Asian Federation of Biotechology Regional Symposium 2017

9th
Asian Federation of Biotechnology (AFOB)
Regional Symposium 2017 (ARS 2017)
New Innovations and Emerging Technologies in Asian Biotechnology
February 9-11, 2017 at De La Salle University, Manila, Philippines
DOST - PCIEERD
9
TH
Asian Federation of Biotechnology
REGIONAL SYMPOSIUM
in conjunction with
DOST East Asia Joint Research Program
4
th
National Workshop 2017
“New Innovations and Emerging Technologies in Asian
Biotechnology”
De La Salle University
Manila Philippines
February 9-11, 2017
BOOK OF ABSTRACT
DOST - PCIEERD
scCO2
Biochemical
& Bioprocess
Agricultural
Biotechnology
Applied
Microbiology
Biopharmaceutical
& Medical
Biotechnology
Biocatalysis
Environmental
Biotechnology Renewable
Bioenergy
ISSN No. 2545-9643
0

9th
DOST - PCIEERD
9TH
Asian Federation of Biotechnology
REGIONAL SYMPOSIUM
in conjunction with
DOST East Asia Joint Research Program
4
th
National Workshop 2017
“New Innovations and Emerging Technologies
in Asian Biotechnology’’
February 9 - 11, 2017
De La Salle University, Manila, Philippines
ISSN No. 2545-9643
DOST-PCIEERD 0

9th
DOST - PCIEERD
The 9th
AFOB Regional Symposium (ARS 2017) is an annual event of Asian
Federation of Biotechnology (AFOB). This time, the ARS 2017 will be hosted by the Chemical
Engineering Department, Gokongwei College of Engineering, De La Salle University in coordination
with DOST-PCIEERD and Asian Federation of Biotechnology from February 09-11, 2017.
Objectives of the Symposium includes:
1. To provide a venue where best practices in Biotechnology are shared;
2. To serve as springboard for new areas of research in Biotechnology;
3. To sustain the strong partnership and linkages among AFOB members and collaboration with non-
member institutions, industry and government.
4. To present the advanced research results among scientists of universities, institutes, various
companies in the Philippines and other countries under AFOB’s partnership.
Scope of the Symposium
Scope covers all areas of research within the emerging technologies in Biochemical,
Biotechnology, Biosciences and Biological Engineering. Topics cover the fields of Biochemical
Engineering, Biotechnology, Biosciences and Chemical Engineering topics related to Bioprocesses
and Bioreactor Engineering. Contributions for oral and poster presentations in the following area
 Biochemical and Bioprocess Engineering Materials and Nanotechnology
 Agricultural Biotechnology
 Applied Microbiology
 Biopharmaceutical and Medical Biotechnology
 Biocatalysis and Protein Engineering
 Environmental Biotechnology
 Renewable Bioenergy
The Organizing Committee
0

9th
DOST - PCIEERD
Table of Contents
ARS 2017 Scope
Messages
List of Presentations
Plenary
Lectures
Title / Plenary Speaker
PL1 Emerging Technologies and New Innovations in Biotechnology, A Boom or
a Bane? / Jose Santos Carandang . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
PL2 Bioreactive Separation with CO2-H2O Hybrid Synergy / Armando Quitain,
Akito Hashimoto, Saya Yamafuku, Mitsuru Sasaki, and Tetsuya Kida . . . . . . . . . . 3
PL3 Catalytic and Electrochemical Applications of Graphene Oxide in
Biomass Conversion / Tetsuya Kida and Armando Quitain . . . . . . . . . . . . . . 4
PL4 Sub and Supercritical Fluid Technology as Platform Technology for
Biochemicals and Biofuels Processing / Artiwan Shotipruk . . . . . . . . . . . . . . . 5
PL5 Biofouling Control in Membrane Filtration System by Quorom Quenching
Bacteria / Yinhua Wan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
PL6 Development of Enzymatic Fuel Cell System / Dong Sup Kim, Ju Hun Lee,
Soo Kwon Lee, Ji Hyun Yang1
, and Seung Wook Kim . . . . . . . . . . . . . . . . . . . . . 7
PL7 Innovative Technology of Biomass Conversion into Value-added
Bioproducts / Suraini Abd-aziz, Mohd Azwan Jenol, Ruqayyah Masran, Nahrul
Hayawin Zainal, and Mohamad Faizal Ibrahim . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Oral
Presentations
Title / Authors
A1 Effects of Light Conditions on the In vitro Growth of Microalgae
Nannochloropsis Oculata / Cam Tu Trinh, Thanh Huong Tran, and Trang Viet
Buii . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
A2 Factors Affecting Bioconversion of Reutealis Trisperma Seed by Black
Soldier Fly Larvae for the Production of Protein and Oil-rich Biomass /
Muhammad Yusuf Abduh, Mochamad Hirza Nadia, Syaripudin, and Robert
Manurung . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
A3 Elucidation of Thiamine (Vitamin B1) Biosynthesis Pathway in Gracilaria
sp. and the Effect of Stress Towards Thiamine Biosynthesis / Nur Sakinah
Meor Mohd Ghazali and Zetty Norhana Balia Yusof . . . . . . . . . . . . . . . . . . . . . 12
A4 Bioconversion of Rubber Seed to Produce Protein and Oil-rich Biomass
Employing Black Soldier Fly Larvae Assisted by Microbes / Muhammad
Yusuf Abduh, Maryam Jamilah, Pramesti Istiandari, Mochamad Hirza Nadia,
Syaripudin, and Robert Manurung . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
0

9th
DOST - PCIEERD
A5 Comparison of Yield of Reducing Sugar Obtained from Hydrothermal and
Alkali Pretreated Brown Seaweed “Sargassum sp.” / Kyaw Wunna, Joseph
Auresenia, Leonila Abella, Pag-asa gaspillo, Kiyohiko Nakasaki. . . . . . . . . . . . 14
A6 A Review on Abaca Fiber Reinforced Composites / Joefreim Delicano . . . . . . 15
A7 Microalgal Lipid Extraction by Supercritical Carbon Dioxide and its
Conversion to Fatty Acid Methyl Ester / Alva Durian, Maria Cristina
Macawile, Jurex Gallo, and Joseph Auresenia . . . . . . . . . . . . . . . . . . . . . . . . . . 16
A8 Extraction of Kenaf Seed Oil and Kakawate Seed Oil Using Supercritical
Carbon Dioxide / Maria Cristina Macawile, Alva Durian, Jurex Gallo, Luis
Razon, Raymond Girard Tan, and Joseph Auresenia . . . . . . . . . . . . . . . . . . . . . 17
A9 Transdermal Cancer Vaccine by Solid-in-Oil(S/O) Nanodispersions /
Masahiro Goto, Masato Sakuragi, Rie Wakabayashi, and Noriho Kamiya . . . . 18
A10 Design of Novel Biocatalysts by Enzymatic Biomolecular Conjugation /
Noriho Kamiya, Mari Takahara, Rie Wakabayashi, and Masahiro Goto . . . . . 19
A11 Microwave-assisted Graphene Oxide Catalysis for Cascade Synthesis of
Advanced Biofuel from Waste Oil / Yoshifumi Sumigawa, Armando Quitain,
Mitsuru Sasaki, and Tetsuya Kida . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
B1 Determination of Antifungal Activities from Malaysian Seaweed Extracts
Against Ganoderma Boninense, A Disease-Causing Fungus in Oil Palm
(Elaies Guineensis) / Syamimi Diyana Binti Abdul Aziz and Zetty Norhana
Balia Yusof . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
B2 Effect of Different Extraction Conditions on the Yield of Pectin Extracted
from Bilimbi (Averrhoa Bilimbi) / Reygan Sangalang . . . . . . . . . . . . . . . . . . . 22
B3 Preliminary Study of the Production of Phycobiliproteins from Gracilaria
changii / Rugi Vicente Rubi, Erison Roque, Fritzzie Gutierrez, May Anne
Hegin, Jeanell Martin, Arrianne Jaye Mata, and Ervin Mission . . . . . . . . . . . . 23
B4 An Unique Nicotine-Degrading Bacterium Revealed by Multi-Hierarchy
Analysis / Jun Li, Lie Xiong, Yang Jiao, Houlong He, and Weihong Zhong . . . 24
B5 Selectivity Cytotoxicity of Bioluminescent Bacteria Isolated from Fresh Ink
of Philippine Squid on Human Colon Cancer and Normal Cell Lines /
Jocelyn Luyon, Glenn Oyong, and Esperanza Cabrera . . . . . . . . . . . . . . . . . . . . 25
B6 Effect of Monoethylamine Concentration on Biomass Growth, CO2
Fixation and Fatty Acid Profile of Chlorella sp and Thalasiorisa sp. Using
Two (2) Operations in a Flask Flat Plate Photobioreactor / Racquel Ranchie
Ting, Jose Simon Simangan, Alyzza Marie Narciso, and Joseph Auresenia . . . . 26
B7 Enzymatic Saccharification Enhancement by Morphological and
Structural Modification of Rice Husk Subjected to High Pressure Steam
Pretreatment / Muinat Olanike Kazeem, Umi Kalsom Md Shah, Azhari Samsu
Baharuddin, and Nor’ Aini Abdul Rahman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

9th
DOST - PCIEERD
B8 Characterization of Metabolic Pathways in Marine Microalgae through In
Silico Model / Seong-Joo Hong, Byung-Kwan Cho, Dong-Myung Kim,
Hookeun Lee, Hyung-Kyoon Choi, and Choul-Gyun Lee . . . . . . . . . . . . . . . . . . 28
B9 Formulation and In Vitro Evaluation of Surface-Coated Nanocarrier for
Etoposide / Czarina Dominique Rodriguez, Rie Wakabayashi, Noriho Kamiya,
and Masahiro Goto . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
B10 Transcutaneous Immunotherapy for Pollinosis Using Solid-in-Oil
Nanodispersion / Qingliang Kong, Momoko Kitaoka, Rie Wakabayashi,
Noriho Kamiya, and Masahiro Goto . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
B11 Rubber Seed Oil Extraction for Biodiesel Production by Liquified
Dimethyl Ether / Panatpong Boonnoun, Pamornrat Chantam, Watanachai
Yaowarat, and Artiwan Shotipruk . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
C1 Synthesis of CNT for PET Bottles, Sargassum using Simultaneous
Microwave Assisted Pyrolysis and Microwave Plasma – Chemical Vapor
Deposition / Jan Patrick Si, Mark Angelo Tionson, Francis Ian Tobias, and
Joseph Auresenia . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2 Performance Optimization of DSSC by Varying Sensitized Dye Ratios
Metal Dopant in Photodiode Sensitizer, Dye Acidity and Type Of Stability
Co-Pigment / Maria Eliza Cleofas, Ginelle Galido, Eulene Geronimo, and
C3 Optimization of DSSC with Metal Doped TiO2 Photoanode and Various
Electrode / Kendrick Chiu, Tiffany Joy Ong Hian Huy, Franco Sosuan, and
C4 Isolation and Identification of Chlorella-like Species for Potential
Application in Biofuel Production / Nur Faiqah Ismail, Mardiana Mohd
Ashaari, Shaharah Mohd Idris, Normawaty Mohammad Noor, and Khairul
Bariyyah Abd Halim . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
C5 Identification and Structural Analysis of Thiamine Pyrophosphate (TPP)
Riboswitch in Oil Palm (Elaeis Guineensis) / Atiqah Subki and Zetty Norhana
Balia Yusof . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
C6 Clicking the Switch of Microbial Secondary Metabolism: Signal
Transduction Engineering / Jian-Jiang Zhong . . . . . . . . . . . . . . . . . . . . . . . . . 37
C7 Varying Proportions of Lactic Acid Bacteria (LAB) Serum and Molasses
for Production of Lactic Acid Bacteria from Rice Washings / Baby Ariane
Caraig, Ed Rol John Manzanilla, and Amor Magtibay . . . . . . . . . . . . . . . . . . . . 38
C8 Upland Surface Water and Genotoxicity Assessment of Watershed Areas
of Cavite, Luzon Island, Philippines / J.P. Macawile, Marlon Pareja, J.P..
Luyon, and A.E Castro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
C9 Removal of Glycerides from Rice Bran Acid Oil by Hydrolysis with
Subcritical Water / Amornrat Meedam and Artiwan Shotipruk . . . . . . . . . . . . . 40

