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CURRICULUM VITAE
PERSONAL DETAILS:
Name: Muhammad Zoabi
Date of Birth: 16.02.1984
Address: Faculty of Biology, Technion-Israel Institute of Technology
Technion City, Haifa, 32000, Israel
Cell phone: +972-0503278858
Tel Lab: 972-4-8294231
Fax: 972-4-8513922
E-mail: zhamody@tx.technion.ac.il
hamoody.2001@gmail.com
EDUCATION:
Degrees:
2011-2016 PhD- Faculty of Biology, Technion-Israel Institute of Technology,
Haifa, Israel. Supervision of Dr.Nabieh Ayoub.
2008-2011 M.Sc.- - Faculty of Medicine. Technion-Israel Institute of Technology,
Haifa, Israel. Supervision of Prof. Aaron Ciechanover. Thesis: Proteins
of the PRC2 complex are degraded by the ubiquitin- proteasome
system.
2004-2007 B.A. - Faculty of Biology, Technion-Israel Institute of Technology,
Haifa, Israel.
2002 Graduated from ORT Ahva Naura High school
RESEARCH EXPERIENCE:
2011-Present In Dr. Nabieh Ayoub’s laboratory. I Study the biological function of
different members of KDM4 lysine demethylases.
2008-2010 In Prof. Aaron Ciechanover’s laboratory. I studied the degradation
mechanism of MyoD and PRC2 complex.
Teaching Experience:
10/2015-3/2016 Teacher assistant of Protein Biochemistry course. Faculty of
Biology, Technion-Israel Institute of Technology, Haifa, Israel.
03/2015-07/2015 Teacher assistant of Cell biology course. Faculty of Biology,
Technion-Israel Institute of Technology, Haifa, Israel.
10/2014-3/2015 Teacher assistant of Protein Biochemistry course. Faculty of
Biology, Technion-Israel Institute of Technology, Haifa, Israel.
03/2014-06/2014 Teacher assistant of Cell biology course. Faculty of Biology,
Technion-Israel Institute of Technology, Haifa, Israel.
10/2013-03/2014 Teacher assistant assistant of Molecular Genetics Lab. Faculty of
Biology, Technion-Israel Institute of Technology, Haifa, Israel.
03/2013-06/2013 Teacher assistant of Protein Biochemistry course. Faculty of
Biology, Technion-Israel Institute of Technology, Haifa, Israel.
10/2012- 3/2013 Teacher assistant of Molecular Genetics Lab. Faculty of Biology,
Technion-Israel Institute of Technology, Haifa, Israel.
PUBLICATIONS:
1. Sadeh R, Breitschopf K, Bercovich B, Zoabi M, Kravtsova-Ivantsiv Y, Kornitzer D,
Schwartz A, Ciechanover A. The N-terminal domain of MyoD is necessary and
sufficient for its nuclear localization-dependent degradation by the ubiquitin
system. Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15690-5.
2. Zoabi M, Sadeh R, de Bie P, Marquez VE, Ciechanover A.
PRAJA1 is a ubiquitin ligase for the polycomb repressive complex 2 proteins.
Biochem Biophys Res Commun. 2011 May 13;408(3):393-8.
3. Zoabi M, Nadar-Ponniah P, Khoury-Haddad H, Usaj M, Budowski-Tal I, Haran T,
Henn A, Mandel-Gutfreund Y, Ayoub N. RNA-dependent chromatin localization of
KDM4 lysine demethylase promotes H3K9me3 demethylation. Nucleic Acids
Research. 2014 Oct.
TECHNICAL EXPERTISE:
1. Molecular Biology Techniques: Gene cloning, plasmid and genomic DNA isolation,
plasmid transformation, site-directed mutagenesis, western blot and
Immunoprecipitation, cDNA synthesis, PCR, real-time PCR.
2. In vitro techniques: Demethylation assay, ubiquitination assay, In-vitro translation
,degradation assay, RNA radioactive labeling, binding assay, MST, EMSA
(Electrophoretic mobility shift assay).
