1. CURRICULUM VITAE
Wen-Hai Chou, PhD
CONTACT INFORMATION
Department of Biological Sciences Office phone: (330) 672-2979
Kent State University Lab phone: (330) 672-2993
41 Cunningham Hall Email: wenhaichou@gmail.com
Kent, OH 44242, USA wchou2@kent.edu
http://www2.kent.edu/profiles/profile_detail.cfm?profileitem=wchou2
Citizenship: USA
EDUCATION
Ph.D., Department of Molecular Medicine, University of Texas Health Science Center, San
Antonio, Texas, May 2000.
Master of Science, Department of Biology, Texas A&M University, College Station, Texas, May
1993.
Bachelor of Science, Department of Entomology, National Chung-Hsing University, Taichung,
Taiwan, May 1987.
RESEARCH AND PROFESSIONAL EXPERIENCE
Kent State University, Kent, Ohio August 2010 - present.
Assistant Professor, Department of Biological Sciences and School of Biomedical Sciences,
College of Arts and Sciences.
Courses: Human Biology, Bioenergetics, Individual Investigation.
Research: The roles of Protein Kinase C and Lipocalin-2 in stroke.
University of California, San Francisco, September 2006 – July 2010.
Assistant Adjunct Professor, Department of Neurology, School of Medicine.
Research: Protein Kinase C in neurological diseases.
University of California, San Francisco, July 2005 – July 2010.
Associate Investigator, Ernest Gallo Clinic & Research Center, School of Medicine.
Research: Regulation of GABAA receptor trafficking by Protein Kinase C.
University of California, San Francisco, August 2001 – August 2005.
Assistant Research Scientist, Ernest Gallo Clinic & Research Center, School of Medicine.
Research: Protein Kinase C in alcoholism and drug addition.
University of Texas Health Science Center, San Antonio, August 2000 – August 2001.
Postdoctoral Fellow, Department of Biochemistry.
Research: Structural studies of the clathrin assembly and disassembly by adaptor proteins
during endocytosis.
University of California, Los Angeles, February 2000 – July 2000.
Postgraduate Researcher, Howard Hughes Medical Institute and Department of Neurology,
School of Medicine.
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2. Wen-Hai Chou, PhD
Research: Drosophila model of the Huntington’s disease.
University of Texas Health Science Center, San Antonio, August 1993 – December 1999.
Research Assistant and Teaching Assistant, Department of Molecular Medicine, School of
Medicine.
Courses: Advanced Molecular Biology and its Laboratory for graduate students.
Research: Molecular and genetic studies of R7 and R8 photoreceptor cell differentiation in
the Drosophila retina.
Texas A&M University, College Station, January 1990 – May 1993.
Research Assistant and Teaching Assistant, Department of Biology, College of Science.
Courses: Introductory Biology Laboratory for undergraduate students.
Research: Purification of farnesoic acid O-methyltransferase, a key enzyme involved in the
biosynthesis of juvenile hormone.
TECHNIQUE SKILLS
Animal models: Microsurgery to induce focal cerebral ischemia (transient and permanent
middle cerebral artery occlusion model) and global cerebral ischemia (hypotension and
bilateral common carotid artery occlusion); Measurement of regional cerebral blood flow
using Laser Doppler Flowmetry; Visualization of cerebrovascular anatomy; Bone marrow
transplantation to exchange hematopoietic composition between mice; Isolation of murine
bone marrow; Collection of mouse blood serum; Genotyping; Mouse breeding and
husbandry; Drosophila culture and genetics; Immunohistochemistry; Immunofluorescence
and confocal microscopy.
Animal behavior tests: Open field, elevated plus maze and light-dark box for locomotor and
anxiety-like behaviors; Barnes and water mazes for spatial memory; Neurological deficit
scores and corner tests for sensorimotor deficits; Rotarod test for motor coordination; Loss
of righting reflex (LORR) to calculate an ED50 for ethanol-induced LORR.
Cell culture models: Primary neuronal cultures; Mammalian cell cultures; Cell culture model of
ischemic stroke (oxygen glucose deprivation, OGD); Transfection; Immunocytochemistry;
Isolation and analysis of neutrophil functions (adhesion, migration, superoxide release,
degranulation).
Molecular, cellular and biochemical methods: Develop and design chemical-genetic analysis
for kinases; Kinase assay; Protein purification using FPLC; Protein Profiling by Microscale
Solution Isoelectrofocusing (MicroSol-IEF, Invitrogen); Antibody purification and labeling;
Design and develop phospho-specific antibody; ATPase assay; Immunoprecipitation; GST-
binding assay, gel overlay assay and yeast two-hybrid analysis for protein-protein
interactions; Western blot; PCR; Agarose gel electrophoresis; ELISA; molecular cloning.
