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Microbial resources




               Mohamed Wahby
PowerPoint presentation as a partial fulfilment for
              the course Env. 704
     Pre. M.Sc. In environmental sciences
                   Dec. 2012
Contents
• Introduction
• In industry
• Microbial insecticides (Biological control)
• Water borne pathogens in developing
  countries
• Biodegradation
• Genetic engineering associated questioning
• Climatic change effect on microorganism’s
                Mohamed Wahby - Alexandria University -
                                                            2
                 Faculty of science - Microbial resources
Contents
• Introduction
• In industry
• Microbial insecticides (Biological control)
• Water borne pathogens in developing
  countries
• Biodegradation
• Genetic engineering associated questioning
• Climatic change effect on microorganism’s
                Mohamed Wahby - Alexandria University -
                                                            3
                 Faculty of science - Microbial resources
Microorganisms
• Microorganisms
  include
  bacteria, fungi,
  archaea or
  protists, but not
  viruses and
  prions, which
  are generally
  classified as
                                                 http://tbi.montana.edu/outreach/hotscience
  non-living.                                    /materials/resources/TreeofLife_TBI.jpg

http://www.sciencedaily.com/articles/m/microorganism.htm
                            Mohamed Wahby - Alexandria University -
                                                                                              4
                                  Faculty of science - Microbial resources
Microorganisms
• Harmful or beneficial?




http://meadfamilydental.com/wp-                     http://www.interactive-biology.com/wp-
content/uploads/2012/05/Photo-Mar-27-               content/uploads/2012/08/Probiotic-
12-45-02-AM.jpg                                     Yogurt-862x1024.jpg
                           Mohamed Wahby - Alexandria University -
                                                                                       5
                               Faculty of science - Microbial resources
Microorganisms
• Their role in nature: Carbon cycle




               Brock – Biology- of microorganisms – 13th edition - Chapter 24
                 Mohamed Wahby Alexandria University -
                                                                            6
                  Faculty of science - Microbial resources
Microorganisms
  • Their role in nature: Nitrogen cycle

http://www.eea.europa.e
u/data-and-
maps/figures/the-
nitrogen-cycle/trend10-
2s-soer2010-
eps/TREND10-2S-
nitrogen-
cycle.eps.75dpi.gif/image




                             Mohamed Wahby - Alexandria University -
                                                                         7
                              Faculty of science - Microbial resources
Microorganisms
  • Their role in nature: Nitrogen cycle


Brock – Biology of microorganisms –
13th edition - Chapter 24




                              Mohamed Wahby - Alexandria University -
                                                                          8
                               Faculty of science - Microbial resources
Microorganisms
• Their role in nature: Sulphur cycle




      http://img.docstoccdn.com/thumb/orig/120991179.png
                     Mohamed Wahby - Alexandria University -
                                                                 9
                      Faculty of science - Microbial resources
Microorganisms
  • Their role in nature: Sulphur cycle


Brock – Biology of microorganisms –
13th edition - Chapter 24




                              Mohamed Wahby - Alexandria University -
                                                                          10
                               Faculty of science - Microbial resources
Microorganisms
• Bacteria




 http://www.scilogs.com/import-data/images/11/bacteria.jpg
                         Mohamed Wahby - Alexandria University -
                                                                     11
                          Faculty of science - Microbial resources
Microorganisms

• Why bacteria?
• Oldest on earth
• Nothing works
  without them
• Basic and most
  studied
  organism
• Everywhere            http://tbi.montana.edu/outreach/hotscience
                        /materials/resources/TreeofLife_TBI.jpg
               Mohamed Wahby - Alexandria University -
                                                                     12
                Faculty of science - Microbial resources
Microorganisms
• Why bacteria genetically?
Simplicity and Polymerization rate




                      Mohamed Wahby - Alexandria University -
         http://barleyworld.org/sites/default/files/figure-08-01_0.jpg   13
                       Faculty of science - Microbial resources
Microorganisms
• Why bacteria physiologically?


