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Annals. Food Science and Technology
2015
Available on-line at www.afst.valahia.ro Volume 16, Issue 1, 2015
11
INFLUENCE OF GAMMA RADIATION ON SOME TEXTURAL PROPERTIES OF
FRESH AND DRIED OYSTER MUSHROOMS (PLEUROTUS OSTREATUS)
Nii Korley Kortei1*
, George Tawia Odamtten1,2
, Mary Obodai 3
,Victoria Appiah1
, Lawrence Abbey3
,
Charlotte Oduro-Yeboah3
, Papa Toah Akonor3
1
Graduate School of Nuclear and Allied Sciences, Department of Nuclear Agriculture and Radiation Processing,
P.O. Box LG 80, Legon, Ghana.
2
University of Ghana, Department of Botany, P. O. Box LG 55, Legon, Ghana.
3
Food Research Institute, Council for Scientific and Industrial Research, P.O. Box M20, Accra, Ghana.
*E-mail: nii_korley_1@yahoo.com
Abstract
Food processing involves the input of thermal, mechanical, photonic or ionising radiation energy.Gamma irradiation is
usually perceived to energize and make foods radioactive which ultimately modify their chemical composition mainly
the texture. Texture profile analysis (TPA) was used to evaluate the texture parameters of hardness, fracturability,
cohesiveness, springiness, gumminess, chewiness, adhesiveness and resilience of the mushrooms. Fresh, dried and
rehydrated mushrooms (Pleurotus ostreatus) were exposed to gamma radiation doses of 0, 0.5, 1, 1.5 and 2 kGy at a
dose rate of 1.7 kGy hr-1
.The fresh products had an average moisture content of 80.3- 85.0% while dried mushrooms
had average moisture content of 11- 14%. Rehydrated mushrooms had moisture content range of 42.3- 49.7%.Dose of
1.5 kGy was most apparent on hardness, resilience, springiness and adhesiveness. Cohesiveness increased as dried
mushrooms were rehydrated. Hardness, fracturability, gumminess and chewiness decreased as radiation increased with
dried mushrooms. Although the results obtained showed that irradiation had significant (P<0.05) effects on fresh, dried
and rehydrated mushrooms, irradiation process is encouraged because of persistent high food losses from
infestation,contamination, and spoilage; food-borne diseases; and growing international trade in food products that
must meet strict import standards of quality and quarantine.
Key words- TPA, gamma, irradiation, Pleurotus ostreatus, texture, mushrooms
Submitted: 21.12.2014 Reviewed: 02.03.2015 Accepted: 24.04.2015
1. INTRODUCTION
Mushrooms are accepted or rejected on the
basis of their chemical and physical properties,
specially the texture, a physical quality very
important in the case of mushrooms (Matser et
al., 2000). Mushrooms are soft textured and
prone to deterioration shortly after harvest if
not stored properly (Walde, 2006). In view of
their short shelf life under normal ambient
conditions of temperature and humidity, it is
imperative to preserve it by processing to
extend shelf life for off season use
(Kotwaliwale et al, 2010). Dehydration,
canning, freezing, among others, have been
found to be suitable for mushroom preservation
(Bernas, 2006; Pal, 1997). Nonetheless, among
the various methods employed for preservation,
drying is one of the common methods used for
mushrooms owing to its easiness and
economical nature (Kumar et al., 2013;
Argyropoulos et al., 2011; Kotwaliwale et al.,
2007; Walde et al., 2006; Kar et al., 2004; Rai
et al., 2003).The dehydrated product offers,
apart from increased shelf-life, the advantages
of decreased mass and volume which have the
potential for savings in the cost of packaging,
handling, storage and transport of the product
(Karimi, 2010; Amuthan et al., 1999).
To complement the drying method, irradiation
technology could be employed for controlling
insect infestation, reduce the numbers of
pathogenic or spoilage microorganisms, and
delay or eliminate natural biological processes
such as ripening, germination, or sprouting in
fresh food. Like all preservation methods,
irradiation should supplement rather than
replace good food hygiene, handling, and
preparation practices (Food Safety Authority of
Ireland [FSAI], 2005).The FDA emphasizes
 2015 Valahia University Press
Further reproduction without permission is prohibited
Annals. Food Science and Technology
2015
Available on-line at www.afst.valahia.ro Volume 16, Issue 1, 2015
12
that no preservation method is a substitute for
safe food handling procedures (Arvanitoyannis,
2010).
Texture is composed of several textural
properties which involve mechanical (hardness,
chewiness, and viscosity), geometrical (particle
size and shape) and chemical (moisture and fat
content) characteristics (Bourne, 1980).The
texture of food material is mainly addressed to
describe the structure of the food and the
manner in which that structure reacts to applied
force (Szczeoeniak, 1968).The texture
parameter, together with appearance and
flavour, are the organoleptic quality attributes
which determine the acceptability of a food by
the consumer (Guine and Barroca, 2011).One
of the main changes associated with
mushrooms deterioration are changes in their
texture (Lopez-Briones et al, 1992; Villaescusa
and Gil, 2003; Ares et al, 2006; Parentelli et al,
2007).Instrumental measurements of texture
have become essential for the assessment of
quality in the food industry (Harker et al, 2006)
and, among the methods used to determine
texture, instrumental texture profile analysis
(TPA) is the most frequently used to calculate
the textural properties, and intend to imitate the
repeated biting or chewing of a food (Guine
and Barroca, 2011).Also, instrumental
measurements of texture are preferred over
sensory evaluations since instruments may
reduce variation among measurements due to
human factors and are more precise (Casas et
al., 2006).