Bioinformatics: Analysis of protein structures using Chimera; Docking of compounds using
AutoDock Vina integrated with Chimera; Drawing and energy minimization of compounds
using Marvin Sketch.
Statistical analysis: One- or two-way ANOVA with post-hoc tests, non-parametric Kruskal-
Wallis test with Dunn’s post test, Student’s t tests, and power analysis.
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3. Wen-Hai Chou, PhD
HONORS AND AWARDS
Elected as a Fellow of the American Heart Association (F.A.H.A) for making major and
productive contributions in cardiovascular basic sciences, 2011.
Certificate of Achievement from University of Texas Health Science Center at San Antonio,
Texas, 2001.
Certificate of Achievement from National Chung-Hsing University, Taichung, Taiwan, 2001.
First-place poster award at the Annual Graduate Student Association Forum, University of Texas
Health Science Center at San Antonio, Texas, 1998
Second-place outstanding research award at the Annual Retreat, Institute of Biotechnology,
University of Texas Health Science Center at San Antonio, Texas, 1998
Travel award for distinguish research from Institute of Biotechnology, University of Texas
Health Science Center at San Antonio, Texas, 1997
PATENTS
Filing of provisional patent “DETECTION OF ISCHEMIC STROKES VIA A PLASMA
BIOMARKER AND TREATMENT THEREOF” on May 14, 2013.
PEER-REVIEWED PUBLICATIONS (IN PREPARATION)
1. Kumar, V., Wang, G., Weng, Y.-C., Kristian, T., Messing, R.O., *Chou, W.-H. (2016) PKCε
regulation of nuclear ATF2 leakage in ischemia-induced neurodegeneration.
(*corresponding author)
2. Wang, G., Weng, Y.-C., *Chou, W.-H. (2016) Inhibition of lipocalin-2 reduces stroke-
reperfusion injury. (*corresponding author)
PEER-REVIEWED PUBLICATIONS
(Complete List of My Published Work and Citation in Google Scholar)
1. Kumar, V., Weng, Y.-C., Geldenhuys, W.J., Wang, D., Han, X., Messing, R.O., *Chou, W.-H.
(2015) Generation and Characterization of ATP Analog-specific Protein Kinas C delta.
Journal of Biological Chemistry. 290(4):1936–51. (*corresponding author) (JBC impact
factor: 4.6) (JBC ranking: 69/291 in the Category of BIOCHEMISTRY &
MOLECULAR BIOLOGY)
2. Wang, G., Weng, Y.-C., Han, X., Whaley, J.D., McCrae, K.R., *Chou, W.-H. (2015)
Lipocalin-2 released in response to cerebral ischemia mediates reperfusion injury in mice.
Journal of Cellular and Molecular Medicine. 19(7):1637-45. (*corresponding author)
(JCMM impact factor: 4.8) (JCMM ranking: 27/124 in the Category of MEDICINE,
RESEARCH & EXPERIMENTAL)
3. Weng, Y.-C., Wang, G., Messing, R.O., *Chou, W.-H. (2015) Identification of lipocalin-2 as a
PKC delta phosphorylation substrate in neutrophils. Journal of Biomedical Science. 22:21
(*corresponding author) (JBMS impact factor: 2.76) (JBMS ranking: 49/124 in the
Category of MEDICINE, RESEARCH & EXPERIMENTAL)
4. *Chou, W.-H., Wang, G., Kumar, V., Weng, Y.-C. (2015) Lipocalin-2 in Stroke. Neuro. 2(1):
38-41 (Invited Review) (*corresponding author)
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4. Wen-Hai Chou, PhD
5. Weng, Y.-C., *Chou, W.-H. (2015) Neutrophil Gelatinase-Associated Lipocalin and Matrix
Metalloproteinase-9 as Potential Biomarkers for Stroke: A Pilot Study. Journal of
Neurology & Neurophysiology. 6(2):278 (*corresponding author)
6. Szular, J., Sehadova, H., Gentile, C., Szabo, G., Chou, W.-H., Britt, S. G., and *Stanewsky, R.
(2012) Rhodopsin 5 and Rhodopsin 6 mediated clock synchronization in Drosophila
melanogaster is independent of retinal Phospholipase C-ß signaling. Journal of
Biological Rhythms 27(1):25-36. (*corresponding author) (Cited by 5 articles) (JBR
impact factor: 3.316) (JBR ranking: 16/85 in the Category of BIOLOGY)
7. Chiang, T., Messing, R.O., and *Chou, W.-H. (2011). Mouse model of middle cerebral artery
occlusion. Journal of Visualized Experiments 48:2761. (*corresponding author) (Cited by
20 articles)
8. Chou, W.-H., Wang, D., McMahon, T., Qi, Z.-H., Song, M., Zhang, C., Shokat, K.M. and
*Messing, R.O. (2010) GABAA Receptor Trafficking is Regulated by PKC epsilon and
the N-Ethylmaleimide-Sensitive Factor. Journal of Neuroscience 30(42):13955-65.
