SlideShare ist ein Scribd-Unternehmen logo
1 von 6
Downloaden Sie, um offline zu lesen
Leonid Asipov vs
"Expression of cyanobacterial ictB in higher plants enhanced photosynthesis and growth"
Leonid Asipov, 2014
Summary
The aim of the original study(1) was to compare the photosynthetic properties of wt plants relative
to plants transgenic with ictB, protein which is considered to be involved in carbon transport in
cyanobacteria(2). The author of the original article have presented only partial data and exclusively
data which was convenient for his conclusions. The full data suggests no practical advantage for an
implementation of the transgenic protein in agriculture, since wt plants performed better at all the
"close to reality" CO2 concentration points(300-800 PPM).
For the sake of scientific truth, I will present the full data results and show how the author of the
original article has been misleading in his conclusions by presenting only partial data.
The original results : a single transgenic plant was presented
Fig 1: The original chart containing one transgenic plant vs an average of 7 wt plants.
Note that even the Y standard deviation of the wt plants was omitted.
The author has presented a single transgenic plant relative to average of 7 wt plants (Fig 1). Many
more transgenic plants were sampled and some of them were similar to wt, some few were better
and some were worse photosynthetically (Fig 2).
Fig 2: Presentation of all the data. Ci vs Photosynthesis curve. Dark green represents wt
plants.
Fig 3 : Average series of wt and transgenic plants. Ci vs Photosynthesis curve. Transgenic
N=31 wt N=7.
The wt plants are better at all points except the first three (50, 100 and 200 PPM). At 200 PPM,
there are no visible differences between wt and the transgenic plants.
In the following chart the photosynthetic properties of transgenic and wt plants has been statistically
compared. The T test was calculated for each [CO2] point separately.
Fig 4: Statistical T test of photosynthesis values at every [CO2] point separately.
In the first two points (50 and 100 PPM) the differences between wt and transgenic plants are
significant but meaningless since the photosynthesis value is very close to or less than 0.
The CO2 concentration on the first two points (50 and 100 PPM [CO2]) is insufficient for plants
growth(3) and therefore, the differences in the photosynthesis rate of plants are not practical.
At 300, 400, 600 and 800 PPM [CO2], wt plants photosynthesise significantly better than the
transgenic plants.
Fig 5 : Citations from the original article(1).
The author of the original article(1) claims that the transgenic plants photosynthesise better
"under limiting but not under saturating CO2 concentrations" and therefore the protein “can be used
as crop yield stimulator". To be precise, the only [CO2] point with positive photosynthesis at which
the transgenic plants are significantly better than wt is 100 PPM [CO2]. The average photosynthesis
rate of the transgenic plants at this point is 0.35 umol CO2 m-2 s-1, value which is too low for
healthy plant growth. At normal earth atmosphere CO2 concentration (around 400 PPM), wt plants
photosynthesise significantly better(Fig 4) and therefore there is no practical use
of the transgenic protein as "crop yield stimulator".
The plants growth experiments
Fig 6: RH of the chamber during the experiment. The RH is “low humidity” according to the
original article(Fig 7).
The authors' results suggest that at lower humidity, transgenic plants grow better than wt. Such
results are inconsistent with the gas exchange results, which report significantly better wt
photosynthesis at 400 PPM [CO2] (Fig 8), concentration at which all the plants were grown. Since,
the photosynthesis of wt plants is higher, the growth is expected to be as well.
Fig 7: Citation from the original article (1). “Low humidity” is considered to be less than
30%.
All the experiments were conducted at RH of less than 22% (Fig 6), value which is considered,
according to the article, as "low humidity" (1). According to the presented results, at such humidity
the transgenic plants grow better. Such declaration is not observed in the gas-exchange results
(Fig 2).
II Generation of plants
This part of the experiment was not published or mentioned in the original article. The best
transgenic plants were grown for another generation and sampled similarly to generation I.
Fig 7 : Ci vs Photosynthesis generation II. All the plants.
To compare the photosynthesis values only, an External [CO2] vs photosynthesis chart was built.
Fig 8: External [CO2] vs Photosynthesis curve of II generation.
Average series. Transgenic N=21 wt N=9.
The results suggest that there are no significant differences between the transgenic plants and wt.
Since the best transgenic plants did not pass their good properties to their progeny, I suggest that
their better properties at generation I were observed due to measurement inaccuracies and not due to
better genetics. Results of both generations suggest that wt plants photosynthesise equally to or
better than the transgenic at all points except 50 and 100 PPM [CO2]. Values which are too low for
normal plant growth.
Conclusions
The author of the original article has hidden data and presented false conclusions. The wt plants
photosynthesise better at the normal atmosphere [CO2] concentration (Fig 4 and Fig 7). This is the
reason why the transgenic protein is not practical for implementation in agriculture.
Materials and Methods
All measurements were made on LICOR LI6400 Portable Photosynthesis System.
The illumination levels were 500 uE. All the plants were well watered before measurement.
All the raw data of this article (LI6400 files) can be downloaded at :
http://www.ldata.co.il/resources/proteingasexchange2.zip
Citations
(1) Expression of cyanobacterial ictB in higher plants enhanced photosynthesis and growth.
Judy Lieman-Hurwitz, Leonid Asipov, Shimon Rachmilevitch, Yehouda Marcus, Aaron Kaplan.
Plant Responses to Air Pollution and Global Change, 2005, pp 133-139
(2) A putative HCO3- transporter in the cyanobacterium Synechococcus sp. strain PCC 7942.
D J Bonfil; M Ronen-Tarazi; D Sültemeyer; J Lieman-Hurwitz; D Schatz; A Kaplan
FEBS letters, volume 430. Jul 2008.
(3) Plant responses to low [CO2] of the past.
Laci M. Gerhart and Joy K. Ward
New Phytologist, volume 188 Issue 3, Sep 2010.

