determination of extractives, acid soluble extractive, acid insoluble ash, water soluble extractive, ether soluble extractives, ash value.

1. DETERMINATION OF
EXTRACTIVES
determination of extractives TGAMC Ballari.
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PRESENTER
DR.PRIYANKA.PATIL
1ST YR P.G. SCHOLAR
Guide
Dr Ravi.R.Chavan
Dept of RS&BK
Asst. Professor
TGAMC, Ballari.
HOD
Dr. shankaragouda
Dept of RS & BK
Professor and HOD
TGAMC, Ballari

2. INTRODUCTION
• Extractives are the natural chemical products of biomass
that are capable of being extracted by some solvents.
• It comes under physical parameter of the drug evaluation.
• It helps to indicate the nature of chemical constituents
present in the drug.
determination of extractives TGAMC Ballari.
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3. TYPES
1.
• Alcohol
soluble
extractive
2.
• Water
soluble
extractive
3.
• Ether
soluble
extractive
determination of extractives TGAMC Ballari.
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4. Water soluble extractives
• Is applied for the drugs which contain water soluble constituents such as tannins,
sugars, plant acids and mucilage.
Alcohol soluble extractives
• For drugs which contain alcohol soluble constituents such as tannins, resins, and
alkaloids.
Ether soluble extractives
• For extraction of volatile oil, fixed oils and resins.
• volatile ether soluble extractive
• non volatile ether soluble extractive.
determination of extractives TGAMC Ballari.
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5. DETERMINATION
WATER SOLUBLE EXTRACTIVE VALUE
1) Macerate about 5gm of the accurately weighed coarse powder(air dried drug) with 100ml of
water in a 100ml volumetric flask for 24hrs.
2) Shake frequently for first 6 hrs.
3) Filter rapidly, through filter paper and evaporate 25ml of water extract (water bath) to dryness
in a tared flat bottomed shallow dish.
4) Dry the residue at 105 (muffle furnace) and weigh. Keep it in a desiccator.
5) Dry the extract to constant weight, finally calculate the % of W/W of water soluble extractive
value with reference to the air dried drug.
determination of extractives TGAMC Ballari.
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6. Weigh dry empty
crucible
Frequently stir 1st
6hrs
100ml water + 5gm
drug
Heat in muffle furnace Keep on water bath and evaporate add Filtrate to crucible
Filter rapidly Take 25ml filtrate
Dry in desiccator
Weigh crucible
again
determination of extractives TGAMC Ballari.
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7. CALCULATION
% of water soluble
extract
weight of residue
weight of drug
100
determination of extractives TGAMC Ballari.
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8. WATER SOLUBLE EXTRACTIVE VALUE.
S.No DRUG Water soluble extractive
(% W/W)
1. Aloe vera NLT 25.0
2. Linseed NLT 20.0
3. Senna leaves NLT 30.0
4. Ginger NLT 10.0
5. Glycyrrhiza NLT 20.0
determination of extractives TGAMC Ballari.
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9. DETERMINATION
ALCOHOL SOLUBLE EXTRACTIVE VALUE
1) Macerate about 5gm of the accurately weighed coarse powder(air dried drug) with
100ml of 90% alcohol in a 100ml volumetric flask for 24hrs
2) Shake frequently for first 6 hrs.
3) Filter rapidly, through filter paper and evaporate 25ml of alcohol extract (steam bath)
to dryness in a tared flat bottomed shallow dish.
4) Dry the residue at 105 (muffle furnace) and weigh. Keep it in a desiccator.
5) Dry the extract to constant weight, finally calculate the % of W/W of alcohol soluble
extractive value with reference to the air dried drug.
determination of extractives TGAMC Ballari.
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10. Weigh dry empty
crucible
Frequently shake
first 6hrs
100ml alcohol + 5gm
drug
Heat in muffle furnace Keep on water bath and evaporate add Filter to crucible
Filter rapidly Take 25ml filtrate
Dry in desiccator
Weigh crucible
again
determination of extractives TGAMC Ballari.
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11. ALCOHOL SOLUBLE EXTRACTIVE VALUE.
Sl. No. Drug. Alcohol soluble
Extractive % W/W.
1. Aloevera NLT 10.0
2. Benzoin NLT 90.0
3. Asafoetida NLT 50.0
4. Ginger NLT 04.0
5. Myrrh NLT 24.0
determination of extractives TGAMC Ballari.
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12. ETHER SOLUBLE EXTRACTIVE VALUE.
Follows same method as previous.
1. Volatile ether soluble extract - volatile oil, clove oil.
2. Non volatile ether soluble extract – resin, fixed oils coloring matter.
Sl. No Drug Non volatile ether
soluble extract
1. Capsicum NLT 12.0
2. Male fern NLT 01.0
3. Linseed NLT 25.0
determination of extractives TGAMC Ballari.
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13. SIGNIFICANCE
Extractive values are primarily useful for the determination of exhausted or adulterated drugs.
Helps to indicate the nature of chemical constituents present in the drug.
The extractive value of crude drug determines the quality as well as purity of the drug.
Useful in evaluation when the constituents of the drugs can not be readily estimated by any other means.
determination of extractives TGAMC Ballari.
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14. determination of extractives TGAMC Ballari.
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