(*corresponding author) (Cited by 27 articles) (JN impact factor: 6.747) (JN ranking:
24/252 in the Category of NEUROSCIENCES)
9. Birkholz, D. A., Chou, W.-H., Phistry, M., *Britt, S. G. (2009) Disruption of photoreceptor
patterning in the Drosophila Scutoid mutant. Fly 3(4):253-62. (*corresponding author)
(Cited by 1 article) (Fly impact factor: 3.325) (FLY ranking: 244/291 in the Category of
BIOCHEMISTRY & MOLECULAR BIOLOGY)
10. Birkholz, D. A., Chou, W.-H., Phistry, M., *Britt, S. G. (2009) rhomboid Mediates
Specification of Blue- and Green-sensitive R8 Photoreceptor Cells in Drosophila.
Journal of Neuroscience 29(9):2666-75. (*corresponding author) (Cited by 2 articles) (JN
impact factor: 6.747) (JN ranking: 24/252 in the Category of NEUROSCIENCES)
11. Chou, W.-H. and *Messing, R. O. (2008) Hypertensive encephalopathy and the blood-brain
barrier: Is deltaPKC a gatekeeper? Journal of Clinical Investigation 118(1):17-20.
(*corresponding author) (Cited by 20 articles) (JCI impact factor: 13.215) (JCI ranking:
5/124 in the Category of MEDICINE, RESEARCH & EXPERIMENTAL)
12. Lesscher H. M. B., McMahon T., Lasek A. W., Chou, W.-H., Connolly, J., Kharazia, V. N.,
*Messing, R. O. (2008) Amygdala PKC epsilon regulates corticotropin-releasing factor
and anxiety-like behavior. Genes, Brain and Behavior 7(3):323-33. (*corresponding
author) (Cited by 26 articles) (GBB impact factor: 3.597) (GBB ranking: 10/49 in the
Category of BEHAVIORAL SCIENCES)
13. Qi Z.-H., Song, M., Wallace, M. J., Wang, D., Newton, P. M., McMahon T., Chou, W.-H.,
Zhang, C., Shokat, K. M., *Messing, R. O. (2007) PKC epsilon regulates GABAA
receptor sensitivity to ethanol and benzodiazepines through phosphorylation of gamma2
subunits. Journal of Biological Chemistry 282(45):33052-63. (*corresponding author)
(Cited by 60 articles) (JBC impact factor: 4.6) (JBC ranking: 69/291 in the Category of
BIOCHEMISTRY & MOLECULAR BIOLOGY)
14. Allen, J. J., Li, M., Brinkworth, C. S., Paulson J. L., Wang, D., Hübner A., Chou, W.-H.,
Davis, R. J., Burlingame, A. L., Messing, R. O., Katayama, C. D., Hedrick, S. M.,
*Shokat, K. M. (2007) A semi-synthetic epitope for kinase substrates. Nature Methods
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5. Wen-Hai Chou, PhD
4(6):511-6. (*corresponding author) (Cited by 122 articles) (NM impact factor: 27.363)
(NM ranking: 1/78 in the Category of BIOCHEMICAL RESEARCH METHODS)
15. Wallace, M. J., Newton, P. M., Oyasu, M., McMahon, T., Chou, W.-H., Connolly, J., and
*Messing, R. O. (2007) Acute functional tolerance to ethanol mediated by Protein Kinase
C epsilon. Neuropsychopharmacology 32(1):127-36. (*corresponding author) (Cited by
39 articles) (NPP impact factor: 7.048) (NPP ranking: 17/252 in the Category of
NEUROSCIENCES)
16. Chou, W.-H. and *Messing, R. O. (2005) Protein kinase C isozymes in stroke. Trends in
Cardiovascular Medicine 15(2):47-51. (*corresponding author) (Invited Review) (Cited
by 58 articles) (TICN impact factor: 2.906) (TICM ranking: 67/125 in the Category of
CARDIAC & CARDIOVASCULAR SYSTEMS)
17. Chou, W.-H., Choi, D.-S., Zhang, H., Mu, D., McMahon, T., Kharazia, V. N., Lowell, C. A.,
Ferriero, D. M. and *Messing, R. O. (2004) Neutrophil protein kinase C delta as a
mediator of stroke-reperfusion injury. Journal of Clinical Investigation 114(1):49-56.