Weitere ähnliche Inhalte

Ähnlich wie asipov_vs_kaplan2_.pdf

IRJET- The Comparative Study of On-Site Bio-Composting and Natural Compos...
IRJET-  	  The Comparative Study of On-Site Bio-Composting and Natural Compos...IRJET-  	  The Comparative Study of On-Site Bio-Composting and Natural Compos...
IRJET- The Comparative Study of On-Site Bio-Composting and Natural Compos...IRJET Journal
 
Environmental Engineering for Enhancing the Suitability of a Microalga for En...
Environmental Engineering for Enhancing the Suitability of a Microalga for En...Environmental Engineering for Enhancing the Suitability of a Microalga for En...
Environmental Engineering for Enhancing the Suitability of a Microalga for En...IRJET Journal
 
Poster tomato IPAP 2018.pdf
Poster tomato IPAP 2018.pdfPoster tomato IPAP 2018.pdf
Poster tomato IPAP 2018.pdftachet
 
Acceleration of Lead Phytostabilization by Maize (Zea mays) in Association wi...
Acceleration of Lead Phytostabilization by Maize (Zea mays) in Association wi...Acceleration of Lead Phytostabilization by Maize (Zea mays) in Association wi...
Acceleration of Lead Phytostabilization by Maize (Zea mays) in Association wi...Agriculture Journal IJOEAR
 
International Journal of Engineering and Science Invention (IJESI)
International Journal of Engineering and Science Invention (IJESI) International Journal of Engineering and Science Invention (IJESI)
International Journal of Engineering and Science Invention (IJESI) inventionjournals
 
IRJET-Biogas Generation from Combination of Food Waste and Water Hyacinth
IRJET-Biogas Generation from Combination of Food Waste and Water HyacinthIRJET-Biogas Generation from Combination of Food Waste and Water Hyacinth
IRJET-Biogas Generation from Combination of Food Waste and Water HyacinthIRJET Journal
 
Anaerobic Digestion of Biodegradable Organics in Municipal Solid Wastes in Na...
Anaerobic Digestion of Biodegradable Organics in Municipal Solid Wastes in Na...Anaerobic Digestion of Biodegradable Organics in Municipal Solid Wastes in Na...
Anaerobic Digestion of Biodegradable Organics in Municipal Solid Wastes in Na...IRJET Journal
 
Effect of CO2 on enzymatic ectivity, secondary metabolites and expression of ...
Effect of CO2 on enzymatic ectivity, secondary metabolites and expression of ...Effect of CO2 on enzymatic ectivity, secondary metabolites and expression of ...
Effect of CO2 on enzymatic ectivity, secondary metabolites and expression of ...ICRISAT
 
High-throughput Miniaturized Bioreactors for Cell Culture Process Developmen...
High-throughput Miniaturized Bioreactors for Cell Culture  Process Developmen...High-throughput Miniaturized Bioreactors for Cell Culture  Process Developmen...
High-throughput Miniaturized Bioreactors for Cell Culture Process Developmen...KBI Biopharma
 
IRJET - Effects of Iron Oxide Nanoparticles on Chick Pea (Cicer Arietinum): P...
IRJET - Effects of Iron Oxide Nanoparticles on Chick Pea (Cicer Arietinum): P...IRJET - Effects of Iron Oxide Nanoparticles on Chick Pea (Cicer Arietinum): P...
IRJET - Effects of Iron Oxide Nanoparticles on Chick Pea (Cicer Arietinum): P...IRJET Journal
 
ScienceCriticism.pdf
ScienceCriticism.pdfScienceCriticism.pdf
ScienceCriticism.pdfLeonid Ledata
 
ScienceCriticism.pdf
ScienceCriticism.pdfScienceCriticism.pdf
ScienceCriticism.pdfLeonid Ledata
 
FEASIBILITY STUDY OF TREATMENT OF EFFLUENT FROM A BULK DRUG MANUFACTURING IND...
FEASIBILITY STUDY OF TREATMENT OF EFFLUENT FROM A BULK DRUG MANUFACTURING IND...FEASIBILITY STUDY OF TREATMENT OF EFFLUENT FROM A BULK DRUG MANUFACTURING IND...
FEASIBILITY STUDY OF TREATMENT OF EFFLUENT FROM A BULK DRUG MANUFACTURING IND...Journal For Research
 
Using an algal photo bioreactor as a polishing step for secondary treated was...
Using an algal photo bioreactor as a polishing step for secondary treated was...Using an algal photo bioreactor as a polishing step for secondary treated was...
Using an algal photo bioreactor as a polishing step for secondary treated was...AhmedKaram55
 
IRJET- Methanogenesis of Prosopis Juliflora
IRJET-  	  Methanogenesis of Prosopis JulifloraIRJET-  	  Methanogenesis of Prosopis Juliflora
IRJET- Methanogenesis of Prosopis JulifloraIRJET Journal
 
protease activity of extracellular enzyme produced by b. subtilis isolated fr...
protease activity of extracellular enzyme produced by b. subtilis isolated fr...protease activity of extracellular enzyme produced by b. subtilis isolated fr...
protease activity of extracellular enzyme produced by b. subtilis isolated fr...IJEAB
 
Alteration in photosynthetic pigments, antioxidant enzymes and protein profil...
Alteration in photosynthetic pigments, antioxidant enzymes and protein profil...Alteration in photosynthetic pigments, antioxidant enzymes and protein profil...
Alteration in photosynthetic pigments, antioxidant enzymes and protein profil...Divya Srivastava
 

Ähnlich wie asipov_vs_kaplan2_.pdf (20)

IRJET- The Comparative Study of On-Site Bio-Composting and Natural Compos...
IRJET-  	  The Comparative Study of On-Site Bio-Composting and Natural Compos...IRJET-  	  The Comparative Study of On-Site Bio-Composting and Natural Compos...
IRJET- The Comparative Study of On-Site Bio-Composting and Natural Compos...
 
Environmental Engineering for Enhancing the Suitability of a Microalga for En...
Environmental Engineering for Enhancing the Suitability of a Microalga for En...Environmental Engineering for Enhancing the Suitability of a Microalga for En...
Environmental Engineering for Enhancing the Suitability of a Microalga for En...
 
Poster tomato IPAP 2018.pdf
Poster tomato IPAP 2018.pdfPoster tomato IPAP 2018.pdf
Poster tomato IPAP 2018.pdf
 
Acceleration of Lead Phytostabilization by Maize (Zea mays) in Association wi...
Acceleration of Lead Phytostabilization by Maize (Zea mays) in Association wi...Acceleration of Lead Phytostabilization by Maize (Zea mays) in Association wi...
Acceleration of Lead Phytostabilization by Maize (Zea mays) in Association wi...
 