The aim of the present study was to assess the
effect of physical stress (gamma irradiation) on
the texture of fresh, solar dried and rehydrated
mushrooms (Pleurotus ostreatus) by measuring
their textural attributes (hardness,
fracturability, springiness, cohesiveness,
chewiness, gumminess, adhesiveness and
resilience) using texture profile analysis.
2. MATERIALS AND METHODS
Sampling
Fresh oyster mushrooms of average sizes were
obtained from the Mycology Unit of the Food
Research Institute, Ghana. Mushrooms of stipe
diameter of about 0.7cm were selected and
used in the analysis. Averages of five
mushroom fruit bodies were used in the
analysis and the mean values determined. They
were washed thoroughly under running tap
water to remove adhering extraneous matter
and then used for the experimental studies.
Processing
Drying of mushroom samples
Drying was carried out by using a solar dryer at
a temperature of 50- 60o
C to reduce moisture
content to about 12% for an average period of
12 days.
Irradiation of mushroom materials
Forty (40) grams of dried oyster mushrooms
(Pleurotus ostreatus) were packed into the
various containers (polythene and
polypropylene) and irradiated at doses of 0,
0.5, 1, 1.5 and 2 kGy at a dose rate of 1.7 kGy
per hour in air from a Cobalt 60source (SLL
515, Hungary) doses were confirmed using the
ethanol-chlorobenzene (ECB) dosimetry
system at the Radiation Technology Centre of
the Ghana Atomic Energy Commission, Accra,
Ghana.
Rehydration of mushrooms
Rehydration of mushrooms was determined by
a modified method of Giri and Prasad, (2013).
Exactly 5g of dried samples were immersed in
distilled water at 90- 100o
C temperature. The
water was drained and the samples weighed at
every 2 min intervals until constant weight was
attained. Moisture content was about 42.3-
49.7%. Triplicate samples were used.
Determination of moisture content
The moisture content was determined by the
gravimetric method of (AOAC, 1996).
Instrumental Texture measurement
Texture Analyser (make: Stable Micro
Systems, UK—Model: TA-XT2i) was used for
texture analysis of all the samples. Texture
analysis was carried out under following
Annals. Food Science and Technology
2015
Available on-line at www.afst.valahia.ro Volume 16, Issue 1, 2015
13
instrument parameters: pre-test speed—10
mm/s; testspeed—2 mm/s; post-test speed—10
mm/sec; time lag between two compressions—
2 sec; strain—30% of sample height; trigger
force—0.05 N; data acquisition rate—200 pps;
10 mm hemispherical plastic probe; load cell—
25 kg with 1 g least count. Hardness,
springiness, cohesiveness, and chewiness of the
samples were calculated using the expressions
as shown in Fig. 1.
The textural properties: hardness, adhesiveness,
springiness, cohesiveness, and chewiness were
calculated after (1) to (5) (see Fig. 1):
Hardness (kgf) = F1 ………………………(1)
Adhesiveness (kgf.s) = A3 ……………….(2)
Springiness = (t2/t1)…… …………… (3)
Cohesiveness (dimensionless) = A2/A1…. (4)
Chewiness (kg.f) = (F1) (t2/t1) (A2/A1)… (5)
where F1 is the maximum force, i.e., the force
in the first peak, A1 and A2 are the areas of the
first and second peaks, respectively, and T1
and T2 are the time intervals for the first and
second peaks, respectively. The area of the
negative peak, that should be visible between
vertical lines 3 and 4 (the vertical lines are
auxiliary to compute the textural parameters),
represents adhesiveness, and would be visible
only when the food has measurable
adhesiveness, which was sparingly recorded.
3. RESULTS AND DISCUSSIONS
Table 1. Shows the moisture content of fresh
mushrooms which ranged 80.8-83.7%;dried
mushrooms recorded 11.5-13.1% while
rehydrated mushrooms also recorded 42.3-
49.7%. According to Ares et al, (2007),
mushrooms are only protected by a thin and
porous epidermal structure, lacking the
specialized epidermal structure of higher plant
tissues. This epidermal layer does not avoid a
quick superficial dehydration causes important
quality losses (Singer, 1986). Post harvest
senescence in a variety of horticultural
commodities is accompanied by changes in cell
membrane characteristics, which leads to loss
of barrier function and loss of turgor (Mazliak,
1987)..
Fig.1. Texture Profile Analysis (TPA) (Source: Anonymous 2005)
Annals. Food Science and Technology
2015
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14
Softening of mushrooms and firmness loss
during postharvest storage has been ascribed to
membrane changes which are directly linked to
moisture content of cells(Beelman, 1987;
Waldron, 1997; Marsilio, 2000)
Table 1. Mean moisture content (%) of fresh, dried and rehydrated irradiated oyster mushrooms
Dose (kGy) Moisture content (%)Fresh Dried Rehydrated
0 80.8 12.443.2
0.5 81.3 11.545.6
1 83.7 12.548.6
1.5 81.5 13.1 42.3
2 80.9 12.8 49.7
S.D 1.3 0.4 1.1
Mean 81.6 12.5 45.9
Fig.2: Texture profile analysis curve of fresh oyster mushrooms irradiated at 2 kGy
Fig.3. Texture profile analysis curve of dried oyster mushrooms irradiated at 2 kGy
0 10 2 0 3 0 4 0 5 0 6 0 7 0
12
10
8
6
4
2
0
- 2
Fo rc e (N)
Time (s ec .)
1f
2f
3f
4f
1 2 3 4 5 67 8
0 10 2 0 3 0 4 0 5 0 6 0 7 0
18 0
16 0
14 0
12 0
10 0
8 0
6 0
4 0
2 0
0
- 2 0
Fo rc e (N)
Time (s ec .)
