NSTITUTEOF PHARMACEUTICAL EDUCATION AND RESEARCH BORGAON (MEGHE)
WARDHA - 442001

CONTENTS
INTRODUCTION
GOALS
VETERINARY CARE
QUARANTINE
SURVEILLANCE
PERSONAL HYGIENE
ANAESTHESIA & EUTHANASIA
MISCELLANEOUS

INTRODUCTION
COMMITTEE FOR THE PURPOSE OF CONTROL AND SUPERVISION OF EXPERIMENTS ON
ANIMALS (CPCSEA)
STATUTORY BODY FORMED BY THE ACT OF THE INDIAN PARLIAMENT UNDER THE PREVENTION
OF CRUELTY TO ANIMALS ACT, 1960
FORMED IN 1964
REVIVED IN 1998, UNDER THE COMMITTED CHAIRPERSONSHIP OF MENEKA GANDHI.

GOALS
TO PROMOTE THE HUMANE CARE OF ANIMALS USED IN RESEARCH.
TO AVOID UNNECESSARY PAIN BEFORE DURING AND AFTER EXPERIMENT.
TO PROVIDE SPECIFICATIONS THAT WILL ENHANCE ANIMALS WELL BEING AND QUALITY OF
RESEARCH.

VETERINARY CARE
ADEQUATE VETERINARY CARE MUST BE PROVIDED
AND IT IS THE RESPONSIBILITY OF A VETERINARIAN OR A PERSON WHO HAS TRAINING OR
EXPERIENCE IN LABORATORY ANIMAL SCIENCES AND MEDICINE.
OBSERVE REGULARLY FOR SIGN OF ILLNESS, INJURY, OR ABNORMAL BEHAVIOUR.

QUARANTINE
SEPARATION OF NEWLY RECEIVED ANIMALS FROM THOSE ALREADY IN THE FACILITY UNTIL THE
HEALTH AND POSSIBLY THE MICROBIAL STATUS OF NEWLY RECEIVED ANIMAL HAVE BEEN
DETERMINE.
A MINIMUM DURATION OF QUARANTINE FOR SMALL ANIMAL-1 WEEK AND FOR LARGER
ANIMAL-6 WEEK.

SURVEILLANCE, DIAGNOSIS, TREATMENT
AND CONTROL OF DISEASE
ALL ANIMAL SHOULD BE OBSERVED FOR SIGNS OF ILLNESS, INJURY, OR ABNORMAL
BEHAVIOUR BY ANIMAL HOUSE STAFF
ANIMALS THAT SHOW SIGNS OF A CONTAGIOUS DISEASE SHOULD BE ISOLATED FROM HEALTHY
ANIMALS IN THE COLONY.

PERSONAL HYGIENE
ANIMAL CARE STAFF MAINTAIN A HIGH STANDARD OF PERSONAL CLEANLINESS.
USE DISPOSABLE GEAR SUCH AS GLOVES, MASKS, HEAD COVERS, COATS, COVERALLS AND SHOE
COVERS.
PERSONNEL SHOULD NOT BE PERMITTED TO EAT, DRINK, SMOKE OR APPLY COSMETIC IN
ANIMAL ROOMS.

ANESTHESIA
IT MUST ALSO BE ENSURED THAT THE ANAESTHESIA IS GIVEN FOR THE FULL DURATION OF
EXPERIMENT AND AT NO STAGE THE ANIMAL IS CONSCIOUS TO PERCEIVE PAIN DURING THE
EXPERIMENT.
SEDATIVES, ANALGESICS AND ANAESTHETICS SHOULD BE USED TO CONTROL PAIN OR DISTRESS
UNDER EXPERIMENT.

EUTHANASIA
EUTHANASIA IS RESORTED TO EVENTS WHERE AN ANIMAL IS REQUIRED TO BE SACRIFICED ON
TERMINATION OF AN EXPERIMENT OR OTHERWISE FOR ETHICAL REASONS.
THE PROCEDURE SHOULD BE CARRIED OUT QUICKLY AND PAINLESSLY IN AN ATMOSPHERE FREE
FROM FEAR OR ANXIETY.