International Journal of Engineering and Science Invention (IJESI)
International Journal of Engineering and Science Invention (IJESI) International Journal of Engineering and Science Invention (IJESI)
International Journal of Engineering and Science Invention (IJESI)
 
IRJET-Biogas Generation from Combination of Food Waste and Water Hyacinth
IRJET-Biogas Generation from Combination of Food Waste and Water HyacinthIRJET-Biogas Generation from Combination of Food Waste and Water Hyacinth
IRJET-Biogas Generation from Combination of Food Waste and Water Hyacinth
 
Be 4101 project
Be 4101 projectBe 4101 project
Be 4101 project
 
Anaerobic Digestion of Biodegradable Organics in Municipal Solid Wastes in Na...
Anaerobic Digestion of Biodegradable Organics in Municipal Solid Wastes in Na...Anaerobic Digestion of Biodegradable Organics in Municipal Solid Wastes in Na...
Anaerobic Digestion of Biodegradable Organics in Municipal Solid Wastes in Na...
 
Effect of CO2 on enzymatic ectivity, secondary metabolites and expression of ...
Effect of CO2 on enzymatic ectivity, secondary metabolites and expression of ...Effect of CO2 on enzymatic ectivity, secondary metabolites and expression of ...
Effect of CO2 on enzymatic ectivity, secondary metabolites and expression of ...
 
High-throughput Miniaturized Bioreactors for Cell Culture Process Developmen...
High-throughput Miniaturized Bioreactors for Cell Culture  Process Developmen...High-throughput Miniaturized Bioreactors for Cell Culture  Process Developmen...
High-throughput Miniaturized Bioreactors for Cell Culture Process Developmen...
 
IRJET - Effects of Iron Oxide Nanoparticles on Chick Pea (Cicer Arietinum): P...
IRJET - Effects of Iron Oxide Nanoparticles on Chick Pea (Cicer Arietinum): P...IRJET - Effects of Iron Oxide Nanoparticles on Chick Pea (Cicer Arietinum): P...
IRJET - Effects of Iron Oxide Nanoparticles on Chick Pea (Cicer Arietinum): P...
 
ScienceCriticism.pdf
ScienceCriticism.pdfScienceCriticism.pdf
ScienceCriticism.pdf
 
ScienceCriticism.pdf
ScienceCriticism.pdfScienceCriticism.pdf
ScienceCriticism.pdf
 
Science criticism
Science criticismScience criticism
Science criticism
 
FEASIBILITY STUDY OF TREATMENT OF EFFLUENT FROM A BULK DRUG MANUFACTURING IND...
FEASIBILITY STUDY OF TREATMENT OF EFFLUENT FROM A BULK DRUG MANUFACTURING IND...FEASIBILITY STUDY OF TREATMENT OF EFFLUENT FROM A BULK DRUG MANUFACTURING IND...
FEASIBILITY STUDY OF TREATMENT OF EFFLUENT FROM A BULK DRUG MANUFACTURING IND...
 
Using an algal photo bioreactor as a polishing step for secondary treated was...
Using an algal photo bioreactor as a polishing step for secondary treated was...Using an algal photo bioreactor as a polishing step for secondary treated was...
Using an algal photo bioreactor as a polishing step for secondary treated was...
 
IRJET- Methanogenesis of Prosopis Juliflora
IRJET-  	  Methanogenesis of Prosopis JulifloraIRJET-  	  Methanogenesis of Prosopis Juliflora
IRJET- Methanogenesis of Prosopis Juliflora
 
protease activity of extracellular enzyme produced by b. subtilis isolated fr...
protease activity of extracellular enzyme produced by b. subtilis isolated fr...protease activity of extracellular enzyme produced by b. subtilis isolated fr...
protease activity of extracellular enzyme produced by b. subtilis isolated fr...
 
Alteration in photosynthetic pigments, antioxidant enzymes and protein profil...
Alteration in photosynthetic pigments, antioxidant enzymes and protein profil...Alteration in photosynthetic pigments, antioxidant enzymes and protein profil...
Alteration in photosynthetic pigments, antioxidant enzymes and protein profil...
 
JBEI Highlights June 2020
JBEI Highlights June 2020JBEI Highlights June 2020
JBEI Highlights June 2020
 

Mehr von Leonid Ledata

мышонок11.pdf the little mouth mouse the little baby which havo of being born...
мышонок11.pdf the little mouth mouse the little baby which havo of being born...мышонок11.pdf the little mouth mouse the little baby which havo of being born...
мышонок11.pdf the little mouth mouse the little baby which havo of being born...Leonid Ledata
 
мышонок1.pdf мой второй сын мышонок который должен родится
мышонок1.pdf мой второй сын мышонок который должен родитсямышонок1.pdf мой второй сын мышонок который должен родится
мышонок1.pdf мой второй сын мышонок который должен родитсяLeonid Ledata
 
мышонок the second boy of leonid ledata the writer
мышонок the second boy of leonid ledata the writerмышонок the second boy of leonid ledata the writer
мышонок the second boy of leonid ledata the writerLeonid Ledata
 
T ВЫСШИЙ ЧИН BIGBADEND the knig of the 2024 leonid ledata
T ВЫСШИЙ ЧИН BIGBADEND the knig of the 2024 leonid ledataT ВЫСШИЙ ЧИН BIGBADEND the knig of the 2024 leonid ledata
T ВЫСШИЙ ЧИН BIGBADEND the knig of the 2024 leonid ledataLeonid Ledata
 
T ВЫСШИЙ ЧИН BIGBADEND the rank knig when rand bigbadend*NT
T ВЫСШИЙ ЧИН BIGBADEND the rank knig when rand bigbadend*NTT ВЫСШИЙ ЧИН BIGBADEND the rank knig when rand bigbadend*NT
T ВЫСШИЙ ЧИН BIGBADEND the rank knig when rand bigbadend*NTLeonid Ledata
 