(*corresponding author) (Cited by 114 articles) (JCI impact factor: 13.215) (JCI ranking:
5/124 in the Category of MEDICINE, RESEARCH & EXPERIMENTAL)
18. Knox, B. E., Salcedo, E., Mathiesz, K., Schaefer , J., Chou, W.-H., Chadwell, L. V., Smith,
W. C., Britt, S. G., *Barlow, R. B. (2003) Heterologous Expression of Limulus
Rhodopsin. Journal of Biological Chemistry 278(42):40493-502. (*corresponding author)
(Cited by 18 articles) (JBC impact factor: 4.6) (JBC ranking: 69/291 in the Category of
BIOCHEMISTRY & MOLECULAR BIOLOGY)
19. Salcedo, E., Huber, A., Heinrich, S., Chadwell, L. V., Chou, W.-H., Paulsen, R. and *Britt, S.
G. (1999) Blue- and green-sensitive visual pigments of Drosophila: Ectopic expression
and physiological characterization of the R8 photoreceptor cell-specific Rh5 and Rh6
rhodopsins. Journal of Neuroscience 19(24):10716-26. (*corresponding author) (Cited by
130 articles) (JN impact factor: 6.747) (JN ranking: 24/252 in the Category of
NEUROSCIENCES)
20. Chou, W.-H., Huber, A., Bentrop, J., Schulz, S., Chadwell, L. V., Paulsen, R., and *Britt, S.
G. (1999) Patterning of the R7 and R8 photoreceptor cells: Evidence for induced and
default cell-fate specification. Development 126(4), 607-16. (*corresponding author)
(Cited by 113 articles) (DEV impact factor: 6.462) (DEV ranking: 4/41 in the Category of
DEVELOPMENTAL BIOLOGY)
21. Chang, K.-H.*, Chen, Y.*, Chen, T.-T.*, Chou, W.-H.*, Chen, P.-L., Ma, Y.-Y., Yang-Feng,
T. L., Leng X., Tsai, M.-J., O’Malley, B. W., and **Lee, W.-H. (1997) A thyroid hormone
receptor coactivator negatively regulated by the retinoblastoma protein. Proceedings of
the National Academy of Sciences 94(17), 9040-5. (* Co-First Authors) (**corresponding
author) (Cited by 73 articles) (PNAS impact factor: 9.674) (PNAS ranking: 4/55 in the
Category of MULTIDISCIPLINARY SCIENCES)
22. Chou, W.-H., Hall, K. J., Wilson, D. B., Wideman, C. L., Townson, S. M., Chadwell, L. V.,
and *Britt, S. G. (1996) Identification of a novel Drosophila opsin reveals specific
patterning of the R7 and R8 photoreceptor cells. Neuron 17(6), 1101-15. (*corresponding
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6. Wen-Hai Chou, PhD
author) (Cited by 138 articles) (Neuron impact factor: 15.054) (Neuron ranking: 4/252 in
the Category of NEUROSCIENCES)
GRANT ACTIVITY
Pending Grants
Co-Principal Investigator with Dr. Vijayaraghavan, “Identification of phospho-proteins regulating
sperm function” from NIH R21, 2015-2018 ($410,778; 1st submission on 3/15/2015, percentile
11%).
Principal Investigator, “The role of PKC epsilon and ATF2 in ischemia-induced neurodegeneration”
from NIH R15, 2015-2018 ($425,553; 1st submission on 2/25/2015, impact score 42, percentile
34%).
Principal Investigator, “The role of PKC epsilon and ATF2 in ischemia-induced neurodegeneration”
from American Heart Association Great Rivers Grant-in-Aid Program, 2015-2017 ($154,000; 1st
submission on 1/13/2015; 2nd submission on 7/22/2015).
Co-Principal Investigator with Dr. Clement et al., “Acquisition of an Optical and X-ray small animal
imaging system for Research and Education” from NSF MRI, 2015-2017 ($221,830; 1st
submission in February 2015, not funded; to be resubmitted in January 2016).
Funded Grants
Principal Investigator, “Neutrophil-gelatinase-associated lipocalin in stroke-reperfusion injury” from
American Heart Association Great Rivers Beginning Grant-in-Aid Program, 2013-2015
($132,000; funded).
Co-Principal Investigator with Dr. McCrae at Cleveland Clinic and Dr. Damron at KSU, “Prevention
of reperfusion injury in ischemic stroke: role of High Molecular Weight Kininogen and PKC
delta” from Kent State University – Cleveland Clinic Lerner Research Institute Collaborative
Research Awards, 2013-2015 ($100,000; funded).
Principal Investigator, “PKC signaling in stroke-reperfusion injury” from Farris Family Innovation
Award, 2011-2014 ($24,000; funded).