9th
DOST - PCIEERD
Poster
Presentations
Title / Authors
P1 Biosynthesis of Indigo Dye from Glucose Using CYP102A_SCAT
Monooxygenase / Hee-Jung Kim, Seyoung Jang, and Kwon-Young Choi . . . . . 42
P2 Bacterial Expansin as Novel Subunit in Agarolytic Complexes for Enhanced
Degradation of Red Seaweeds by Loosening Structure / Da Woon Jeong,
Young-Chul Joo, Jeong Eun Hyeon, Sang Kyu Shin, And Sung Ok Han . . . . . . . . . . . 43
P3 SAM Preparation from Mutated Yeast / Wen-Feng Sung and Jiumn-Yih Wu 44
P4 Production of Human Milk Oligosaccharides in Engineered Escherichia
coli / Young-Wook Chin and Jin-Ho Seo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
P5 Bioethanol Production by Co-culture of Trichoderma reesei and Yeasts
from Cellulose / Jianning Zheng and Beom Soo Kim . . . . . . . . . . . . . . . . . . . . . 46
P6 Effect of Electromagnetic Field on Melanogenesis with B16f10 Melanoma /
Yu-Mi Kim, Young-Kwon Seo, and Jung-Keug Park. . . . . . . . . . . . . . . . . . . . . . 47
P7 A Simple Route to Prepare Gold Nanoparticle/ Polyethyleneimine/ reduced
Graphene Oxide Composite and its Application to NADH Detection / Sheng
Chuo and Jau-Yann Wu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
P8 Preparation of Glutathione (GSH)-Bound Magnetic Nanoparticles for
Enzyme Immobilization and Application / Jian-Wen Lai, Wei-Chih Kuan,
and Wen-Chien Lee . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
P9 Biotransformation of Oleic Acid into 9-(Nonanoyloxy) Nonaoic Acid using
Baeyer-Villiger Monooxygenase from Dietzia Sp D5. / Gaun Lee, Joo-Hyung
Yun, Pamidimarri d. V. N. Sudheer, and Kwon-Young Choi . . . . . . . . . . . . . . . . 50
P10 Ionic Liquid Mediated Caffeic Acid Ester Synthesis using Mycelia of
Halotolerant Aspergillus Niger as a Whole-Cell Biocatalyst / Govindaraju
Rajapriya, Vivek Kumar Morya, and Yoon-Mo Koo . . . . . . . . . . . . . . . . . . . . . . 51
P11 Hairpin Probe-based Isothermal Amplification Technology for Target
Nucleic Acid Detection / Hyo Yong Kim, Jun Ki Ahn, and Hyun Gyu Park . . . 52
P12 Development of Hydrothermal Liquefaction Process using Microalgae for
Improvement of Biocrude as Well as Phosphorous Contents / Cheol-Min
Yang and Jong-In Won . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
P13 Effect of Nitrogen Starvation on Lipid and Carbohydrate Accumulation in
Tetraselmis Species / Han-Byeol Kim, Seong-Joo Hong, and Choul-Gyun Lee . 54
P14 Improving Wave Energy Utilization into Algal Culture Mixing in Floating
Pond System to Increase Biomass Productivity / Injae Jung, Jong-Chan Lee,
Hanwool Park, Sang-Min Lim, and Choul-Gyun Lee . . . . . . . . . . . . . . . . . . . . . 55
P15 Production of Polyphenolic Compounds from Lipid Extracted Microalgae
using Microwave-Assisted Extraction / Junghyun Jin, Bora Min, Jo Jaemin,
and Kim Jinwoo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
P16 Production of Carbon Nanotubes from Coconut Biomass using Microwave
Assisted Processes / John Ephraim Torres and Joseph Auresenia . . . . . . . . . . . 57

9th
DOST - PCIEERD
P17 Optimization of Uncatalyzed Direct Biodiesel Production from Wet
Microalgae under Subcritical Conditions using Definitive Screening Design
/ Charles Felix, Alchris Woo Go, Sylviana Sutanto, Phuong Lan Tran-Nguyen,
Aristotle Ubando, Cynthia Madrazo, Alvin Culaba, Yi-Hsu Ju, Jo-Shu Chang . 58
P18 Detection of Shiga-Toxin Producing Escherichia Coli in Beef Sold in
Dasmarinas City Markets / Arizaldo Castro, Thessa Marie L. Urquia, and
Norbel A. Tabo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
P19 Improvement of 2,3-Butanediol Production by Acetate Addition in
Enterobacter Aerogenes ATCC 29007 / Sang Jun Lee, Dong Sup Kim, Ju Hun
Lee, Soo Kwon Lee, Ji Hyun Yang, and Seung Wook Kim . . . . . . . . . . . . . . . . . 60
Organizing Committee
0

9th
DOST - PCIEERD
DE LA SALLE UNIVERSITY
OFFICE OF THE PRESIDENT
`
MESSAGE
Congratulations to the organizing committee of the 9th Asian Federation of Biotechnology
(AFOB) Regional Symposium (ARS 2017) for coming up with a Book of Abstracts. This book
showcases the research outputs of scientists from member countries of AFOB, and those of other
researchers engaged in biotechnology. It is an important contribution to the current wealth of
materials on Asian Biotechnology covering a broad range of topics from Biochemical, Biotechnology,
Biosciences and Biological Engineering processes and applications that can help scientists and
practitioners to validate, challenge, or reinforce existing knowledge in the field.
I wish the organizing committee all the best in this worthwhile endeavor.
BR. RAYMUNDO SUPLIDO FSC
President
0

9th
DOST - PCIEERD
OFFICE OF THE VICE CHANCELLOR FOR RESEARCH AND INNOVATION
`
MESSAGE
Scientists in the 21st
Century face challenges that are distinctly different from those that existed
more than a century ago. The rise of new industrial sectors has led to shifts in focus to non-traditional
areas such as biotechnology and nanotechnology. The internet has also made the exchange of
information so effortless, that scientists now work in a highly globalized, and increasingly borderless,
world.
Academic conferences provide valuable opportunities for the implications of such historic
milestones to be discussed among colleagues. This year, as the 9th Asian Federation of
Biotechnology (AFOB) Regional Symposium (ARS 2017) with the theme “New Innovations and
Emerging Technologies in Asian Biotechnology”, researchers in biotechnology and allied fields will
have a chance to exchange ideas that deal with current regional and global issues.
I wish to congratulate the organizing committee for organizing this important event, and wish
the delegates a vibrant, fruitful experience during the conference.
RAYMOND R. TAN, PHD
Vice Chancellor for Research and Innovation
De La Salle University 0

9th
DOST - PCIEERD
GOKONGWEI COLLEGE OF ENGINEERING
`
MESSAGE
I am pleased to welcome you to the 9th Asian Federation of Biotechnology (AFOB)
Regional Symposium (ARS 2017). With the theme, “New Innovations and Emerging Technologies
in Asian Biotechnology”, I am confident that this conference will be successful in bringing together
representatives from Asian academic institutions, industries and various research organizations with
the aim of enhancing research in various fields in Biotechnology.
I would also like to express my sincere appreciation to the Asian Federation of Biotechnology
and the Department of Science and Technology – Philippine Council for Industry, Energy and
Emerging Technology Research and Development for supporting this conference. My special thanks
are due to the Chemical Engineering Department’s DOST East Asia Research Program of De La Salle
University as the main organizer of the conference who have worked hard to ensure the success of the
event.
Thank you and wishing you all a constructive and fruitful conference.
JONATHAN DUNGCA, PHD
Dean, Gokongwei College of Engineering
0

9th
DOST - PCIEERD
GOKONGWEI COLLEGE OF ENGINEERING
CHEMICAL ENGINEERING DEPARTMENT
`
MESSAGE
It is our great pleasure and honor to welcome you to the 9th
Asian Federation of Biotechnology (AFOB) Regional Symposium
(ARS 2017) with the theme, “New Innovations and Emerging
Technologies in Asian Biotechnology”.
The Department of Chemical Engineering’s DOST East-Asia
Joint Research Program of De La Salle University (DLSU) with the Asian Federation of
Biotechnology (AFOB) and the Department of Science and Technology – Philippine Council for
Industry, Energy and Emerging Technology Research and Development (DOST-PCIEERD) are
joining hands in organizing this Asian Regional Conference. We would like to give our sincerest
appreciation and gratitude to all the participants and sponsors for supporting this event and
contributing for the success of this conference.
The AFOB Regional Conferences are organized annually to serve as a venue to disseminate
the outputs of research among the member countries in advancing biotechnology research and
development. The regional conference also involves participants from the academe, industry,
government and other research institutions and professional organizations.
On behalf of the DOST East-Asia Joint Research Program of the Chemical Engineering
Department of De La Salle University and the co-organizers, we wish that your stay in our country is
pleasant and enjoyable and may your participation to this conference be fruitful and full of learning
experiences. May this event foster future research collaborations as well as lasting friendships among
the participants from various countries and member Institutions.
Welcome and MABUHAY!
Joseph Auresenia, PhD
Chairman of the Organizing Committee
9th
Asian Federation of Biotechnology Regional Symposium
De La Salle University 0

