1.
Centrifugation
Made by KASHISH WILSON
ASSISTANT PROFESSOR
MM COLLEGE OF PHARMACY,MM(DU) MULLANA

2.
Introduction
 Centrifugation is a method of separating molecules having different densities
by spinning them in solution around an axis (in a centrifuge rotor) at high
speed. It is one of the most useful and frequently employed techniques in the
molecular biology laboratory.

3.
Objective & Principle
 Objective.
 To purify the component by removing impurities in the supernatant liquid. 3)
To separate crystalline drugs from the mother liquor. 4) To test the emulsion
and suspensions for creaming and sedimentation at an accelerated speed.
 Principle.
 Centrifuge works by using the principle of sedimentation: Under the influence
of gravitational force (g-force), substances separate according to their
density. Different types of separation are known, including isopycnic,
ultrafiltration, density gradient, phase separation, and pelleting.

4.
Application..
 The extraction of fat from milk in order to produce skimmed milk.
 The removal of water from moist lettuce with the help of a salad spinner.
 The Spin-drying of water in washing machines in order to remove water from
the clothing.
 The separation of solid blood and urine materials into forensic and testing
laboratories.
 Centrifugation is used to collect cells, to precipitate DNA, to purify virus
particles, and to distinguish subtle differences in the conformation of
molecules. Most laboratories undertaking active research will have more than
one type of centrifuge, each capable of using a variety of rotors.

5.
Thanks....