This document provides an overview of plant tissue culture in Bangladesh. It discusses the history of plant tissue culture, types of plant tissue culture, current research being done in Bangladesh, and constraints and recommendations. Research on plant tissue culture in Bangladesh began in the 1970s at the University of Dhaka with jute tissue culture. Since then, research laboratories have been established at various universities and research organizations. Current research includes tissue culture of crops like banana, jackfruit, pineapple, tobacco, and potato. Constraints to further development include a lack of infrastructure, funding, and skilled personnel. Recommendations include establishing a gene bank, improving laboratory facilities, developing biotechnology policies and funding, and fostering international collaboration.

1. Present Status of
Plant Tissue
Culture in
Bangladesh
Presented by
Dipta Bardhan
Registration no.: 2012431040
Course no.: Geb 300

2. Will be Discussed
Plant Tissue Culture
History
Types of Plant tissue culture
Plant tissue culture in Bangladesh
Importance of plant tissue culture in Bangladesh
Plant Tissue Culture Research in Bangladesh
Constrains
Recommendation
Reference

3. Plant Tissue Culture
Plant tissue culture is the science of growing plant cells, tissues or
organs isolated from the Mother plant, on artificial media in vitro under
controlled conditions

4. History
HABERLANDT is considered to be the father of plant tissue culture who
conceived the concept of totipotency & cell culture in 1902.
Totipotency is the ability of a single cell to divide and produce all types
of differentiated cells in an organism.
He was the first to consider culturing cells aseptically in a nutrient
solution.
However, he was unsuccessful in his culture as he failed to recognize
that cell differentiation requires plant growth regulators

5. Types of Plant tissue
culture
Plant Tissue Culture
Callus Culture
Cell Culture
Protoplast Culture
Organ Culture Embryo Culture
Seed Culture
Bud Culture
Meristem Culture

6. Plant tissue culture in
Bangladesh
The program on plant biotechnology in Bangladesh was initiated in late
1970s in the Department of Botany, University of Dhaka with tissue
culture of jute.
Thereafter within a span of 10-12 years tissue culture research
laboratories had been developed in different universities, R&D
organizations, private entrepreneurs. A few NGOs are working on plant
tissue culture.
BRAC & PROSHIKA have already marketed tissue cultured plantlets such
as potato, banana and ornamental plants in Bangladesh and
neighboring countries.

7. Importance of Plant Tissue
Culture in Bangladesh
•Production of improved crop varieties
•Production of disease-free plants (virus)
•Genetic transformation
•Production of secondary metabolites
•Production of varieties tolerant to salinity, drought and heat stresses

8. Plant Tissue Culture
Research in Bangladesh
Research on Plant
tissue Culture
Research done by
Institute/personnel
Image
In vitro
regeneration of
banana plantlet
Md. Al-amin, M. R.
Karim, M. R. Amin, S.
Rahman And A. N. M.
Mamun,
BARI
In vitro
regeneration of
jackfruit
Biotechnology Division,
BARI
In vitro
regeneration of
pineapple
Biotechnology division,
BARI

9. Plant Tissue Culture
Research in Bangladesh
Research on Plant tissue
Culture
Research done by
Institute/personnel
Image
In vitro regeneration of
gladiolus
Biotechnology division,
BARI
Plantlet development
from the pollen
S Khatun and K A Billah,
BARI
In vitro culture of
orchids
M. M. Khatun, H.
Khatun, D. Khanam And
Md. Al-amin, BARI

10. Plant Tissue Culture
Research in Bangladesh
Research on Plant tissue
Culture
Research done by
Institute/personnel
Image
In vitro propagation of
tobacco plantlet
M. A. Rahman, M.A.
Alam, M.R. Hossain A.
Hos, Sain And R. Afroz,
BARI
In vitro regeneration of
anthodium andreanum
cv. Nitta
S.A. Islam, M.M.R.
Dewan, M.H.R. Mukul
M.A. Hossen And F.
Khatun, DEBTEC
(Development Of
Biotechnology &
Environmental
Conservation Centre)
Laboratory, Dhaka.

11. Plant Tissue Culture
Research in Bangladesh
Research on Plant tissue
Culture
Research done by
Institute/personnel
Image
Plant regeneration obtain
from the cotyledon of the
c.olitorious on ms agar
solidified medium by IAA .5
mg/l and BAP 3.0mg/l
Kazi md kamrul
huda in BSRI in june
2006 dept of
genetics and plant
breeding,SAU
Significant variations were
observed among the
genotypes and media
composition for shoot
regeneration. Among the
genotypes, BARI Sarisha-8
showed the best
performance for shoot
regeneration followed by
BARJ Sarisha-l1
M. A. Sayem,
Maniruzzaman, S. S.
Siddique And M. Al-
Amin,BARI

12. Plant Tissue Culture
Research in Bangladesh
Research on Plant
tissue Culture
Research done by
Institute/personnel
Image
In Vitro Propagation
Of Pointed Gourd
(Trichosanthes Dioica
Roxb.) Through
Encapsulated Shoot
Tips
M. A. Malek, M. A. Bari
Miah, M. Al-amin, D.
Khanam And M.
Khatun,BARI
Haploid plantlet
regeneration through
anther culture in
oilseed brassica
species
M. A. Alam, M. A. Haque,
M. R. Hossain S.C. Sarker
And R. Afroz BARI

13. Plant Tissue Culture
Research in Bangladesh
Research on Plant tissue
Culture
Research done by
Institute/personnel
Image
In vitro Callus Initiation
And Regeneration Of
Potato
A. U. Haque, M. A.
Samad And T. L. SHAPLA,
BARI
Micropropagation Of
Strawberry (Fragaria
ananassa) Through
Runner Culture
M. Ashrafuzzaman, S. M.
Faisal , D. Yadav D.
Khanam An, D F.
RAIHAN, BARI
In vitro regeneration of
brinjal (solanum
melongena )
B. P. Ray, L. Hassan And
K. M. Nasiruddin BARI

14. Constrains
•Bangladesh, identified as one of the world’s biodiversity “hot spot”
•Biotechnological development in Bangladesh is at its primary stage.
•Lack of infrastructure and shortage of funds and skilled manpower are
hindering the progress in biotechnological research.
•Again, it cannot be used due to lack of interest among entrepreneurs.
Entrepreneurs are more interested in the import of technology to get quick
returns. Initiative and investment are lacking.
•Bangladesh needs a clear policy, institutional development and funding for the
application of biotechnology to solve the problems in agriculture, industry,
health and environment.
•Integration of molecular and cellular biology with conventional breeding
technologies can be used for the development of new crop varieties,
improvement of feed and livestock. It can help health and energy sectors.

15. Recommendation
•Establishment of a gene bank.
•Establishment of institutional wise sophisticated laboratory.
•Proper government policy should be required for development of
Biotechnological frame work.
•Collaboration between the national and international research
organization.
•Funding is a major problem in country. Financial support shall be
provisioned towards the activities from - government, private sector,
donor agencies.

16. Reference
http://www.saarc-sec.org/userfiles/NationalStatesof-the-ArtReport.pdf
http://www.bbc.co.uk/staticarchive
http://www.slideshare.net
http://www.intechopen.com/books/recent-advances-in-plant-in-vitro-
culture/plant-tissue-culture-current-status-and-opportunities
http://www.biologydiscussion.com/plants/plant-tissue-culture-
environmental-condition-methods-types-and-application/1359
http://www.biologydiscussion.com/plants/plant-tissue-culture/plant-
tissue-culture-benefit-structure-types-and-techniques/10632

17. Thank You