1. Name : JIGNESH.M.PRAJAPATI
Roll No: 05
LAMINAR AIR FLOW
M.Sc. Sem.- 2
GUIDED BY : Dr. V. A. PATEL SIR

2. LAMINAR AIR FLOW

3. PRINCIPLES OF LAMINAR AIR FLOW
Laminar Air Flow provides a work area with
Aseptic/sterile conditions for the tissue culture
Laminar Air Flow has continuous displacement
of air (it provides streamline flow of air) that
passes through HEPA (High Efficiency Particulate
Air) filter that removes the particulates from the
air

4. WHAT IS LAMINAR AIR FLOW?
A laminar air flow workstation is a closed
cabinet fitted with HEPA filtered air flow system.
Here, laminar means unidirectional
constant flow of air with almost no or
minimal turbulence.

5. The purpose of using such workstations in
laboratory is to create particle and bacteria
free working environment to carry out
specialized work.

6.  As these units discharge air towards user,
they provide no personal protection but
product protection from room contaminants.
 LAF is short form of laminar air flow.

7. LAMINAR AIR FLOW APPLICATIONS :
TYPES OF LAMINAR AIR FLOW
1.Vertical Laminar Air Flow
2.Horizontal Laminar Air Flow
Laminar air flow systems are used in various applications such as
life science research, microbiology, IVF, plant tissue and cell
culture and pharmaceutical and many more.

8. 1.Vertical Laminar Air flow
It is the most used type of laminar air
flow.
In vertical flow cabinet, room air is
entered in working area through HEPA
filter fitted on top of the cabinet.
Thus, air flows downward (vertically)
towards work surface and leaves the
cabinet sweeping out particles and
bacteria

10. 2. Horizontal Laminar Air Flow
In horizontal air flow cabinet, room air is
entered in working area through HEPA filter
fitted on the back side of the cabinet.
Filtered air flows horizontally towards the
user creating sterile working environment.

13. PARTS OF LAMINAR AIR FLOW CHAMBER
Blower :
Blower assembly draws room air through
pre filter and throws towards HEPA filter

14. Pre Filter:
It is primary air filter which filters
room air initially and forward towards
HEPA filter. Usually, pre filter keeps
out particles 5 microns or higher.

15. HEPA Filter
This is final filter. Air filtered through pre
filter is forwarded towards HEPA filter. HEPA
filter offers 99.99% efficiency for 0.3 micron
particulates.

16. Pressure gauge
Magnehelic gauge is used to monitor
differential pressure across HEPA filter with
respect to ambient

17. Work bench :
It is made of stainless steel. Samples /
products are put on it for work.

18. Sash :
Sash is sliding front door. User can slide it
manually and adjust the opening height as
needed.
Light :
Light is fitted to provide proper
illumination under the working area
Service fixtures :
Main chamber is fitted with electrical
socket, air and gas cock.

20. Germicidal UV lamp
UV lamp is an added option which is used to
sterilize working area prior use. It is advised no
contact to made in chamber during this time.

21. Air Filtration :
The efficiency of a Laminar Air Flow workstation
depends upon the quality of pre filters and HEPA
filters.
In LAF, the Air filtration system is created to
work efficiently even in critical environment.
Work Area :
The working area is carefully designed for
comfortable working and easy cleaning.

22. PRECAUTIONS :
Do not the switch on the UV lamp during the
working period
Do not enter head in the laminar work station
or laminar hood
Movement of the analyst should
be slow or controlled during work to allow
Unidirectional air flow
 Sanitize hands by sanitizing agent to reduce the
microbial load.

23. Avoid talking,sneezing ,coughing during
microbial work. if any of occurs stop working
and follow the cleaning activity and continue
the work

24. QUESTION
(1) What is full form name of HEPA ?
Ans : High Efficiency Particulate Air
(2) How many types of Laminar air flow ?
Ans : TWO / 1. Vertical LAF & 2. Horizontal LAF
(3) Laminar air flow systems are used in various applications
such as …………..
Ans :life science research, microbiology, IVF, plant tissue and
cell culture and many more.

25. (1) https://www.stericox.com/cleanroom-
equipment/laminar-air-flow.html
(2) Principles and Techniques of
Biochemistry and
Molecular Biology
Seventh edition
Edited by
KEITH WILSON AND JOHN WALKER