T ВЫСШИЙ ЧИН BIGBADEND*N*T BIGBADEDND OF THE "RANDKING"
T ВЫСШИЙ ЧИН BIGBADEND*N*T BIGBADEDND OF THE "RANDKING"T ВЫСШИЙ ЧИН BIGBADEND*N*T BIGBADEDND OF THE "RANDKING"
T ВЫСШИЙ ЧИН BIGBADEND*N*T BIGBADEDND OF THE "RANDKING"Leonid Ledata
 
T ВЫСШИЙ ЧИН BIGBADEND *N*T don't sin לא חטייה sin every contradiction
T ВЫСШИЙ ЧИН BIGBADEND *N*T don't sin לא חטייה sin every contradictionT ВЫСШИЙ ЧИН BIGBADEND *N*T don't sin לא חטייה sin every contradiction
T ВЫСШИЙ ЧИН BIGBADEND *N*T don't sin לא חטייה sin every contradictionLeonid Ledata
 
T ВЫСШИЙ ЧИН BIGBADEND the ranking knig the top degree the foreigngate
T ВЫСШИЙ ЧИН BIGBADEND the ranking knig the top degree the foreigngateT ВЫСШИЙ ЧИН BIGBADEND the ranking knig the top degree the foreigngate
T ВЫСШИЙ ЧИН BIGBADEND the ranking knig the top degree the foreigngateLeonid Ledata
 
T ВЫСШИЙ ЧИН BIGBADEND the knig of the ranking
T ВЫСШИЙ ЧИН BIGBADEND the knig of the rankingT ВЫСШИЙ ЧИН BIGBADEND the knig of the ranking
T ВЫСШИЙ ЧИН BIGBADEND the knig of the rankingLeonid Ledata
 
TGUVA I have w wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww
TGUVA I have w wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwTGUVA I have w wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww
TGUVA I have w wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwLeonid Ledata
 
TGUVA LDATA N ETAT OF ISVAEL THE SHVAL THE MOUNTAIN OF BAGAE
TGUVA LDATA N ETAT OF ISVAEL THE SHVAL THE MOUNTAIN OF BAGAETGUVA LDATA N ETAT OF ISVAEL THE SHVAL THE MOUNTAIN OF BAGAE
TGUVA LDATA N ETAT OF ISVAEL THE SHVAL THE MOUNTAIN OF BAGAELeonid Ledata
 
T ВЫСШИЙ ЧИН BIGBADEND *N T THE "RANKING KING"
T ВЫСШИЙ ЧИН BIGBADEND *N T THE "RANKING KING"T ВЫСШИЙ ЧИН BIGBADEND *N T THE "RANKING KING"
T ВЫСШИЙ ЧИН BIGBADEND *N T THE "RANKING KING"Leonid Ledata
 
T ВЫСШИЙ ЧИН BIGBADEND11111111111111 THE BEASTS OF FLATTER OF KKKPLAN ARON AN...
T ВЫСШИЙ ЧИН BIGBADEND11111111111111 THE BEASTS OF FLATTER OF KKKPLAN ARON AN...T ВЫСШИЙ ЧИН BIGBADEND11111111111111 THE BEASTS OF FLATTER OF KKKPLAN ARON AN...
T ВЫСШИЙ ЧИН BIGBADEND11111111111111 THE BEASTS OF FLATTER OF KKKPLAN ARON AN...Leonid Ledata
 
MAHASH_11_4_2022_THE_ETATA_ATTITUDED_ME_AS_AN_ENEMY COPEA INTERROGATION SQUAD...
MAHASH_11_4_2022_THE_ETATA_ATTITUDED_ME_AS_AN_ENEMY COPEA INTERROGATION SQUAD...MAHASH_11_4_2022_THE_ETATA_ATTITUDED_ME_AS_AN_ENEMY COPEA INTERROGATION SQUAD...
MAHASH_11_4_2022_THE_ETATA_ATTITUDED_ME_AS_AN_ENEMY COPEA INTERROGATION SQUAD...Leonid Ledata
 
T ВЫСШИЙ ЧИН BIGBADEND THE END OF OLD IS DEATH
T ВЫСШИЙ ЧИН BIGBADEND THE END OF OLD IS DEATHT ВЫСШИЙ ЧИН BIGBADEND THE END OF OLD IS DEATH
T ВЫСШИЙ ЧИН BIGBADEND THE END OF OLD IS DEATHLeonid Ledata
 
T ВЫСШИЙ ЧИН BIGBADEND 19/3/2024 the as usual last knig
T ВЫСШИЙ ЧИН BIGBADEND 19/3/2024 the as usual last knigT ВЫСШИЙ ЧИН BIGBADEND 19/3/2024 the as usual last knig
T ВЫСШИЙ ЧИН BIGBADEND 19/3/2024 the as usual last knigLeonid Ledata
 
T ВЫСШИЙ ЧИН BIGBADEND the knig of the "ranking" the antypogona
T ВЫСШИЙ ЧИН BIGBADEND the knig of the "ranking" the antypogonaT ВЫСШИЙ ЧИН BIGBADEND the knig of the "ranking" the antypogona
T ВЫСШИЙ ЧИН BIGBADEND the knig of the "ranking" the antypogonaLeonid Ledata
 
LDATA_N_NETANYATTY THE IMPORT OF MURYKANYAN "POLICY" IS CONDEMNED
LDATA_N_NETANYATTY THE IMPORT OF MURYKANYAN "POLICY" IS CONDEMNEDLDATA_N_NETANYATTY THE IMPORT OF MURYKANYAN "POLICY" IS CONDEMNED
LDATA_N_NETANYATTY THE IMPORT OF MURYKANYAN "POLICY" IS CONDEMNEDLeonid Ledata
 
T ВЫСШИЙ ЧИН BIGBADEND THE KNIG OF THE CHIN THE ROAN*MONARCHYST
T ВЫСШИЙ ЧИН BIGBADEND THE KNIG OF THE CHIN THE ROAN*MONARCHYSTT ВЫСШИЙ ЧИН BIGBADEND THE KNIG OF THE CHIN THE ROAN*MONARCHYST
T ВЫСШИЙ ЧИН BIGBADEND THE KNIG OF THE CHIN THE ROAN*MONARCHYSTLeonid Ledata
 
T ВЫСШИЙ ЧИН BIGBADEND BIGBADEND*N*T LEONID LEDATA
T ВЫСШИЙ ЧИН BIGBADEND BIGBADEND*N*T LEONID LEDATAT ВЫСШИЙ ЧИН BIGBADEND BIGBADEND*N*T LEONID LEDATA
T ВЫСШИЙ ЧИН BIGBADEND BIGBADEND*N*T LEONID LEDATALeonid Ledata
 

Mehr von Leonid Ledata (20)

мышонок11.pdf the little mouth mouse the little baby which havo of being born...
мышонок11.pdf the little mouth mouse the little baby which havo of being born...мышонок11.pdf the little mouth mouse the little baby which havo of being born...
мышонок11.pdf the little mouth mouse the little baby which havo of being born...
 
