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Internship presentation.pptx

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Internship presentation.pptx

  1. 1. Welcome to my Presentation on Induced Breeding technique of Common Carp( Cyprinous carpio) Presented by, Jannatul Ferdush Roll No: 2006 Reg. No:2016-814-260 Session: 2016-2017 Department of Fisheries University of Dhaka 1
  2. 2. What is induced breeding?  Induced breeding is a technique by which the economically important fish (which generally do not breed in captive condition) are bred through artificial stimulation.  Induced breeding is a technique whereby ripe fish breeders are stimulated by pituitary hormone or any other synthetic hormone introduction to breed in captive condition. The stimulation promotes timely release of sperms and eggs. 2
  3. 3. History of induced breeding  The technique of induced breeding was first evolved in Argentina after producing pituitary extract by Houssay 1930 where viviparous fish was injected with the hormone make premature birth.  In the year of 1934, Brazilians were succeeded in induced breeding pituitary extract. This technique was also followed in America (Merlin & Hubs) and in Russia (Gerebilisky).  In Bangladesh, successful induced breeding was first perfumed by Ali (1967) in carps through hypophysation and later on have been standardized ( Islam and Chowdhury, 1976; Ahmed,1983). 3
  4. 4. Objective of the study  The objective of the internship mainly divided into two parts, the parts are given below:  A. Academic purpose: To fulfill the course requirement for completion of the degree of Honors at University of Dhaka.  B. Practical Purpose: a) To know the basic concept of scientific method of Induced Breeding. b) To find out the potentialities and a problems related to Induced Breeding. c) To analyze farmers acceptance towards the Induced Breeding of Carp. 4
  5. 5. Common carp  Common carp (Cyprinus carpio) belongs to the order Cypriniformes and the family Cyprinidae, which is considered the largest family of freshwater fish. It generally inhabits freshwater environments, especially ponds, lakes and rivers, and also rarely inhabits brackish-water environments. It is widely distributed in almost all countries of the world but is very popular in Asia and some European countries. Because of its high popularity, its distribution has been widely extended by human introduction.  Common carp is a very popular fish item in different country along with Bangladeshi consumer. Generally common carp breeds in natural water bodies. But artificial breeding in commercial community and farm level is too much important for the successful expansion of aquaculture and farmers economic condition. 5
  6. 6.  Carp are mainly bottom dwellers but search for food in the middle and upper layers of the water body. The ecological spectrum of carp is broad. Best growth is obtained when water temperature ranges between 23 °C and 30 °C. The fish can survive cold winter periods. Salinity up to about 5‰ is tolerated. The optimal pH range is 6.5-9.0.  Carp are omnivorous, with a high tendency towards the consumption of animal food, such as water insects, larvae of insects, worms, molluscs, and zooplankton. Zooplankton consumption is dominant in fish ponds where the stocking density is high. Additionally, the carp consumes the stalks, leaves and seeds of aquatic and terrestrial plants, decayed aquatic plants, etc. The daily growth of carp can be 2 to 4 percent of body weigh. 6 Habitat and Biology
  7. 7. Internship location and duration  The experiment was conducted at a private hatchery named as “Gobindapur Fish Hatchery” Hajir Bazar, Feni. From February 23, 2021 to March 8, 2021. The study area is shown in figure below. 7 Figure-1: Interior part of the hatchery
  8. 8. Infrastructure of the hatchery  Brood-fish ponds They have total 16 numbers of ponds (figure-2) where 10 of them are their own and 6 are from lease. In those pond they rear brood fishes. They collect brood fish from other fishermen .they mainly practiced mixed or poly culture of different types of carps like Common carp, Bighead carp, silver carp, Thai pangus at a season and an another season they culture Major carps ( Rui, Catla, Mrigal ). Figure-2: Brood-fish pond 8
  9. 9. Infrastructure of hatchery (continue………….)  Conditioning tank: Tanks are made of concrete. There are two types of conditioning tank (figure-3) in the hatchery on the basis of fish character. For fishes, enable to jump are kept in tank, which are round in shape are depth are higher and covered with a net on the upper surface. And the fishes, unable to jump are kept in a tanks, which are oval in shape, low depth are do not covered with net. Figure-3: conditioning tank 9
  10. 10.  Incubation tank: There are 12 incubation tank (figure-4) and all of them are made of concrete.The incubation or hatching tanks are with specifications of total volume 1400 liter and net egg incubation with an inner chamber (covered with nylon bolting cloth to filter the excess water to the central drain pipe), water inlet through a duck-mouth pipes fitted on the bottom of the tanks provide circulation of water 24 hours. The tanks has drainage outlets at the center of the tank. Infrastructure of hatchery (continue………….) Figure-4: Incubation tank 10
  11. 11. Infrastructure of hatchery (continue………….)  Fry rearing tank There are 8 fry rearing tank (figure-5) in the hatchery. All of them are made of concrete and oval shape. Water supply in shower system and a cylinder shape pipe 12 to 15cm diameter with small circular pores covered by net is placed inside of the tank to maintain water level. Figure-5: Fry rearing Tank 11
  12. 12. Infrastructure of hatchery (continue………….)  Water supply tank (overhead tank)  One concrete overhead water (figure-6) storage tank of capacity 1500 liter is installed on the roof top of the hatchery unit to supply water for hatchery operation. Figure-6: Overhead tank 12
  13. 13. Induced breeding process  Collection of brood fish Brood fishes are collected (figure-7) from two different brood fish ponds. Only mature brood fishes are collected from ponds and immature fishes are returns into the ponds again for maturation. After arriving the hatchery, male and female fishes are separated. There are 4 male brood fish and 12 female fish. Male and female brood fishes are kept on separated tanks (ante ponds) before hypophysation. Figure-7: Collection of brood fish 13