9th
DOST - PCIEERD
Abstracts of
Plenary Lectures
0

9th
DOST - PCIEERD
EMERGING TECHNOLOGIES AND NEW INNOVATIONS IN
BIOTECHNOLOGY, A BOOM OR A BANE?
Jose Santos Carandang1
1
College of Science, De La Salle University, 2401 Taft Avenue, 1004 Manila, Philippines
Email: jose.santos.carandang@dlsu.edu.ph
Traditional biotechnology has provided us with innovations in food processing, more
productive agriculture, biodiversity conservation and improved health care. Modern biotechnology
fosters new opportunities and solutions to persistent and even pestering problems including food
security issues related to climate change. However, risks created by the innovations and product
applications should be precluded. For us to be able to maximize the benefits of globalization of
biotechnology, ethical issues related to biotech health care need to be addressed as well. The 21st
century is the century of science and in the forefront are modern biotechnological innovations. Here
in Asia, both developed and emerging economies will be driven by new innovations and emerging
technologies even those economies that were previously primarily investment driven.
Dr. Jose Santos R. Carandang VI is a full professor of Biology at De La Salle University
where he is currently the Dean of the College of Science. His recent research involvement
is on food security in collaboration with Dr. Robert Taylor of Montclair State University,
New Jersey in the USA, as well as in the management and biological control of invasive
species affecting food production. Dr. Carandang is a product of the University of
Wuerzburg in Germany where he earned his doctorate in Natural Science and where he did
a number of his post-doctoral researches. He also has a Master in National Security
Administration degree from the National Defense College of the Philippines and a Master
of Science in Biology from De La Salle University. Dr. Carandang was the founding director
of the De La Salle Food Institute. He also was the Member for Biology of the Commission
on Higher Education - Technical Panel for Science and Mathematics.
2

9th
DOST - PCIEERD
BIOREACTIVE SEPARATION WITH CO2-H2O HYBRID SYNERGY
Armando Quitain1,2,3*
, Akito Hashimoto1
, Saya Yamafuku1
, Mitsuru Sasaki4
and Tetsuya Kida1
1
Faculty of Advanced Science and Technology, 2
Graduate School of Science and Technology
3
International Research Organization for Advanced Science and Technology, 4
Institute of Pulsed Power Science
Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto, 860-8555, Japan
*E-mail: quitain@kumamoto-u.ac.jp
Hybridism, or blending two synergistic components to enhance overall process performance
and efficiency, is currently gaining global popularity. This concept also applies to any processes,
including those related to biotechnology or biochemical engineering. This talk highlights how the
hybrid synergy of two environmentally benign solvents, CO2 and H2O under pressurized conditions,
could drive more efficient hydrolysis of bioactive compounds through simultaneous reaction and
separation. The proposed system is applied to natural bioactives which normally contain carbohydrates
covalently linked with other compounds such as proteins, lipids and aglycones. Removal of the sugar
moieties by hydrolysis into more bioactive and bioavailable materials are advantageous especially to
nutraceutical and pharmaceutical sectors. Hesperidin, a citrus bioflavonoid, for example, can be
converted to hesperetin by removing the sugar components. The conventional method employs
harmful catalysts such as sulfuric acid, obtaining low yield due to formation of intermediates. In
addition, separation of the target compounds from the reaction products requires rather tedious and
complicated steps. The proposed system addresses the abovementioned drawbacks of the conventional
method by employing the hybrid synergy of subcritical H2O and supercritical CO2 to drive reactive
separation towards higher yield and more selective extraction of the target compound.
Keywords: supercritical CO2, subcritical H2O, bioflavonoids, aglycones, hydrolysis
Dr. Armando Quitain is at present an Assistant Professor of the Graduate School of
Science and Technology, Department of Applied Chemistry and Biochemistry,
Kumamoto University (Japan) and con concurrently holds a post as International
Research Collaborator at the International Research Organization for Advanced Science
and Technology. He received his MS in Chemical Engineering at De La Salle University,
Manila and PhD in Chemical Engineering as Monbusho scholar at Nagoya University,
Japan. His main research interests include application of microwave and supercritical
fluid technologies to biomass conversion into useful chemicals and fuels.
3

9th
DOST - PCIEERD
CATALYTIC AND ELECTROCHEMICAL APPLICATIONS OF
GRAPHENE OXIDE IN BIOMASS CONVERSION
Tetsuya Kida1*
and Armando Quitain1
1
Division of Materials Science, Faculty of Advanced Science and Technology,
Kumamoto University, Kumamoto 860-8555, Japan
*E-mail: tetsuya@kumamoto-u.ac.jp
A two-dimensional nanosheet of graphene oxide (GO) has attracted a great deal of attention due to
its potential versatile applications. GO contains only earth abundant, non-toxic element of carbon.
Furthermore, it can be synthesized from graphite by simple chemical processes including oxidation and
exfoliation. These advantages make GO promising alternative to rare and expensive materials that are
used for some applications. One of the novel properties of GO is its high proton conductivity, allowing
GO to be used as proton exchange membranes for fuel cells. The proton conductivities arise from proton
hopping at oxygen functional groups originally anchored to the GO surface. It has also been reported that
the proton conductivities can be tuned by changing the oxygen content in GO. The partial reduction makes
GO a mixed conducting material that shows both of protonic and electronic conductivities. Using GO’s
high proton conductivity that is comparable to Nafion, we have developed new carbon-based
electrochemical devices such as hydrogen separation devices, water electrolysis devices, and hydrogen
sensors. We are also utilizing a high acidity of GO to convert biomass into valuable compounds by
hydrolysis. The proton conductive properties of GO can generate active proton species, which would be
utilized for biomass conversion.
Keywords: graphene oxide, biomass, cellulose, biofuel, proton conductivity
Dr. Tetsuya Kida has been a Professor at Kumamoto University since 2013. He received
his M. Eng. Degree in Materials science and his Dr. Eng. Degree from Kyushu University.
He was an Associate Professor at Kyushu University from 2006 to 2013 and an Assistant
Professor at Saga University from 2003 to 2006. His current research topics are synthesis
of inorganic nanomaterials such as quantum dots, oxide nanocrystals, and grapheme oxide,
and their application for solar energy conversion, gas sensing, gas separation membranes,
etc.
4

9th
DOST - PCIEERD
SUB AND SUPERCRITICAL FLUID TECHNOLOGY AS PLATFORM
TECHNOLOGY FOR BIOCHEMICALS AND BIOFUELS PROCESSING
Artiwan Shotipruk1
*
1
Chemical Engineering Research Unit for Value Adding of Bioresources, Department of Chemical Engineering, Faculty
of Engineering, Chulalongkorn University, Phayathai Rd., Patumwan, Bangkok 10330, Thailand
*Email: Artiwan.sh@chula.ac.th
Recent research findings at the Chemical Engineering Research Unit for Value Adding of
Bioresources, Chulalongkorn University, will be summarized, especially those involves the use of sub
and supercritical fluids technology for the processing of bioactive compounds and production of fuels
and fine chemicals from biomass. For biomass conversion, not only subcritical water is used in the
production of important chemical intermediates for biofuels and biochemicals, namely 5-
hydroxymethylfurfural (HMF), the process can also be applied to develop low-cost carbon support for
an acid catalyst. The suitable synthesis conditions of this rather new so called hydrothermal carbon
were determined. The catalyst prepared by sulfonation of this carbon was evaluated for its applicability
for biomass conversion. As for the application of subcritical and supercritical fluids technology to the
production of high value active compounds, we have successfully devised various processes including
supercritical carbon dioxide (SC-CO2) extraction and supercritical anti-solvent (SAS) micronization
to produce high purity free lutein from flowers of marigold, an important ornamental plant, prevalently
grown in Thailand. The processes and key results will also be highlighted in this talk.
Prof. Artiwan Shotipruk received her M.Sc. and Ph.D. from the University of Michigan.
Her research interest is on subcritical and supercritical fluids technology, especially for
extraction of natural products and biomass conversion. She received a number of national
awards such as Young Scientist Award from the Foundation for the Promotion of Science
and Technology under Patronage of His Majesty the King, Young Researcher Award from
Thailand Research Funds and the Commission for Higher Education, For Women in Science
fellowship from UNESCO-L’Oreal Thailand, Excellent Research Output Award from
National Research Council of Thailand, and TRF-CHE-SCOPUS Young Scientist Award.
She is now the Head of Chemical Engineering Research Unit in Value Adding of
Bioresources at Chulalongkorn University, and currently serves as an assistant director of
the Royal Golden Jubilee Ph.D. Program, Thailand Research Fund.
45

9th
DOST - PCIEERD
BIOFOULING CONTROL IN MEMBRANE FILTRATION SYSTEM BY
QUOROM QUENCHING BACTERIA
Yinhua Wan1
*
1
University of Chinese Academy of Sciences, China
*Email: yhwan@ipe.ac.cn
Membrane biofouling is the Achilles heel to membrane bioreactor (MBR) for wastewater
treatment. In the present work, a newly isolated indigenous quorum quenching (QQ) Bacillus
methylotrophicus sp. WY (B. methylotrophicus) was applied to membrane filtration system, with the
aim to inhibit signaling phenomena via QQ mechanism. The effect of different beads and entrapped
QQ biomass on biofouling control behavior and as well as on the QQ activity were examined in detail.
It was confirmed that different bead size and amount of initially entrapped QQ biomass substantially
affected the quorum quenching activity. Further, it was found that QQ activity was more pronounced
in a standard N-acyl homoserine lactone (AHL) solution and membrane operation system when
decreasing the bead size and increasing the initially entrapped QQ biomass. Additionally, various
characteristics of the strain WY were evaluated in detail such as AHL-lactonase by re-lactonisation
bioassay and localization of AHL-lactonase, confirming that the strain WY has intracellular QQ
mechanism, i.e., it secrets AHL-lactonase and keeps them inside the cell.
Keyword: quorum quenching, biofouling, membrane bioreactor, AHL-lactonase, wastewater treatment
Prof. Yinhua Wan is a Professor of Biochemical Engineering, working at Institute of
Process Engineering, Chinese Academy of Sciences (CAS), Beijing, China. He obtained his
Ph.D. in Environmental Chemical Engineering from South China University of Technology,
Guangzhou, China and D. Phil. in Biochemical Engineering from Oxford University, Oxford,
UK. He is currently serving as Vice Director of National Engineering Research Centre for
Biotechnology (Beijing) and Vice Director of State Key Laboratory of Biochemical
Engineering, Institute of Process Engineering, CAS. He is also serving as Vice Secretary
General of Chinese Society of Biotechnology and Executive Board Member of Asian
Federation of Biotechnology. Currently, Prof. Wan’s research covers a wide range of
technology developments for wastewater treatment and biorefinery of lignocellulosic
materials, including industrial wastewater treatment, pretreatment of feedstocks,
optimization of fermentation process and bioseparation. So far, he has published more than
160 peer-reviewed papers, 4 co-authored monographs and filed 36 patents (26 granted,
including 2 US patents).
6

