2. By,
Ms: Ayeeshya Kolhar
I.D. No: UHS17PGD223
Dept of Post Harvest Tech
UNIVERSITY OF HORTICULTURAL
SCIENCES BAGALKOT
COLLEGE OF HORTICULTURE, BAGALKOT
11. 5/10/2018
Definition
Fortification means deliberately increasing the
content of the essential micronutrients in the food so
as to improve the nutritional quality of the food
Fortification
Fortified foods
Foods to which extra nutrients have been added
FSSAI, 2017
12. Functional foods
Foods which are specially processed or formulated to
satisfy particular dietary requirements which exist because
of a particular physical or physiological condition or specific
diseases and disorders and which are presented as such,
wherein the composition of these foodstuffs must differ
significantly from the composition of ordinary foods
(FSSA, 2006)
5/10/2018
Improved state of health and well being
/ reduction of risk of disease
13. Importance of functional foods
Best tool to fit for global prevalence of micronutrient
malnutrition
Improves public health
Generates employment and income
Provides balanced diet
Best suited for present lifestyle
5/10/2018
Mishra .,2016
14. Types of Food Fortification
The 4 main methods of food fortification
Biofortification
Microbial Biofortification and synthetic biology
Commercial and industrial fortification
Home fortification
5/10/2018 Pant and Chinwan., 2014
15. Purpose of food fortification
Improve nutritional quality of food
Reduce nutritional disorders
Fortification for body building
Fortification for medical treatment
5/10/2018 Pant and Chinwan., 2014
16. Objectives
• To maintain the nutritional quality of foods.
• Keeping nutrients levels adequate to correct or prevent specific
nutritional deficiencies in the population or in groups at risk of
certain deficiencies.
• To increase the added nutritional value of a product (commercial
view).
• To provide certain technological functions in food processing.
5/10/2018
17. Advantage of fortification
socially acceptable.
fast and broad.
organoleptic properties.
safest strategy
cost effective approach
marketing and distribution system.
readily visible.
In addition, fortification will reach secondly target risk groups,
such as the elderly, the all and those who have an unbalanced
diet.
5/10/2018
18. Limitations of fortification
• A thorough knowledge of dietary habits and nutrient intake in
the target group(s).
• A complementary educational programme is required
particularly when the fortification influences organoleptic
characteristics of the food.
• Food fortification is not the ultimate solution of a nutritional
deficiency
5/10/2018
19. Criteria for fortification
Part of the regular daily diet
Provide an effective supplement
Not harmful to high consumers
Sensory properties
Cost should be economical
5/10/2018
FAO Food and nutrition ., 1996
20. Food fortification- Technology
Food based strategies are most effective and sustainable approach to
combat macro and micronutrients deficiencies.
i. Selection of appropriate fortificant
Target groups
High consumers
Affordable and available all year long
Organoleptic properties
ii. Technically and economically feasible
FAO Food and nutrition ., 1996
21. Examples of fortified foods:
• Milk with Vitamin D
• Salt with Iodine
• Fruit juice with Calcium
• Water or toothpaste with fluoride
• Flour with Folic Acid
• Bread with Niacin
5/10/2018
23. Why food fortification?
Restoration of natural levels
Fortification above natural levels
Infant foods
Geriatric foods
Products designed for weight control
Public health objectives
Self sufficient
5/10/2018
24. Table 2. Fortification programme around world
Country Fortification
Egypt Iron and Folic acid with bread
South Africa
Folic acid with maize meal and
wheat flour
China Iron with Soy sauce
India
Salt with iodine and Iron ,
Oil with Vit-A/E
Atta with Folic acid and Iron,
Juices with Vit C, Vit D, Calcium
United States
Iodine with salt
Niacin with bread products
5/10/2018 :http://www.who.int/nutrition/en
25. General principles for fortification
1) Essential nutrients may be appropriately added to foods for the
purpose of
a) Preventing and reducing the risk of demonstrated deficiency
b) One or more essential nutrients
c) Health and nutritional quality of foods
2) If mandatory, it shall be based on severity and extent of public
health need as demonstrated by accepted scientific evidence.
3) Fortification in staple food based on the directions of
Government of India.
5/10/2018 FSSAI, 2017
27. Balance diet
A diet which provide all the essential nutrient in sufficient
quantities to meet the need of an individual is an adequate and
balance diet.
