emasculation and pollination technique in tobacco

1. EMASCULATION AND CROSSING TECHNIQUE IN TOBACCO

2. COMMON NAME :TOBACCO
BOTANICAL NAME: Nicotiana
tabacum
FAMILY :Solanaceae
ORIGIN :South America
CHROMOSOME
NUMBER : 2n=4x=48
POLLINATION : self pollination

3. Nicotiana tabacum (2n=48)
Nicotiana rustica (2n=48)
Cultivated species

4. Nicotiana undulata x Nicotiana paniculata
2n=24 2n=24
F1 (sterile)
Natural doubling
Nicotiana rustica
2n=2x=48
ORIGIN OF TOBACCO:

5. Floral parts:
1.Opened corolla tube showing
epipetalous stamen
2.Gynoecium with ovary in
longitudinal section
3.fruit(ovoid capsule)
Br, , ,K(5), C(5), A(5) ,G(2)

6. SYMMETRY
BRACTEATE
CALYX AND
COROLLA
ANDROECIUM
(5)
GYNOECIUM
Short 1
Long 4

7. BOTANICAL DESCRIPTION:
HABITAT:
N.tabacum - tropical
N.rustica - cooler climate
HABIT:
A weak stemmed herb, highly polymorphie
LEAVES:
N.tabaccum - simple,petiolate,cordate or semicordate shaped and evenly spaced
leaves
N.rustica - leave are thicker, dark green and have uneven surface. glandular hairs
on the surface of the leaves exerete gums which makes surface sticky.
Semicordate
(heart shaped)
glandular
hairs

8. INFLORESCENCE:
panicle, cymose type, pedicellate, bisexual.
CALYX:
five, cylindrical, pubescent, membranous with narrow pointed sepals with a
longer and odd sepal united.
COROLLA:
greenish yellow, pubescent oblong throat, tubular, broadly with contraction
near the mouth.
ANDROECIUM:
Epipetalous, stamens five , four long and one short.
GYNOECIUM:
ovary superior, style slender, stigma captate with axial placentation.
Cymose type
Contd….

9. FRUIT:
A capsule, elliptic, seeds very small, oval dusty brown.
SEED:
many, minute.
Oval dusty
brown
Contd….

10. ANTHESIS:
Chasmogamous nature
Pollens are viable for 24hrs
Stigma is receptive one day before and after flower opening

11. SELFING TECHNIQUE:
 Covering entire
inflorescence with paper
bag will ensure the
selfing.

12. CROSSING TECNIQUE:
1. EMASCULATION
Select the unopened flowers with pink color tip
and anthers are removed with fine pointed
forceps after tearing petal
2.CROSSING
Collect the pollen grains from matured flowers and dust it
on the emasculated flower
Bag the pollinated flower and then tag it

13. EMASCULATION

14. IMPORTANT VARIETIES:
Godawari,Swarna,Krishna,Thangam
Sona,Maragandam

15. THANK YOU