9th
DOST - PCIEERD
DEVELOPMENT OF ENZYMATIC FUEL CELL SYSTEM
Dong Sup Kim1
, Ju Hun Lee1
, Soo Kwon Lee1
, Ji Hyun Yang1
, and Seung Wook Kim1
*
1
Department of Chemical and Biological Engineering, Korea University, 145 Anam-Ro, Seongbuk-Gu, Seoul 136-701,
Republic of Korea
*E-mail: kimsw@korea.ac.kr
Enzymatic fuel cell (EFC) system has been proposed that chemical energy converts into
electrical energy by the enzymes as selective catalysts. This concept has received significant attention
due to various advantages - low cost of components, mild reaction conditions, high specificity of
substrate and availability of membrane less system. In past years, we have focused on the enhancement
of performance in bioelectrode, and the enzyme electrodes are applied in the EFC system. In this
presentation, we would like to introduce the technologies that include development of bioelectrode,
enhancement of mediator performance, determination of reaction conditions, and utilization of
biomass hydrolysate as a fuel source. Especially, the hydrolysates from lignocellulosic biomass are
newly applied as a fuel source in our EFC system. These results provide a promising direction for
further development and it could be utilized for miniature electronic devices.
Keywords: Enzyme fuel cell, Power generation, Enzyme, Biomass
Dr. Seung Wook Kim is a current professor at the Department of Chemical and Biological
Engineering in Korea University. He received his master’s degree at Korea University and
gained his doctoral degree at The University of Birmingham. He had 213 papers (SCI/SCIE),
49 patents registered, and 4 books translated. He is currently a Senior member in the National
Academy of Engineering of Korea and a Board member of Bioprocess and Bioseparation
Engineering (BBE) Division of AFOB, The Korean Society for Biotechnology and
Bioengineering (KSBB), He sits at Editorial Board of J. Ind. Eng, Chem of KSIEC, Korean
Chem. Eng. Res. of KICHE, and Bioresources.
7

9th
DOST - PCIEERD
INNOVATIVE TECHNOLOGY OF BIOMASS CONVERSION INTO
VALUE-ADDED BIOPRODUCTS
Suraini Abd-Aziz1
*, Mohd Azwan Jenol1
, Ruqayyah Masran1
, Nahrul Hayawin Zainal1
, and
Mohamad Faizal Ibrahim1
1
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra
Malaysia, 43400, Serdang, Selangor, Malaysia.
*Email: suraini@upm.edu.my
Malaysia has been blessed with vast amount of tropical agricultural land as well as lots of crop
cultivation. Due to high demand in each sector, a lot of biomass associated to agricultural activities
have been produced. Biomass conversion technology is emerging as an innovative field in Malaysia
due to the increasing demand by the agricultural sector in reusing and recycling biomass into value
added bioproducts. Biomass produced from the agricultural activities were oil palm empty fruit bunch
(OPEFB) (18 million tonnes/year in 2016), oil palm decanter cake (OPDC) (2.1 million tonnes/year
in 2016), sago biomass (110 tons per day in 2009), rice straw (593.2 tonnes/year in 2011), lemongrass
leaves (5328 tonnes/year in 2012) and pineapple wastes (226,009 tonnes/year in 2015) has been proven
to be an excellent feedstock for production of high value product such as biofuels (biobutanol and
bioethanol) and other bioproducts (biovanillin, biochar, biosugars, biosurfactants, essential oils and
animal feed). Current technology used in biomass conversion into value added product involves
pretreatment process, saccharification, carbonization, extraction, separation, gasification in which the
efficient methods in producing the precursors will be selected. Biomass conversion technology is far
from its final destination where efficiency, productivity and revenue generated at its maximum
capacity, but still in its relative infancy. A lot of work needs to be done but the possibility, as stated
earlier, is endless. Further research and development in terms of novel method and analysis, in tandem
with proper implementation in social and economic sector can further increase the growth of biomass
conversion technology to its greater untapped potential.
Keywords: Innovative technology, Biomass conversion, Feedstocks, Biofuels, Value added bioproducts
Professor Dr. Suraini Abd-Aziz is currently the Deputy Dean (Academic and Student
Affairs) of the Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra
Malaysia. She graduated with a MSc (Biochemical Engineering) from University of Wales
Swansea, United Kingdom and PhD (Biochemical Engineering) from University of Wales
Swansea, United Kingdom. In research, she had developed her research interest in the field
of Industrial and Environmental Biotechnology. Apart from being the project leader of her
own research grants, she also actively contributed to other projects in her capacity to be
expert in enzyme technology, fermentation technology, bioprocess engineering and
environmental biotechnology. She had and currently supervised and co-supervised over 75
post-graduate students (PhD and MSc students). With h-Index of 20 (total citations of 1279),
she published more than 160 referred journals internationally.
8

9th
DOST - PCIEERD
Abstracts of
Oral Presentations
83

9th
DOST - PCIEERD
EFFECTS OF LIGHT CONDITIONS ON THE IN VITRO GROWTH OF
MICROALGAE NANNOCHLOROPSIS OCULATA
Cam Tu Trinh1
*, Thanh Huong Tran1
, and Trang Viet Bui1
1.
Plant Physysiology Department, Biology and Biotechnology Faculty, University of Science, Vietnam National
University -HCM
*Email: tctu@hcmus.edu.vn
Nannochloropsis oculata is considered as an extraordinary potential microalgae because of
high biomass concentration, lipid content, and fatty acid compositions which are suitable for the
production of biofuels. N. oculata was grown in modified f/2 medium at different photoperiods. One
drop of N.oculata cell suspension containing 3 to 5 unicells was put on the lame to observe cell division
from one chosen cell. A cell cycle of N. oculata last approximately 24 hours with three main phases:
mitosis, cytokinesis and cell release. In mitosis, cell nucleus divided from 1 to 8 at the 8th
to the 16th
hour. Cytokinesis began at the 12th
and last to the 19th
hour with the formation of 8 daughter cells
which were covered by mother cell wall. Finally, cells were separated from the 8-cell cluster in the
cell release phase during the 19th
to the 23th
hour. Cell density was often high at the beginning of light
period and low at the start of dark period. The decrease of cell density at the end of every light period
may be due to cell degeneration. Some tiny fragments of cells were observed at very low ratio in log
phase and higher in stationary phase. Morphological changes in cell divison and the roles of light on
growth rhythm of N. oculata were discussed.
Keywords: cell division, growth rhythm, light condition, microalgae culture, Nannochloropsis oculata,
10

9th
DOST - PCIEERD
FACTORS AFFECTING BIOCONVERSION OF REUTEALIS TRISPERMA
SEED BY BLACK SOLDIER FLY LARVAE FOR THE PRODUCTION OF
PROTEIN AND OIL-RICH BIOMASS
Muhammad Yusuf Abduh1
, Mochamad Hirza Nadia1
, Syaripudin1
*, and Robert Manurung2
1
Department of Bioengineering, School of Life Sciences and Technology, Institute of Technology of Bandung, Jalan
Ganesha 10, Bandung, 40132, Indonesia
2
Agrotechnology and Bioproduct Technology Research Group, Institute of Technology of Bandung, Jalan Ganesha 10,
Bandung, 40132, Indonesia
*E-mail: yusuf@sith.itb.ac.id
A systematic study on the use of Reutealis trisperma seed as a substrate for the cultivation of
black soldier fly larvae (Hermetia illucens) was performed. The characteristics of Reutealis trisperma
seed from two different locations particularly Papua and West Java were determined. The seed has a
relatively high oil (37.6-39.2 wt%, d.b.) and protein content (14.9-28.2 wt%, d.b.). The effect of shell
to cake ratio (0-0.25 wt%, w.b.), water to biomass ratio (1-2.3 wt%, w.b.), feeding rate (50-100
mg/larvae/d), lighting condition (dark-light) and larval height in a rearing container (4-10 cm) was
investigated. An optimum prepupal biomass productivity of 123.4 g/m2
.d was obtained (0.25 wt%,
w.b. cake to shell ratio, 1.5 wt%, w.b. water to biomass ratio, 100 mg/larvae/d, dark, 6 cm larval
height). The protein and oil content of the biomass were also determined to evaluate the potential of
Reutealis trisperma seed as a substrate for the cultivation of black soldier fly larvae to produce protein
and oil-rich biomass. The oil content in the biomass was also extracted and the fatty acid composition
was identified. The prepupal biomass has a relatively high amount of protein (45 wt%, d.b.) and oil
content (26.6 wt%, d.b.). The prepupae may find application as a high protein feed whereas the oil
extracted from the black soldier fly larvae may be utilized as a low cost biodiesel feedstock.
Keywords: Reutealis trisperma, Hermetia illucens, prepupal biomass productivity, protein and oil-rich biomas
11

9th
DOST - PCIEERD
ELUCIDATION OF THIAMINE (VITAMIN B1) BIOSYNTHESIS PATHWAY
IN GRACILARIA SP. AND THE EFFECT OF STRESS TOWARDS THIAMINE
BIOSYNTHESIS
Nur Sakinah Meor Mohd Ghazali1
and Zetty Norhana Balia Yusof1*
1
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400
UPM, Serdang, Selangor, Malaysia
*Email: zettynorhana@upm.edu.my
Thiamine or vitamin B1 is vital to maintain the proper functioning of all living organisms.
Thiamine pyrophosphate (TPP), is an important cofactor for several important enzymatic reaction and
also plays a role as a stress signaling molecule in response to environment changes. As they are not
synthesized in humans, thiamine is obtained from plant and animal sources. The major food source
rich in thiamine are grains, meat and fish. Seaweeds are plant-like organisms that have been proven to
have high thiamine content. However, the thiamine biosynthesis pathway in seaweeds is currently
underexplored. Moreover, seaweeds are not fully utilised as a thiamine source or in the production of
thiamine. In this study, Gracilaria changii, a red seaweed was explored as they are abundantly found
in Malaysian local environment and fast growing. This study aims to elucidate the thiamine
biosynthesis pathway in G. changii by amplifying gene transcripts coding for thiamine biosynthesis
enzymes. Primers have been designed to amplify all the genes involved in thiamine biosynthesis
pathway via polymerase chain reaction (PCR). Besides that, the hunt for TPP riboswitch motif(s) in
G. changii’s thiamine biosynthesis genes via bioinformatics and experimental approaches was also
carried out. A number of riboswitch-finding web servers were utilised namely RiboSW, RibEX,
Riboswitch Scanner, and Denison Riboswitch Detector. In addition to that, the expression of thiamine
biosynthesis genes was observed upon stress application. G. changii was laboratory-cultured and
abiotic stress was induced using various stressors. The expression of thiamine biosynthesis genes will
be quantified using quantitative polymerase chain reaction (qPCR). With knowledge of the genes
involved in thiamine biosynthesis pathway and also the stressors which could lead to overexpression
of thiamine in G. changii, manipulation of the pathway could be done for various purposes.
Keywords: Thiamine, Thiamine pyrophosphate Seaweeds, Riboswitch, Stress
12