RDA ( recommended dietary allowance is the estimates of
intake of nutrients , in which population groups need to
consume to meet the physiological needs of all persons in the
group.
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30. Why do we need fortified foods?
Increased health and diet
Illness and disease/disorder
Present lifestyle
Public health intervention
Replace lost nutrients
Nutritional equivalence
Appropriate vitamin and minerals
5/10/2018
31. Approaches to Fortified Foods
Product Formulation
Novel Processing
Modification of Raw Materials
5/10/2018
32. Table 3. Classification of functional components
Sl. No Class Example
1 Inorganic mineral supplements Minerals
2 Probiotics Helpful bacteria
3 Prebiotics Digestive enzymes
4 Dietary fibers Fibers
5 Antioxidants Natural antioxidants
6 Phytochemicals
Fatty acids Omega3 fatty acids
Phenolics Wine and tea phenols
Lipids Sphingolipids
Proteins Soy proteins
7 Herbs Herbal drinks
5/10/2018 Abuajah et.al.,2014
36. Fortification of orange juice with vitamin D: a novel
approach for enhancing vitamin D nutritional health
Tangpricha et. al., (2003)
Case study – 1
Department of Medicine, Boston University
School of Medicine, Boston.
American Society for Clinical Nutrition 2;77:1478–83.
5/10/2018
37. Subject and Methods
• All study subjects gave written informed consent for
participation in the studies.
• Subjects: Thirty adults with an average age of range : 22–60
y) were recruited for this study.
• Protocol : The orange juice was given to each subject . One
group consumed 240 mL orange juice fortified with 350 mg
Ca and the other group consumed 240 mL orange juice
fortified with 350 mg Ca and 1000 IU vitamin D3, daily for
12 wk.
• A blood sample was obtained weekly from each subject for
measurement of serum 25(OH)D.
5/10/2018 Tangpricha et. al., (2003)
38. 5/10/2018
Fig. 3: Serum 25-hydroxyvitamin D [25(OH)D] concentrations in
subjects who ingested vitamin D–fortified orange juice at
concentration of 1000 IU ( ▄ ) and unfortified orange juice (●)
Tangpricha et. al., (2003)
39. Inference
The subjects who consumed the vitamin D3–fortified
orange juice had significantly higher 25(OH)D
concentrations at the end of the study compared with
the control subjects and also had greater increases
from baseline 25(OH)D concentrations.
5/10/2018 Tangpricha et. al., (2003)
40. Studies on Preparation of Fortified Sapota-Papaya
Fruit Bar
Kumar et al., ( 2012)
Case study – 2
Nutrition & Food Sciences 2012 2 (6) 1-3.
Objective: To study the effect of skim milk powder on nutritional
quality of sapota- papaya fruit bar5/10/2018
41. Materials and Methods
Development of fruit bar : fruits are procured
from the local market for the development of fruit
bar.
Pulp was extracted from the peeled fruits with the
help of pulper.
To the boiled pulp Sugar(50%),Pectin (2.5%),
Citric acid (1%), Maltodextrin (1%) and SMP(0-
6%) were added and it was heated enough to form
homogenous mixture.
The mixture was poured into Aluminium trays
(smeared with butter) in thin layer (0.5-1 cm) and
dried at 60 ± 2°C for 10-12 h intray drier
5/10/2018
Kumar et al., ( 2012)
42. Table 4: Optimization of ingredients in fruit bar.
Ingredients A B C D E
Sapota pulp (g) 100 80 70 60 50
Papaya pulp (g) 00 20 30 40 50
Pectin (g) 0.90 1.20 1.50 1.80 2.10
Skim Milk
Powder(g)
2 4 6 8 10
5/10/2018
Kumar et al., ( 2012)
45. Table 7. Effect of SMP fortification on quality of sapota-
papaya fruit bar
Parameter K(0%SMP) K(2%SMP) L(4%SMP) M(6%SMP)
Moisture (%) 15.91 15.55 15.87 14.64
Protein (%) 0.87 1.17 1.61 1.85
Fat (%) 3.08 3.01 3.04 2.9
Total Ash (%) 2.2 2.31 2.40 2.47
Crude Fiber (%) 10.22 11 12.02 12.08
Carbohydrate (%) 77.94 77.96 77.08 78.14
Total sugar (%) 15.51 16.21 16.49 16.97
Reducing sugar (%) 6.93 8.57 8.91 9.41
Non-reducing sugar % 8.58 7.64 7.58 7.56
Ascorbic Acid (mg/100g) 171 178 184 205
Total Energy (Kcal) 342.96 343.61 342.12 346.06
5/10/2018
Kumar et al., ( 2012)
46. Inference
With regards to the organoleptic qualities, all the fortified fruit
bar were excellent with nutritional quality particularly protein,
fat, crude fibre and calorie content increased with increasing
SMP (0-6%) in fruit bar.
