![SITE-SPECIFIC 11 C -LABELING OF PEPTIDES USING [ 11 C ]METHYLATING AGENTS By Erin Saucke-Lacelle, Esther Schirrmacher, Dean Jolly, Ralf Schirrmacher](https://image.slidesharecdn.com/cfakepathpresentation-dc-091028234010-phpapp02/85/c11-labeling-of-peptides-in-a-single-step-1-320.jpg)
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C-11 Labeling of Peptides in a Single Step
- 1. SITE-SPECIFIC 11 C -LABELING OF PEPTIDES USING [ 11 C ]METHYLATING AGENTS By Erin Saucke-Lacelle, Esther Schirrmacher, Dean Jolly, Ralf Schirrmacher
- 2. OBJECTIVE site-specific labeling of unprotected peptides using 11 C methylating agents in one-step
- 5. Trp-Tyr-Phe-Phe-Lys-Gly 5 side chains 3 possibilities HPLC conditions: Agilent 1200 series multiwavelength UV, Raytest radioactivity detector, LiChrosorb column 250 x 10mm, flow 2.0 ml/min, ACCN (0.05%TFA)/H2O (0.05% TFA), gradient from 100% H2O to 0% within 40 min
- 6. Trp-Tyr-Trp-Ser-Arg-Cys-Lys-Trp-Thr-Gly 9 side chains 7 possibilities HPLC conditions: Agilent 1200 series multiwavelength UV, Raytest radioactivity detector, Chromolith column 100 x 4.6mm, flow 1.4 ml/min, H2O(0.05%TFA)/ ACCN(0.05% TFA), gradient from 100% H2O to 0% within 40 min
Hinweis der Redaktion
- *** write?: 11C-MeI *they say no mo than 5 words……. *7 words wide, 7 lines tall *white words on dark (blue, blk) bkgrnd
- * Is it possible to align all of the titles from all slides?
- Intro: just 1 ex of 11C-labeled peptide that exists?? Not as simple as our method…
- 5 (6?)side chains *remove C’s, just leave Os, S & Ns……
- X side chains, X possibilities, correct? *remove C’s, just leave Os, S & Ns……
- {total synthesis time EOB 25 min + work-up (5-7 min in DCM, 25-30 min in AcCN with HPLC)}