9th
DOST - PCIEERD
BIOCONVERSION OF RUBBER SEED TO PRODUCE PROTEIN AND OIL-
RICH BIOMASS EMPLOYING BLACK SOLDIER FLY LARVAE ASSISTED
BY MICROBES
Muhammad Yusuf Abduh1*
, Maryam Jamilah1
, Pramesti Istiandari1
,
Mochamad Hirza Nadia1
, Syaripudin1
, and Robert Manurung1
1
Department of Bioengineering, School of Life Sciences and Technology, Institute of Technology of Bandung, Jalan
Ganesha 10, Bandung, 40132, Indonesia
Agrotechnology and Bioproduct Technology Research Group, Institute of Technology of Bandung, Jalan Ganesha 10,
Bandung, 40132, Indonesia
*E-mail: yusuf@sith.itb.ac.id
A co-conversion process using black soldier fly larvae (Hermetia illucens) and microbes was
studied to convert rubber seed into insect biomass containing high amount of protein and oil. De-oiled
rubber seeds (cake to shell ratio of 2.5 wt%) was mixed with water (water to biomass ratio of 1.5-2.3
wt%) and pre-treated with two types of microbes; liquid culture of Aspergillus niger (107
spore/ml)
and Biotaff solution containing consorsium of microbes (107
cell/ml). Black soldier fly larvae were
reared in rearing containers (6.8 x 6.8 cm) with a larval density of 5 larvae/cm2
and a feeding rate of
68 mg/larvae/d. The rearing containers were subjected with different shade rate (25-98%). After 12
days of treatment, the final average weight of the prepupae lies in the range of 7.2 to 19.5 g with an
estimated biomass productivity of 133 to 358 g/m2
.d. The efficiency of black soldier fly larvae in
converting the digested feed is 12.5 to 25.9 % with an assimilation efficiency of 22.9 to 28.7 %. The
waste reduction index is estimated at 2.1 to 2.6. The protein and oil content of the prepupae was
analysed to evaluate the potential of pre-tretated rubber seed as a substrate for the cultivation of black
soldier fly larvae to produce protein and oil-rich biomass. The prepupal biomass has a relatively high
protein (28.6-55.2 wt%, d.b.) and oil content (18.9 to 28.3 wt%, d.) and is suitable for application as
cattle feed.
Keywords: Hermetia illucens, rubber seed, Aspergillus niger, shade rate, biomass productivity
13

9th
DOST - PCIEERD
COMPARISON OF YIELD OF REDUCING SUGAR OBTAINED FROM
HYDROTHERMAL AND ALKALI PRETREATED BROWN SEAWEED
“SARGASSUM SP.”
Kyaw Wunna1
*, Joseph Auresenia1
, Leonila Abella1
, Pag-asa Gaspillo1
, Kiyohiko Nakasaki2
1
Chemical Engineering Department, De La Salle University, Philippines, (+632) 524 4611 Ext. 243
2
Department of International Development Engineering, Tokyo Institute of Technology, 2 Chome-12-1 Ookayama,
Meguro, Tokyo 152-8550, Japan
Email: kyawwunna550@gmail.com
Macroalgae, found as various species, are becoming potential feedstock in bioethanol
production due to the rich in carbohydrate and low in lignin. In this study, Sargassum sp. was treated
with hydrothermal and alkali methods to increase sugar content in macroagale. In alkali pretreatment,
concentration of sodium hydroxide (0.5- 2%w/v.) and residence time (10- 90 min) were varied at
121°C with two different solid loading (10%w/v and 20%w/v). Hydrothermal treatment of macroalgae
was also performed at the same solid loadings and same temperature with various residence times.
The pretreated dried samples were analyzed in term of reducing sugar and lignin contents. High sugar
concentrations were obtained at NaOH 1-1.5%w/v. By comparison of two methods, alkali
pretreatment can increase the concentration of reducing sugar and decrease the lignin more than
hydrothermal pretreatment.
Keywords: Macroalgae, Sargassum sp., hydrothermal treatment, alkali pretreatment
14

9th
DOST - PCIEERD
A REVIEW ON ABACA FIBER REINFORCED COMPOSITES
Joefreim Delicano1
*
1
Graduate Student, Doctor of Philosophy in Technology Management, Technological University of the Philippines,
Manila
1
Faculty, Department of Aeronautical Engineering and Aircraft Maintenance Technology, FEATI University
*Email: joefreim@yahoo.com
This paper reviews literatures and information on Abaca fibers (Musa textilis Nee) as
reinforcing material for composites. Characterization and sources of Abaca as well properties of Abaca
reinforced composites and its applications were discussed. Therefore, challenges and future works for
Abaca Reinforced composites were explored. According to the Fiber Utilization and Technology
Division of the Philippine Fiber Industry Development Authority, the focus have been given on using
less subscribed fibers and trimmings from Abaca fiber plants as reinforcing material for composites
of various industrial applications. Studies also reveal that Abaca fiber pre-treatment helps in improving
the mechanical properties of the composite. In addition, there have been efforts in combining Abaca
fibers to existing mixture of synthetic composites to improve its environmental performance. The
future of Abaca is seen as one of the potent sources of reinforcing fiber for various material
construction including aerospace materials.
Keywords: Abaca, Manila Hemp, Musa Textilis Nee, Natural Fiber Reinforced Composites, Comp
15

9th
DOST - PCIEERD
MICROALGAL LIPID EXTRACTION BY SUPERCRITICAL CARBON
DIOXIDE AND ITS CONVERSION TO FATTY ACID METHYL ESTER
Alva Durian1
*, Maria Cristina Macawile1
, Jurex Gallo1
and Joseph Auresenia1
1
Chemical Engineering Department, Gokongwei College of Engineering, De La Salle University, 2401 Taft Avenue,
Malate, Manila, 1004 Metro Manila, Philippines
*Email: alva_durian@dlsu.edu.ph
Algae have been a promising feedstock for several applications such as bioplastics, animal
feeds, fertilizer, pharmaceuticals, cosmetics, and biofuel. Studies were done to convert microalgae to
biogas, bioethanol, biobutanol, and biodiesel as alternative sources of energy from the existing and
depleting fossil fuels. Triglycerides (TGs) extracted from microalgae were usually converted to fatty
acid methyl ester (FAME) or biodiesel. However, conventional method of TG extraction such as
chemical and mechanical treatments use hazardous solvent and requires high amount of energy.
Further, these method needs further process to separate TGs from solvent or cell debris. In order to
address these concern, “green” technology such as supercritical CO2 (SCCO2) method was used to
efficiently extract desirable triglycerides for biodiesel conversion.
In this study, factors such as CO2 flow rate, pressure of the system, and temperature of the
reaction were varied on SCCO2 extraction. Then the extracted TGs were converted to FAME by
transesterification method. Results showed that pressure mainly affects extraction efficiency. Based
from the FAME profile tested on gas chromatography – flame ionization detector (GC-FID), algae
sample contain myristic acid (C14:0), pentadecylic acid (C15:0), palmitic acid (C16:0), stearic acid
(C18:0), arachidic acid (C20:0), heneicosylic acid (C21:0), behenic acid (C22:0), and its
corresponding unsaturated fatty acids.
Keywords: Supercritical carbon dioxide, Extraction, Fatty acid methyl ester, Algae
16

9th
DOST - PCIEERD
EXTRACTION OF KENAF SEED OIL AND KAKAWATE SEED OIL USING
SUPERCRITICAL CARBON DIOXIDE
Maria Cristina Macawile1
*, Alva Durian1
, Jurex Gallo1
, Luis Razon1
,
Raymond Girard Tan1
, and Joseph Auresenia1
1
Malate, Manila, 1004 Metro Manila, Philippines
*Email: macawile.cris@gmail.com
The use of second generation feedstock has shown potential to substitute the traditional edible
food crops for the production of biodiesel. Kenaf (Hibiscus cannabinus L.) and kakawate (Glircidia
Sepium) methyl esters were successfully produced by conventional extraction procedure and base
catalyzed transesterification process. The present study sought to evaluate the technical viability of
using supercritical carbon dioxide to extract oil from these non-edible seeds. The performance in
terms of % oil yield was compared to chemical solvent extraction using n hexane and conventional
Soxhlet extraction process. The chemical composition of kenaf and kakawate biodiesels were
analysed using gas chromatography – mass spectroscopy (GC-MS). Using kenaf seeds as source of
biodiesel the following methyl esters were found C16:0 methyl palmitate, C18:0 methyl stearate ,
C18:1 methyl oleate , C18:2 methyl linoleate while kakawate biodiesel contains C16:1 methyl
palmitoleate , C16:0, C14:0 methyl palmitate, C18:0 methyl stearate , C18:1 methyl oleate and C18:2
methyl linoleate . Other physical and chemical properties of oil and biodiesel were analyzed and
compared to ASTM D6751 or the EN14214 biodiesel standards.
Keywords: Supercritical, Kenaf, Kakawate, scCO2, biodiesel
17

9th
DOST - PCIEERD
TRANSDERMAL CANCER VACCINE BY SOLID-IN-OIL(S/O)
NANODISPERSIONS
Masahiro Goto1,2,3
*, Masato Sakuragi1
, Rie Wakabayashi1,3
, and Noriho Kamiya1,2,3
1
Department of Applied Chemistry, School of Engineering,
2
Center for Future Chemistry,
3
Center for Advanced Transdermal Drug Delivery Systems, Kyushu University
744 Motooka, Fukuoka 819-0395, Japan
*E-mail: m-goto@mail.cstm.kyushu-u.ac.jp
Cancer vaccine has recently received much attention due to its low risk of side effects. In this
study, we focused on the transcutaneous drug delivery system. Compared with conventional injection,
transcutaneous delivery is an ideal drug administration method because it is noninvasive, safe and
simple. Furthermore, it is known that the skin contains an abundance of immune cells. Therefore, we
expected that an effective anti-cancer immunity could be induced by the transcutaneous cancer vaccine.
However, the outermost, hydrophobic layer of skin, the stratum corneum, hinders penetration of
antigens into the skin. To realize effective transcutaneous delivery of antigens, we have developed a
solid-in-oil (S/O) technique that produces an oil dispersion of hydrophilic biomolecules.
To overcome this problem, we have developed the Solid-in-Oil (S/O) technique. The S/O
technique prepares oil-based nanodispersions of hydrophilic biomolecules by coating them with
hydrophobic surfactants. Because hydrophobic materials are more permeable through SC than
hydrophilic ones, the delivery of antigens into the skin can be improved by the S/O technique. We chose
the melanoma, a kind of the skin cancer, to evaluate the cancer immunity. Because a melanoma
antigen, TRP-2 peptide, has low solubility in water, we added lysine residues to the N-terminus of the
peptide and the newly developed peptide antigen, K-TRP-2, was used as a melanoma antigen. In
addition, we tried the use of R-848 as hydrophobic adjuvant to induce stronger immunity. The in vivo
experiment revealed the effective prevention of tumor and the metastasis was achieved by S/O
formulation containing R-848.
Keywords: DDS, cancer vaccine, immunotherapy, S/O technique
18