Supplementing the value added sapota-papaya fruit bar to
preschool children and adolescents will be a promising effort
to reduce malnutrition.
5/10/2018
Kumar et al., ( 2012)
47. Effectiveness of Some Fortified Nutritional Products
with Sun Dried Banana Peels on Moody Status of
Faculty Education Students in Nujran
Gouda. ( 2017)
Case study – 3
Department of Home Economics, Fayoum University,
Fayoum, Egypt
Objective: To know effectiveness of some fortified nutritional products with
dried banana peels on moody status of faculty of education students.
5/10/2018
48. Materials and Methods
Materials
Banana peels.
Wheat flour extraction (70% to 72%).
White sugar, salt, butter, eggs.
Thirty students from faculty of education.
Sensory evaluation.
5/10/2018 Gouda. ( 2017)
49. Preparation of raw materials
• Banana peels powder
• Preparation of supplemented products with banana peels
Three types of food products (Cake, Biscuit and cookies) were
supplemented with dried banana peels (DBP) at level (20%).
Formulas consisted of 80 and 100 gm of flour, 25 gm sugar,
one egg and 25 gm butter, half cup milk,
Replaced 20% wheat flour (WF) with 20% DBP (Dried
Banana Peels).
Flour and other ingredients were mixed and the dough at room
temperature for 15 min, it was leaved 10 minutes, formed, and
baked at 200°C for 10 minutes in an electric oven
5/10/2018 Gouda. ( 2017)
50. Samples
Matters
Cake Biscuit Cookies
Formula
1 (0)
Formula
2 (20%)
Formula
1 (0)
Formula
2 (20%)
Formula
1 (0)
Formula
2 (20% )
WF (g) 100 80 100 80 100 80
BP (g) 0 20 0 20 0 20
Butter (g) 25 25 25 25 25 25
Sugar (g) 25 25 25 25 25 25
Salt (g) 5 5 5 5 5 5
Milk (ml) 50 50 0 0 0 0
Eggs 1 1 1 1 1 1
Vanilla (g) 5 5 5 5 5 5
Baking powder
(g)
7 7 7 7 7 7
WF( wheat flour) , BP ( banana peel)
5/10/2018
Table 8: Formulas of (Cake, biscuit and cookies) at level (20%) of
DBP(g/100 g)
Gouda. ( 2017)
51. 5/10/2018
Fig 1: Sample of control cake and fortified with (20%) banana peels.
Fig 2: Sample of control biscuit and fortified with 20% banana peels
Fig 3: Sample of control cookies and fortified with 20% banana peels
52. Table 9: Mean values of sensory evaluation of supplemented products
with DBP and control sample
Sensory
evaluation
Samples
Cake Biscuit Cookies
Control
Fortified
cake with
20% Control
Fortified
Biscuit with
20%
Control
Fortified
Cookies
with
20%
Taste 4.5 5 4.5 5 5 5
Color of crust 5 4.5 5 4.5 5 5
Color of pulp 5 5 4.5 5 5 4.5
Flavor 4.5 5 5 4.5 5 5
Texture 5 5 5 4.5 4.5 5
Overall
acceptability
4 4.5 4 4.5 4.5 4.5
Mean 4.8 5.9 4.8 4.7 5.9 4.7
5/10/2018
Gouda. ( 2017)
56. Table 13: Chemical analysis of total antioxidants and total
phenolic compounds in BP compared with banana fruit.