мышонок1.pdf мой второй сын мышонок который должен родится
мышонок1.pdf мой второй сын мышонок который должен родитсямышонок1.pdf мой второй сын мышонок который должен родится
мышонок1.pdf мой второй сын мышонок который должен родится
 
мышонок the second boy of leonid ledata the writer
мышонок the second boy of leonid ledata the writerмышонок the second boy of leonid ledata the writer
мышонок the second boy of leonid ledata the writer
 
T ВЫСШИЙ ЧИН BIGBADEND the knig of the 2024 leonid ledata
T ВЫСШИЙ ЧИН BIGBADEND the knig of the 2024 leonid ledataT ВЫСШИЙ ЧИН BIGBADEND the knig of the 2024 leonid ledata
T ВЫСШИЙ ЧИН BIGBADEND the knig of the 2024 leonid ledata
 
T ВЫСШИЙ ЧИН BIGBADEND the rank knig when rand bigbadend*NT
T ВЫСШИЙ ЧИН BIGBADEND the rank knig when rand bigbadend*NTT ВЫСШИЙ ЧИН BIGBADEND the rank knig when rand bigbadend*NT
T ВЫСШИЙ ЧИН BIGBADEND the rank knig when rand bigbadend*NT
 
T ВЫСШИЙ ЧИН BIGBADEND*N*T BIGBADEDND OF THE "RANDKING"
T ВЫСШИЙ ЧИН BIGBADEND*N*T BIGBADEDND OF THE "RANDKING"T ВЫСШИЙ ЧИН BIGBADEND*N*T BIGBADEDND OF THE "RANDKING"
T ВЫСШИЙ ЧИН BIGBADEND*N*T BIGBADEDND OF THE "RANDKING"
 
T ВЫСШИЙ ЧИН BIGBADEND *N*T don't sin לא חטייה sin every contradiction
T ВЫСШИЙ ЧИН BIGBADEND *N*T don't sin לא חטייה sin every contradictionT ВЫСШИЙ ЧИН BIGBADEND *N*T don't sin לא חטייה sin every contradiction
T ВЫСШИЙ ЧИН BIGBADEND *N*T don't sin לא חטייה sin every contradiction
 
T ВЫСШИЙ ЧИН BIGBADEND the ranking knig the top degree the foreigngate
T ВЫСШИЙ ЧИН BIGBADEND the ranking knig the top degree the foreigngateT ВЫСШИЙ ЧИН BIGBADEND the ranking knig the top degree the foreigngate
T ВЫСШИЙ ЧИН BIGBADEND the ranking knig the top degree the foreigngate
 
T ВЫСШИЙ ЧИН BIGBADEND the knig of the ranking
T ВЫСШИЙ ЧИН BIGBADEND the knig of the rankingT ВЫСШИЙ ЧИН BIGBADEND the knig of the ranking
T ВЫСШИЙ ЧИН BIGBADEND the knig of the ranking
 
TGUVA I have w wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww
TGUVA I have w wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwTGUVA I have w wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww
TGUVA I have w wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww
 
TGUVA LDATA N ETAT OF ISVAEL THE SHVAL THE MOUNTAIN OF BAGAE
TGUVA LDATA N ETAT OF ISVAEL THE SHVAL THE MOUNTAIN OF BAGAETGUVA LDATA N ETAT OF ISVAEL THE SHVAL THE MOUNTAIN OF BAGAE
TGUVA LDATA N ETAT OF ISVAEL THE SHVAL THE MOUNTAIN OF BAGAE
 
T ВЫСШИЙ ЧИН BIGBADEND *N T THE "RANKING KING"
T ВЫСШИЙ ЧИН BIGBADEND *N T THE "RANKING KING"T ВЫСШИЙ ЧИН BIGBADEND *N T THE "RANKING KING"
T ВЫСШИЙ ЧИН BIGBADEND *N T THE "RANKING KING"
 
T ВЫСШИЙ ЧИН BIGBADEND11111111111111 THE BEASTS OF FLATTER OF KKKPLAN ARON AN...
T ВЫСШИЙ ЧИН BIGBADEND11111111111111 THE BEASTS OF FLATTER OF KKKPLAN ARON AN...T ВЫСШИЙ ЧИН BIGBADEND11111111111111 THE BEASTS OF FLATTER OF KKKPLAN ARON AN...
T ВЫСШИЙ ЧИН BIGBADEND11111111111111 THE BEASTS OF FLATTER OF KKKPLAN ARON AN...
 
MAHASH_11_4_2022_THE_ETATA_ATTITUDED_ME_AS_AN_ENEMY COPEA INTERROGATION SQUAD...
MAHASH_11_4_2022_THE_ETATA_ATTITUDED_ME_AS_AN_ENEMY COPEA INTERROGATION SQUAD...MAHASH_11_4_2022_THE_ETATA_ATTITUDED_ME_AS_AN_ENEMY COPEA INTERROGATION SQUAD...
MAHASH_11_4_2022_THE_ETATA_ATTITUDED_ME_AS_AN_ENEMY COPEA INTERROGATION SQUAD...
 