PHYSICAL FACILITIES
BUILDING MATERIALS - MOISTURE-PROOF, FIRE-RESISTANT, SEAMLESS MATERIALS ARE MOST
DESIRABLE PEST RESISTANCE.
CORRIDOR - WIDE ENOUGH TO FACILITATE THE MOVEMENT OF PERSONNEL AS WELL AS
EQUIPMENT AND SHOULD BE KEPT CLEAN.
UTILITIES - WATER LINES, DRAIN PIPES AND ELECTRICAL CONNECTION.
ANIMAL ROOM DOORS - RUST, VERMIN AND DUST PROOF AND PROVIDED WITH AN OBSERVATION
WINDOW.
FLOORS - SMOOTH, MOISTURE PROOF, NON-ABSORBENT, SKID-PROOF.

WALLS & CEILINGS - FREE OF CRACKS,
STORAGE AREAS - SEPARATE STORAGE AREAS SHOULD BE DESIGNED FOR FEED, BEDDING,
CAGES AND MATERIALS NOT IN USE.
EXPERIMENTAL AREA - SEPARATE AREA FROM THE PLACE WHERE ANIMALS ARE HOUSED.

ENVIRONMENT
TEMPERATURE AND HUMIDITY CONTROL :- AIR CONDITIONING IS EFFECTIVE IN REGULATING
ENVIRONMENTAL PARAMETERS.
TEMPERATURE RANGE- 18 TO 29°C (64.4 TO 84.2°F)
RELATIVE HUMIDITY RANGE- 30% TO 70%
VENTILATION :-THE ANIMAL FACILITY AND HUMAN OCCUPANCY AREAS SHOULD BE
VENTILATED SEPARATELY.

RECORD KEEPING
1) THE ANIMAL HOUSE SHOULD MAINTAIN FOLLOWING RECORDS :
2) ANIMAL HOUSE PLANS, WHICH INCLUDES TYPICAL FLOOR PLAN, ALL FIXTURES ETC.
3) ANIMAL HOUSE STAFF RECORD.
4) HEALTH RECORD OF STAFF & ANIMALS.
5) BREEDING, STOCK, PURCHASE AND SALES RECORDS.
6) RECORDS OF EXPERIMENTS CONDUCTED WITH THE NUMBER OF ANIMALS USED
7) DEATH RECORD.

STANDARD OPERATING PROCEDURE
SOP SHOULD CONTAIN FOLLOWING ITEMS :-
NAME OF AUTHOR
TITLE OF SOP
DATE OF PREPARATION
REFERENCE OF PREVIOUS SOP OBJECTIVES
DETAILED INFORMATION OF THE INSTRUMENTS USED IN RELATION WITH ANIMALS.

PERSONNEL AND TRAINING
THE STAFF MUST BE PROVIDED WITH ALL REQUIRED PROTECTIVE CLOTHING (MASKS, APRONS,
GLOVES AND GUMBOOTS AND OTHER FOOTWEAR) WHILE WORKING IN ANIMAL ROOMS.
REGULAR MEDICAL CHECK-UP IS ARRANGED FOR THE WORKERS.
PERSONS WORKING IN ANIMAL HOUSE DO NOT EAT, DRINK, SMOKE IN ANIMAL ROOM

TRANSPORT OF LAB ANIMALS
THE MODE OF TRANSPORT,
THE CONTAINERS,
THE ANIMAL DENSITY IN CAGES,
FOOD AND WATER DURING TRANSIT,
PROTECTION FROM INFECTION, INJURIES AND STRESS.

REFERENCES
S.K GUPTA, PHARMACOLOGICAL & TOXICOLOGICAL SCREENING METHODS
HTTPS://WWW.PCI.NIC.IN/PDF/15.PDF
KULKARNI S K, COMMON LABORATORY ANIMALS (1999) HANDBOOK OF EXPERIMENTAL
PHARMACOLOGY, VALLABH PRAKASHAN, DELHI 3:11-18.

THANK YOU.