Principal Investigator (with Drs. Bowling and Wilber at Summa Akron City Hospital, “NGAL and
MMP-9 as early biomarkers for ischemic stroke” from Initiative for Clinical and Translational
Research (ICTR) Pilot Funding, 2012-2013 ($10,000; funded).
Principle Investigator, “PKC isozymes in stroke-reperfusion injury” from Kent State University
startup fund, 2010-2015 ($471,300; funded).
Principal Investigator, Administrative Supplement to R21 award “Identification of PKC delta
substrates in stroke-reperfusion injury” from NIH/NINDS 3R21NS057195-02S1, 2009 ($15,575;
funded).
Principal Investigator, “Identification of PKC delta substrates in stroke-reperfusion injury” from
NIH/NINDS R21 NS057195-01A1, 2007-2009 ($375,375; funded).
Principal Investigator, “Development of chemical-genetic approach”, Research Evaluation and
Allocation Committee (REAC) grant from the Dean of School of Medicine at UCSF, 2007-2008
($25,000; funded).
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7. Wen-Hai Chou, PhD
TEACHING EXPERIENCE
Courses Taught
Human Biology (3 credits, undergraduate course) at Kent State University: Fall 2011, Fall 2012,
Spring 2013, Fall 2013, Spring 2014, Fall 2014, Fall 2015
Bioenergetics (3 credits, graduate course) at Kent State University: Spring 2011, Spring 2012
Seminars in Physiology and Ecology (1 credit, graduate course) at Kent State University: Fall 2013,
Spring 2014
Research Methods in Neuroscience (1 credit, graduate course) at Kent State University: Fall 2011,
Fall 2012, Fall 2013
Individual Investigation (1-6 credit hours, depending on student schedule, perform research in my
lab, undergraduate course) at Kent State University: 2010-2015
Advanced Molecular Biology Laboratory (3 credits) at UT Health Science Center, 1994-1995
Introductory Biology Laboratory (1 credit) at Texas A&M University, 1990-1993
Laboratory Mentoring
Yi-Chinn Weng, Ph.D. of UTHSCSA, Senior Research Scientist at KSU, 2010-present
Guona Wang, M.D. of Taishan Medical University in China, Ph.D. student at KSU, 2011-present
Varun Kumar, M.S., B.S. of Delhi University in India, Ph.D. student at KSU, 2011-present
Layla Almutairi, Ph.D. student at KSU, 2015-present.
Fnu Vivek, took Internship in Biotechnology in the Spring of 2015
Supreet K. Sandhu, took Internship in Biotechnology in the Spring of 2015
Isabella da Cruz Franco, took Internship in Biotechnology in the summer of 2014
Scott Smith, undergraduate of KSU, took Individual Investigation at KSU, 2012-2014
Brandon Davis, undergraduate of KSU, took Individual Investigation at KSU, 2012-2014
Jacob Klamut, undergraduate of KSU, took Individual Investigation at KSU, 2012-2013
Samantha Stefl took Individual Investigation during 2012-2013
Melissa Lynch, undergraduate of KSU, took Individual Investigation at KSU, 2012-2013
James Whaley, undergraduate of KSU, took Individual Investigation at KSU, 2010-2013
Danielle Dauphars, undergraduate of KSU, took Individual Investigation at KSU, 2010-2011
Mohamed Ridha, undergraduate of KSU, took Individual Investigation at KSU, 2011-2011
Omar Nemer, undergraduate of KSU, took Individual Investigation at KSU, 2010-2011
Terrance Chiang, B.S. of UC Berkeley, Research Assistant at UCSF, 2009-2010
J. Eve Stoller, B.S. of UCLA, Research Assistant at UCSF, 2009
Maya Rosen, B.S. of Skidmore College, Senior Research Assistant at UCSF, 2007-2008
Dan Wang, M.D., M.S., Senior Research Specialist at UCSF, 2005-2010
Judith Wong, M.D. of UCSF, Research Assistant at UCSF, 2005-2006
Christi Wideman, B.S. of UTSA, Research Assistant at UTHSCSA, 1997-1998
Conference Proceedings
Kumar V., Wang, G., Weng, Y.-C., Chou, W.-H. (2015) Characterization of novel inhibitors for
analog-specific Protein Kinase C delta. The Society for Neuroscience 45th Annual Meeting,
Chicago, Illinois. (Poster)
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8. Wen-Hai Chou, PhD
Kumar V., Wang, G., Weng, Y.-C., Chou, W.-H. (2015) PKC epsilon in ischemia-induced
neurodegeneration. The 3rd KSU Neuroscience Research Symposium on Aging. (Poster)
Davis, B., Smith, S., Chou, W.-H. (2014) Developing a novel model of ischemic stroke using
magnetics. The 1st Undergraduate Symposium on Research, Scholarship and Creative Activity.
