4. agile - affordable - accurate
Specifications
• Up to 60 samples/hour
• 15 images/sample
Throughput
• Storage of 10.000 measurements with 15
images each
Database
• Only 175 µl per measurement
• Low space requirement
Volume
• 2x Ethernet, 2x USB 3.0, 2x USB 3.1, 1x RS232
• Specified Serial interfaces for Barcode Reader,
• Chemical Analyzer and LIS
Connectivity
• Easy connection to urine test strip analyzer
Urilyzer® 100 Pro / 500 Pro
• Whole Urinalysis result report on one device
Compatibility
• Urilyzer® Cell cuvettes (closed system,
disposable) Pipetting tips
• No reagents needed
Consumables
9. agile - affordable - accurate
Connection to Chemical Analyzer
The
Urilyzer®
Cell can be
easily
connected to
the Urilyzer
100/500 Pro
Backside of
the Urilyzer®
Cell (CHEM)
via serial
cable to
chemical
analyzer
Urilyzer®
100 Pro: Go
into Main
Options
Settings
Output
LIS2 set
Baudrate
Urilyzer®
500 Pro: Go
into Main
Settings
Output
LIS2 set
Baudrate
Urilyzer®
Cell: Go into
Settings
Chemical
Analyzer
choose
„serial“ and
the
baudrate
Activate
The
chemical
analyser has
to be
measured
before the
Urilyzer®
Cell
Use the
same ID on
both devices
for each
sample
• Best with
barcode reader
(2 required)
10. agile - affordable - accurate
Workflow – Manual procedure
Pressing
the Start
button to
load an
empty
cuvette to
pipetting
position
Barcode
scanning
Sample
mixing
Aspirating
urine
sample
using
manual
pipette
Injecting
urine
sample into
cuvette
Pressing
the Start
button to
start the
measurem
ent
11. agile - affordable - accurate
Workflow – Automated procedure
Centrifuging process
Results are forwarded to Data
Manager Software
Cuvette is moved to next
microscope position
Focusing process
Cuvette is placed into first
microscope position
Recorded image is evaluated
HPF-like image is taken by
built-in camera
Used cuvette is placed into the
waste bin
Last image?
NO
YES
12. agile - affordable - accurate
agile - affordable - accurate
Pipetting practice
14. agile - affordable - accurate
Workflow – Manual procedure
• Press the Start-Button on top of the device
Pressing the Start button: to
load an empty cuvette to
pipetting position
Barcode scanning Sample mixing
Aspirating urine sample using
manual pipette
Injecting urine sample into
cuvette
Pressing the Start button: to
start the measurement
15. agile - affordable - accurate
Workflow – Manual procedure
• Press the Start-Button on top of the device
• Enter ID - either manual or by a barcode scanner
Pressing the Start button: to
load an empty cuvette to
pipetting position
Barcode scanning Sample mixing
Aspirating urine sample using
manual pipette
Injecting urine sample into
cuvette
Pressing the Start button: to
start the measurement
16. agile - affordable - accurate
Workflow – Manual procedure
• Press the Start-Button on top of the device
• Enter ID - either manual or by a barcode scanner
• Invert the sample several times – do not shake!
Pressing the Start button: to
load an empty cuvette to
pipetting position
Barcode scanning Sample mixing
Aspirating urine sample using
manual pipette
Injecting urine sample into
cuvette
Pressing the Start button: to
start the measurement
17. agile - affordable - accurate
Workflow – Manual procedure
• Press the Start-Button on top of the device
• Enter ID - either manual or by a barcode scanner
• Invert the sample several times – do not shake!
• Pick a pipette with 175 µl sample volume and aspirate the sample
• Outer needle diameter of 1,2 – 2,8 mm
Pressing the Start button: to
load an empty cuvette to
pipetting position
Barcode scanning Sample mixing
Aspirating urine sample using
manual pipette
Injecting urine sample into
cuvette
Pressing the Start button: to
start the measurement
18. agile - affordable - accurate
Workflow – Manual procedure
• Press the Start-Button on top of the device
• Enter ID - either manual or by a barcode scanner
• Invert the sample several times – do not shake!
• Pick a pipette with 175 µl sample volume and aspirate the sample
• Outer needle diameter of 1,2 – 2,8 mm
• Fill the sample into the cuvette
Pressing the Start button: to
load an empty cuvette to
pipetting position
Barcode scanning Sample mixing
Aspirating urine sample using
manual pipette
Injecting urine sample into
cuvette
Pressing the Start button: to
start the measurement
19. agile - affordable - accurate
Workflow – Manual procedure
• Press the Start-Button on top of the device
• Enter ID - either manual or by a barcode scanner
• Invert the sample several times – do not shake!
• Pick a pipette with 175 µl sample volume and aspirate the sample
• Outer needle diameter of 1,2 – 2,8 mm
• Fill the sample into the cuvette
• Press the Start-Button again to start the measurement process
Pressing the Start button: to
load an empty cuvette to
pipetting position
Barcode scanning Sample mixing
Aspirating urine sample using
manual pipette
Injecting urine sample into
cuvette
Pressing the Start button: to
start the measurement
20. agile - affordable - accurate
Workflow – Automated procedure
• Creating a monolayer of particles by
centrifuging the cuvette
10 seconds with 2000 RPM
Centrifuging
process
Results are
forwarded to Data
Manager Software
Cuvette is
moved to next
microscope
position
Focusing
process
Cuvette is placed
into first
microscope
position
Recorded
image is
evaluated
HPF-like image
is taken by
built-in camera
Used cuvette is
placed into the
waste bin
Last image?
