5. Hypothesis
• According to the conversion of
carbohydrates to lactic acid by the
streptococcus mutans (S.mutans) bacteria,
the beverage with the most sugar content
will cause the most damage to the tooth
enamel.
6. Objectives
• to determine which of the beverages
chosen is the one that causes the most
damage to the tooth enamel
• to establish if there is a relation between
sugar content and pH level in the erosion
of the tooth
• To understand why orthodontic
treatments forbid the consumption of
some beverages.
7. Materials
• Teeth
• Saliva
• Beverages
• Well plates
• Micropipettes
• Petri dishes
• pH meter
• Contact-stylus
surface
profilometer
• Optical 3D
microscope
Infinite Focus
8. Methodology
• Phase 1
– Petri dishes
• Phase 2
– Premeasures
– Dividing and labeling
– Saliva and beverages
– Add teeth
– Observe
– Measurements
– Comparison and analysis
9. Bibliography
– Vieira, A.R, Deelev, K.B, Callahan, N.F, and others; Detection of
Streptococcus mutans Genomic DNA in Human DNA Samples Extracted
from Saliva and Blood; ISRN Dent.; Published online 2011 April 10;
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128887/
– What lives inside your mouth?;
http://science.education.nih.gov/supplements/nih2/oral-health/guide/pdfs
– What acid is produced by bacteria?; Yahoo answers; 2010;
http://in.answers.yahoo.com/question/index?qid=20100622085725AAFMIL0
– Odontología UNA; Streptococcus mutans;
http://es.slideshare.net/dicefalo18/streptococcus-mutans
– The free dictionary; Dental biofilm; Farlex; 2008;
http://medical-dictionary.thefreedictionary.com/dental+biofilm
– Fonseca, G.; Esmalte Dental; UNIVERSIDAD PRIVADA ANTERNOR
ORREGO; http://www.scribd.com/doc/517866/ESMALTE-DENTAL
– The free dictionary; Caries; Farlex; 2007;
http://medical-dictionary.thefreedictionary.com/caries
10. – The free dictionary; Profilometer; Farlex; 2007, 2012;
http://medical-dictionary.thefreedictionary.com/profilometer
– Brook Anco; Focus Variation Systems; Brook Anco Corporation; 2013;
http://www.brookanco.com/Focus_Variation_Systems.html
– Vorburger, T.; Optical Methods of Surface Measurements; National
Institute of Standards and Technology (NIST); Measurement Science
and Standards in Forensic Firearms Analysis 2012 NIST, Gaithersburg,
MD; July 10, 2012 http://www.nist.gov/oles/upload/3-Vorburger-Ted-
Optical-Methods-of-Surface-Measurement-MSSFAA-10jul12.pdf
– Fujii, M.,Kitasako, M.,Sadr,A., Tagami,J.; Roughness and pH changes
of enamel surface induced by soft drinks in vitro-applications of stylus
profilometry, focus variation 3D scanning microscopy and micro pH
sensor; Department of Restorative Sciences, Tokyo Medical and
Dental University, Tokyo 113-8549, Japan; 2011;
http://lib.bioinfo.pl/pmid:21597209
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