  14. 14. Induced breeding process (continue……) Male Female 1. Small in size 1. Relatively large in size. 2. Abdomen normal, not bulky like female 2. Abdomen bulging, elastic and soft. 3. Pectoral fins are rough 3. Pectoral find are slimy. 4. Slightly protruding reddish vent seemed best criteria for male. 4. Small amount of eggs from the ovary with a small pressure were observed for maturity.  Selection of brood-fish: There have some external morphological features, by observing them one can easily distinguish mature fish from the immature one (table-1) 14
  15. 15. Induced breeding process (continue……)  Conditioning of Brood fish  Preparation of hormone Locally available Compound S-GnRHa (ovupin) and Ovulin (figure-8) were used as inducing agent which was collected from market in preserved condition in airtight vials. Each vial contain (Domperidone 100mg + S-GnRHa 0.2mg). It is a peptide supplement that is used to compress the spawning season, coordinate the spawning time and increase the milt production in males. 15 Figure-8: Ovupin and Ovuline
  16. 16. Induced breeding process(continue……)  Mode of Action  The gonadotropin influence maturation of the gonad through gonadal steroid hormones. Contains an analogue of salmon S-GnRHa and a brain neurotransmitter (dopamine) inhibitor. The S-GnRHa elicits the release of stored gonadotropins from the pituitary. The dopamine inhibitor serves to remove other inhibition of S- GnRHa release.  The outcome of using S-GnRHa is a burst of maturational hormone from pituitary that induces final maturation of the gametes via gonadal steroid hormone. These steroid hormones are essential for final gametes maturation. 16
  17. 17. Induced Breeding Process( Continue……)  Administration of hormone Dissolve in 10ml saline water as suspension before injection. It is used at 0.5 ml/kg body weight in female and half dose in male fish. Injection of compound S-GnRHa delivered to the peritoneal (Abdominal Cavity) using standard needle and syringe (figure-9). Brood fish was caught carefully by net and covered by soft cloth.  Rest interval after hormone administration For 8 to 10 hours before stripping. Figure-9: administration of hormone 17
  18. 18. Induced breeding process (continue….)  Collection of egg and milt Eggs and milt were collected from the ovulated females and males by stripping (figure-10) the abdomen of the fishes with a gentle pressure. Eggs were collected first into a dish. Then males were stripped over the same dish. A towel was used to cover the eye of the fishes and it also help to hold fishes during stripping. Figure-10: Collection of egg and milt 18
  19. 19. Induced breeding process(continue….)  Mixing of egg and milt After collecting egg and milt a soft feather was used to mix them properly. Rate of fertilization depends on the way of mixing. So, mixing (figure-11) is very important step. A mixture of soil and water are added with egg and milt composition. It helps to reduce the adhesiveness or stickiness of the egg and milk mixture. When the stickiness was completely disappear, mixture was washed properly by using clean water with the help of a net. Figure-11: Mixing of egg and milt 19
  20. 20. Induced breeding process(continue…..)  Observation of hatchling rate: The fertilized eggs were placed in funnel-type incubators for hatching. A continuous water Flow through the inlets of the incubators was maintained. Hatching started after 42-48 hours of fertilization. When hatching was completed, the hatchlings were collected in a pot (dish) and counted by visual observation using magnifying glass and recorded. Then hatching rate was determined. Percentage hatchability was determined by direct counting of the number of hatchlings of two days old and estimated as follows: Hatchability = No. of hatched eggs No. of total fertilized eggs × 100 20
  21. 21. Induced breeding process(continue……)  Transfer into fry rearing tank When hatchling were turned into fry stage they are transfer into fry rearing tank. Tanks are oval shape and made of concrete. Water supply 24 hours with a shower which enhance the production of oxygen. Fry are rearing in those tank for 4 to 5 days until they reach marketable size.  Feeding of fry After hatching the fry do not take any external food within 70-72 hours. At this time they use the yolk to survive. After 72 hours the fry were feed with boiled egg yolk grounded in a homogenizer. Feeding was done 4 times a day. 21
  22. 22. Induced breeding process(continue……)  Collection of fry for selling: A nylon net was used for collecting fish fry which are about 25mm (marketable size). 4 people hold the 4 side of the net and gently collecting the fry. Dead fry which are floating on the net are discarded. Only live and good fries are collected for selling. Then collected fries are weighted.200 gm. for each packet containing 6 liter water.  Packaging of fry: For packaging they used LED plastic packet. At first all of the packet are fill up with 6 Liter water (figure- 20) and then 200 gm. fries are added to the packet. Then for respiration of fries added oxygen from an oxygen cylinder. This helps fries for their survival of about 15 to 16 hours. 22
  23. 23. Conclusion  In the aquaculture sector, induced breeding has opened the door of a new era throughout the world. From the investigational study it was observed that induced breeding technique contribute to a remarkable part of fish seed production as well as to the aquaculture development in Bangladesh.  This study also indicates the necessity of seed production by this artificial breeding process. To get the better production and high quality fish seed, the proper doses of hormone injection and use of quality brood fishes should be maintained. Though the seed quality is not so good, however, the quantity of fish seed is increasing day by day. 23
  24. 24. Recommendation From the field work followings recommendation would be offered:  The good brood fish should be selected for breeding.  Different brood fish strains should be collected from various sources of origin.  Special attention should give to improve the quality of seed, in this regard, live brood need to be established.  The government in collaboration with private sectors should take necessary steps to established laboratories to conduct research in this sector.  Special attention should be given in hormonal dose calculation. 24
  25. 25. Thanks for patience hearing 25

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