 • Primary
 metabolites

 • Secondary
 metabolites


http://ebsbiowizard.com/wp-content/uploads/2010/11/bacterial-growth-curve.png
                           Mohamed Wahby - Alexandria University -
                                                                                14
                            Faculty of science - Microbial resources
Contents
• Introduction
• In industry
• Microbial insecticides (Biological control)
• Water borne pathogens in developing
  countries
• Biodegradation
• Genetic engineering associated questioning
• Climatic change effect on microorganism’s
                Mohamed Wahby - Alexandria University -
                                                            15
                 Faculty of science - Microbial resources
Production of antibiotics
 • Are produced from actinomycetes
 • Are naturals, semi-synthetics or synthetics
 • Classified according to their “mode of action”




http://www.armradio.am/en/wp-             http://muirbiology.files.wordpress.com/2012/1
content/uploads/2012/11/antibiotik.jpg - Alexandria University -
                            Mohamed Wahby 1/2012-11-01-14-41-22.jpg
                                                                                   16
                              Faculty of science - Microbial resources
Discovery


Sir Alexander Fleming
(National Library of Medicine
/Photo Researchers, Inc.)




  Image taken from: “Deadly diseases and epidemics, antibiotics-resistant bacteria”.
  Patrick G. Guilfoile, Ph.D. FOUNDING EDITOR, The Late I. Edward Alcamo.
  Distinguished Teaching Professor of Microbiology, SUNY Farmingdale. FOREWORD
  BY David Heymann. World Health Organization. Library of Congress Cataloging-in-
  Publication Data. ISBN: 978-0-7910-9188-3
                             Mohamed Wahby - Alexandria University -
                                                                                       17
                              Faculty of science - Microbial resources
Photograph of the original
culture plate of the fungus
Penicillium notatum made
by Sir Alexander Fleming (St.
Mary’s
Hospital Medical
School/Photo Researchers,
Inc.)


                    Mohamed Wahby - Alexandria University -
                                                                18
                     Faculty of science - Microbial resources
Production of antibiotics
  • Principle: Substitutability test (in lab)

• Bacterial susceptibility
  test. Practical section of
  course “General
  bacteriology ” Mic.311.
  Department of
  Microbiology, faculty of
  science, Alexandria
  University. Semester
  Autumn 2010.
                         Mohamed Wahby - Alexandria University -
                                                                     19
                          Faculty of science - Microbial resources
Production of vinegar
• Principle: Acetobacter


Okafor - Modern
industrial microbiology
and biotechnology –
Chapter 14 –
Production of vinegar


                   http://www.foodsketches.com/_media/_suppliers/_pics/10.jpg
                      Mohamed Wahby - Alexandria University -
                                                                         20
                       Faculty of science - Microbial resources
Production of single cell protein
  • Dairy wastes: Whey

    Okafor - Modern industrial
    microbiology and
    biotechnology – Chapter 15
    – Single cell protein


http://www.eplantscience.com/botanical_biot
echnology_biology_chemistry/biotechnology/
microbial_biotechnology/single_cell_protein_s
cp_and_mycoprotein/biotech_scp_production
_of_yeast_biomass.php
                               Mohamed Wahby - Alexandria University -
                                                                           21
                                Faculty of science - Microbial resources
Production of E162




http://www.ajinomoto.com/amino/eng/img/1_5_image.gif
               Mohamed Wahby - Alexandria University -
                                                           22
                Faculty of science - Microbial resources
Contents
• Introduction
• In industry
• Microbial insecticides (Biological control)
• Water borne pathogens in developing
  countries
• Biodegradation
• Genetic engineering associated questioning
• Climatic change effect on microorganism’s
                Mohamed Wahby - Alexandria University -
                                                            23
                 Faculty of science - Microbial resources
Microbial bio-control
• Bacillus thuringiensis