9th
DOST - PCIEERD
DESIGN OF NOVEL BIOCATALYSTS BY ENZYMATIC BIOMOLECULAR
CONJUGATION
Noriho Kamiya 1,2
*, Mari Takahara1
, Rie Wakabayashi1
and Masahiro Goto 1,2
1
Department of Applied Chemistry, School of Engineering, Kyushu University
2
Division of Biotechnology, Center for Future Chemistry, Kyushu University
744 Motooka, Fukuoka 819-0395, Japan
*E-mail: nori_kamiya@mail.cstm.kyushu-u.ac.jp
Designing new bioconjugates are of great interest in biological research and related applications.
The combination of unique functionalities of major biological components, nucleic acids and proteins
has been demonstrated to be an effective approach to create novel bioconjugates. Specifically, a DNA
aptamer, an artificial single-strand DNA whose functions can be evolved to suit a function of interest, is
an ideal element of such biohybrid molecules. Herein, we describe the potential of a DNA aptamer as
an element in biocatalysis at a solid surface. We selected CelApt, a crystalline cellulose-binding DNA
aptamer, for conjugation and cellulose degradation reaction. Then it was site-specifically crosslinked to
the C-terminus of the catalytic domain of endoglucanase by our original two-step enzymatic conjugation
strategy, to mimic natural endoglucanase.
Another type of conjugates can be designed by the combination of naturally occurring
biomolecules and synthetic materials. Protein-polymer hybrids have thus been developed in many
research and industrial fields to expand the use of protein functions or to realize novel properties of
hybrids. Currently, most reported hybrids are composed of one protein attached to one or several
polymer chains. The other form of hybrids, multiple protein assemblies on one polymer chain. However,
the preparation method for this kind of hybrid is limited because it requires precise control of the reaction
site on both proteins and polymers to prevent random cross-linking. Herein, an enzymatic method has
been developed to solve this problem, and potential application of the resultant new bioconjugates has
been demonstrated in an immunosorbent assay.
Keywords: Aptamer, bioconjugate, cellulase, immunoassay, microbial transglutaminase
19

9th
DOST - PCIEERD
MICROWAVE-ASSISTED GRAPHENE OXIDE CATALYSIS FOR
CASCADE SYNTHESIS OF ADVANCED BIOFUEL
FROM WASTE OIL
Yoshifumi Sumigawa1
, Armando Quitain1*
, Mitsuru Sasaki2
, and Tetsuya Kida1
1.
Graduate School of Science and Technology, Kumamoto University Department, 2-39-1, Kurokami, Kumamoto, 860-
0862, Japan.
2.
Institute of Pulsed Power Science, Kumamoto University, 2-39-1, Kurokami, Kumamoto, 860-0862, Japan.
*
E-mail:quitain@kumamoto-u.ac.jp
Biodiesel (BDF), the fatty acid methyl esters (FAMEs), has attracted increased attention
recently as an alternative to petroleum diesel. FAMEs are synthesized either from the esterification of
fatty acids in the presence of conventional homogenous acid catalysts (e.g. H2SO4) or by the
transesterification of vegetable oils using basic catalysts (e.g. NaOH). Generally, for every 3 mol of
methyl ester produced, 1 mol of glycerin is synthesized, which is nearly 10 wt% of total product. One
of the major obstacles to the commercialization of biodiesel is its inevitable co-product glycerin’s
market becoming saturated, causing a dramatic reduction in prices and consequently affecting the
biodiesel economy adversely. Furthermore, there are some disadvantages in the conventional
production process such as homogeneous catalysts are being used and long reaction time. In this study,
as a replacement to homogeneous catalyst, we proposed the use of graphene oxide (GO), a carbon-
based heterogeneous catalyst. In addition, the use of microwave heating with inherent internal and
localized heating as an alternative to conventional heating method could facilitate shorter reaction
time. GO based catalyst has high microwave absorptivity, thus, it can be considered that the use of
GO based catalyst under microwave heating would be a novel approach for an efficient synthesis of
advanced biofuel. As a result, highest esterification yields of 99.6 % was obtained in less than 5 min
of microwave irradiation. The highest glycerin conversion of 51.1 % was obtained, which was better
than that of the conventional heating method.
Keywords: Graphene oxide, Microwave, Biofuel, Esterification, Etherification
20

9th
DOST - PCIEERD
DETERMINATION OF ANTIFUNGAL ACTIVITIES FROM MALAYSIAN
SEAWEED EXTRACTS AGAINST GANODERMA BONINENSE, A DISEASE-
CAUSING FUNGUS IN OIL PALM (Elaies guineensis)
Syamimi Diyana Binti Abdul Aziz1
* and Zetty Norhana Balia Yusof1
1
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400
UPM, Serdang, Selangor, Malaysia.
*Email: mimi_diyana93@hotmail.co.uk
As a major contributor of the world’s edible vegetable oil and a source of biofuel in Malaysia,
oil palm (Elaies guineensis) is one of the most important crops in Malaysia. However, the productivity
of this golden crop is hampered by basal stem rot (BSR) disease which is caused by a fungus,
Ganoderma boninense. To date, no effective control measure is available and this situation needs
attention urgently. Seaweeds are plant-like marine organisms which are known to be rich of many
useful compounds like antioxidants, antibacterial and other useful bioactive compounds and they grow
naturally abundantly along the Malaysian coastline. However, antifungal potential in Malaysian
seaweeds are still underexplored. In this project, four seaweeds namely Caulerpa racemosa, Caulerpa
microphysa, Sargassum duplicatum and Halimeda opuntia collected from Teluk Kemang, Port
Dickson, Malaysia (2°26′ N,101°51′ E) were screened for their antifungal activities. The seaweeds
were extracted with four different types of solvents which are dichloromethane, methanol, chloroform
and aqueous. Methanol extracts showed the highest yield percentage of 19.92% from the initial weight
followed by aqueous 14.35%, dichloromethane 7.82% and chloroform 4.85%. The antifungal
activities were screened using poisoned food technique against oil palm fungal pathogen, G. boninense
with three different concentrations of seaweed extract (5, 10 and 20 µl/ml). S. duplicatum and C.
microphysa dichloromethane extracts at 5 and 10 µl/ml concentration showed significant antifungal
activity against G. boninense with inhibition percentage of 63.1% and 71.4% respectively. The
findings suggested that local Malaysian seaweed species have high potential as a source of antifungal
compounds which could be useful specifically for the application in the oil palm industry.
Keywords: Seaweeds, fungus, oil palm, antifungal activity, bioactive compounds
21

9th
DOST - PCIEERD
EFFECT OF DIFFERENT EXTRACTION CONDITIONS ON THE YIELD OF
PECTIN EXTRACTED FROM BILIMBI (Averrhoa bilimbi)
Reygan H. Sangalang1
*
1
College of Arts and Sciences, Batangas State University,
Batangas City, 4201, Batangas, Philippines
*Email: rhsangalang@gmail.com
Pectin is a naturally occurring biopolymer that is widely used in food industry, pharmaceutical
industry and biotechnology. Pectin from bilimbi (Averrhoa bilimbi) was extracted under different
extraction conditions. The optimization of pectin extraction was done by varying the extraction time
(30 min, 60 min and 120 mins), pH (1.5, 2.0 and 2.5) and temperature (70°C, 80°C and 90°C). The
highest yield of pectin was observed after 120 minutes of extraction at pH 2.0 and extraction
temperature of 90°C. Statistical analysis indicated that variations of temperature, pH and extraction
time had significant effects on the yield of pectin.
Keywords: Bilimbi, Extraction, Optimization, Pectin, Yield
22

9th
DOST - PCIEERD
PRELIMINARY STUDY OF THE PRODUCTION OF
PHYCOBILIPROTEINS FROM GRACILARIA CHANGII
Rugi Vicente Rubi1
, Erison Roque1
*, Fritzzie Gutierrez1
, May Anne Hegin1
,
Jeanell Martin1
, Arrianne Jaye Mata1
, and Ervin Mission1
1
Department of Chemical Engineering, Adamson University, Manila, Philippines
*Email: erison.roque@adamson.edu.ph
This paper presented the potential of Gracilaria changii, a red macro-algae found in the
Philippines as a source of Phycobiliproteins (PBP). The extraction process was done using Acetic
acid-Sodium acetate buffer solution and was followed by purification using Ammonium sulfate
precipitation and dialysis with Sodium Phosphate buffer solution (NaPi). Both the extracted and
purified PBP solution undergone centrifugation at 3, 5, and 7 minutes time variation and were
subjected to UV-Vis Spectrophotometer for concentration quantification at wavelength range of 400-
700 nm for the three PBPs composition: Phycoerythrin (PE), Phycocyanin (PC) and Allophycocyanin
(APC), respectively. Results showed that the highest concentration of 27.32 µg/ml for APC, 15.90
µg/ml for PC and 23.71 µg/ml for PE were obtained at the 5 minute- extraction process. One-way
ANOVA showed that there was a significant effect of time on extracted PBP concentrations at the
p<0.05 level [F (2) = 9.52, p = 0.014] while an insignificant effect on purified phycobiliprotein
concentration at the p>0.05 level [F (2) = 0.75, p = 0.513].Taken together, these results suggest that
centrifugation time variations have significant effect on PBP concentration in the extracted and none
on the purified part.
Keywords: Red macro-algae, Phycobiliprotein,Centrifugation, Extraction, Purification
23

9th
DOST - PCIEERD
AN UNIQUE NICOTINE-DEGRADING BACTERIUM REVEALED BY
MULTI-HIERARCHY ANALYSIS
Jun Li1
, Lie Xiong1
, Yang Jiao1
, Houlong He1
, Weihong Zhong1
*
1
College of Biological Engineering, Zhejiang University of Technology, Hangzhou 310014, PR China
*E-mail: whzhong@zjut.edu.cn
Microbial catabolism plays a significant role in the dissipation of nicotine residues in the
environment. In the present study, an efficient nicotine-degrading strain JY-Q that nearly complete
degradation was achieved within 24 h at 5.0 mg/ml nicotine, was isolated and identified as
Pseudomonas sp. in Hangzhou, China. The optimal culture conditions of strain JY-Q for nicotine
degradation were 37°C and pH 7.0 in the basic salt medium. However, the strain showed strong
endurance against 10.0 mg/ml nicotine. Strain JY-Q degrades nicotine through a putative novel
mechanism revealed via genomics, transcriptomics and metabolomics analysis. Surprisingly, three
candidate gene clusters located separated in distant loci were predicted to confer nicotine degradation.
One is the nicotinic acid degradation loci (AA098_17770 - AA098_17790) and the others are the
nicotine degradation locis (AA098_21325 - AA098_21340, AA098_03885 - AA098_ 03900). They
exhibited inconsistent gene organizations and expression behaviors. They might be relative to the
‘dynamic’ genome of strain JY-Q. In addition, the elucidation of the nicotine degradation pathway for
Pseudomonas sp. JY-Q provides and enhances potential application in bioremediation of nicotine-
contaminated environments, such as tobacco waste extracts.
Keywords: Pseudomonas sp. JY-Q, nicotine-degrading, genomics analysis, dynamic genome
24