T ВЫСШИЙ ЧИН BIGBADEND THE END OF OLD IS DEATH
T ВЫСШИЙ ЧИН BIGBADEND THE END OF OLD IS DEATHT ВЫСШИЙ ЧИН BIGBADEND THE END OF OLD IS DEATH
T ВЫСШИЙ ЧИН BIGBADEND THE END OF OLD IS DEATH
 
T ВЫСШИЙ ЧИН BIGBADEND 19/3/2024 the as usual last knig
T ВЫСШИЙ ЧИН BIGBADEND 19/3/2024 the as usual last knigT ВЫСШИЙ ЧИН BIGBADEND 19/3/2024 the as usual last knig
T ВЫСШИЙ ЧИН BIGBADEND 19/3/2024 the as usual last knig
 
T ВЫСШИЙ ЧИН BIGBADEND the knig of the "ranking" the antypogona
T ВЫСШИЙ ЧИН BIGBADEND the knig of the "ranking" the antypogonaT ВЫСШИЙ ЧИН BIGBADEND the knig of the "ranking" the antypogona
T ВЫСШИЙ ЧИН BIGBADEND the knig of the "ranking" the antypogona
 
LDATA_N_NETANYATTY THE IMPORT OF MURYKANYAN "POLICY" IS CONDEMNED
LDATA_N_NETANYATTY THE IMPORT OF MURYKANYAN "POLICY" IS CONDEMNEDLDATA_N_NETANYATTY THE IMPORT OF MURYKANYAN "POLICY" IS CONDEMNED
LDATA_N_NETANYATTY THE IMPORT OF MURYKANYAN "POLICY" IS CONDEMNED
 
T ВЫСШИЙ ЧИН BIGBADEND THE KNIG OF THE CHIN THE ROAN*MONARCHYST
T ВЫСШИЙ ЧИН BIGBADEND THE KNIG OF THE CHIN THE ROAN*MONARCHYSTT ВЫСШИЙ ЧИН BIGBADEND THE KNIG OF THE CHIN THE ROAN*MONARCHYST
T ВЫСШИЙ ЧИН BIGBADEND THE KNIG OF THE CHIN THE ROAN*MONARCHYST
 
T ВЫСШИЙ ЧИН BIGBADEND BIGBADEND*N*T LEONID LEDATA
T ВЫСШИЙ ЧИН BIGBADEND BIGBADEND*N*T LEONID LEDATAT ВЫСШИЙ ЧИН BIGBADEND BIGBADEND*N*T LEONID LEDATA
T ВЫСШИЙ ЧИН BIGBADEND BIGBADEND*N*T LEONID LEDATA
 

Kürzlich hochgeladen

How to do quick user assign in kanban in Odoo 17 ERP
How to do quick user assign in kanban in Odoo 17 ERPHow to do quick user assign in kanban in Odoo 17 ERP
How to do quick user assign in kanban in Odoo 17 ERPCeline George
 
INTRODUCTION TO CATHOLIC CHRISTOLOGY.pptx
INTRODUCTION TO CATHOLIC CHRISTOLOGY.pptxINTRODUCTION TO CATHOLIC CHRISTOLOGY.pptx
INTRODUCTION TO CATHOLIC CHRISTOLOGY.pptxHumphrey A Beña
 
Food processing presentation for bsc agriculture hons
Food processing presentation for bsc agriculture honsFood processing presentation for bsc agriculture hons
Food processing presentation for bsc agriculture honsManeerUddin
 
Earth Day Presentation wow hello nice great
Earth Day Presentation wow hello nice greatEarth Day Presentation wow hello nice great
Earth Day Presentation wow hello nice greatYousafMalik24
 
Concurrency Control in Database Management system
Concurrency Control in Database Management systemConcurrency Control in Database Management system
Concurrency Control in Database Management systemChristalin Nelson
 
4.16.24 Poverty and Precarity--Desmond.pptx
4.16.24 Poverty and Precarity--Desmond.pptx4.16.24 Poverty and Precarity--Desmond.pptx
4.16.24 Poverty and Precarity--Desmond.pptxmary850239
 
ICS2208 Lecture6 Notes for SL spaces.pdf
ICS2208 Lecture6 Notes for SL spaces.pdfICS2208 Lecture6 Notes for SL spaces.pdf
ICS2208 Lecture6 Notes for SL spaces.pdfVanessa Camilleri
 
ROLES IN A STAGE PRODUCTION in arts.pptx
ROLES IN A STAGE PRODUCTION in arts.pptxROLES IN A STAGE PRODUCTION in arts.pptx
ROLES IN A STAGE PRODUCTION in arts.pptxVanesaIglesias10
 
4.18.24 Movement Legacies, Reflection, and Review.pptx
4.18.24 Movement Legacies, Reflection, and Review.pptx4.18.24 Movement Legacies, Reflection, and Review.pptx
4.18.24 Movement Legacies, Reflection, and Review.pptxmary850239
 
Keynote by Prof. Wurzer at Nordex about IP-design
Keynote by Prof. Wurzer at Nordex about IP-designKeynote by Prof. Wurzer at Nordex about IP-design
Keynote by Prof. Wurzer at Nordex about IP-designMIPLM
 
MULTIDISCIPLINRY NATURE OF THE ENVIRONMENTAL STUDIES.pptx
MULTIDISCIPLINRY NATURE OF THE ENVIRONMENTAL STUDIES.pptxMULTIDISCIPLINRY NATURE OF THE ENVIRONMENTAL STUDIES.pptx
MULTIDISCIPLINRY NATURE OF THE ENVIRONMENTAL STUDIES.pptxAnupkumar Sharma
 
ANG SEKTOR NG agrikultura.pptx QUARTER 4
ANG SEKTOR NG agrikultura.pptx QUARTER 4ANG SEKTOR NG agrikultura.pptx QUARTER 4
ANG SEKTOR NG agrikultura.pptx QUARTER 4MiaBumagat1
 
Difference Between Search & Browse Methods in Odoo 17
Difference Between Search & Browse Methods in Odoo 17Difference Between Search & Browse Methods in Odoo 17
Difference Between Search & Browse Methods in Odoo 17Celine George
 
Transaction Management in Database Management System
Transaction Management in Database Management SystemTransaction Management in Database Management System
Transaction Management in Database Management SystemChristalin Nelson
 