1f
2f3f
4f
1 2 3 4 5 67 8
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2015
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Influence of irradiation on the texture of fresh,
dried and rehydrated mushrooms
Texture results from complex interactions
between food components, and is affected by
cellular organelles and biochemical
constituents, water content as well as and cell
wall composition. Hence, external factors
affecting these qualities can modify texture
(Guiné and Barroca, 2011). The changes in
texture that occur during the processing of
mushroom materials or certain physiological
events have been related with tissue and cell
micro-structural changes (Marsilio et al,
2000).The effect of processing treatments such
as irradiation, dehydrating and rehydrating had
significant (P< 0.05) on the textural parameters
of the Pleurotus ostreatus and the results
evaluated. The results are presented in Figures
2-11.
Hardness
The hardness of the samples taken from
irradiated fresh, dried and rehydrated
mushrooms are presented in Fig. 2. This
property corresponds to the maximum force
recorded during the first cycle of compression,
and represents the force required between the
molars for chewing a food, being in most cases
related to the tensile strength of the sample.
Generally, higher values were recorded for
dried mushrooms (Fig.3) and there were no
significant (P> 0.05) differences between doses
0, 0.5, 1 and 1.5 kGy which ranged between
63- 74 kgf. Irradiation dose of 2 kGy however
showed an apparent decrease in hardness of the
dried samples to a low value of 29 kgf. Fresh
mushrooms recorded low values of the range
0.2- 0.4 kgf. There were significant (P< 0.05)
differences among the treatment doses.
Rehydrated mushrooms also recorded similarly
low values of 0.1- 0.2 kgf which were not
significantly different (P> 0.05). Hardness in
dried mushrooms decreased from 1.5 kGy and
above doses (Fig.4). This might be due to the
hydrolytic effect of gamma radiations on the
cell membranes (Mamiet al, 2013; Gbedemah
et al, 1998). Giri and Prasad, (2013) recorded
higher values in their work. While Kotwaliwale
et al, 2007 recorded lower values.
Fig. 4: Influence of gamma radiation on Hardness of
oyster mushrooms
Fracturability
Not all products fracture; but when they do
fracture the fracturability point occurs where
the plot has its first significant peak (where the
force falls off) during the probe's first
compression of the product. Dried mushrooms
irradiated with 0, 0.5 and 1 kGy doses of
radiation required a force of 14.8- 15.9 N and
were not significantly different (P>0.05)
(Fig.5). However, the force decreased to 12.9-
13.2 N as the doses were increased to 1.5 and 2
kGy which recorded no significant difference
(P> 0.05). Fresh and rehydrated mushrooms
recorded minimal forces of 0 - 1.7 N as
radiation doses increased (Fig.5). Statistical
analysis showed no significant difference
(P>0.05). This is suggestive of increased
gamma radiation doses having an apparent
effect on protein and polysaccharide
degradation, hyphae shrinkage, central vacuole
disruption and expansion of intercellular space
(Zivanovic et al, 2000).
Fig. 5: Influence of gamma radiation on
Fracturability of oyster mushrooms
0
20
40
60
80
100
0 0.5 1 1.5 2
Hardness(kgf)
Dose ( kGy )
Fresh
Dried
Rehydrated
-5
0
5
10
15
20
0 0.5 1 1.5 2
Fracturability(N)
Dose ( kGy )
Fresh
Dried
Rehydrated
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2015
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Cohesiveness
Cohesiveness represents the ratio between the
work done in the second compression and the
work done in the first compression, and reflects
the ability of the product to stay as one. The
cohesiveness of dried mushrooms ranged
between 0.6- 0.78 (Fig.6). The various doses
showed significant differences (P< 0.05). Fresh
mushroom cohesiveness also ranged0.65- 0.8
which were comparable to the rehydrated
oyster mushrooms. Generally, cohesiveness of
rehydrated mushrooms was increased and so
ranged between 0.75- 0.86 and showed
significant differences with respect to the
various doses (Fig.6). Our results are in
agreement with some researchers (Kotwaliwale
et al, 2001; Zivanovic et al, 2000; Parentelli et
al, 2007) who recorded an increase in
cohesiveness after rehydration. This trend has
been attributed to an increase in chitin content
and formation of covalent bonds between chitin
and β-glucan, increasing rigidity of the hyphal
wall (Zivanovic et al, 2000).
Fig.6: Influence of gamma radiation on cohesiveness
of oyster mushrooms
Chewiness
Chewiness represents the energy required to
disintegrate a solid material in order to swallow
it and data is presented for the fresh, dried and
rehydrated mushrooms at study in Fig.7. The
dried mushrooms recorded highest values of
chewiness of ranged from 5-16N and showed
significant differences (P<0.05) (Fig.7).Fresh
and rehydrated mushrooms also ranged 0.1- 2.0
N and 0.2- 1.9 N respectively and also showed
significant differences (P<0.05). Values
recorded for fresh mushrooms were consistent
with data from the paper of Santos et al,
(2012).
Fig.7: Influence of gamma radiation on Chewiness of
oyster mushrooms
Springiness
Springiness is the ratio between the times in the
two deformations, and represents the ability to
regain shape when the deforming stress is
removed or reduced, i.e., expresses the percent
of recovery of the sample. Generally, dried
mushrooms recorded comparatively lower
values of the range 0.23- 0.31mm(Fig.8) while
fresh and rehydrated mushrooms ranged
between 0.12- 0.51 mm and 0.14- 0.50 mm
respectively (Fig.8). Statistically, there was no
difference (P>0.05) between fresh and
rehydrated but differed (P<0.05) from the dried
samples. The ability of the fresh and rehydrated
oyster mushrooms to regain their shapes after
the stress is reduced is suggestive of the
presence of moisture in the cells of the
mushrooms which causes turgidity to aid in
strengthening the cell walls. The results for
fresh and rehydrated oyster mushroom were
similar to the data of Riebroy et al, (2010).
