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Imhof otb heidelberg
1. Autologous Reimplantation
of Whole Ovine Ovaries
Martin Imhof
Department of Biomedical Sciences
Medical University of Vienna
Landesklinikum Weinviertel
Korneuburg
martin.imhof@meduniwien.ac.at
5. Experiment 1 - Whole Porcine Ovary
J Assist Reprod Genet. 2004 Dec;21(12):459-65. Cryopreservation of a whole ovary as a strategy for restoring
ovarian function. Imhof et al.
controlled fresh plunged
6. Experiment 1 - Whole Porcine Ovary
J Assist Reprod Genet. 2004 Dec;21(12):459-65. Cryopreservation of a whole ovary as a strategy for restoring
ovarian function. Imhof et al.
1,5 M DMSO
10% Albumin
RPMI - Medium
7. Freezing Curve
2°C/min to 0°C
0°C for 12 min.
1.6°C/min to -9°C
8 min at -9°C
by 0.4°C/min to
-40°C
to -150°C at the
faster rate of
10°C/min.
8. controlled frozen
Experiment 1 - Whole Porcine Ovary
J Assist Reprod Genet. 2004 Dec;21(12):459-65. Cryopreservation of a whole ovary as a strategy for restoring
ovarian function. Imhof et al.
9. plunged
Experiment 1 - Whole Porcine Ovary
J Assist Reprod Genet. 2004 Dec;21(12):459-65. Cryopreservation of a whole ovary as a strategy for restoring
ovarian function. Imhof et al.
10. Mitochondria
Endoplasm. Reticula
Experiment 1 - Whole Porcine Ovary
J Assist Reprod Genet. 2004 Dec;21(12):459-65. Cryopreservation of a whole ovary as a strategy for restoring
ovarian function. Imhof et al.
12. Exp. 2 - Whole ovine ovary
Fertil Steril. 2006 Apr;85 Suppl 1:1208-15. Orthotopic microvascular reanastomosis of whole
cryopreserved ovine ovaries resulting in pregnancy and live birth. Imhof et al.
13. Cryopreservation of a Whole Ovary
- Reimplantation of Sheep Ovaries -
step I: - unilateral ovarectomy
- cryopreservation of the ovary
step II: - contralateral explantation
and
- contralateral orthotopic
reimplantation with
microanastomosis of the ovarian vessels
- FSH-, progesterone and ultrasonic follicle screening
21. J Assist Reprod Genet. 2004 Dec;21(12):459-65. Cryopreservation of a whole ovary as a strategy for restoring
ovarian function. Imhof et al.
1,5 M DMSO
10% Autologous Serum
RPMI - Medium
Reimplantation of a Whole Ovary
- Cryosolution -
22. Slow Freezing
2°C/min to 0°C
0°C for 12 min.
1.6°C/min to -9°C
8 min at -9°C
by 0.4°C/min to
-40°C
to -150°C at the
faster rate of
10°C/min.
23. Reimplantation of a Whole Ovary
- Anastomosis -
operation
microscope
performing
vascular
anastomosis
36. Cryopreservation of Ovarian Slices
advantage: - simplified freezing technique
- easy ex- and implantation
- orthotopic position possible
- implantation repeatable
disadvantage: - absolute and relative reduced
number of follicles
42. 8 reimplantations of 4 slices
2 pregnancy´s
3 hormonale activity
2 no hormonale activity
1 primary atrophy of the ovary
Cryopreservation of Ovarian Slices
43. Conclusion
Whole ovary: - technical possible
- major operative procedure
- endothelial cells of capillary system
- not ready for application in human
Slices: - easy surgical procedure
- minor risk
- repeatable
- relative and absolute reduced
follicle pool