http://www.biocontrol.entomology.cor
nell.edu/pathogens/bacteria.html




                         Mohamed Wahby - Alexandria University -
                                                                     24
                          Faculty of science - Microbial resources
Contents
• Introduction
• In industry
• Microbial insecticides (Biological control)
• Water borne pathogens in developing
  countries
• Biodegradation
• Genetic engineering associated questioning
• Climatic change effect on microorganism’s
                Mohamed Wahby - Alexandria University -
                                                            25
                 Faculty of science - Microbial resources
Water borne diseases




http://www.righttoplay.com/International/news-and-media/PublishingImages/GOOD.png
                             Mohamed Wahby - Alexandria University -
                                                                               26
                            Faculty of science - Microbial resources
• Guidelines
  for drinking-
  water
  quality - 4th
  edition –
  WHO –
  Chapter 7,
  Microbial
  aspects
                  Mohamed Wahby - Alexandria University -
                                                              27
                   Faculty of science - Microbial resources
Contents
• Introduction
• In industry
• Microbial insecticides (Biological control)
• Water borne pathogens in developing
  countries
• Biodegradation
• Genetic engineering associated questioning
• Climatic change effect on microorganism’s
                Mohamed Wahby - Alexandria University -
                                                            28
                 Faculty of science - Microbial resources
Biodegradation of plastic




      Ralstonia eutropha                         http://techcrunch.com/2011/03/08/sodastream-
                                                 coca-cola-sustainable-packaging/

http://microbewiki.kenyon.edu/index.php/Rals
                               Mohamed Wahby - Alexandria University -
                                                                                           29
tonia_eutropha                  Faculty of science - Microbial resources
Mohamed Wahby - Alexandria University -
                                            30
 Faculty of science - Microbial resources
Effect of genetically modified plants on
           soil microorganisms
• “… Overall, the GM crops investigated so far seem to
  have little or no effect on soil microbial communities.
  The methods used, although suitable in different
  ways for the analysis of multiple sample numbers,
  were sensitive enough to detect changes due to
  environmental factors such as plant growth
  development, soil type, and year… ”
From Mitchell and Dong – environmental microbiology – 2nd
   edition – Chapter 10 - 2010 Wiley-Blackwell.


                    Mohamed Wahby - Alexandria University -
                                                                31
                     Faculty of science - Microbial resources
Biodegradation and phytoremediation




                                              http://nac.unl.edu/bufferguidelines/guidelines
                                              /3_productive_soils/5.html
http://newspaper.li/static/e9b7c973691655ac2
c84aecdae6a8dc5.jpg
                             Mohamed Wahby - Alexandria University -
                                                                                         32
                              Faculty of science - Microbial resources
Contents
• Introduction
• In industry
• Microbial insecticides (Biological control)
• Water borne pathogens in developing
  countries
• Biodegradation
• Genetic engineering associated questioning
• Climatic change effect on microorganism’s
                Mohamed Wahby - Alexandria University -
                                                            33
                 Faculty of science - Microbial resources
The effect of climatic change on
        microorganism’s




http://www.emlab.com/m/media/TempRanges_ER0807_Fig1.jpg
                Mohamed Wahby - Alexandria University -
                                                            34
                 Faculty of science - Microbial resources
In Egypt




http://www.narg.org.uk/images/egypt_satphoto_google.jpg
                 Mohamed Wahby - Alexandria University -
                                                             35
                  Faculty of science - Microbial resources
Conclusion
• Microorganisms are the main player in every
  process takes place on earth
• Microorganisms are an important (could be
  essential in some cases) source of food and
  products
• Every living being on earth is affected by
  climate change, even the unseen ones.


