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Axel Hausmann - Invertebrates Plenary
1. How to assess the ‘Megabiodiversity’ of Invertebrates:
55% of the world’s geometrid species
are DNA barcoded
Axel Hausmann(1), Rodolphe Rougerie(2), Paul D. N. Hebert(3)
(1) Zoologische Staatssammlung München, Germany (2) Université de Rouen, France (3) Biodiversity Institute of Ontario, University of Guelph, Canada
2. The clock is ticking …
Global disasters:
continuous loss of radioactivity
27. Reliably identified collection material
versus fresh samplings
Europe & North America (2000)
ANIC Australia (2000)
coll. Staude, ZA (1000)
Rest
(13,000)
coll. Herbulot, world (5000)
30. Selection
of study
sites:
Ghana,
Kakum NP
Eudaemonia argus
Canopy Access
by Walkway
31. Sampling under tropical conditions
Ammonia syringe
Pinned in the field
(Larger specimens in
Killing jar small envelopes)
Back home: Labelling & leg sampling
Barcode success rate: >95%
Relaxing (max. 2 days) & spreading later on!
34. A priori identification
of samples from tropical countries
to (sub)family
(e.g. ‘Larentiinae')
5% to species
20% (e.g. ‘Eois aurata')
45%
to genus
30%
(e.g. ‘Eois AH01Ge')
to species group, tentatively
(e.g. ' Eois aurataAH01Ge')
42. Continental assessments European campaign
pending
9%
barcoded
European geometrids
91%
total of 970 species (Hausmann 2007)
barcoded by April 2011: 880 species (91 %)
accessible on BOLD with image and georeferencing
47. Continental assessments African geometrids
goal: 2.8-3K species (almost complete)
data release publication late 2012 (PLoS one)
48. Library of Life – made in Germany
Data release geometrids of Bavaria
after less than 2 years
406 of 408 species barcoded (99%)
1815 specimens, 1393 barcodes
51. Synonymy
Kuchleria garciapitai Exposito, 2005 syn.n. to
Kuchleria insignata Hausmann, 1995
TAXONOMY
Kuchleria insignata Kuchleria garciapitai
Holotype, E Spain from type locality, C Spain
52. Variation within species
70
Variation (maximum pairwise distance) within species
>2%
1-2%
60
0-1%
50
40
30
20
10
0
0-0,5 1-1,5 2-2,5 3-3,5 4-4,5 5-5,5 6-6,5 7-7,5 8-8,5 9-9,5 10-10,5 11-11,5 12-12,5 13-13,5
5 % with cryptic diversity in COI (15+5 species, >2%)
9/20 with double clusters, 11/20 with out-sticking singletons
55. A new pest for the Fauna of North
Africa: The winter moth O. brumata
PEST CONTROL
photo: E. Friedrich
map: BOLD database
56. Agents for the control of invasive plants
Eueupithecia cisplatensis for the control
QUARANTAINE
of Parkinsonia aculeata in Australia
photos: CSIRO, Australia by the courtesy of Tim Heard
57. ECOLOGY Agents for the control of invasive plants
58. ECOLOGY Analysis of gut content
COI Sequence
unknown larva (Dead Sea)
Identification moth:
Semiothisa aestimaria
59. ECOLOGY Analysis of gut content
matK/rbcL Sequence COI Sequence
unknown larva (Dead Sea)
Identification plant: Identification moth:
Tamarisk Semiothisa aestimaria
60.
61.
62.
63.
64. How to assess the ‘Megabiodiversity’ of Invertebrates:
63% of the world’s geometrid species
are DNA barcoded
Funding agencies
Acknowledgements
Paul Hebert for a wonderful and successful partnership!
Rodolphe Rougerie, University of Rouen for iBOL 1.9 co-lead
Sujeevan Ratnasingham (BOLD), and the teams of BIO and CCDB
BFB-team: G. Haszprunar, S. Schmidt, M. Balke, L. Hendrich, A. Segerer
Bayerisches Staatsministerium für Wissenschaft und Kunst
Hundreds of enthusiastic and active partners all over the world