9th
DOST - PCIEERD
SELECTIVITY CYTOTOXICITY OF BIOLUMINESCENT BACTERIA
ISOLATED FROM FRESH INK OF PHILIPPINE SQUID ON HUMAN
COLON CANCER AND NORMAL CELL LINES
Jocelyn Luyon 1,2
*, Glenn Oyong 2
, and Esperanza Cabrera 2
1
Environmental Resource Management Center, De La Salle University-Dasmariñas, DBB-B City of Dasmariñas, 4115,
Cavite, Philippines
2
Biology Department, College of Science, and Center for Natural Science and Environmental Research, De La Salle
University, 2401 Taft Avenue, 1004 Manila, Philippines
*Email: jpluyon@dlsud.edu.ph
The current work determined the cytotoxicity of the symbiont bioluminescent bacteria isolated
from fresh ink of the squid, Photololigo duvaucelii for human colon cancer cell lines HT-29 and
HCT116 and normal human dermal fibroblasts.
The crude sterile culture supernatants of the bacterial isolates grown in marine broth containing
2.8% of NaCl were tested for their cytotoxic activities for HT-29 and HCT-116 human colon cancer
cells and the normal human dermal fibroblasts HDFn using Presto Blue™ Resazurin Assay. Zeocin
served as the positive control. The cytotoxicity index profiles of all culture supernatants and negative
control (marine broth with 2.8% NaCl) for HDFn suggest non-toxicity to the cells, whereas most
culture supernatants were observed to be cytotoxic to the two colon cancer cell lines. The culture
supernatants of the isolates were found to be more cytotoxic for the HT-29 colon cancer cells than to
the HCT-116 colon cancer cells. At the same time, the IC50 values showed that 85% (17/20) and 45%
(9/20) of the culture supernatants tested were significantly lower, hence more potent, than zeocin for
HT-29 and HCT-116, respectively (p<0.05). The rest were equally potent (p>0.05). 16S rRNA gene
sequence analysis of the bioluminescent isolates studied revealed that they have a 97-99% similarity
identity with Photobacterium leiognathi.
This may be the first report on the cytotoxic activities on cancer cells of P. leiognathi from the
Philippine squid, and suggests the potential use of the secondary metabolites of these bioluminescent
bacteria as anti-cancer agents.
Keywords: Bioluminescent bacteria, cytotoxicity, secondary metabolites, squid ink, and colon cancer
25

9th
DOST - PCIEERD
EFFECT OF MONOETHYLAMINE CONCENTRATION ON BIOMASS
GROWTH, CO2 FIXATION AND FATTY ACID PROFILE OF CHLORELLA
SP AND THALASIORISA SP. USING TWO (2) OPERATIONS IN A FLASK
FLAT PLATE PHOTOBIOREACTOR
Racquel Ranchie Ting1
, Jose Simon Simangan1
, Alyzza Marie Narciso1
, and
Joseph Auresenia1
*
1
1004 Manila, Philippines
*Email:joseph.auresenia@dlsu.edu.ph
Microalgae as a source of biodiesel have been a favorite topic of renewable energy. It has been
acknowledge as a feasible option for biodiesel production due to its quick production rate and high oil
content. Though it also has the advantage of CO2 consumption, it is yet to become the mainstream
source of biodiesel because of insufficient knowledge on optimizing large scale algae production. It is
with this reason in mind, that the research is conducted to find more optimal conditions for biodiesel
production from microalgae. This study will be conducted using a flat-plate photobioreactor operated
simultaneously and sequentially. The concentration of fresh and used monethanolamine via
concentration ranging from 0-300 ppm will be the condition to be varied. The effect on the biomass
growth, FAME profile and carbon dioxide consumption of Thalassiosira Sp. and Chlorella Sp. will be
the paramaters to be tested in this study. The growth shall be observed daily with the use of Shimadzu
UV-Vis equipment and the FAME profile shall be tested with the use of the Clarus-500 Gas
Chromatography equipment. Should the results prove to be favorable, it can help further promote algae
as a source of biodiesel coupled with sequestration of carbon dioxide.
26

9th
DOST - PCIEERD
ENZYMATIC SACCHARIFICATION ENHANCEMENT BY
MORPHOLOGICAL AND STRUCTURAL MODIFICATION OF RICE HUSK
SUBJECTED TO HIGH PRESSURE STEAM PRETREATMENT
Muinat Olanike Kazeem1,2
*, Umi Kalsom Md Shah1
, Azhari Samsu Baharuddin3
, and
Nor’ Aini Abdul Rahman1
1
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra
Malaysia, 43400 Serdang, Selangor, Malaysia.
2
Department of Microbiology, Faculty of Life Sciences, University of Ilorin, 1515, Ilorin, Kwara State, Nigeria.
3
Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia.43400 UPM,
Serdang.Selangor, Malaysia
*E-mail: kazeemmuinat@gmail.com
The suitable pretreatment of agro-waste material for enzymatic saccharification is an important
step to reduce environmental pollution cause by chemical pretreatment. This study aimed to propose
a potential operational conditions for high pressure steam pretreatment (HPSP) of rice husk and to
explore the effect of morphological and structural properties of pretreated rice husk on enzymatic
saccharification. The effect of HPSP was successfully examined on rice husk at pretreatment
temperature of 160 °C, 180 °C, 200 °C, 220 °C for 2-10 min. Significant changes in pH of hydrolysate,
weight loss and compositional analysis of rice husk samples were observed after pretreatment. The
scanning electron microscopy (SEM) and Energy Dispersion X-ray (EDX) revealed modification in
morphological surface structure and variation in elemental composition of rice husk after pretreatment.
Degradation of hemicellulose and changes in functional group of lignocellulosic component were
identified using Fourier Transform Infrared (FTIR) and the crystallinity of samples was detected
through X-ray Diffraction (XRD). An optimum pretreatment temperature at 200 °C for 7 min treatment
time was found to effectively enhanced the reducing sugar yield and saccharification percentage after
enzymatic hydrolysis with cellulase from Bacillus licheniformis 2D55. Reducing sugar concentration
increased to 17.15 g/L at a saccharification percentage of 43.73 % after 24 h which was 2.5 fold higher
compared to untreated rice husk. This results suggest that high pressure steam is feasible for the
pretreatment of rice husk for fermentable sugar production and may also reduce environmental
pollution caused by chemical pretreatment.
Keywords: High pressure steam pretreatment; enzymatic saccharification;, rice husk; structural and morphological
characterization; fermentable sugar
27

9th
DOST - PCIEERD
CHARACTERIZATION OF METABOLIC PATHWAYS IN MARINE
MICROALGAE THROUGH IN SILICO MODEL
Seong-Joo Hong1
, Byung-Kwan Cho2
, Dong-Myung Kim3
, Hookeun Lee4
, Hyung-Kyoon Choi5
, and
Choul-Gyun Lee1
*
1
Department of Biological Engineering, Inha University, Incheon, Republic of Korea,
2
Department of Biological Sciences, KAIST, Daejeon, Republic of Korea
3
Department of Fine Chemical Engineering and Applied Chemistry, Chungnam University, Daejeon, Korea
4
College of Pharmacy, Gachon University, Incheon, Republic of Korea
5
College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea
*Email: leecg@inha.ac.kr
Eukaryotic microalgae are attracting a lot of attention due to their potential for sustainable
production of biofuel precursors such as lipids, starches, alcohols and hydrogen gas. In addition, they
can convert solar energy into biomass using carbon dioxide by photosynthesis. Recently various
attempts in biological fields have been made to produce microalgal biofuel. Especially since genome
database of Chlamydomonas reinhardtii was published in 2007, high throughput omics data of
eukaryotic microalgae have been accumulating at a rapid rate. Among omics data, genomics and
transcriptomics researches are developing rapidly by development of NGS technologies. Over 30
whole genome sequencing of eukaryotic microalgae were in progress or completed in the JGI (Joint
Genome Institute) and transcriptome of Dunaliella tertiolecta and Nannochloropsis gaditana was
characterized by NGS (next generation sequencing). With accumulation of these enormous data,
macroscopic view of cellular network has been required requisitely. To date as the one of research
tools to gather and analyze huge information, systems biology is worth of remark. Through the use of
systems biology, molecular and cellular phenomena of eukaryotic microalgae could be modeled using
integrated and interacting network of genes, transcripts, proteins and biochemical reactions under
stressful conditions.
Fatty acid contents and carbohydrate contents were observed to be differentially regulated in
microalgae under nitrogen depleted condition. To characterize fatty acid accumulation mechanism,
genome data was used as a frame work for metabolic reconstruction. Transcriptome, proteome and
metabolome analysis of the cells were performed and integrated to reconstruct cellular network in
eukaryotic microalgae. To enhance lipid productivity from microalgae, in silico model of eukaryotic
microalgae was reconstructed based on OMICS data. It can make us fully understand the lipid
accumulation mechanism within eukaryotic microalgae. This in silico analysis integrated multi-omics
data could become the driving force for exploiting metabolic and regulatory mechanisms to improve
microalgal cells as a biofuel producer.
28

9th
DOST - PCIEERD
FORMULATION AND IN VITRO EVALUATION OF SURFACE-COATED
NANOCARRIER FOR ETOPOSIDE
Czarina Dominique Rodriguez1
, Rie Wakabayashi1
, Noriho Kamiya1,2
, and Masahiro Goto1,2
*
1
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
2
Center for Advanced Transdermal Drug Delivery Systems, Kyushu University
744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan
Etoposide, an anticancer drug that works by inhibiting topoisomerase II, is classified as poorly
soluble and poorly permeable drugs (Class IV) under the Biopharmaceutic Classification System
(BCS). Because of this property, the current parenteral formulation of etoposide contains surfactants
and co-solvents, such as Tween 80, PEG 300, and ethanol, to improve its solubility. However, some
of these were reported to cause adverse effects. To improve the formulation, a nanodelivery system
was formulated using the Solid-in-Water (S/W) technique. Nanodelivery systems for anticancer drugs
offer many advantages, most important of which is the enhanced permeation and retention (EPR)
effect, thus selectively targeting tumors and not affecting normal cells. In addition, the S/W technique
involves easy and simple emulsification and freeze drying processes. In this study, Pluronic F127, a
biocompatible triblock copolymer surfactant containing PEO-PPO-PEO groups, was used to formulate
a surface-coated nanocarrier for etoposide, thus rendering it readily dispersible in aqueous
environment. With 4:1 drug:surfactant ratio, spherical nanoparticles 150-200 nm in diameter were
produced, as characterized by DLS and TEM analysis. In vitro release study showed slow release of
the drug within 12 hours, and also suggests approximately ≥80% encapsulation efficiency. Cytotoxic
activity was also evaluated against B16F10 cells using WST assay, and the formulation exhibited
anticancer effect comparable to that of free etoposide.
Keywords: DDS, etoposide, Pluronic, nanocarrier, surface-coating
2729