               Mohamed Wahby - Alexandria University -
                                                           36
                Faculty of science - Microbial resources
References
• Ralph Mitchell and Ji-Dong Gu – Environmental microbiology –
  2nd edition - Wiley-Blackwell, 2010
• Jacquelyn G. Black – Microbiology, principles and explortaions
  – 7th edition - Wiley-Blackwell, 2008
• Nduka Okafor – Modern industrial microbiology and
  biotechnology – Science publisher, 2007
• Brock – Biology of microorganisms – 13th edition - Pearson
  Education 2012, 2009, 2006
• Promila Parihar – Microbiology and immunology – Swastik
  Publisher & distributors, 2009
• Naill A. Logan – Bacterial systematic - Wiley-Blackwell, 1994
                     Mohamed Wahby - Alexandria University -
                                                                 37
                      Faculty of science - Microbial resources

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Microbial resources

  • 1. Microbial resources Mohamed Wahby PowerPoint presentation as a partial fulfilment for the course Env. 704 Pre. M.Sc. In environmental sciences Dec. 2012
  • 2. Contents • Introduction • In industry • Microbial insecticides (Biological control) • Water borne pathogens in developing countries • Biodegradation • Genetic engineering associated questioning • Climatic change effect on microorganism’s Mohamed Wahby - Alexandria University - 2 Faculty of science - Microbial resources
  • 3. Contents • Introduction • In industry • Microbial insecticides (Biological control) • Water borne pathogens in developing countries • Biodegradation • Genetic engineering associated questioning • Climatic change effect on microorganism’s Mohamed Wahby - Alexandria University - 3 Faculty of science - Microbial resources
  • 4. Microorganisms • Microorganisms include bacteria, fungi, archaea or protists, but not viruses and prions, which are generally classified as http://tbi.montana.edu/outreach/hotscience non-living. /materials/resources/TreeofLife_TBI.jpg http://www.sciencedaily.com/articles/m/microorganism.htm Mohamed Wahby - Alexandria University - 4 Faculty of science - Microbial resources
  • 5. Microorganisms • Harmful or beneficial? http://meadfamilydental.com/wp- http://www.interactive-biology.com/wp- content/uploads/2012/05/Photo-Mar-27- content/uploads/2012/08/Probiotic- 12-45-02-AM.jpg Yogurt-862x1024.jpg Mohamed Wahby - Alexandria University - 5 Faculty of science - Microbial resources
  • 6. Microorganisms • Their role in nature: Carbon cycle Brock – Biology- of microorganisms – 13th edition - Chapter 24 Mohamed Wahby Alexandria University - 6 Faculty of science - Microbial resources
  • 7. Microorganisms • Their role in nature: Nitrogen cycle http://www.eea.europa.e u/data-and- maps/figures/the- nitrogen-cycle/trend10- 2s-soer2010- eps/TREND10-2S- nitrogen- cycle.eps.75dpi.gif/image Mohamed Wahby - Alexandria University - 7 Faculty of science - Microbial resources
  • 8. Microorganisms • Their role in nature: Nitrogen cycle Brock – Biology of microorganisms – 13th edition - Chapter 24 Mohamed Wahby - Alexandria University - 8 Faculty of science - Microbial resources
  • 9. Microorganisms • Their role in nature: Sulphur cycle http://img.docstoccdn.com/thumb/orig/120991179.png Mohamed Wahby - Alexandria University - 9 Faculty of science - Microbial resources
  • 10. Microorganisms • Their role in nature: Sulphur cycle Brock – Biology of microorganisms – 13th edition - Chapter 24 Mohamed Wahby - Alexandria University - 10 Faculty of science - Microbial resources
  • 11. Microorganisms • Bacteria http://www.scilogs.com/import-data/images/11/bacteria.jpg Mohamed Wahby - Alexandria University - 11 Faculty of science - Microbial resources
  • 12. Microorganisms • Why bacteria? • Oldest on earth • Nothing works without them • Basic and most studied organism • Everywhere http://tbi.montana.edu/outreach/hotscience /materials/resources/TreeofLife_TBI.jpg Mohamed Wahby - Alexandria University - 12 Faculty of science - Microbial resources