Conversely, dried and irradiated mushrooms
have depleted moisture to a stage that capillary
voids were created hence reduced springiness.
This trend is in agrees with data from studies
by Kotwaliwale et al, (2007).
0
0.2
0.4
0.6
0.8
1
0 0.5 1 1.5 2
Cohesiveness
Dose ( kGy )
Fresh
Dried
Rehydrated
-5
0
5
10
15
20
0 0.5 1 1.5 2
Chewiness(N) Dose (kGy)
Fresh
Dried
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2015
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Fig.8: Influence of gamma radiation on Springiness
of oyster mushroom
Gumminess
Gumminess is mutually exclusive with
Chewiness since a product would not be both a
semi-solid and a solid at the same time. It is a
product of Hardness and Cohesiveness (which
is Area 2/Area1) (Fig.1).The gumminess of
dried mushrooms ranged between 30- 38. Fresh
and rehydrated mushrooms also ranged
between 1.0- 5.0 and 1- 3 respectively. There
was an apparent difference in Gumminess of
dried mushrooms generally decreased with
increasing radiation doses and recorded
significant changes (P<0.05). Gamma
radiations might have caused a weakening of
the intermolecular bonds and so slightly
affected (IAEA, 1999).
Adhesiveness
Adhesiveness represents the work necessary to
overcome the forces of attraction between the
sample and the probe surface, and is given by
the value of the area corresponding to the
negative force (A3 in Fig. 1). Fig.9 shows the
absolute values for adhesiveness in the fresh,
dried and rehydrated mushroom samples when
applicable, since in most of the cases the
rehydrated samples had highest values of range
-0.2 and -0.8 kgf.s. Radiation doses showed
significant differences (P<0.05). The results
show that, even in the case of the dried and
irradiated, the adhesiveness was very low (-
0.002 and -0.009), almost negligible.
Statistically, there was no significant difference
(P>0.05) between all the applied doses. The
values were consistent with works of Guine
and Barroca (2011) and Riebroy et al, (2010).
Fig.9: Influence of gamma radiation on the
adhesiveness of mushrooms
Fig.10: Influence of gamma radiation on Gumminess
of oyster mushroom
Resilience
Resilience is how well a product "fights to
regain its original position". The calculation is
the area during the withdrawal of the first
compression, divided by the area of the first
compression. (Area 5/Area4 on Fig.1). Fresh
mushrooms demonstrated highest (0.84- 1.71)
resilience for all radiation doses. Doses 0, 0.5,
1 and 2 kGy showed no significant difference
(P>0.05) (Fig.10). However, dose of 1.5 kGy
showed significant difference between other
doses (Fig.10).The ability of fresh and
irradiated mushrooms to exhibit high resilience
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0 0.5 1 1.5 2
Springiness(mm)
Dose ( kGy )
Fresh
Dried
Rehydrated
-1
-0.8
-0.6
-0.4
-0.2
0
0 0.5 1 1.5 2
Adhesiveness(kgf.s)
Dose (kGy)
fresh
Dried
Rehydrated
0
10
20
30
40
50
0 0.5 1 1.5 2
Gumminess
Dose ( kGy)
Fresh
Dried
Rehydrated
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could be ascribed to its original and unaltered
cell membrane as it has not undergone
senescence (Beelman et al, 1987).Recorded
values were consistent with Riebroy et al,
(2010). Dried and rehydrated mushrooms
recorded resilience of ranges 0.42- 0.51 and
0.30- 0.41 respectively. Dehydration has a very
pronounced effect on the structure of foods,
due to the loss of a considerable amount of
water, increasing density by shrinkage in the
case of convective drying. Apparently,
rehydrated mushrooms could not regain its
original shape after subjection to stress
probably because water molecules could not
fill all the pore spaces of the cell membrane
and so turgidity was minimal.
Fig.11: Influence of gamma radiation on the
resilience of mushrooms
4. CONCLUSION
The Textural Profile Analysis results obtained
for the parameters investigated for Pleurotus
ostreatus revealed an apparent effect of gamma
radiation on the fresh, dried and rehydrated
states of the mushroom. Gamma radiation
could however be used to prolong shelf life of
mushrooms during storage.
5. ACKNOWLEDGEMENT
Authors would like to express their gratitude to
all technicians at the Department of Food
Processing and Engineering, Food Research
Institute, CSIR, Accra for their technical
assistance.