3. Recombinant Protein Expression and Purification Systems: Bacterial and
mammalian.
4. Cell Culture Techniques: Maintenance of mammalian cell lines, transfections, gene
knock-down using shRNA and siRNA, establishment of stable cell lines, RNA
isolation, biochemical fractionation, Immunofluorescent staining of cells, Pulse-chase
labeling, generation of recombinant lentiviruses, RIP (RNA immunoprecipitation),
PAR-CLIP and iCLIP (crosslinking and immunoprecipitation, Ribosomal profiling,
Chromatin immunoprecipitation (ChIP).
5. Microscopic techniques: Confocal microscopy: FRAP (Fluorescent Recovery After
Photo bleaching), FLIP (Fluorescence loss after photobleaching).
6. Bioinformatics and computational techniques: BLAST, Multiple sequence alignment
(ClustalW), Clone manager Suite, Microsoft office (PowerPoint, Word, Excel, Visio),
Adobe Photoshop, Adobe Illustrator, Zen software, PyMOL.
Fellowships, Awards and Honors
January 2015- Excellence Scholarship from the Faculty of Biology, Technion.
January 2015- University Excellence in Teaching Award, Technion in cell biology course.
April 2014- Travel fellowship from Genomic Regulation Center (Barcelona) to participate
in Genome wide approaches to study functional protein-RNA interactions
course.
March 2013- Best Poster Presentation award from the faculty retreat, the Faculty of Biology,
Technion.
2006- Dean's list excellent student- Faculty of Biology, Technion.
Conferences and courses
1. Epigenetics - in dialogue with the genome (June, 2015), Our Dynamic Earth,
Edinburgh, Scotland.
2. Genome wide approaches to study functional protein-RNA interactions course (Sep
2014), CRG, Barcelona, Spain.
3. Genome stability conference (April 2014), Tel Aviv University, Israel.
4. The 7th FISEB (Ilanit) Congress (Feb 2014), Eilat, Israel.
5. First Israel-China (ISF-NSFC) Workshop on Genetics and Epigenetics of Human
Disease (Oct 2013), Jeruslem Israel.
6. DNA @ 60 (May 2013), Technion, Haifa, Israel
7. Genome stability conference (April 2012), Technion, Haifa, Israel
Languages
 English (very good)
 Hebrew (fluent)
 Arabic (mother tongue)

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Muhammad_Zoabi_CV 090216

  • 1. CURRICULUM VITAE PERSONAL DETAILS: Name: Muhammad Zoabi Date of Birth: 16.02.1984 Address: Faculty of Biology, Technion-Israel Institute of Technology Technion City, Haifa, 32000, Israel Cell phone: +972-0503278858 Tel Lab: 972-4-8294231 Fax: 972-4-8513922 E-mail: zhamody@tx.technion.ac.il hamoody.2001@gmail.com EDUCATION: Degrees: 2011-2016 PhD- Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel. Supervision of Dr.Nabieh Ayoub. 2008-2011 M.Sc.- - Faculty of Medicine. Technion-Israel Institute of Technology, Haifa, Israel. Supervision of Prof. Aaron Ciechanover. Thesis: Proteins of the PRC2 complex are degraded by the ubiquitin- proteasome system. 2004-2007 B.A. - Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel. 2002 Graduated from ORT Ahva Naura High school RESEARCH EXPERIENCE: 2011-Present In Dr. Nabieh Ayoub’s laboratory. I Study the biological function of different members of KDM4 lysine demethylases. 2008-2010 In Prof. Aaron Ciechanover’s laboratory. I studied the degradation mechanism of MyoD and PRC2 complex. Teaching Experience: 10/2015-3/2016 Teacher assistant of Protein Biochemistry course. Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel.