  • 13. Microorganisms • Why bacteria genetically? Simplicity and Polymerization rate Mohamed Wahby - Alexandria University - http://barleyworld.org/sites/default/files/figure-08-01_0.jpg 13 Faculty of science - Microbial resources
  • 14. Microorganisms • Why bacteria physiologically? • Primary metabolites • Secondary metabolites http://ebsbiowizard.com/wp-content/uploads/2010/11/bacterial-growth-curve.png Mohamed Wahby - Alexandria University - 14 Faculty of science - Microbial resources
  • 15. Contents • Introduction • In industry • Microbial insecticides (Biological control) • Water borne pathogens in developing countries • Biodegradation • Genetic engineering associated questioning • Climatic change effect on microorganism’s Mohamed Wahby - Alexandria University - 15 Faculty of science - Microbial resources
  • 16. Production of antibiotics • Are produced from actinomycetes • Are naturals, semi-synthetics or synthetics • Classified according to their “mode of action” http://www.armradio.am/en/wp- http://muirbiology.files.wordpress.com/2012/1 content/uploads/2012/11/antibiotik.jpg - Alexandria University - Mohamed Wahby 1/2012-11-01-14-41-22.jpg 16 Faculty of science - Microbial resources
  • 17. Discovery Sir Alexander Fleming (National Library of Medicine /Photo Researchers, Inc.) Image taken from: “Deadly diseases and epidemics, antibiotics-resistant bacteria”. Patrick G. Guilfoile, Ph.D. FOUNDING EDITOR, The Late I. Edward Alcamo. Distinguished Teaching Professor of Microbiology, SUNY Farmingdale. FOREWORD BY David Heymann. World Health Organization. Library of Congress Cataloging-in- Publication Data. ISBN: 978-0-7910-9188-3 Mohamed Wahby - Alexandria University - 17 Faculty of science - Microbial resources
  • 18. Photograph of the original culture plate of the fungus Penicillium notatum made by Sir Alexander Fleming (St. Mary’s Hospital Medical School/Photo Researchers, Inc.) Mohamed Wahby - Alexandria University - 18 Faculty of science - Microbial resources
  • 19. Production of antibiotics • Principle: Substitutability test (in lab) • Bacterial susceptibility test. Practical section of course “General bacteriology ” Mic.311. Department of Microbiology, faculty of science, Alexandria University. Semester Autumn 2010. Mohamed Wahby - Alexandria University - 19 Faculty of science - Microbial resources
  • 20. Production of vinegar • Principle: Acetobacter Okafor - Modern industrial microbiology and biotechnology – Chapter 14 – Production of vinegar http://www.foodsketches.com/_media/_suppliers/_pics/10.jpg Mohamed Wahby - Alexandria University - 20 Faculty of science - Microbial resources
  • 21. Production of single cell protein • Dairy wastes: Whey Okafor - Modern industrial microbiology and biotechnology – Chapter 15 – Single cell protein http://www.eplantscience.com/botanical_biot echnology_biology_chemistry/biotechnology/ microbial_biotechnology/single_cell_protein_s cp_and_mycoprotein/biotech_scp_production _of_yeast_biomass.php Mohamed Wahby - Alexandria University - 21 Faculty of science - Microbial resources
  • 22. Production of E162 http://www.ajinomoto.com/amino/eng/img/1_5_image.gif Mohamed Wahby - Alexandria University - 22 Faculty of science - Microbial resources
  • 23. Contents • Introduction • In industry • Microbial insecticides (Biological control) • Water borne pathogens in developing countries • Biodegradation • Genetic engineering associated questioning • Climatic change effect on microorganism’s Mohamed Wahby - Alexandria University - 23 Faculty of science - Microbial resources
  • 24. Microbial bio-control • Bacillus thuringiensis http://www.biocontrol.entomology.cor nell.edu/pathogens/bacteria.html Mohamed Wahby - Alexandria University - 24 Faculty of science - Microbial resources