Choosing the Right CBSE School A Comprehensive Guide for Parents
Choosing the Right CBSE School A Comprehensive Guide for ParentsChoosing the Right CBSE School A Comprehensive Guide for Parents
Choosing the Right CBSE School A Comprehensive Guide for Parentsnavabharathschool99
 
AUDIENCE THEORY -CULTIVATION THEORY - GERBNER.pptx
AUDIENCE THEORY -CULTIVATION THEORY -  GERBNER.pptxAUDIENCE THEORY -CULTIVATION THEORY -  GERBNER.pptx
AUDIENCE THEORY -CULTIVATION THEORY - GERBNER.pptxiammrhaywood
 
How to Add Barcode on PDF Report in Odoo 17
How to Add Barcode on PDF Report in Odoo 17How to Add Barcode on PDF Report in Odoo 17
How to Add Barcode on PDF Report in Odoo 17Celine George
 
Daily Lesson Plan in Mathematics Quarter 4
Daily Lesson Plan in Mathematics Quarter 4Daily Lesson Plan in Mathematics Quarter 4
Daily Lesson Plan in Mathematics Quarter 4JOYLYNSAMANIEGO
 
Music 9 - 4th quarter - Vocal Music of the Romantic Period.pptx
Music 9 - 4th quarter - Vocal Music of the Romantic Period.pptxMusic 9 - 4th quarter - Vocal Music of the Romantic Period.pptx
Music 9 - 4th quarter - Vocal Music of the Romantic Period.pptxleah joy valeriano
 

Kürzlich hochgeladen (20)

How to do quick user assign in kanban in Odoo 17 ERP
How to do quick user assign in kanban in Odoo 17 ERPHow to do quick user assign in kanban in Odoo 17 ERP
How to do quick user assign in kanban in Odoo 17 ERP
 
INTRODUCTION TO CATHOLIC CHRISTOLOGY.pptx
INTRODUCTION TO CATHOLIC CHRISTOLOGY.pptxINTRODUCTION TO CATHOLIC CHRISTOLOGY.pptx
INTRODUCTION TO CATHOLIC CHRISTOLOGY.pptx
 
Food processing presentation for bsc agriculture hons
Food processing presentation for bsc agriculture honsFood processing presentation for bsc agriculture hons
Food processing presentation for bsc agriculture hons
 
Earth Day Presentation wow hello nice great
Earth Day Presentation wow hello nice greatEarth Day Presentation wow hello nice great
Earth Day Presentation wow hello nice great
 
YOUVE_GOT_EMAIL_PRELIMS_EL_DORADO_2024.pptx
YOUVE_GOT_EMAIL_PRELIMS_EL_DORADO_2024.pptxYOUVE_GOT_EMAIL_PRELIMS_EL_DORADO_2024.pptx
YOUVE_GOT_EMAIL_PRELIMS_EL_DORADO_2024.pptx
 
Concurrency Control in Database Management system
Concurrency Control in Database Management systemConcurrency Control in Database Management system
Concurrency Control in Database Management system
 
4.16.24 Poverty and Precarity--Desmond.pptx
4.16.24 Poverty and Precarity--Desmond.pptx4.16.24 Poverty and Precarity--Desmond.pptx
4.16.24 Poverty and Precarity--Desmond.pptx
 
ICS2208 Lecture6 Notes for SL spaces.pdf
ICS2208 Lecture6 Notes for SL spaces.pdfICS2208 Lecture6 Notes for SL spaces.pdf
ICS2208 Lecture6 Notes for SL spaces.pdf
 
ROLES IN A STAGE PRODUCTION in arts.pptx
ROLES IN A STAGE PRODUCTION in arts.pptxROLES IN A STAGE PRODUCTION in arts.pptx
ROLES IN A STAGE PRODUCTION in arts.pptx
 
4.18.24 Movement Legacies, Reflection, and Review.pptx
4.18.24 Movement Legacies, Reflection, and Review.pptx4.18.24 Movement Legacies, Reflection, and Review.pptx
4.18.24 Movement Legacies, Reflection, and Review.pptx
 
Keynote by Prof. Wurzer at Nordex about IP-design
Keynote by Prof. Wurzer at Nordex about IP-designKeynote by Prof. Wurzer at Nordex about IP-design
Keynote by Prof. Wurzer at Nordex about IP-design
 
MULTIDISCIPLINRY NATURE OF THE ENVIRONMENTAL STUDIES.pptx
MULTIDISCIPLINRY NATURE OF THE ENVIRONMENTAL STUDIES.pptxMULTIDISCIPLINRY NATURE OF THE ENVIRONMENTAL STUDIES.pptx
MULTIDISCIPLINRY NATURE OF THE ENVIRONMENTAL STUDIES.pptx
 
ANG SEKTOR NG agrikultura.pptx QUARTER 4
ANG SEKTOR NG agrikultura.pptx QUARTER 4ANG SEKTOR NG agrikultura.pptx QUARTER 4
ANG SEKTOR NG agrikultura.pptx QUARTER 4
 
Difference Between Search & Browse Methods in Odoo 17
Difference Between Search & Browse Methods in Odoo 17Difference Between Search & Browse Methods in Odoo 17
Difference Between Search & Browse Methods in Odoo 17
 
Transaction Management in Database Management System
Transaction Management in Database Management SystemTransaction Management in Database Management System
Transaction Management in Database Management System
 
Choosing the Right CBSE School A Comprehensive Guide for Parents
Choosing the Right CBSE School A Comprehensive Guide for ParentsChoosing the Right CBSE School A Comprehensive Guide for Parents
Choosing the Right CBSE School A Comprehensive Guide for Parents
 
AUDIENCE THEORY -CULTIVATION THEORY - GERBNER.pptx
AUDIENCE THEORY -CULTIVATION THEORY -  GERBNER.pptxAUDIENCE THEORY -CULTIVATION THEORY -  GERBNER.pptx
AUDIENCE THEORY -CULTIVATION THEORY - GERBNER.pptx
 
How to Add Barcode on PDF Report in Odoo 17
How to Add Barcode on PDF Report in Odoo 17How to Add Barcode on PDF Report in Odoo 17
How to Add Barcode on PDF Report in Odoo 17
 