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Nii korley kortei (texture)

  • 1. Annals. Food Science and Technology 2015 Available on-line at www.afst.valahia.ro Volume 16, Issue 1, 2015 11 INFLUENCE OF GAMMA RADIATION ON SOME TEXTURAL PROPERTIES OF FRESH AND DRIED OYSTER MUSHROOMS (PLEUROTUS OSTREATUS) Nii Korley Kortei1* , George Tawia Odamtten1,2 , Mary Obodai 3 ,Victoria Appiah1 , Lawrence Abbey3 , Charlotte Oduro-Yeboah3 , Papa Toah Akonor3 1 Graduate School of Nuclear and Allied Sciences, Department of Nuclear Agriculture and Radiation Processing, P.O. Box LG 80, Legon, Ghana. 2 University of Ghana, Department of Botany, P. O. Box LG 55, Legon, Ghana. 3 Food Research Institute, Council for Scientific and Industrial Research, P.O. Box M20, Accra, Ghana. *E-mail: nii_korley_1@yahoo.com Abstract Food processing involves the input of thermal, mechanical, photonic or ionising radiation energy.Gamma irradiation is usually perceived to energize and make foods radioactive which ultimately modify their chemical composition mainly the texture. Texture profile analysis (TPA) was used to evaluate the texture parameters of hardness, fracturability, cohesiveness, springiness, gumminess, chewiness, adhesiveness and resilience of the mushrooms. Fresh, dried and rehydrated mushrooms (Pleurotus ostreatus) were exposed to gamma radiation doses of 0, 0.5, 1, 1.5 and 2 kGy at a dose rate of 1.7 kGy hr-1 .The fresh products had an average moisture content of 80.3- 85.0% while dried mushrooms had average moisture content of 11- 14%. Rehydrated mushrooms had moisture content range of 42.3- 49.7%.Dose of 1.5 kGy was most apparent on hardness, resilience, springiness and adhesiveness. Cohesiveness increased as dried mushrooms were rehydrated. Hardness, fracturability, gumminess and chewiness decreased as radiation increased with dried mushrooms. Although the results obtained showed that irradiation had significant (P<0.05) effects on fresh, dried and rehydrated mushrooms, irradiation process is encouraged because of persistent high food losses from infestation,contamination, and spoilage; food-borne diseases; and growing international trade in food products that must meet strict import standards of quality and quarantine. Key words- TPA, gamma, irradiation, Pleurotus ostreatus, texture, mushrooms Submitted: 21.12.2014 Reviewed: 02.03.2015 Accepted: 24.04.2015 1. INTRODUCTION Mushrooms are accepted or rejected on the basis of their chemical and physical properties, specially the texture, a physical quality very important in the case of mushrooms (Matser et al., 2000). Mushrooms are soft textured and prone to deterioration shortly after harvest if not stored properly (Walde, 2006). In view of their short shelf life under normal ambient conditions of temperature and humidity, it is imperative to preserve it by processing to extend shelf life for off season use (Kotwaliwale et al, 2010). Dehydration, canning, freezing, among others, have been found to be suitable for mushroom preservation (Bernas, 2006; Pal, 1997). Nonetheless, among the various methods employed for preservation, drying is one of the common methods used for mushrooms owing to its easiness and economical nature (Kumar et al., 2013; Argyropoulos et al., 2011; Kotwaliwale et al., 2007; Walde et al., 2006; Kar et al., 2004; Rai et al., 2003).The dehydrated product offers, apart from increased shelf-life, the advantages of decreased mass and volume which have the potential for savings in the cost of packaging, handling, storage and transport of the product (Karimi, 2010; Amuthan et al., 1999). To complement the drying method, irradiation technology could be employed for controlling insect infestation, reduce the numbers of pathogenic or spoilage microorganisms, and delay or eliminate natural biological processes such as ripening, germination, or sprouting in fresh food. Like all preservation methods, irradiation should supplement rather than replace good food hygiene, handling, and preparation practices (Food Safety Authority of Ireland [FSAI], 2005).The FDA emphasizes  2015 Valahia University Press Further reproduction without permission is prohibited
  • 2. Annals. Food Science and Technology 2015 Available on-line at www.afst.valahia.ro Volume 16, Issue 1, 2015 12 that no preservation method is a substitute for safe food handling procedures (Arvanitoyannis, 2010). Texture is composed of several textural properties which involve mechanical (hardness, chewiness, and viscosity), geometrical (particle size and shape) and chemical (moisture and fat content) characteristics (Bourne, 1980).The texture of food material is mainly addressed to describe the structure of the food and the manner in which that structure reacts to applied force (Szczeoeniak, 1968).The texture parameter, together with appearance and flavour, are the organoleptic quality attributes which determine the acceptability of a food by the consumer (Guine and Barroca, 2011).One of the main changes associated with mushrooms deterioration are changes in their texture (Lopez-Briones et al, 1992; Villaescusa and Gil, 2003; Ares et al, 2006; Parentelli et al, 2007).Instrumental measurements of texture have become essential for the assessment of quality in the food industry (Harker et al, 2006) and, among the methods used to determine texture, instrumental texture profile analysis (TPA) is the most frequently used to calculate the textural properties, and intend to imitate the repeated biting or chewing of a food (Guine and Barroca, 2011).Also, instrumental measurements of texture are preferred over sensory evaluations since instruments may reduce variation among measurements due to human factors and are more precise (Casas et al., 2006). The aim of the present study was to assess the effect of physical stress (gamma irradiation) on the texture of fresh, solar dried and rehydrated mushrooms (Pleurotus ostreatus) by measuring their textural attributes (hardness, fracturability, springiness, cohesiveness, chewiness, gumminess, adhesiveness and resilience) using texture profile analysis. 