (Agilent 1100, 1260, and 1290 with Chemstation) HPLC maintenance
 Used DLS (Dynamic Light Scattering) to determine particle size and polydispersity
 Determined molecular weight by GPC (Gel Permeation Chromatography)
 Protein analysis by SDS-PAGE and antibody binding affinity through Equilibrium Dialysis
 Wrote SOP's and Reports to document test methods, procedures, OOT/OOS results, and technical
documents
 Trained in MA DOT regulations of 49 CFR 100-185, MA RCRA,and member of the Safety
Committee
 Served as analytical representative for 2 project teams
Ocular Therapeutix
(I-Therapeutix)
Research Associate
Research and Development/ Drug Delivery
Bedford, MA,
April 2008 – September 2010
 Developed methods of encapsulating drugs to be delivered Zero Order using biocompatible polymers
 Built MS Access database used to control chemical inventory, and synchronized it with electronic
versions of all MSDS sheets
 Provided initial preclinical work that changed company focus from stand-alone medical devices to
incorporate drug delivery
 Designed and developed hydrogel-based products using novel polymers and cross-linking
technologies
 Provided technical documents detailing the design and analysis to support design control

2. Raymond P Damien
rpdamien@gmail.com
Continued
 Developed correlational In Vivo/In Vitro studies to document efficacy of drug candidates and
determined the necessary response outputs
 Coordinated removal of lab waste per OSHA/DOT requirements and, trained and certified for 49 CFR
172 Subpart H
Xcellerex Research Associate
Process Development
Marlborough, MA,
September 2007 – April 2008
 Performed High Throughput Design of Experiments to design both Basal Growth Media and Feed
Media for a CHO cell line
 Cultured and maintained cell lines
 Automated methods for conducting a Design of Experiments (DOE) using robots from Tecan and
Caliper
 Developed robotic Hit Picking methods for the selection of clones
 Assisted in the development of a mini bioreactor
 Integrated multiple robots to reduce the need for constant human interface in GMP environments
Caliper Life
Sciences
Senior Product Test Scientist
Research and Development
Hopkinton, MA,
December 2006 – July 2007
 Developed qualification documents, IQ, OQ,and PQ for a robotic liquid handling system (Sciclone
ALH 3000, Affymetrix Genechip Array Station, and Zephyr) and a capillary electrophoresis system
(Lab Chip 90)
 Worked closely with R&D and Manufacturing to develop and troubleshoot new hardware and
software
 Provided expertise to assist in applications development, and technical support
 Developed test plans to document crucial changes necessary to successfully launch a product
 Performed statistical analyses regarding product reliability
 Researched and prepared a technical report on a high volume head with all associated arrays for a robotic
liquid handler
 Developed methods using UV-VIS detection and Fluorescence to determine a robotic liquid handler's
ability to perform serial dilutions
 Researched methods to effectively automate PCR of Affymetrix's Genome-Wide Human SNP
Anika
Therapeutics
Research Associate
Research and Development
Woburn,MA,
April 2004 – October 2006
 Performed purifications through Flash column chromatography
 Analyzed purity and concentration using HPLC (gradient and isocratic methods)
 Performed Design-of Experiments (DOE) on both the synthesis and purification of a novel cross
linker
 Experienced in using rheology to determine viscosity and enzymatic degradation of viscoelastic gels
 Performed TLC to determine proper specs for UV absorbance and detection criteria.
 Developed proper mobile phase components for chromatographic purification
 Developed Batch Records and Manufacturing Directions for multiple development products and
prepared technical reports based on original research and data
 Initiated and performed screening studies for the use of antioxidants in a formulation
 Developed and performed accelerated stability studies to determine the longevity of a product
Avecia
Biotechnology
QC Technician (Contract)
Quality Control
Milford, MA,
January 2004 – February 2004
 Responsible for gathering data on TOC and Conductivity for the qualification of a purified water
system according to cGMP and USP guidelines, and organizing data for qualification and validation
AstraZeneca Research Assistant
Cancer Bioscience R&D
Waltham, MA,
March 2002 - November 2002
 Performed DNA Sequencing by Capillary Electrophoresis
 Analyzed and reported sequencing data (contig alignments and description of any mutations)

3. Raymond P Damien
rpdamien@gmail.com
 Performed transforming of chemically competent cells for TOPO insertion cloning
 Performed PCR to amplify and ligate DNA and its specific primer
 Extracted DNA from cultures via spin column methods (Qiagen Mini Prep and Maxi Prep)
 Performed sub-cloning for site-directed mutagenesis reactions
Millennium
Pharmaceuticals
Research Assistant
Sequencing QA/QC
Cambridge, MA,
June 1999 – February 2000
 Performed DNA extraction via vacuum manifold methods
 Performed DNA Sequencing by gel and capillary electrophoresis
 Worked with robotic liquid handling devices (Tecan Genesis and a custom build from Cyberlab)
 Performed Single Stranded DNA reactions using the M13 bacteriophage, and Double Stranded
Reactions on plasmid DNA
 Designed Excel Macros for detailing raw data,and developd SOP’s for Documentation Control
EDUCATION
American International College
Springfield, MA
 Bachelors of Science degree in Biology, and Chemistry
 Awarded May 1999; Honors: GPA 3.3, Dean’s list, United Paper Worker’s Scholarship
COMPUTER SKILLS
UNIX Sequencher
Microsoft Office Suite Photoshop
DNA Star Maestro
Mantis Bug Tracker Merant Tracker
MS SQL iLink Pro
PDM Works Quick Test Professional
Visio Oracle
Agilent Chemstation Minitab
Waters Empower Shimadzu
OTHER
 Vice President of Operations for the Central Mass Cycling Club
 Fluent in French/Canadian French