Parameters BF mg/100 g BP mg/100 g
Total antioxidants 37.98 ± 2.89 91.05 ± 1.69
Total phenolic 43.96 ± 0.3 65.36 ± 1.53
5/10/2018
Gouda. ( 2017)
57. Inference
Banana peel is rich in minerals such as (K, P, Ca, Fe and Mg),
vitamins (A, B complex, E and C).banana peels could be used
as supplementing of some bakery products to improve
nutritional quality, mood and treatment depression.
the sensory evaluation of supplemented food (Cake, Biscuit
and Cookies) with dried banana peels was accepted and it is
useful for improving the mood and depression.
5/10/2018
Gouda. ( 2017)
58. Probiotics
Probiotics
The living organisms that provide health benefits in the host when
consumed in the appropriate quantity ( FAO and WHO, 2008 )
• Bifidobacterium
• Lactobacillus
• Yeast- Saccharomyces
5/10/2018
59. Key properties of probiotic:
Non-pathogenic, non-toxic and non-allergic.
Capable of surviving and metabolizing in upper G.I. tract e.g.
Resistant to low pH, organic acids, bile juice, saliva and gastric acid
Able to modulate immune response and provide resistance to disease
through improved immunity or by the production of antimicrobial
substance in the guts.
Good adhesion/ colonization to human intestinal tract and influence
on gut mucosal permeability.
5/10/2018
60. Antagonistic against carcinogenic/ pathogenic organisms.
Clinically proven health benefit, e.g. gastrointestinal disorders,
diarrhoea, clostridium difficle colitis, antibiotics associated
diarrhoea, acute gastroenteritis.
Technologic properties for commercial viability such as stability of
desired characteristics during processing, storage and transportation.
5/10/2018
62. Probiotic products marketed in India
India is an emerging market for probiotic
products, being the largest producer of milk.
Amul , Mother dairy, Nestle Nesvita (first India’s
Dahi with probiotics) and Yakult are the major
brands.
63. Prebiotics
Non-digestible substances that provide a beneficial physiological
effect for the host by selectively stimulating the favorable growth
of a limited number of indigenous bacteria
• Oligofructose
• Inulin
• Galacto-oligosaccharides
• Lactose
5/10/2018
65. Sensory Characteristics and Consumer Acceptance
of Fruit Juice Containing Probioitcs Beads in
Thailand
Krasaekoopt and Kitsawad (2010)
Case study – 4
Faculty of Biotechnology, Assumption
University Bangkok, Thailand
AU J.T. 14(1): 33-38 (Jul. 2010)
5/10/2018
66. Materials and Methods
• Lactobacillus casei 01 was inoculated into 10 mL de Man
Rogosa and Sharp (MRS) broth and incubated at 37 °C for 2
days under aerobic condition.
• The culture was transferred to 95 mL MRS broth and
incubated at the same condition. The cells were harvested by
centrifuging at 1,500 x g for 15 min at 25 °C. The harvested
cells were washed twice with sterile water.
• Microencapsulation of probiotics
• Application of Microencapsulated Probiotic Beads in Fruit
Juice
• Sensory Evaluation
5/10/2018 Krasaekoopt and Kitsawad (2010)
67. Fig.4: Consumers hedonic rating on qualities of fruit juice used for purchasing the
product
5/10/2018 Krasaekoopt and Kitsawad (2010)
68. Fig. 3: Percentages of consumer opinion on fruit juice
containing probiotic beads
5/10/2018 Krasaekoopt and Kitsawad (2010)
69. Table 14: Mean of texture, overall scores and consumer
acceptance for fruit juice with probiotic beads
Product
Texture score Overall score Acceptance (%)
Orange juice
6.6 a 6.7 a 82.3 a
Grape juice
6.8 a 6.9 a 84.3 a
5/10/2018 Krasaekoopt and Kitsawad (2010)
70. Inference
fruit juice containing probiotic beads is a new product to the
consumers which can improve nutritional quality of fruit juice
a good number of consumers accepted orange and grape juices
containing probiotic beads.