9th
DOST - PCIEERD
TRANSCUTANEOUS IMMUNOTHERAPY FOR POLLINOSIS
USING SOLID-IN-OIL NANODISPERSION
Qingliang Kong1
, Momoko Kitaoka1
, Rie Wakabayashi1
, Noriho Kamiya1, 2
, and
Masahiro Goto1, 2
*
1
Department of Applied Chemistry, Kyushu University, Fukuoka, 819-0382, Japan
2
Center for Future Chemistry, Kyushu University, Fukuoka, 819-0382, Japan
Cedar pollinosis is a common disease posing a major health problem in Japan. However,
symptomatic therapy does not meet patients’ requirements for pollinosis. Therefore, we aimed to
develop an immunotherapy method using T cell epitope peptides with transcutaneous route.
T cell epitope peptides can induce T cells inactivation but do not cause side effects.
They are expected to cure pollinosis. Moreover, easy and convenient transcutaneous administration is
attractive. However, the topmost layer of skin, stratum corneum (SC), has a hydrophobic barrier
function. To overcome the barrier, we introduced the Solidin-Oil (S/O) technique based on
nanodispersion of hydrophilic drugs coated with hydrophobic surfactant molecules in an oil vehicle.
The hydrophobic solvent and the nano-order particles allow drugs to permeate through the SC.
Firstly, S/O nanodispersion was prepared. Hydrophilic peptides could be dispersed into the oil
successfully. Moreover, skin permeation efficiency in vitro was evaluated. Peptides permeated across
the skin successfully and peptide concentration in the skin was increased by 5 times after using S/O
nanodispersion. Finally, transcutaneous immunization in vivo was carried on. The S/O nanodispersion
could reduce the IgEmediated allergic reactions compared with aqueous solution.
S/O nanodispersion with T cell epitope peptides could be used for the treatment of
pollinosis.
Keywords: Transcutaneous immunotherapy, Nanodispersion, Pollinosis, Drug delivery, Peptide
30

9th
DOST - PCIEERD
RUBBER SEED OIL EXTRACTION FOR BIODIESEL PRODUCTION BY
LIQUIFIED DIMETHYL ETHER
Panatpong Boonnoun1
*, Pamornrat Chantam1
, Watanachai Yaowarat1
and Artiwan Shotipruk2
1.
Department of Industrial Engineering, Faculty of Engineering, Naresuan University, Phitsanulok 65000, Thailand
2.
Chemical Engineering Research Unit for Value Adding of Bioresources, Department of Chemical Engineering,
Faculty of Engineering, Chulalongkorn University, Phayathai Road, Bangkok 10330, Thailand
*Email: panatpong_p@hotmail.com
Rubber seed is produced from rubber tree which is mainly grown in Indonesia, Malaysia, Liberia,
India, Srilanka, Sarawak, and Thailand. The tree produces seeds weighing from 2 to 4 g which contains
approximately 40-60 weight% of non-edible oil that do not currently have any major industrial
applications.This work is thus focusing on the extraction of rubber seed oil (RSO) for biodiesel production
using liquefied dimethyl ether (DME) which is the potential solvent for oil extraction. By using DME as
extractant, the removal of solvent residue can be easily achieved by de-pressurized the system. The
extraction parameters including %moisture content in seed sample, solvent to sample weight ratio (w/w)
and extraction temperature on extracted rubber seed oil yield were studied. For the suitable condition at
40% moisture content, 6/1 solvent to sample weight ratio (w/w) and 35°C, approximately 46% extracted
oil yield can be obtained. The obtained rubber seed oil was analyzed the fatty acid composition by GC
analysis and it revealed that oil contained 17.19% linolenic, 39.50% linoleic, 26.10% oleic and
17.21% saturated fatty acid.
Keywords: Extraction, Liquefied dimethyl ether, Rubber seed oil, biodiesel.
31

9th
DOST - PCIEERD
SYNTHESIS OF CNT FOR PET BOTTLES, SARGASSUM USING
SIMULTANEOUS MICROWAVE ASSISTED PYROLYSIS AND
MICROWAVE PLASMA – CHEMICAL VAPOR DEPOSITION
Jan Patrick Si1
, Mark Angelo Tionson1
, Francis Ian Tobias1
*
1
The production of carbon nanotubes have been a commonplace topic for many studies in the
field of material science mainly due to its strong potential in the field of electronics, biotechnology,
medicine and manufacturing. In the past, carbon precursors from non-renewable, petroleum-derived
sources have been used to produce high-yield CNTs of varying structures and morphologies. However,
other novel sources which do not rely on depleting fossil fuels have not been extensively studied. This
paper would delve into the use of alternative carbon sources from PET bottles, Sargassum Polycystum,
and coconut husks in the synthesis of high-yield CNTs. A simultaneous microwave assisted pyrolysis
and microwave plasma enhanced chemical vapor deposition method will be employed to synthesize
the CNTs. The study would compare the effects of varying the combinations of the carbon sources,
catalysts (iron, nickel, cobalt) substrates (aluminum, silicon wafer and graphite) and microwave power
(7, 8 and 9) to the CNT yield and properties. After every run, the composition of the effluent gases
from the reactor shall be analyzed using a Clarus-500 Gas Chromatography equipment while the
average length, diameter and aspect ratio of the nanotubes would be determined using a JEOL JSM-
5310 scanning electron microscope. Purity of the obtained product would be measured through
thermogravimetric analysis. The results would be significant in identifying the optimal parameters for
possible large scale production of CNTs from sustainable resources.
32

9th
DOST - PCIEERD
PERFORMANCE OPTIMIZATION OF DSSC BY VARYING SENSITIZED
DYE RATIOS METAL DOPANT IN PHOTODIODE SENSITIZER, DYE
ACIDITY AND TYPE OF STABILITY CO-PIGMENT
Maria Eliza Cleofas1
, Ginelle Galido1
, Eulene Geronimo1
1
Dye sensitized solar cells (DSSC) invented by Gratzel and O’Regan are one of the emerging
photovoltaic (PV) innovations which provide low cost, non-toxic and efficient energy. While modern
solar cells have reached up to 30% efficiency, some of its materials are costly and energy consuming
due to purification processes in its production. With the use of alternatives, DSSCs provide a
promising future for solar energy. This research ventures into cheap natural dyes as substitutes to
expensive ruthenium complexes. This study aims to determine the best combination ratio of the
natural anthocyanin-containing dyes that will give the highest light conversion efficiency, compare
the best performance DSSC obtained with the performance of the inorganic one fabricated and tested
for the same applicable parameters, maximize the stabilization against oxidation, light, temperature
and color degradation with the use of phenolic acids as co-pigments, determine the best Fe to Ni dopant
ratio and lastly, to determine the current and voltage corresponding to the highest light conversion
efficiency obtained.
33

9th
DOST - PCIEERD
OPTIMIZATION OF DSSC WITH METAL DOPED TIO2 PHOTOANODE
AND VARIOUS ELECTRODE
Kendrick Chiu1
, Tiffany Joy Ong Hian Huy1
, Franco Sosuan1
*
1
In an era racing towards more sustainable energy solutions, dye sensitized solar cells (DSSC)
have been a promising category in solar due to its low fabrication cost. The hindrance to its application
is its low energy output. Individual researches have shown that cationic dopants have varying but
promising effects on photoconductivity while newer carbon allotropes have been centers of much
research attention for their conductive properties. This study aims to investigate the combinatorial
effect of type of dopant on the titania photoanode and type of carbon electrode on the power output of
the cell. The dopants under investigation are transition metals of silver, copper, and zinc and the
counter-electrode substrates are multi-walled carbon nanotubes (MWCNT), graphene and mixture of
both. The solgel route will be utilized for the synthesis and doping method of titania photoanode.
Quality of photoanode will be verified using SEM-EDX. N3 dye sensitizer and iodide/triodide redox
system in acetonitrile solution will be held constant among all cells produced. A full factorial method
totaling nine (9) cells will be used to investigate the varied combinations. Using potentiometric devices
and solarimeter, the fill factor and power efficiency of each cell will be determined. These dependent
parameters will be sued to determine the optimal combination as well as the more significant factor
influencing power output. A significant increase in power parameter implies favorable improvements
to optimizing the power output of DSSC in the future.
34

9th
DOST - PCIEERD
ISOLATION AND IDENTIFICATION OF CHLORELLA-LIKE SPECIES FOR
POTENTIAL APPLICATION IN BIOFUEL PRODUCTION
Nur Faiqah Ismail1
, Mardiana Mohd Ashaari1
, Shaharah Mohd Idris3
,
Normawaty Mohammad Noor2
, and Khairul Bariyyah Abd Halim1*
1
Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, 25200 Kuantan,
Pahang.
2
Department of Marine, Kulliyyah of Science, International Islamic University Malaysia, 25200 Kuantan, Pahang.
3
Fisheries Research Institute, Tanjung Demong, 22200 Besut, Terengganu.
*E-mail: kbariyyah@iium.edu.my
In recent years, there has been an increasing interest in the study of microalgae for biofuel
application. Previous studies have reported that a microalga is able to accumulate lipid, the biodiesel
feedstock, thus hold a great potential as renewable energy source. This project aimed to isolate and
study Chlorella-like algae as new biodiesel source.Water sample contained microalgae was obtained
from Fishery Research Institute, Tanjung Demong, Besut, Terengganu and freshwater lake, Kluang,
Johor. Micractinium reisseri RAIW01 and Micractinium reisseri YSL006 were successfully isolated
based on microscopic examination and LSU rDNA (D1-D2) sequence analysis. Studies on dry weight
and lipid content of Micractinium reisseri RAIW01 showed this strain produce 0.117 ± 0.004 g dwt/
L and 0.028 ± 0.001 g/L respectively and major fatty acid composition was linolenic acid
(37%).Biomass productivity, lipid cell content, and overall lipid productivity are some of the key
parameters that determine the economic feasibility of algal oil for biodiesel production. Based on
result obtained for Micractinium reisseri RAIW01, it indicated that this strain is suitable candidate for
biodiesel production as it able to produce high biomass and consists common fatty acid in biodiesel.
Keywords: GPAT, triacylglycerol, Micractinium
35

Asian Federation of Biotechology Regional Symposium 2017

Asian Federation of Biotechology Regional Symposium 2017

Empfohlen

Empfohlen

Weitere ähnliche Inhalte

Andere mochten auch

Andere mochten auch (14)

Ähnlich wie Asian Federation of Biotechology Regional Symposium 2017

Ähnlich wie Asian Federation of Biotechology Regional Symposium 2017 (20)

Mehr von Karl Obispo

Mehr von Karl Obispo (20)

Kürzlich hochgeladen

Kürzlich hochgeladen (20)

Asian Federation of Biotechology Regional Symposium 2017