2. MATERIALS AND METHODS Sampling Fresh oyster mushrooms of average sizes were obtained from the Mycology Unit of the Food Research Institute, Ghana. Mushrooms of stipe diameter of about 0.7cm were selected and used in the analysis. Averages of five mushroom fruit bodies were used in the analysis and the mean values determined. They were washed thoroughly under running tap water to remove adhering extraneous matter and then used for the experimental studies. Processing Drying of mushroom samples Drying was carried out by using a solar dryer at a temperature of 50- 60o C to reduce moisture content to about 12% for an average period of 12 days. Irradiation of mushroom materials Forty (40) grams of dried oyster mushrooms (Pleurotus ostreatus) were packed into the various containers (polythene and polypropylene) and irradiated at doses of 0, 0.5, 1, 1.5 and 2 kGy at a dose rate of 1.7 kGy per hour in air from a Cobalt 60source (SLL 515, Hungary) doses were confirmed using the ethanol-chlorobenzene (ECB) dosimetry system at the Radiation Technology Centre of the Ghana Atomic Energy Commission, Accra, Ghana. Rehydration of mushrooms Rehydration of mushrooms was determined by a modified method of Giri and Prasad, (2013). Exactly 5g of dried samples were immersed in distilled water at 90- 100o C temperature. The water was drained and the samples weighed at every 2 min intervals until constant weight was attained. Moisture content was about 42.3- 49.7%. Triplicate samples were used. Determination of moisture content The moisture content was determined by the gravimetric method of (AOAC, 1996). Instrumental Texture measurement Texture Analyser (make: Stable Micro Systems, UK—Model: TA-XT2i) was used for texture analysis of all the samples. Texture analysis was carried out under following
  • 3. Annals. Food Science and Technology 2015 Available on-line at www.afst.valahia.ro Volume 16, Issue 1, 2015 13 instrument parameters: pre-test speed—10 mm/s; testspeed—2 mm/s; post-test speed—10 mm/sec; time lag between two compressions— 2 sec; strain—30% of sample height; trigger force—0.05 N; data acquisition rate—200 pps; 10 mm hemispherical plastic probe; load cell— 25 kg with 1 g least count. Hardness, springiness, cohesiveness, and chewiness of the samples were calculated using the expressions as shown in Fig. 1. The textural properties: hardness, adhesiveness, springiness, cohesiveness, and chewiness were calculated after (1) to (5) (see Fig. 1): Hardness (kgf) = F1 ………………………(1) Adhesiveness (kgf.s) = A3 ……………….(2) Springiness = (t2/t1)…… …………… (3) Cohesiveness (dimensionless) = A2/A1…. (4) Chewiness (kg.f) = (F1) (t2/t1) (A2/A1)… (5) where F1 is the maximum force, i.e., the force in the first peak, A1 and A2 are the areas of the first and second peaks, respectively, and T1 and T2 are the time intervals for the first and second peaks, respectively. The area of the negative peak, that should be visible between vertical lines 3 and 4 (the vertical lines are auxiliary to compute the textural parameters), represents adhesiveness, and would be visible only when the food has measurable adhesiveness, which was sparingly recorded. 3. RESULTS AND DISCUSSIONS Table 1. Shows the moisture content of fresh mushrooms which ranged 80.8-83.7%;dried mushrooms recorded 11.5-13.1% while rehydrated mushrooms also recorded 42.3- 49.7%. According to Ares et al, (2007), mushrooms are only protected by a thin and porous epidermal structure, lacking the specialized epidermal structure of higher plant tissues. This epidermal layer does not avoid a quick superficial dehydration causes important quality losses (Singer, 1986). Post harvest senescence in a variety of horticultural commodities is accompanied by changes in cell membrane characteristics, which leads to loss of barrier function and loss of turgor (Mazliak, 1987).. Fig.1. Texture Profile Analysis (TPA) (Source: Anonymous 2005)
  • 4. Annals. Food Science and Technology 2015 Available on-line at www.afst.valahia.ro Volume 16, Issue 1, 2015 14 Softening of mushrooms and firmness loss during postharvest storage has been ascribed to membrane changes which are directly linked to moisture content of cells(Beelman, 1987; Waldron, 1997; Marsilio, 2000) Table 1. Mean moisture content (%) of fresh, dried and rehydrated irradiated oyster mushrooms Dose (kGy) Moisture content (%)Fresh Dried Rehydrated 0 80.8 12.443.2 0.5 81.3 11.545.6 1 83.7 12.548.6 1.5 81.5 13.1 42.3 2 80.9 12.8 49.7 S.D 1.3 0.4 1.1 Mean 81.6 12.5 45.9 Fig.2: Texture profile analysis curve of fresh oyster mushrooms irradiated at 2 kGy Fig.3. Texture profile analysis curve of dried oyster mushrooms irradiated at 2 kGy 0 10 2 0 3 0 4 0 5 0 6 0 7 0 12 10 8 6 4 2 0 - 2 Fo rc e (N) Time (s ec .) 1f 2f 3f 4f 1 2 3 4 5 67 8 0 10 2 0 3 0 4 0 5 0 6 0 7 0 18 0 16 0 14 0 12 0 10 0 8 0 6 0 4 0 2 0 0 - 2 0 Fo rc e (N) Time (s ec .) 1f 2f3f 4f 1 2 3 4 5 67 8
  • 5. Annals. Food Science and Technology 2015 Available on-line at www.afst.valahia.ro Volume 16, Issue 1, 2015 15 Influence of irradiation on the texture of fresh, dried and rehydrated mushrooms Texture results from complex interactions between food components, and is affected by cellular organelles and biochemical constituents, water content as well as and cell wall composition. Hence, external factors affecting these qualities can modify texture (Guiné and Barroca, 2011). The changes in texture that occur during the processing of mushroom materials or certain physiological events have been related with tissue and cell micro-structural changes (Marsilio et al, 2000).The effect of processing treatments such as irradiation, dehydrating and rehydrating had significant (P< 0.05) on the textural parameters of the Pleurotus ostreatus and the results evaluated. The results are presented in Figures 2-11. Hardness The hardness of the samples taken from irradiated fresh, dried and rehydrated mushrooms are presented in Fig. 2. This