Daily Lesson Plan in Mathematics Quarter 4
Daily Lesson Plan in Mathematics Quarter 4Daily Lesson Plan in Mathematics Quarter 4
Daily Lesson Plan in Mathematics Quarter 4
 
Music 9 - 4th quarter - Vocal Music of the Romantic Period.pptx
Music 9 - 4th quarter - Vocal Music of the Romantic Period.pptxMusic 9 - 4th quarter - Vocal Music of the Romantic Period.pptx
Music 9 - 4th quarter - Vocal Music of the Romantic Period.pptx
 

asipov_vs_kaplan2_.pdf

  • 1. Leonid Asipov vs "Expression of cyanobacterial ictB in higher plants enhanced photosynthesis and growth" Leonid Asipov, 2014 Summary The aim of the original study(1) was to compare the photosynthetic properties of wt plants relative to plants transgenic with ictB, protein which is considered to be involved in carbon transport in cyanobacteria(2). The author of the original article have presented only partial data and exclusively data which was convenient for his conclusions. The full data suggests no practical advantage for an implementation of the transgenic protein in agriculture, since wt plants performed better at all the "close to reality" CO2 concentration points(300-800 PPM). For the sake of scientific truth, I will present the full data results and show how the author of the original article has been misleading in his conclusions by presenting only partial data. The original results : a single transgenic plant was presented Fig 1: The original chart containing one transgenic plant vs an average of 7 wt plants. Note that even the Y standard deviation of the wt plants was omitted. The author has presented a single transgenic plant relative to average of 7 wt plants (Fig 1). Many more transgenic plants were sampled and some of them were similar to wt, some few were better and some were worse photosynthetically (Fig 2).
  • 2. Fig 2: Presentation of all the data. Ci vs Photosynthesis curve. Dark green represents wt plants. Fig 3 : Average series of wt and transgenic plants. Ci vs Photosynthesis curve. Transgenic N=31 wt N=7. The wt plants are better at all points except the first three (50, 100 and 200 PPM). At 200 PPM, there are no visible differences between wt and the transgenic plants. In the following chart the photosynthetic properties of transgenic and wt plants has been statistically compared. The T test was calculated for each [CO2] point separately.
  • 3. Fig 4: Statistical T test of photosynthesis values at every [CO2] point separately. In the first two points (50 and 100 PPM) the differences between wt and transgenic plants are significant but meaningless since the photosynthesis value is very close to or less than 0. The CO2 concentration on the first two points (50 and 100 PPM [CO2]) is insufficient for plants growth(3) and therefore, the differences in the photosynthesis rate of plants are not practical. At 300, 400, 600 and 800 PPM [CO2], wt plants photosynthesise significantly better than the transgenic plants. Fig 5 : Citations from the original article(1). The author of the original article(1) claims that the transgenic plants photosynthesise better "under limiting but not under saturating CO2 concentrations" and therefore the protein “can be used as crop yield stimulator". To be precise, the only [CO2] point with positive photosynthesis at which the transgenic plants are significantly better than wt is 100 PPM [CO2]. The average photosynthesis
  • 4. rate of the transgenic plants at this point is 0.35 umol CO2 m-2 s-1, value which is too low for healthy plant growth. At normal earth atmosphere CO2 concentration (around 400 PPM), wt plants photosynthesise significantly better(Fig 4) and therefore there is no practical use of the transgenic protein as "crop yield stimulator". The plants growth experiments Fig 6: RH of the chamber during the experiment. The RH is “low humidity” according to the original article(Fig 7). The authors' results suggest that at lower humidity, transgenic plants grow better than wt. Such results are inconsistent with the gas exchange results, which report significantly better wt photosynthesis at 400 PPM [CO2] (Fig 8), concentration at which all the plants were grown. Since, the photosynthesis of wt plants is higher, the growth is expected to be as well. Fig 7: Citation from the original article (1). “Low humidity” is considered to be less than 30%. All the experiments were conducted at RH of less than 22% (Fig 6), value which is considered, according to the article, as "low humidity" (1). According to the presented results, at such humidity the transgenic plants grow better. Such declaration is not observed in the gas-exchange results (Fig 2).
  • 5. II Generation of plants This part of the experiment was not published or mentioned in the original article. The best transgenic plants were grown for another generation and sampled similarly to generation I. Fig 7 : Ci vs Photosynthesis generation II. All the plants. To compare the photosynthesis values only, an External [CO2] vs photosynthesis chart was built. Fig 8: External [CO2] vs Photosynthesis curve of II generation. Average series. Transgenic N=21 wt N=9.
  • 6. The results suggest that there are no significant differences between the transgenic plants and wt. Since the best transgenic plants did not pass their good properties to their progeny, I suggest that their better properties at generation I were observed due to measurement inaccuracies and not due to better genetics. Results of both generations suggest that wt plants photosynthesise equally to or better than the transgenic at all points except 50 and 100 PPM [CO2]. Values which are too low for normal plant growth. Conclusions The author of the original article has hidden data and presented false conclusions. The wt plants photosynthesise better at the normal atmosphere [CO2] concentration (Fig 4 and Fig 7). This is the reason why the transgenic protein is not practical for implementation in agriculture. Materials and Methods All measurements were made on LICOR LI6400 Portable Photosynthesis System. The illumination levels were 500 uE. All the plants were well watered before measurement. All the raw data of this article (LI6400 files) can be downloaded at : http://www.ldata.co.il/resources/proteingasexchange2.zip Citations (1) Expression of cyanobacterial ictB in higher plants enhanced photosynthesis and growth. Judy Lieman-Hurwitz, Leonid Asipov, Shimon Rachmilevitch, Yehouda Marcus, Aaron Kaplan. Plant Responses to Air Pollution and Global Change, 2005, pp 133-139 (2) A putative HCO3- transporter in the cyanobacterium Synechococcus sp. strain PCC 7942. D J Bonfil; M Ronen-Tarazi; D Sültemeyer; J Lieman-Hurwitz; D Schatz; A Kaplan FEBS letters, volume 430. Jul 2008. (3) Plant responses to low [CO2] of the past. Laci M. Gerhart and Joy K. Ward New Phytologist, volume 188 Issue 3, Sep 2010.