(Poster) (My undergraduate student, Brian Davis received the 2nd place poster award)
Wang, W., Weng, Y.-C., Han, H., Chou, W.-H. (2014) Lipocalin-2 is detrimental factor after stroke-
reperfusion injury. The 29th KSU Annual Graduate Research Symposium. (Poster) (My Ph.D.
student, Guona Wang received the best poster award)
Kumar V., Geldenhuys, W.J., Wang, D., Weng, Y.-C., Messing, R.O., Chou, W.-H. (2014) Structural
Characterization of ATP Analog-Sensitive Protein Kinase C Delta. The 29th KSU Annual
Graduate Research Symposium. (Poster)
Han, H., Weng, Y.-C., Wang, W., Chou, W.-H. (2014) NGAL and MMP-9 as potential biomarkers
for stroke. The 29th KSU Annual Graduate Research Symposium. (Poster)
Wang, W., Weng, Y.-C., Han, H., Chou, W.-H. (2014) Lipocalin-2 is detrimental factor after stroke-
reperfusion injury. The 2nd KSU Neuroscience Research Symposium on Obesity. (Poster)
Kumar V., Geldenhuys, W.J., Wang, D., Weng, Y.-C., Messing, R.O., Chou, W.-H. (2014) Structural
Characterization of ATP Analog-Sensitive Protein Kinase C Delta. The 2nd KSU Neuroscience
Research Symposium on Obesity. (Poster)
Han, H., Weng, Y.-C., Wang, W., Chou, W.-H. (2014) NGAL and MMP-9 as potential biomarkers
for stroke. The 2nd KSU Neuroscience Research Symposium on Obesity. (Poster)
Kumar V., Geldenhuys, W.J., Wang, D., Weng, Y.-C., Messing, R.O., Chou, W.-H. (2013) Structural
Characterization of ATP Analog-Sensitive Protein Kinase C Delta. The Society for Neuroscience
43rd Annual Meeting, San Diego, California. (Poster)
Wang, W., Weng, Y.-C., Han, H., Chou, W.-H. (2013) Lipocalin-2 is detrimental factor after stroke-
reperfusion injury. The Society for Neuroscience 43rd Annual Meeting, San Diego, California.
(Poster)
Wang, W., Weng, Y.-C., Han, H., Chou, W.-H. (2013) The role of NGAL in stroke. The 28th KSU
Annual Graduate Research Symposium. (Poster)
Kumar V., Weng, Y.-C., Chou, W.-H. (2013) The role of PKC epsilon in ischemia-induced
neurodegeneration. The 28th KSU Annual Graduate Research Symposium. (Poster) (My Ph.D.
student, Varun Kumar received the best poster award)
Chou, W.-H., Weng, Y.-C., Wang, G., Whaley, J.D., Kumar, V., Shokat, K.M., Messing, R.O. (2012)
Identification of Lipocalin-2 as a PKC delta Phosphorylation Substrate in Stroke-reperfusion
Injury. The Society for Neuroscience 42nd Annual Meeting, New Orlean, Louisiana. (Poster)
Chou, W.-H., Weng, Y.-C., Wang, G., Whaley, J.D., Kumar, V., Shokat, K.M., Messing, R.O. (2012)
Identification of Lipocalin-2 as a PKC delta Phosphorylation Substrate in Stroke-reperfusion
Injury. 12th Tiantan International Stroke Conference, Beijing, China. (Talk)
Chou, W.-H., Qi, Z.-H., McMahon, T., Wang, D., Messing, R.O. (2009) Regulation of GABAA
Receptor Trafficking by Protein Kinase C epsilon and NSF. Satellite Event (Synaptic Inhibition
in Health and Disease Conference) at The Society for Neuroscience 39th Annual Meeting,
Chicago, Illinois. (Talk)
Choi, D.-S, Lesscher, H.M.B., NAM, H., Chou, W.-H., Deitchman, J., McMahon, T., Wang, D.,
Kharazia, V.N., Messing, R.O. (2009) Amygdala protein kinase c delta suppresses anxiety-like
behavior. The Society for Neuroscience 39th Annual Meeting, Chicago, Illinoia. (Poster)
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9. Wen-Hai Chou, PhD
Chou, W.-H., Rosen, M., Wang, D., Allen, J.J., Shokat, K.M., Messing, R.O. (2009) Generation of
PKC delta - analog sensitive mutant to isolate direct substrates of PKC delta in stroke-reperfusion
injury. International Stroke Conference, San Diego, California. (Poster)
Chou, W.-H., Rosen, M., Wang, D., Allen, J.J., Shokat, K.M., Messing, R.O. (2008) Generation of
PKC delta - analog sensitive mutant to isolate direct substrates of PKC delta in stroke-reperfusion
injury. ASCB Annual Meeting, San Francisco, California. (Poster)
Chou, W.-H., Rosen, M., Wang, D., Allen, J.J., Shokat, K.M., Messing, R.O. (2008) Generation of
PKC delta - analog sensitive mutant to isolate direct substrates of PKC delta in stroke-reperfusion
injury. Hypoxia, Ischemia, and Inflammation: Essential Connections, Boston, Massachusetts.