property corresponds to the maximum force recorded during the first cycle of compression, and represents the force required between the molars for chewing a food, being in most cases related to the tensile strength of the sample. Generally, higher values were recorded for dried mushrooms (Fig.3) and there were no significant (P> 0.05) differences between doses 0, 0.5, 1 and 1.5 kGy which ranged between 63- 74 kgf. Irradiation dose of 2 kGy however showed an apparent decrease in hardness of the dried samples to a low value of 29 kgf. Fresh mushrooms recorded low values of the range 0.2- 0.4 kgf. There were significant (P< 0.05) differences among the treatment doses. Rehydrated mushrooms also recorded similarly low values of 0.1- 0.2 kgf which were not significantly different (P> 0.05). Hardness in dried mushrooms decreased from 1.5 kGy and above doses (Fig.4). This might be due to the hydrolytic effect of gamma radiations on the cell membranes (Mamiet al, 2013; Gbedemah et al, 1998). Giri and Prasad, (2013) recorded higher values in their work. While Kotwaliwale et al, 2007 recorded lower values. Fig. 4: Influence of gamma radiation on Hardness of oyster mushrooms Fracturability Not all products fracture; but when they do fracture the fracturability point occurs where the plot has its first significant peak (where the force falls off) during the probe's first compression of the product. Dried mushrooms irradiated with 0, 0.5 and 1 kGy doses of radiation required a force of 14.8- 15.9 N and were not significantly different (P>0.05) (Fig.5). However, the force decreased to 12.9- 13.2 N as the doses were increased to 1.5 and 2 kGy which recorded no significant difference (P> 0.05). Fresh and rehydrated mushrooms recorded minimal forces of 0 - 1.7 N as radiation doses increased (Fig.5). Statistical analysis showed no significant difference (P>0.05). This is suggestive of increased gamma radiation doses having an apparent effect on protein and polysaccharide degradation, hyphae shrinkage, central vacuole disruption and expansion of intercellular space (Zivanovic et al, 2000). Fig. 5: Influence of gamma radiation on Fracturability of oyster mushrooms 0 20 40 60 80 100 0 0.5 1 1.5 2 Hardness(kgf) Dose ( kGy ) Fresh Dried Rehydrated -5 0 5 10 15 20 0 0.5 1 1.5 2 Fracturability(N) Dose ( kGy ) Fresh Dried Rehydrated
  • 6. Annals. Food Science and Technology 2015 Available on-line at www.afst.valahia.ro Volume 16, Issue 1, 2015 16 Cohesiveness Cohesiveness represents the ratio between the work done in the second compression and the work done in the first compression, and reflects the ability of the product to stay as one. The cohesiveness of dried mushrooms ranged between 0.6- 0.78 (Fig.6). The various doses showed significant differences (P< 0.05). Fresh mushroom cohesiveness also ranged0.65- 0.8 which were comparable to the rehydrated oyster mushrooms. Generally, cohesiveness of rehydrated mushrooms was increased and so ranged between 0.75- 0.86 and showed significant differences with respect to the various doses (Fig.6). Our results are in agreement with some researchers (Kotwaliwale et al, 2001; Zivanovic et al, 2000; Parentelli et al, 2007) who recorded an increase in cohesiveness after rehydration. This trend has been attributed to an increase in chitin content and formation of covalent bonds between chitin and β-glucan, increasing rigidity of the hyphal wall (Zivanovic et al, 2000). Fig.6: Influence of gamma radiation on cohesiveness of oyster mushrooms Chewiness Chewiness represents the energy required to disintegrate a solid material in order to swallow it and data is presented for the fresh, dried and rehydrated mushrooms at study in Fig.7. The dried mushrooms recorded highest values of chewiness of ranged from 5-16N and showed significant differences (P<0.05) (Fig.7).Fresh and rehydrated mushrooms also ranged 0.1- 2.0 N and 0.2- 1.9 N respectively and also showed significant differences (P<0.05). Values recorded for fresh mushrooms were consistent with data from the paper of Santos et al, (2012). Fig.7: Influence of gamma radiation on Chewiness of oyster mushrooms Springiness Springiness is the ratio between the times in the two deformations, and represents the ability to regain shape when the deforming stress is removed or reduced, i.e., expresses the percent of recovery of the sample. Generally, dried mushrooms recorded comparatively lower values of the range 0.23- 0.31mm(Fig.8) while fresh and rehydrated mushrooms ranged between 0.12- 0.51 mm and 0.14- 0.50 mm respectively (Fig.8). Statistically, there was no difference (P>0.05) between fresh and rehydrated but differed (P<0.05) from the dried samples. The ability of the fresh and rehydrated oyster mushrooms to regain their shapes after the stress is reduced is suggestive of the presence of moisture in the cells of the mushrooms which causes turgidity to aid in strengthening the cell walls. The results for fresh and rehydrated oyster mushroom were similar to the data of Riebroy et al, (2010). Conversely, dried and irradiated mushrooms have depleted moisture to a stage that capillary voids were created hence reduced springiness. This trend is in agrees with data from studies by Kotwaliwale et al, (2007). 0 0.2 0.4 0.6 0.8 1 0 0.5 1 1.5 2 Cohesiveness Dose ( kGy ) Fresh Dried Rehydrated -5 0 5 10 15 20 0 0.5 1 1.5 2 Chewiness(N) Dose (kGy) Fresh Dried