NO
YES
21. agile - affordable - accurate
Workflow – Automated procedure
• Creating a monolayer of particles by
centrifuging the cuvette
• Cuvette is moved above microscope
Centrifuging
process
Results are
forwarded to Data
Manager Software
Cuvette is
moved to next
microscope
position
Focusing
process
Cuvette is placed
into first
microscope
position
Recorded
image is
evaluated
HPF-like image
is taken by
built-in camera
Used cuvette is
placed into the
waste bin
Last image?
NO
YES
22. agile - affordable - accurate
Workflow – Automated procedure
• Creating a monolayer of particles by
centrifuging the cuvette
• Cuvette is moved above microscope
• Focus series is captured in different
heights
Centrifuging
process
Results are
forwarded to Data
Manager Software
Cuvette is
moved to next
microscope
position
Focusing
process
Cuvette is placed
into first
microscope
position
Recorded
image is
evaluated
HPF-like image
is taken by
built-in camera
Used cuvette is
placed into the
waste bin
Last image?
NO
YES
23. agile - affordable - accurate
Workflow – Automated procedure
• Creating a monolayer of particles by
centrifuging the cuvette
• Cuvette is moved above microscope
• Focus series is captured in different
heights
• Camera takes a picture of the
sharpest plane
Centrifuging
process
Results are
forwarded to Data
Manager Software
Cuvette is
moved to next
microscope
position
Focusing
process
Cuvette is placed
into first
microscope
position
Recorded
image is
evaluated
HPF-like image
is taken by
built-in camera
Used cuvette is
placed into the
waste bin
Last image?
NO
YES
24. agile - affordable - accurate
Workflow – Automated procedure
• Creating a monolayer of particles by
centrifuging the cuvette
• Cuvette is moved above microscope
• Focus series is captured in different
heights
• Camera takes a picture of the
sharpest plane
• The system checks if this was the last
picture
• The image gets automatically
evaluated and is saved in the
database
Centrifuging
process
Results are
forwarded to Data
Manager Software
Cuvette is
moved to next
microscope
position
Focusing
process
Cuvette is placed
into first
microscope
position
Recorded
image is
evaluated
HPF-like image
is taken by
built-in camera
Used cuvette is
placed into the
waste bin
Last image?
NO
YES
25. agile - affordable - accurate
Workflow – Automated procedure
• The system checks if this was the last
picture
• YES: The cuvette is moved into the
waste bin
• NO: the cuvette position moves to
next microscope position
Centrifuging
process
Results are
forwarded to Data
Manager Software
Cuvette is
moved to next
microscope
position
Focusing
process
Cuvette is placed
into first
microscope
position
Recorded
image is
evaluated
HPF-like image
is taken by
built-in camera
Used cuvette is
placed into the
waste bin
Last image?
NO
YES
28. agile - affordable - accurate
Auto classification
Particle type:
Subclasses and other particles
can be added
Semi-quantitative category:
The ranges and category name
can be changed
Quantitative results:
p/µl or p/HPF
Negative result limits
RBC and WBC are real quantitative results
Every other result is semi-quantitative even
if the result is displayed quantitative
30. agile - affordable - accurate
Focussing process
• 2000 steps ≡ 2,5 mm
• 1 step ≡ 1,25 µm
• Hard Focus Start ~ 800
• Set with the Reference Cuvette in
Service Software
2000 steps
Pic. 1
800
}320 steps
Taking 1 image every two steps
chooses the best
Pic. 2 Pic. 3
} 16 steps up &
16 steps down
} 16 steps up &
16 steps down
Best image heigth of the
previous one defines the start
taking 16 images in total and
choses the best again
Best image heigth of the
previous one defines the start
taking 16 images in total and
choses the best again
And so on with the remaining 12 images
31. agile - affordable - accurate
Manual addable sediments
• Up to 50 parameters
are manual addable
• The red ones are only
manual addable
• Can be used for
further information
• Parameters must be
activated to add them
32. agile - affordable - accurate
Review criteria
0-2 review images normal measurement:
• The results are calculated based on the non-review images can be
auto-validated
≥ 3 review images and ≥ 5 O.K. images: review measurement:
• The results are calculated based on the non-review images cannot
be auto-validated
< 5 O.K. images: review measurement:
• No automated result cannot be auto-validated
• User can modify the final sample results based on the manual review
of the images or remeasure the sample with a dilution
MUC review:
• Concentration limit can be set for MUC: if the sample concentration is
above this limit, the measurement will be flagged for review
• The results are calculated based on the images cannot be auto-
validated
33. agile - affordable - accurate
Maintenance
• Daily routine should maintain a
cleaning process after every workshift,
24 hours or according to the local
regulations
• What should be cleaned:
• Cuvette feeder arm
• Cuvette hold down assembly
• Drop tray
• Centrifuge cover
• Centrifuge arm
• The path of the cuvette, especially the
top of the microscope
• Waste bin
• The outer surface, especially the
pipetting window
34. agile - affordable - accurate
agile - affordable - accurate
We look forward to the opportunity to work with you.