(Poster)
Chou, W.-H., Wang, D., Allen, J.J., Wong, J., Shokat, K.M., Messing, R.O. (2007) Generation of
PKC delta - analog sensitive mutant to isolate its direct substrates in stroke-reperfusion injury.
The Society for Neuroscience 37th Annual Meeting, San Diego, California. (Talk)
Chou, W.-H., Wang, D., Song, M., McMahon, T., Messing, R.O. (2006) PKC epsilon regulates
GABAA receptor trafficking through NSF. FASEB, Experimental Biology, San Francisco,
California. (Poster)
Chou, W.-H., Wang, D., Song, M., McMahon, T., Messing, R.O. (2005) PKC epsilon regulates
GABAA receptor trafficking. The Society for Neuroscience 35th Annual Meeting, Washington
D.C. (Poster)
Wallace, M.J., Newton, P.M., Oyasu, M., McMahon, T., Chou, W.-H., Connolly, J., Messing, R.O.
(2005) Acute functional tolerance ethanol is mediated by Protein Kinase C epsilon. The Society
for Neuroscience 35th Annual Meeting, Washington D.C. (Poster)
Chou, W.-H., Choi, D.-S., Zhang, H., Mu, D., McMahon, T., Kharazia, V.N., Lowell, C.A., Ferriero,
D.M. and Messing, R.O. (2004) Neutrophil protein kinase C delta as a mediator of stroke-
reperfusion injury. The Society for Neuroscience 34th Annual Meeting, San Diego, California.
(Talk)
Britt, S.G., Chou, W.-H., and Jin, S. (2000) Novel genes required for photoreceptor cell patterning
and opsin expression in the Drosophila retina. The Gordon Research Conference on Visual
System Development. Roger Williams University, Bristol, Rhode Island. (Talk)
Chou, W.-H., Ray J. A., Lamar, R.M., and Britt, S.G. (1999) A69 and Sco, two mutations with
opposite phenotypes that disrupt an inductive signal between R7 and R8 photoreceptor cells. The
Eighth Biennial Cold Spring Harbor Meeting on Neurobiology of Drosophila, Cold Spring
Harbor Laboratories, Cold Spring Harbor, New York. (Talk)
Chou, W.-H., LaMar, R.M., and Britt, S.G. (1999) A69, a mutant with an abnormal pattern of opsin
expression in the R7 and R8 photoreceptor cells. The 40th Annual Drosophila Research
Conference, Bellevue, Washington. (Poster)
Salcedo E., Huber A., Bentrop J., Chadwell, L. V., Chou, W.-H., Paulsen, R., and Britt, S. G. (1999)
Blue- and green-sensitive visual pigments of Drosophila : Ectopic expression and physiological
characterization of the R8 photoreceptor cell-specific Rh5 and Rh6 rhodopsins. The 40th Annual
Drosophila Research Conference, Bellevue, Washington. (Poster)
Chou, W.H. and Britt, S.G. (1998) A developmental signal from the R7 cell that patterns the opsin
expression in the R8 cell. The Annual Graduate Student Association Forum, University of Texas
Health Science Center at San Antonio, Texas. (The first-place poster award)
Chou, W.-H., Huber, A., Schulz, S., Bentrop, J., Paulsen, R., and Britt, S.G. (1998) A binary switch
signal from the R7 cell that controls opsin expression in the R8 cell. The 39th Annual Drosophila
Research Conference, Washington D.C. (Talk)
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10. Wen-Hai Chou, PhD
Chou, W.-H., and Britt, S.G. (1997) Identification of a novel Drosophila opsin reveals specific
patterning of the R7 and R8 photoreceptor cells. The 38th Annual Drosophila Research
Conference, Chicago, Illinois. (Poster)
Invited Presentations
Department of Neurobiology, West Virginia University School of Medicine, “"PKC delta and
Lipocalin-2 in Stroke-reperfusion Injury ", 2015.
Department of Biochemistry, Kent State University, “"PKC signaling in cerebral ischemia and
reperfusion Injury ", 2015.
Department of Pharmaceutical Sciences, Northeast Ohio Medical University (NEOMED), “PKC
signaling pathways in stroke-reperfusion injury”, 2013.