  • 7. Annals. Food Science and Technology 2015 Available on-line at www.afst.valahia.ro Volume 16, Issue 1, 2015 17 Fig.8: Influence of gamma radiation on Springiness of oyster mushroom Gumminess Gumminess is mutually exclusive with Chewiness since a product would not be both a semi-solid and a solid at the same time. It is a product of Hardness and Cohesiveness (which is Area 2/Area1) (Fig.1).The gumminess of dried mushrooms ranged between 30- 38. Fresh and rehydrated mushrooms also ranged between 1.0- 5.0 and 1- 3 respectively. There was an apparent difference in Gumminess of dried mushrooms generally decreased with increasing radiation doses and recorded significant changes (P<0.05). Gamma radiations might have caused a weakening of the intermolecular bonds and so slightly affected (IAEA, 1999). Adhesiveness Adhesiveness represents the work necessary to overcome the forces of attraction between the sample and the probe surface, and is given by the value of the area corresponding to the negative force (A3 in Fig. 1). Fig.9 shows the absolute values for adhesiveness in the fresh, dried and rehydrated mushroom samples when applicable, since in most of the cases the rehydrated samples had highest values of range -0.2 and -0.8 kgf.s. Radiation doses showed significant differences (P<0.05). The results show that, even in the case of the dried and irradiated, the adhesiveness was very low (- 0.002 and -0.009), almost negligible. Statistically, there was no significant difference (P>0.05) between all the applied doses. The values were consistent with works of Guine and Barroca (2011) and Riebroy et al, (2010). Fig.9: Influence of gamma radiation on the adhesiveness of mushrooms Fig.10: Influence of gamma radiation on Gumminess of oyster mushroom Resilience Resilience is how well a product "fights to regain its original position". The calculation is the area during the withdrawal of the first compression, divided by the area of the first compression. (Area 5/Area4 on Fig.1). Fresh mushrooms demonstrated highest (0.84- 1.71) resilience for all radiation doses. Doses 0, 0.5, 1 and 2 kGy showed no significant difference (P>0.05) (Fig.10). However, dose of 1.5 kGy showed significant difference between other doses (Fig.10).The ability of fresh and irradiated mushrooms to exhibit high resilience 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0 0.5 1 1.5 2 Springiness(mm) Dose ( kGy ) Fresh Dried Rehydrated -1 -0.8 -0.6 -0.4 -0.2 0 0 0.5 1 1.5 2 Adhesiveness(kgf.s) Dose (kGy) fresh Dried Rehydrated 0 10 20 30 40 50 0 0.5 1 1.5 2 Gumminess Dose ( kGy) Fresh Dried Rehydrated
  • 8. Annals. Food Science and Technology 2015 Available on-line at www.afst.valahia.ro Volume 16, Issue 1, 2015 18 could be ascribed to its original and unaltered cell membrane as it has not undergone senescence (Beelman et al, 1987).Recorded values were consistent with Riebroy et al, (2010). Dried and rehydrated mushrooms recorded resilience of ranges 0.42- 0.51 and 0.30- 0.41 respectively. Dehydration has a very pronounced effect on the structure of foods, due to the loss of a considerable amount of water, increasing density by shrinkage in the case of convective drying. Apparently, rehydrated mushrooms could not regain its original shape after subjection to stress probably because water molecules could not fill all the pore spaces of the cell membrane and so turgidity was minimal. Fig.11: Influence of gamma radiation on the resilience of mushrooms 4. CONCLUSION The Textural Profile Analysis results obtained for the parameters investigated for Pleurotus ostreatus revealed an apparent effect of gamma radiation on the fresh, dried and rehydrated states of the mushroom. Gamma radiation could however be used to prolong shelf life of mushrooms during storage. 5. ACKNOWLEDGEMENT Authors would like to express their gratitude to all technicians at the Department of Food Processing and Engineering, Food Research Institute, CSIR, Accra for their technical assistance. 6. REFERENCES [1] A.O.A.C. 1996. Official methods of analysis. Association of Official Analytical Chemists. Washington, D.C. [2] Amuthan, G., Visvanathan, R., Kailappan, R and Sreenarayanan, V. V.Studies on the osmo-air drying ofmilky mushroom, Calocybe indica. Mushroom Res., 8(2), 1999, 49-52. [3] Anonymous. Texture profile analysis. Available from < http:// www.texturetechnologies.com/texture_profile_analys is.html. 2005, Retrieved on 4/12/2012. [4] Ares, G, Lareo, C and Lema, P. Modified atmosphere packaging for postharvest storage of mushrooms. A review. Fresh Produce, 1(1), 2007, 32- 40. [5] Ares, G, Parentelli, C, Gambaro, A, Lareo, C and Lema, P. Sensory shelf life of shiitake mushrooms stored under passive modified atmosphere. Postharvest Biology and Technology, 41, 2006, 191- 197. [6] Argyropoulos, D, Heindel, A and Müller, J. Assessment of convection, hot-air combined with microwave-vacuum and freeze-drying methods for mushrooms with regard to product quality. Int. J. Food. Sci. Tech., 46(2), 2011, 333-342. [7] Arvanitoyannis, I.S. Legislation on food irradiation, In irradiation of food commodities, techniques, application, detection, legislation, safety and consumer opinion. Academic Press, 32 James Town Rd, London, NW1 7BY, UK. 2010. [8] Beelman, R.B, Frost, C.E and Guthrie, B. Evaluation of textural changes related to postharvest quality and shelflife of fresh mushrooms. In: Wuest PJ, Royse, D.J, Beelman, R.B (Eds) Cultivating Edible Fungi, Elsevier, Amsterdam, 1987, 251-258. [9] Bourne, M.C. Texture evaluation of horticultural crops, HortScience. 15, 1980, 51-56. [10] Bernas, E., Jaworska, G and Kmiecik, W. Storage and processing of edible mushrooms, ACTA Scientiarum Polonorum - Technologia Alimentaria 5(2), 2006, 5-23. [11] Casas, C., Martinez, O., Guillen, M. D., Pin, C. and Salmeron, J. Textural properties of raw Atlantic salmon (Salmo salar) at three points along the fillet, determined by different methods. Food Control, 17(7), 2006, 511-515. [12] Cavaco, A. M, Pinto, P, Antunes, M. D, Silva, J. M. and Guerra, R. Rocha pear firmness predicted by a Vis/NIR segmented model,” Postharvest Biol. Tec., 51, 2009, 311–319. [13] Gbedemah, C, Obodai, M and Sawyer, L.C. Preliminary investigations into the bioconversion of gamma irradiated agricultural wastes by Pleurotus spp. Radiation Phys.Chem. 52(6), 1998, 379-382. [14] Giri, S.K and Prasad, S. 2013. Quality characteristics of microwave-vacuum dried button mushrooms (Agaricus bisporus). Octa J. Biosciences, 1(1), 2013, 24-31. 0 0.5 1 1.5 2 0 0.5 1 1.5 2 Resilience Dose ( kGy ) Fresh Dried Rehydrated
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