  • 25. Contents • Introduction • In industry • Microbial insecticides (Biological control) • Water borne pathogens in developing countries • Biodegradation • Genetic engineering associated questioning • Climatic change effect on microorganism’s Mohamed Wahby - Alexandria University - 25 Faculty of science - Microbial resources
  • 26. Water borne diseases http://www.righttoplay.com/International/news-and-media/PublishingImages/GOOD.png Mohamed Wahby - Alexandria University - 26 Faculty of science - Microbial resources
  • 27. • Guidelines for drinking- water quality - 4th edition – WHO – Chapter 7, Microbial aspects Mohamed Wahby - Alexandria University - 27 Faculty of science - Microbial resources
  • 28. Contents • Introduction • In industry • Microbial insecticides (Biological control) • Water borne pathogens in developing countries • Biodegradation • Genetic engineering associated questioning • Climatic change effect on microorganism’s Mohamed Wahby - Alexandria University - 28 Faculty of science - Microbial resources
  • 29. Biodegradation of plastic Ralstonia eutropha http://techcrunch.com/2011/03/08/sodastream- coca-cola-sustainable-packaging/ http://microbewiki.kenyon.edu/index.php/Rals Mohamed Wahby - Alexandria University - 29 tonia_eutropha Faculty of science - Microbial resources
  • 30. Mohamed Wahby - Alexandria University - 30 Faculty of science - Microbial resources
  • 31. Effect of genetically modified plants on soil microorganisms • “… Overall, the GM crops investigated so far seem to have little or no effect on soil microbial communities. The methods used, although suitable in different ways for the analysis of multiple sample numbers, were sensitive enough to detect changes due to environmental factors such as plant growth development, soil type, and year… ” From Mitchell and Dong – environmental microbiology – 2nd edition – Chapter 10 - 2010 Wiley-Blackwell. Mohamed Wahby - Alexandria University - 31 Faculty of science - Microbial resources
  • 32. Biodegradation and phytoremediation http://nac.unl.edu/bufferguidelines/guidelines /3_productive_soils/5.html http://newspaper.li/static/e9b7c973691655ac2 c84aecdae6a8dc5.jpg Mohamed Wahby - Alexandria University - 32 Faculty of science - Microbial resources
  • 33. Contents • Introduction • In industry • Microbial insecticides (Biological control) • Water borne pathogens in developing countries • Biodegradation • Genetic engineering associated questioning • Climatic change effect on microorganism’s Mohamed Wahby - Alexandria University - 33 Faculty of science - Microbial resources
  • 34. The effect of climatic change on microorganism’s http://www.emlab.com/m/media/TempRanges_ER0807_Fig1.jpg Mohamed Wahby - Alexandria University - 34 Faculty of science - Microbial resources
  • 35. In Egypt http://www.narg.org.uk/images/egypt_satphoto_google.jpg Mohamed Wahby - Alexandria University - 35 Faculty of science - Microbial resources
  • 36. Conclusion • Microorganisms are the main player in every process takes place on earth • Microorganisms are an important (could be essential in some cases) source of food and products • Every living being on earth is affected by climate change, even the unseen ones. Mohamed Wahby - Alexandria University - 36 Faculty of science - Microbial resources
  • 37. References • Ralph Mitchell and Ji-Dong Gu – Environmental microbiology – 2nd edition - Wiley-Blackwell, 2010 • Jacquelyn G. Black – Microbiology, principles and explortaions – 7th edition - Wiley-Blackwell, 2008 • Nduka Okafor – Modern industrial microbiology and biotechnology – Science publisher, 2007 • Brock – Biology of microorganisms – 13th edition - Pearson Education 2012, 2009, 2006 • Promila Parihar – Microbiology and immunology – Swastik Publisher & distributors, 2009 • Naill A. Logan – Bacterial systematic - Wiley-Blackwell, 1994 Mohamed Wahby - Alexandria University - 37 Faculty of science - Microbial resources