AMiCI (CA15114). Workshop for Early Career Investigators (ECIs) and Short Term Scientific Missions (STSM) Riga, Latvia, 7th of March 2019

1. PRESENTING THE POLY-PREVENTT PROJECT:
Developing a multi-functionalized endotracheal tube to fight polymicrobial
biofilms in a novel artificial ventilator-associated pneumonia model
Centre of Biological Engineering, LIBRO – Laboratory of Research in Biofilms Rosário Oliveira
University of Minho, Campus de Gualtar, 4700-057 Braga, Portugal Lopes, Susana P.
ACTIVITIES
VENTILATOR-ASSOCIATED PNEUMONIA (VAP)
PROJECT GOAL
Acknowledgements
o Second most common nosocomial infection
o Europe: 4.1M cases reported per year
o 50% associated to indwealling medical devices
o Leading infection in mechanically ventilated patients (10-25%)
o Highest VAP risk early following endotracheal intubation (48-72 h)
TO TAYLOR AN ANTIMICROBIAL COATING STRATEGY TO BE FURTHER
APPLIED IN THE DESIGN OF THE ETT
o The major VAP risk factor
o Often contaminated by nasopharynx/oropharynx/tracheal secretions
o Proliferation of polymicrobial biofilms along the ETT (spontaneous
biofilm break off)
• The support, through the Programa Operacional Competitividade e Internacionalização (COMPETE2020) and by national funds,
through the Portuguese Foundation for Science and Technology (FCT), of the POLY-PrevEnTT project (PTDC/BTM-SAL/29841/2017-
POCI-01-0145-FEDER-029841);
• The support of FCT, under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-
0145-FEDER-000004) funded by the European Regional Development Fund (FEDER) under the scope of Norte2020 - Programa
Operacional Regional do Norte;
• The support of FCT, FEDER, Portugal2020 and COMPETE202 to the Coded-FISH project (PTDC/DTP-PIC/4562/2014);
• The POLY-PrevEnTT team also ackoowledge AMiCI Meeting for the invitation
ANTIMICROBIAL-COATED ETT
ANTI-ADHESIVE BIOCOMPATIBLEANTI-BIOFILM
RISK FOR VAP PREDISPOSITION
ANTIMICROBIAL RESISTANCE
ANTIMICROBIAL COATING
FORMULATIONS:
o IN VITRO SCREENING OF
PROMISING AGENTS
o IMMOBILIZATION ONTO
PVC SURFACES TOWARDS
SINGLE SPECIES
CLASI-FISH DEVELOPMENT:
o IN SILICO DESIGN/
THEORETICAL
PARAMETERS
o EXPERIMENTAL
VALIDATION IN VAP
POLYMICROBIAL
BIOFILMS
MORTALITY/MORBIDITY RATES
SOCIOECONOMIC COSTS
CO-IMMOBILIZATION
STUDIES:
o CO-IMMOBILIZATION
ONTO PVC SURFACES
o IN VITRO PERFORMANCE
AGAINST
POLYMICROBIAL
BIOFILMS
FEASIBILITY OF THE
MODIFIED PVC SURFACE
WITH THE BEST TRAITS:
o ASSESSMENT TOWARDS
EXPANDED VAP
COMMUNITIES
o USE OF VAP IN VIVO-LIKE
CONDITIONS
VALIDATION OF THE NOVEL ANTIMICROBIAL-
COATED ETT:
o DEVELOPMENT/ADAPTATION OF AN
ARTIFICIAL DYNAMIC MODEL SIMULATING
AN ORO-INTUBATED PATIENT
Figure 1.
Proposed dynamic VAP model.
SUSANA
LOPES
MARIA O.
PEREIRA
DIANA
ALVES
PAULA
JORGE
ANDREIA
MAGALHÃES
ANA M.
SOUSA
NUNO
AZEVEDO
NUNO
GUIMARÃES
RICARDO
FERNANDES
TOTAL UMinho UPorto
225.133,13 € 189.508,13 € 36.625,00 €
PERSISTENT LOWER AIRWAY INFECTION
(B)
#
pDA+antifungal agent immobilization – Candida albicans CLASI-FISH - development and validation
Cytotoxicity (MTS) Cell cultivability
Fluorochromes:
• Alexa Fluor 405 (hot yellow)
• Alexa Fluor 488 (red)
• Alexa Fluor 514 (green)
• ATTO 532 (blue)
• ATTO 550 (cyan)
• Alexa Fluor 594 (magenta)
• ATTO 633 (yellow)
• ATTO 655 (grey)
1 h (cells) + 23 h (artificial urine): 1x108 CFU/mL
Bacterial mixture
Hybridization with
EUB LNA/2`OMe
probe coupled to 8
fluorochromes
ENDOTRACHEAL TUBE (ETT)
SOME PRELIMINARY RESULTS
TEAMBUDGET
pDA pDA +
antifungal
PDMS pDA pDA +
antifungal
PDMS
pDA
pDA + antifungal
To
Ventilator
ETT cuff inflated
Plastic syringe
(trachea)
Broth media
Cellviability/%
Log10CFU/mL
https://www.ceb.uminho.pt/Projects/Details/6147
PTDC/BTM-SAL/29841/2017-POCI-01-0145-FEDER-029841