Department of Biochemistry, Kent State University, “"Identification of PKC delta substrates in
stroke-reperfusion injury", 2012.
12th Tiantan International Stroke Conference, Beijing, China, “Identification of Lipocalin-2 as a PKC
delta phosphorylation substrate in Strok-reperfusion Injury”, 2012.
School of Biomedical Sciences, Kent State University, “PKCδ and PKCε in stroke-reperfusion
injury”, 2011.
Department of Biological Sciences, Kent State University, “Protein Kinase C in stroke-reperfusion
injury”, 2010.
Center for Translational Neuroscience, Case Western Reserve University, “Protein Kinase C in
Stroke-reperfusion Injury”, 2008.
Division of Surgical Research, Rhode Island Hospital, Brown University School of Medicine,
“Protein Kinase C in Stroke-Reperfusion Injury”, 2008.
Neural Injury and Repair seminar series, Neonatal Brain Disorders Center, Department of Neurology,
UCSF, “Protein Kinase C in Stroke-Reperfusion Injury”, 2007.
Center for Cerebrovascular Research, UCSF, “Protein Kinase C delta in Stroke-Reperfusion Injury”,
2005.
Division of Molecular and Cellular Medicine, National Health Research Institute, Taiwan, “Protein
Kinase C delta in Stroke-Reperfusion Injury”, 2005.
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, “Patterning of opsin expression in
Drosophila”, 2001.
Department of Entomology, National Chung-Hsing University, Taichung, Taiwan, “Patterning of
opsin expression in Drosophila”, 2001.
Society of Chinese Bioscientists in America, Houston, Texas, “Identification of a novel Drosophila
opsin reveals specific patterning of the R7 and R8 photoreceptor cells”, 1997.
SERVICE ACTIVITIES
University Level
Search committee for tenure-track faculty positions in biology at the Kent State University, Salem
Campus in Spring 2015.
Interviewer for the Integrated Life Sciences (BS/MD) program in 2012, 2013, 2014.
Open lab in the 2014 Spring Preview KSU Day and 2013 Summer Preview Day for recruiting new
undergraduate students.
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11. Wen-Hai Chou, PhD
Advising
Ph.D. co-advisor (with Dr. Min-Ho Kim) of Layla Almutairi, 2015-present
Ph.D. advisor of Guona Wang, 2011-present
Ph.D. advisor of Varun Kumar, 2011-present
Ph.D. dissertation committees of Pritam Sinha Roy, 2013-present
Ph.D. dissertation committees of Spencer Andrei 2013-present
Ph.D. dissertation committees of Sewwandi Rathnayake, 2011-present
Ph.D. dissertation committees of Amy Ionadi, 2012-present
Ph.D. dissertation committees of Teja Duduki, 2014-2015
Ph.D. dissertation committees of Lisa Zelinka, 2014
Ph.D. dissertation committees of Sabyasachi Sen, 2013-2015
Ph.D. dissertation committees of Suranjana Goswami, 2012-2013
Ph.D. dissertation committees of Shandilya Ramdas, 2011-2012
Grant Review
Cleveland Clinic Lerner Research Institute (CC-LRI) and Kent State University (KSU) Collaborative
Research Grants: Spring 2015
American Heart Association (AHA), Brain Stroke Study Sessions: three terms Spring 2013, Fall
2013, Fall 2014
Italian Ministry of Health, Republic of Italy (grant proposals): 2010-2012
Editorial Service
Elected as an Editor of Journal of Neurological Sciences and Psychiatry, 2015
Elected as a member of Editorial Board for Neuro Journal, 2014
Elected as a member of Editorial Board for Journal of Cytology & Molecular Biology, 2014
Ad Hoc referee for Brain Research, Journal of Neurochemistry, Journal of the Neurological Sciences,
Neuroscience Letters, Journal of Visualized Experiments, Journal of Neuroscience Research,
American Journal of Pathology, Austin Journal of Emergency and Critical Care Medicine, and
Reviews in the Neurosciences.
Other Professional Service
Member of Society for Neuroscience, 1998-present
Member of American Stroke Association, 2002-present
American Heart Association Council on Basic Cardiovascular Sciences - Council Fellow, 2011-
present
American Heart Association Council on Functional Genomics and Translational Biology - Council
Member, 2012-present
American Heart Association Stroke Council - Council Member, 2013-present
Member of American Society for Biochemistry and Molecular Biology, 2014-present
Member of American Society for Cell Biology, 2008-2009
Member of New York Academy of Sciences, 1999- 2008
Member of Society of Chinese Bioscientists in America, 1997-2008
Member of Genetics Society of America, 1996-2001
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