  • 2. 03/2015-07/2015 Teacher assistant of Cell biology course. Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel. 10/2014-3/2015 Teacher assistant of Protein Biochemistry course. Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel. 03/2014-06/2014 Teacher assistant of Cell biology course. Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel. 10/2013-03/2014 Teacher assistant assistant of Molecular Genetics Lab. Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel. 03/2013-06/2013 Teacher assistant of Protein Biochemistry course. Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel. 10/2012- 3/2013 Teacher assistant of Molecular Genetics Lab. Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel. PUBLICATIONS: 1. Sadeh R, Breitschopf K, Bercovich B, Zoabi M, Kravtsova-Ivantsiv Y, Kornitzer D, Schwartz A, Ciechanover A. The N-terminal domain of MyoD is necessary and sufficient for its nuclear localization-dependent degradation by the ubiquitin system. Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15690-5. 2. Zoabi M, Sadeh R, de Bie P, Marquez VE, Ciechanover A. PRAJA1 is a ubiquitin ligase for the polycomb repressive complex 2 proteins. Biochem Biophys Res Commun. 2011 May 13;408(3):393-8. 3. Zoabi M, Nadar-Ponniah P, Khoury-Haddad H, Usaj M, Budowski-Tal I, Haran T, Henn A, Mandel-Gutfreund Y, Ayoub N. RNA-dependent chromatin localization of KDM4 lysine demethylase promotes H3K9me3 demethylation. Nucleic Acids Research. 2014 Oct. TECHNICAL EXPERTISE: 1. Molecular Biology Techniques: Gene cloning, plasmid and genomic DNA isolation, plasmid transformation, site-directed mutagenesis, western blot and Immunoprecipitation, cDNA synthesis, PCR, real-time PCR. 2. In vitro techniques: Demethylation assay, ubiquitination assay, In-vitro translation
  • 3. ,degradation assay, RNA radioactive labeling, binding assay, MST, EMSA (Electrophoretic mobility shift assay). 3. Recombinant Protein Expression and Purification Systems: Bacterial and mammalian. 4. Cell Culture Techniques: Maintenance of mammalian cell lines, transfections, gene knock-down using shRNA and siRNA, establishment of stable cell lines, RNA isolation, biochemical fractionation, Immunofluorescent staining of cells, Pulse-chase labeling, generation of recombinant lentiviruses, RIP (RNA immunoprecipitation), PAR-CLIP and iCLIP (crosslinking and immunoprecipitation, Ribosomal profiling, Chromatin immunoprecipitation (ChIP). 5. Microscopic techniques: Confocal microscopy: FRAP (Fluorescent Recovery After Photo bleaching), FLIP (Fluorescence loss after photobleaching). 6. Bioinformatics and computational techniques: BLAST, Multiple sequence alignment (ClustalW), Clone manager Suite, Microsoft office (PowerPoint, Word, Excel, Visio), Adobe Photoshop, Adobe Illustrator, Zen software, PyMOL. Fellowships, Awards and Honors January 2015- Excellence Scholarship from the Faculty of Biology, Technion. January 2015- University Excellence in Teaching Award, Technion in cell biology course. April 2014- Travel fellowship from Genomic Regulation Center (Barcelona) to participate in Genome wide approaches to study functional protein-RNA interactions course. March 2013- Best Poster Presentation award from the faculty retreat, the Faculty of Biology, Technion. 2006- Dean's list excellent student- Faculty of Biology, Technion. Conferences and courses 1. Epigenetics - in dialogue with the genome (June, 2015), Our Dynamic Earth, Edinburgh, Scotland.
  • 4. 2. Genome wide approaches to study functional protein-RNA interactions course (Sep 2014), CRG, Barcelona, Spain. 3. Genome stability conference (April 2014), Tel Aviv University, Israel. 4. The 7th FISEB (Ilanit) Congress (Feb 2014), Eilat, Israel. 5. First Israel-China (ISF-NSFC) Workshop on Genetics and Epigenetics of Human Disease (Oct 2013), Jeruslem Israel. 6. DNA @ 60 (May 2013), Technion, Haifa, Israel 7. Genome stability conference (April 2012), Technion, Haifa, Israel Languages  English (very good)  Hebrew (fluent)  Arabic (mother tongue)