In this power point overview of micropropagation and its classification has been discussed then Steps involved in the micropropagation. And also its contribution towards reforestation.
2. Microprogation
• Budding shoots are culture inside test tubes & nourished on an agar
gel with growth regulators to coax them into multiplying secondary
shoots.
• Then it transplanted into soil, these shoots eventually root and
become whole, free-living plantlets that can be put in the field.
8. Reforestation by
micropropagation
• Forest trees are renewable source of food, fuel wood, timber and
other valuable non-timber products.
• The ever increasing human & livestock populations demand for
plant products; resulting in over exploitation of forest trees.
• There is an urgent need for conservations of germplasm and also
for propagation of a sustainable utilization of forest trees.
• Micropropagation of tree species offers a rapid means of producing
clonal planting stock for afforestation, woody biomass production
& conservation of rare germplasm.
• Therefore millions of plant species can be maintained inside
culture vials in a small room and this is the suitable alternative to
conventional methods of vegetative propagation of plants.
9. Factors affecting micropropagation
• Genotype of the plant.
• Physiological status of the explants.
• Culture media.
• Culture Environment
-Light, temperature and composition of gas phase
10. Advantage
• Able to produce many plants that are clone of
each other.
• Can able to produce disease free plants.
• It is the only viable method of regenerating
genetically modified cells or cells after
protoplast fusion.
• Greater number of plants can be produced per
square meter and the propagules can be stored
longer in a smaller area.
11. Disadvantage
• High chances of contamination
• Infected plant sample can produce infected
progeny
• Not all plant can be successfully
micropropagated.
• Some plants are very difficult to disinfect
fungal organism
• Genetic variation will be low
12. Application
• High rate of plant propagation
• Production of disease-free plants
• Production of seeds in some crops
• Automated micropropagation