5/10/2018 Krasaekoopt and Kitsawad (2010)
71. Physico-chemical analysis Of fresh and probioticated
fruit juices with Lactobacillus casei
Kuma et al., ( 2013)
Case study – 5
Department of Biotechnology,
S.V.University, Tirupati - 517502, India
International Journal of Applied Sciences and
Biotechnology
5/10/2018
72. Lactobacillus casei 1423 was obtained from MTCC
Grown at 37oC for 24 h in MRS broth and used as a innoculum
Mango, sapota, grape (white and black) and cantalope
fruits were collected
Fruits were peeled with the help of sharp knife and cut
into small pieces and then pulped
The juice samples were subjected to analysis
Kumar et al., 2013
MTCC: Microbial Type Culture Collection
MRS: Man Rogosa and Sharp
Material and methods
5/10/2018
73. Table 15: Qualitative analysis of various fruit juices for
phyto-chemical composition
Components Mango Sapota White grape Black grape Cantalou
pe
Carbohydrates + + + + +
Flavoniods + + + + +
Tannis + + + + +
Glycosides + + + + +
Alkaloids _ _ _ _ _
Saponins _ _ _ _ _
5/10/2018 Kuma et al., ( 2013)+ ( present) , - ( absent )
74. Table 16: Physico-chemical properties of freshly
prepared fruit juices
Name of
the fruit
Juice yield
(ml/kg)
Total soluble
solids
(Brix %)
Titratable
acidity (%)
pH Reducing
sugars %
Mango 500 20.3±0.81 0.3±0.1 4.4 18.1±1.2
Sapota 450 19.1±0.76 0.15±0.01 5.5 16.1±1.0
Grape (White) 500 15±1.0 0.14±0.01 4.6 9±1.0
Grape (Black) 550 19.3±0.8 0.17±0.01 4.5 11±1.0
Cantaloupe 400 11±1.0 0.13±0.01 4.0 10.3±1.5
5/10/2018 Kuma et al., ( 2013)
75. Table 17: Physico-chemical analysis of various
probioticated fruit juices at different intervals
Name of the fruit
juice
Time
intervals (h)
TSS Brix % Titrable acidity pH Reducing
Sugars
Mango 24 20±1.1 0.70±0.01 3.8 10.7±0.58
48 18±1.0 0.66±0.07 3.4 5.3±0.10
72 11±1.0 0.39±0.01 3.2 3.1±0.11
Sapota 24 19±1.3 0.42±0.06 5.0 13.1±0.60
48 16±1.0 0.46±0.01 4.5 10.3±0.75
72 13±1.2 0.49±0.01 4.3 5.5±0.81
Black grape 24 18±1.3 0.17±0.06 4.4 10.1±1.0
48 16±1.0 0.22±0.01 4.2 8.3±0.73
72 14±1.2 0.28±0.01 4.0 5.5±0.85
Cantaloupe 24 10±1.0 0.16±0.01 4.5 5.4±0.71
48 9.3±1.5 0.17±0.02 4.3 3.6±0.05
72 6±1.0 0.42±0.01 4.1 4.1±0.06
5/10/2018 Kuma et al., ( 2013)
76. Conclusion
Based on the above results, it was inferred that the beverage
prepared from the probiotication of fruit juices with
Lactobacillus casei could be useful in providing health
benefits to the consumers.
5/10/2018 Kuma et al., ( 2013)
78. Global regulatory authorities on fortified foods
United States Food and Drug Administration (USFDA)
World Health Organization (WHO)
Food Safety and Standards Authority of India (FSSAI) and Ministry of Food Processing
Industry (MOFPI) for India
European Food Safety Authority for European Union
5/10/2018
79. Ministry of Health, Labour and Welfare
for Japan (MHLW)
Food Fortification Resource Centre,
New Delhi (FFRC)
5/10/2018
80. Conclusion
Vitamin D fortification at 1000 IU/240 mL orange juice for 12 week safely
increased 25(OH)D3 concentrations in adults.
developed fruit bar fortified with 6% SMP was found to be rich in protein ,
fat, crude fibre and calorie and it can be given as a supplement to
malnutrition.
fruit peels as banana peels could be used as supplementing of some
bakery products as cake or biscuit with chocolate and cookies as
clinical nutrition to improve mood and treatment depression
Fruit juice containing probiotic beads is an innovative product the
beneficial health effects obtained from this product are invaluable; so the
consumers may be willing to pay more for a better quality of the products.
the beverage prepared from the probiotication of fruit juices with
Lactobacillus casei could be useful in providing health benefits to the
consumers.
5/10/2018
81. Probably no other technology available today offers as large
an opportunity to improve lives and accelerate development
at such low cost and in such a short time
as the fortification of staple
can do……
THANK YOU5/10/2018
Editor's Notes
MTCC(Microbial Type Culture Collection) MRS (Man Rogosa Sharpe)