3. PRINCIPLES OF LAMINAR AIR FLOW
Laminar Air Flow provides a work area with
Aseptic/sterile conditions for the tissue culture
Laminar Air Flow has continuous displacement
of air (it provides streamline flow of air) that
passes through HEPA (High Efficiency Particulate
Air) filter that removes the particulates from the
4. WHAT IS LAMINAR AIR FLOW?
A laminar air flow workstation is a closed
cabinet fitted with HEPA filtered air flow system.
Here, laminar means unidirectional
constant flow of air with almost no or
5. The purpose of using such workstations in
laboratory is to create particle and bacteria
free working environment to carry out
6. As these units discharge air towards user,
they provide no personal protection but
product protection from room contaminants.
LAF is short form of laminar air flow.
7. LAMINAR AIR FLOW APPLICATIONS :
TYPES OF LAMINAR AIR FLOW
1.Vertical Laminar Air Flow
2.Horizontal Laminar Air Flow
Laminar air flow systems are used in various applications such as
life science research, microbiology, IVF, plant tissue and cell
culture and pharmaceutical and many more.
8. 1.Vertical Laminar Air flow
It is the most used type of laminar air
In vertical flow cabinet, room air is
entered in working area through HEPA
filter fitted on top of the cabinet.
Thus, air flows downward (vertically)
towards work surface and leaves the
cabinet sweeping out particles and
10. 2. Horizontal Laminar Air Flow
In horizontal air flow cabinet, room air is
entered in working area through HEPA filter
fitted on the back side of the cabinet.
Filtered air flows horizontally towards the
user creating sterile working environment.
13. PARTS OF LAMINAR AIR FLOW CHAMBER
Blower assembly draws room air through
pre filter and throws towards HEPA filter
14. Pre Filter:
It is primary air filter which filters
room air initially and forward towards
HEPA filter. Usually, pre filter keeps
out particles 5 microns or higher.
15. HEPA Filter
This is final filter. Air filtered through pre
filter is forwarded towards HEPA filter. HEPA
filter offers 99.99% efficiency for 0.3 micron
17. Work bench :
It is made of stainless steel. Samples /
products are put on it for work.
18. Sash :
Sash is sliding front door. User can slide it
manually and adjust the opening height as
Light is fitted to provide proper
illumination under the working area
Service fixtures :
Main chamber is fitted with electrical
socket, air and gas cock.
20. Germicidal UV lamp
UV lamp is an added option which is used to
sterilize working area prior use. It is advised no
contact to made in chamber during this time.
21. Air Filtration :
The efficiency of a Laminar Air Flow workstation
depends upon the quality of pre filters and HEPA
In LAF, the Air filtration system is created to
work efficiently even in critical environment.
Work Area :
The working area is carefully designed for
comfortable working and easy cleaning.
22. PRECAUTIONS :
Do not the switch on the UV lamp during the
Do not enter head in the laminar work station
or laminar hood
Movement of the analyst should
be slow or controlled during work to allow
Unidirectional air flow
Sanitize hands by sanitizing agent to reduce the
(1) What is full form name of HEPA ?
Ans : High Efficiency Particulate Air
(2) How many types of Laminar air flow ?
Ans : TWO / 1. Vertical LAF & 2. Horizontal LAF
(3) Laminar air flow systems are used in various applications
such as …………..
Ans :life science research, microbiology, IVF, plant tissue and
cell culture and many more.