Gabriela Mattos received a BSc in Biochemistry and Molecular Biology from Dalhousie University in 2016, where she conducted honors thesis research on the regulatory role of deubiquitinating enzymes in apolipoprotein B-100 synthesis and degradation under the supervision of Dr. Roger McLeod. She has skills in laboratory techniques and software programs, and is fluent in English, Portuguese, and French. She has experience as a research assistant and has presented her research at conferences. Currently, she works as a banquet server and is seeking opportunities in research.

1. Gabriela Mattos
Curriculum Vitae
PERSONAL DETAILS
Birth May 17, 1994
Address 5808 Tayside Cres., Mississauga, ON
Phone (647) 971-5222
Mail gabrielakmattos@gmail.com
EDUCATION
BSc. Hons. Biochemistry & Molecular Biology, Minor in Microbiology &
Immunology 2012-2016
Dalhousie University
Honours Thesis Research - Supervisor: Dr. Roger McLeod
Researched the regulatory role of deubiquitinating enzymes on apolipoprotein B-100 synthesis
and degradation. Methodology includes cell culture of the human hepatoma cell line, HepG2,
western blot analysis, and pulse chase analysis following cell treatment with proteasome and
broad spectrum deubiquinating enzyme inhibitors.
High School 2008-2012
St. Aloysius Gonzaga S.S
Graduated with honours after partaking in high level science classes including biology,
chemistry, physics, as well as calculus.
SKILLS
Laboratory Knowledgeable in concepts including metabolism, chemistry, cell
biology, signal transduction, and genetics.
Able to work independently in the lab.
Reliable with lab equipment and performing tasks efficiently and
correctly
Languages English (mother tongue)
Portuguese (fluent)
French (fluent)
Software Microsoft Office, Adobe Photoshop, Adobe Illustrator, ImageJ,
Prism.
CERTIFICATIONS
Radiation Safety Training June 16, 2015
Dalhousie University, Office of Environmental Health and Safety
Expires: June 16, 2020
.
Transportation of Dangerous Goods Training-Receiving Class 7 June 16, 2015
Dalhousie University, Office of Environmental Health and Safety
Expires: June 16, 2020

2. .
Biosafety Training Aug. 31, 2015
Dalhousie University, Office of Environmental Health and Safety
CONFERENCE ABSTRACTS
The role of deubiquitinases in hepatic apoB metabolism - DeClercq V., Lake
E., Mattos G., Fisher E. A., McLeod R. S. 2015
Presented at the Canadian Lipoprotein Conference in Toronto on October 16-18, 2015
Apolipoprotein (apo) B is essential for the assembly and secretion of triglyceride-rich VLDL
particles from the liver. Failure to assemble into secretion-competent VLDL can initiate
degradation of apoB by the ubiquitin proteasome system. It is known that ubiquitin is
removed and recycled by de-ubiquitinating enzymes (DUBs) before the degradation of the
client proteins, but it has also been suggested that DUBs may also reverse ubiquitination
early in the targeting pathway and perhaps rescue proteins from degradation. The objective
of this study was to determine if deubiquitinating enzymes (DUBs) are involved in the
stability of ubiquitinated apoB. HepG2 cells were incubated with PR619, a broad-spectrum
inhibitor of DUBs, with or without the proteasome inhibitor MG132. PR619 treatment
with MG132 reduced the accumulation of total and polyubiquitinated apoB compared
to proteasome inhibition alone, suggesting that DUBs may play a role in cellular apoB
transport. Polyubiquitinated apoB was purified using immunoprecipitation and Tandem
Ubiquitin Binding Entities (TUBEs). An enzymatic array was used to screen individual
DUBs for their ability to cleave ubiquitin from the purified apoB. One of the DUBs, Usp15,
was identified as capable of liberating ubiquitin from polyubiquitinated apoB. However,
siRNA-mediated knockdown of Usp15 did not alter the stability or secretion of either apoB
or apoAI from HepG2. Furthermore, immunofluorescent microscopy showed that apoB
and Usp15 had minimal colocalization. In conclusion, inhibition of DUBs in HepG2 cells
reduces ubiquitinated apoB and total apoB100 accumulation. However the specific DUB or
combination of DUBs responsible for these effects remains to be determined.
.
VOLUNTEER EXPERIENCE
Research Assistant 2015
Dalhousie University
Volunteered in the Dr. Roger McLeod lab. I assisted post-doctoral fellows in their research
on apolipoprotein B-100. Through this experience, I was able to learn techniques including
cell culture, western blot analysis, and pulse chase analysis.
Research Assistant 2014
Ryerson University
Volunteered in the Dr. Martina Hausner lab. I assisted a Ph.D candidate in their research
on plasmid transfer between bioflim bacteria. In this lab, I learned techniques including,
bacterial cell culture and bacterial screening.
WORK EXPERIENCE
Banquet Server 2015-present
Atlantica Hotel, Part-time

3. Serve dinner and drinks to guests attending events at the Atlantica Hotel in Halifax, NS.
Events include weddings, business meetings, conferences, and holiday parties.
Halfway House Attendant 2014
Mississauga Golf and Country Club., Part-time
Worked independently and professionally to prepare food and drinks for Mississauga Golf
and Country Club members.
REFERENCES
Available upon request